scholarly journals Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats

2020 ◽  
Vol 10 (1-s) ◽  
pp. 174-177
Author(s):  
Ikenna Kingsley Uchendu ◽  
Ebuka Bitrus Nnedu ◽  
Ifeoma Blessing Ekeigwe
Keyword(s):  
Histopathological Examination ◽  
Nigella Sativa ◽  
Intraperitoneal Administration ◽  
Negative Control ◽  
Seed Extract ◽  
Albino Rats ◽  
High Dose ◽  
Protective Effects ◽  
Oral Group ◽  
Nigella Sativa Seed

Objective:  The aim of this study is to investigate the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cardiotoxicity of cadmium in albino rats.  Methods: Twenty five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Cardiac injury was assessed by measuring serum levels of Aspartate aminotransferase (AST), Lactate dehydrogenase (LDH) and Creatine kinase (CK-MB) using standard methods. The heart was harvested for histopathological examination. Results: CdCl2 induced significant cardiotoxicity with marked elevation in the levels of biochemical markers of cardiac functions (p<0.05 or p<0.01); these were however attenuated by MENS. Histopathological examination of the heart sections supported the biochemical findings. Conclusion: Nigella sativa seed extract is potentially cardioprotective against harmful chemical toxins such as cadmium. Keywords: Cadmium, CdCl2, Nigella sativa, Medicinal food, cardiotoxicity

Nigella sativa Seed Protects Against Cadmium-induced Renal Toxicity in Rats

2020 ◽  
Vol 14 (2) ◽  
pp. 140-149
Author(s):  
Ikenna Kingsley Uchendu ◽  
Henshaw Uchechi Okoroiwu
Keyword(s):  
Low Dose ◽  
Renal Toxicity ◽  
Histopathological Examination ◽  
Nigella Sativa ◽  
Intraperitoneal Administration ◽  
Albino Rats ◽  
High Dose ◽  
Protective Effects ◽  
Oral Group ◽  
Nigella Sativa Seed

Background: Prevalence of chemical-induced renal injuries has been on a fast rise over the years and has become the leading cause of mortality and morbidity in the society, with environmental pollutants, heavy metals inclusive, seen as the causal agents. Recently, the role of medicinal foods in human health has gained considerable attention. Objective: We investigated the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cadmium-induced renal toxicity in albino rats. Methods: Twenty-five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Renal injury was assessed by measuring serum levels of Na+, K+, creatinine and blood urea nitrogen (BUN) using standard methods. The kidneys were harvested for histopathological examination. Results: CdCl2 induced significant nephrotoxicity with marked elevation in the levels of biochemical markers of renal functions (p<0.05 or p<0.01); these were, however, ameliorated by a low dose of MENS. Histopathological examination of the kidney sections supported the biochemical findings. Conclusion: We conclude that Nigella sativa seed extract, at a low dose, is potentially nephroprotective against harmful chemical toxins such as cadmium.

scholarly journals Protection of histomorphology of vital organs by methanolic seed extract of Nigella sativa against cadmium-induced tissue injuries in rats

2021 ◽  
Vol 11 (3-S) ◽  
pp. 19-26
Author(s):  
Emeka Cyprian Oguji ◽  
Chibueze Joseph Obigeorge ◽  
Johnson Obiechina Omeh ◽  
Amechi Jnr. Odeku ◽  
Tachia Jaclyn Wanger ◽  
...  
Keyword(s):  
Nigella Sativa ◽  
Intraperitoneal Administration ◽  
Negative Control ◽  
Seed Extract ◽  
Albino Rats ◽  
High Dose ◽  
Protective Effects ◽  
Edible Plant ◽  
Plant Materials ◽  
Mortality And Morbidity

Background: Chemical-induced organ injuries have been on a fast rise for decades and these injuries have become common causes of mortality and morbidity in the society. Edible plant materials with medicinal properties have been used for treating various diseases for many centuries in folk medicine. Recently, the role of food or medicinal plants in human health has received considerable attention. Traditional uses of N. sativa seed range from soothing wounds to remedying cough, eczema, diabetes, inflammation of the bronchi and tooth aches; and these point to substantial tissue effects. Objective: We investigated the protective effects of methanolic seed extract of Nigella sativa (MENS) against cadmium-induced histomorphological alterations in heart, kidney and liver tissues of albino rats. Methods: Twenty five (25) male albino rats, weighing (200±20g), were randomly grouped into five groups: A, B, C, D, and E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), and group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 14 days. Group A (Normal control) received no administration. Heart, kidney and liver were harvested for histopathological analyses. Results: Cadmium (CdCl2) induced significant histomorphological changes in the studied organs, and the heart was the most damaged of all the organs studied; however a significantly ameliorative effect by methanolic seed extracts was observed. Conclusion: Nigella sativa seed extract is potentially tissue-protective against harmful chemical toxins like cadmium.  

Evaluation of blood oxidant/antioxidant changes and testicular toxicity after subacute exposure to cadmium in albino rats: Therapeutic effect of Nigella sativa seed extracts

2020 ◽  
Vol 23 ◽  
Author(s):  
Ikenna Kingsley Uchendu ◽  
Henshaw Uchechi Okoroiwu
Keyword(s):  
Oxidative Stress ◽  
Testosterone Level ◽  
Nigella Sativa ◽  
Serum Testosterone ◽  
Seed Extract ◽  
Serum Testosterone Level ◽  
Albino Rats ◽  
Testicular Toxicity ◽  
Oral Group ◽  
Testicular Injury

Aim and Objective: Cells and tissues of the body are prone to oxidative damage as a result of increased level of reactive oxygen species and nitrogen radical beyond the detoxifying ability of the endogenous antioxidant system. This study aimed to evaluate the ameliorative effect of methanolic extracts of Nigella sativa (MENS) against cadmium-induced blood oxidative stress and testicular toxicity in albino rats. Materials and Methods: Twenty five (25) male albino rats, weighing (200±20g), were randomly grouped into five groups (A-E). Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 14 days. No treatment was administered to group A (Normal control). The oxidative state of the blood was assessed by measuring the blood levels or activities of MDA, CAT, GSH and SOD; while testicular injury was assessed by measuring serum testosterone level using ELISA. The testes were harvested for histopathological examination. Results: The results showed that cadmium induced a marked elevation in the level of MDA, and a decrease in SOD, CAT and GSH levels or activities (p<0.05 or p<0.01); but no significant alteration in the serum testosterone level (p>0.05); Histopathological studies on the testes showed that cadmium significantly induced testicular injury, which was however ameliorated by the seed extract of N.sativa. Conclusion: We conclude that N.sativa seed extract is potentially testiculoprotective and attenuates oxidative stress against harmful chemical toxins such as cadmium.

scholarly journals Phytochemicals and protective effects of Moringa oleifera seed extract on CCl4- induced hepatotoxicity and hemotoxicity in rats

2018 ◽  
Vol 7 (12) ◽  
pp. 2286
Author(s):  
Eman S. S. Biomy ◽  
Mossad G. A. El-Sayed ◽  
Ashraf A. A. El-Komy
Keyword(s):  
Moringa Oleifera ◽  
Histopathological Examination ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Albumin Level ◽  
Seed Extract ◽  
Male Rats ◽  
High Dose ◽  
Protective Effects

Background: Moringa oleifera is high valued plant and used in many countries around the world. The seed of Moringa oleifera (MO) is an important part and has a remarkable medicinal, nutritional and socio-economic values, this study, therefore, was designed to clarify the protective effect of Moringa oleifera hydroethanolic seed extract (MOSE) against carbon tetrachloride (CCl4) induced hepatoxicity and hemotoxicity in rats.Methods: A total of one hundred and five male rats were randomly divided into 7 groups of 15 rats each. The hydroethanolic seed extract (30%) was administered orally for one month at 250 and 500mg/kg body weight. Samples were collected after day1,15 and 30 post administration.Results: Phytochemical, biochemical, hematological and hisopathological examinations were utilized to investigate hepatoprotective activity of MOSE. The results obtained demonstrated that, phytochemicals such as alkaloids, glycosides, anthraquinones, tannins, flavonoids, gum, resin, saponins, terponoids, protein and fats were detected in the seeds. Treatment with the MOSE caused a significant (P<0.05) decrease in the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, triglyceride and lipid peroxidation (MDA), while total protein and albumin level significantly (P<0.05) increased compared to CCl4 group. Also, treatment with the MOSE showed a significant (P<0.05) increase Hb content and RBCs, whereas WBCs and lymphocyte count significantly (P<0.05) decreased throughout the period of administration when compared to the rats in CCl4 group. The results obtained were comparable to silymarin. Histopathological examination of liver tissues confirmed the biochemical data.Conclusions: It could be concluded that, CCl4 induced hepatotoxicity and hemotoxicity is ameliorated by MOSE especially in high dose of (500mg/kg). This effect is attributed to free radical scavenging activity and potent antioxidant activity of its components (Flavonoid, tannin, alkaloid and saponin).

scholarly journals THERAPEUTIC EFFECTS OF HYDRO-ETHANOLIC EXTRACT OF Nigella sativa (BLACK SEED) ON SOME HAEMATOLOGICAL PARAMETERS OF ALBINO RATS AFTER LEAD EXPOSURE

2020 ◽  
Vol 14 (2) ◽  
pp. 131-135
Author(s):  
Abubakar A ◽  
◽  
Tukur MA ◽  
Ibrahim BM ◽  
Isa BI ◽  
...  
Keyword(s):  
Lead Poisoning ◽  
Nigella Sativa ◽  
Hematological Parameters ◽  
Seed Extract ◽  
Albino Rats ◽  
Therapeutic Effects ◽  
Hb Concentration ◽  
Nigella Sativa Seed ◽  
Haematological Indices ◽  
Rbc Count

Background: Lead poisoning is a great public health concern in Africa and Nigeria, especially regions where illegal mining activities occurs such as widespread gold ore mining in Zamfara by artisan miners using rudimentary and unsafe processing techniques. Lead (Pb) which often have no biological function remain in the system causes havoc and distortion of normal physiologic functions. Currently, the toxic effects of lead poisoning are clinically treated using chelation therapy which have been associated with many side effects and setbacks. Since ancient times, medicinal plants have been used as a major source of treatment for numerous human diseases. Plants that possess hematinic and antioxidant properties in the plant kingdom are often used in such scenarios. One of such plants is Nigella sativa commonly known as black cumin. Objectives: In this study, therapeutic effects of hydro-ethanolic extracts of Nigella sativa was assessed on hematological parameters of lead- poisoned albino rats as a curative therapy for management of lead poisoning. Method: Thirty- five (35) adult albino wistar rats of both sexes were used for this study. Group I (Normal control) received 2ml/kg of distilled water, Group II (Negative control), Group III treated with 10 mg/kg of Meso-2,3 Dimercaptosuccinic acid (DMSA), IV treated with 200 mg/kg of Nigella sativa seed extract, V treated with 400 mg/kg of Nigella sativa seed extract, and VI treated with 800mg/kg of Nigella sativa seed extract for Group VII (Recovery) were allowed to recover without treatment. The study lasted for a duration of 21days. Blood samples were collected from the rats through cardiac puncture after anesthetizing the animals and analysed for haematological parameters which included RBC count, PCV, Hb concentration, platelet count, WBC profile and haematological indices using an automated digital blood analyser. Results: Nigella sativa seed extract significantly (P<0.05) reversed the adverse effect of Lead exposure on RBC count, PCV, Hb concentration, platelet count, WBC profile and haematological indices. Conclusion: Nigella sativa showed therapeutic effects on hematological parameters and indices of lead poisoned albino rats.

scholarly journals Immunostimulatory potential of Nigella sativa seeds extract on chicken macrophage function

2021 ◽  
Vol 22 (3) ◽  
pp. 433-439
Author(s):  
Yogendra Singh ◽  
Patel Nikunjkumar ◽  
Beenam Saxena ◽  
Saravanan Ramakrisnan
Keyword(s):  
Mononuclear Cells ◽  
Nigella Sativa ◽  
Seed Extract ◽  
High Dose ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
Chicken Macrophage ◽  
Nigella Sativa Seed ◽  
Different Doses ◽  
Inos Gene Expression

In present study, the effect of Nigella sativa seeds extract on the chicken peripheral blood mononuclear cells (PBMCs) was investigated. The chicken PBMCs were stimulated with two different doses (125?g/ml; 250?g/ml) of Nigella sativa seeds extract and the cells were harvested at different time points till 48h post-stimulation for analysis of iNOS gene expression by quantitative PCR and nitric oxide (NO) estimation at 24 and 48h post-stimulation. High dose (250?g/ml) of Nigella sativa seed extract showed remarkable induction of iNOS transcripts expression and NO production at 48h post-stimulation, which were 12.8040±1.03347 folds and 5.7089±.64535µM respectively. The results indicated the immunostimulatory potential of the Nigella sativa seed extract on the chicken PBMCs.

Hepatotoxic effects of chloroform extract of Artemisia macivera Linn in rats following intraperitoneal administration of different subchronic doses

2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh
Keyword(s):  
Alkaline Phosphatase ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Intraperitoneal Administration ◽  
Chloroform Extract ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
High Dose ◽  
Hepatocellular Injury

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.

Protective Effects of Hypericum perforatum and Quercetin in a Rat Model of Ischemia/Reperfusion Injury of Testes

2017 ◽  
Vol 28 (01) ◽  
pp. 096-100
Author(s):  
Leyla Tekin ◽  
Mehmet Erdemli ◽  
Nazile Erturk ◽  
Zeynep Aksungur ◽  
Serdar Aktas ◽  
...  
Keyword(s):  
Antioxidant Status ◽  
Hypericum Perforatum ◽  
Ischemia Reperfusion Injury ◽  
Histopathological Examination ◽  
Ischemia Reperfusion ◽  
Total Antioxidant Status ◽  
Albino Rats ◽  
Protective Effects ◽  
Total Antioxidant ◽  
Markers Of Oxidative Stress

Purpose This study aimed to compare the protective effects of Hypericum perforatum (Hp) and quercetin, a flavonoid, against ischemia/reperfusion (I/R) injury in rat testes. Materials and Methods This study included 28 male Wistar albino rats that were divided into four groups. Except for the sham group, torsion was created by rotating both testes at an angle of 720 degrees clockwise for 2 hours. The Hp and quercetin groups received 25 mg/kg Hp and quercetin intraperitoneally 30 minutes before detorsion, respectively. Orchiectomy was performed for the measurement of markers of oxidative stress and histopathological examination. Results In the Hp and quercetin groups, malondialdehyde (MDA) and nitric oxide (NO) levels and total oxidant capacity were significantly lower, the glutathione level and total antioxidant status were significantly higher, and Johnsen's testis biopsy scores were significantly higher than in the torsion/detorsion group (p ˂ 0.001). The markers of oxidative injury were significantly lower (p ˂ 0.001) and total antioxidant status was significantly higher (p ˂ 0.001), except for glutathione (p = 0.62) in the Hp group than in the quercetin group. Johnsen's score between Hp and quercetin groups was not significantly different (p = 0.80). Conclusion Both Hp and quercetin have protective effects against I/R injury of the testes, but the protective effect of Hp was found to be stronger than that of quercetin.

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