scholarly journals Immunostimulatory potential of Nigella sativa seeds extract on chicken macrophage function

2021 ◽  
Vol 22 (3) ◽  
pp. 433-439
Author(s):  
Yogendra Singh ◽  
Patel Nikunjkumar ◽  
Beenam Saxena ◽  
Saravanan Ramakrisnan
Keyword(s):  
Mononuclear Cells ◽  
Nigella Sativa ◽  
Seed Extract ◽  
High Dose ◽  
No Production ◽  
Peripheral Blood Mononuclear ◽  
Chicken Macrophage ◽  
Nigella Sativa Seed ◽  
Different Doses ◽  
Inos Gene Expression

In present study, the effect of Nigella sativa seeds extract on the chicken peripheral blood mononuclear cells (PBMCs) was investigated. The chicken PBMCs were stimulated with two different doses (125?g/ml; 250?g/ml) of Nigella sativa seeds extract and the cells were harvested at different time points till 48h post-stimulation for analysis of iNOS gene expression by quantitative PCR and nitric oxide (NO) estimation at 24 and 48h post-stimulation. High dose (250?g/ml) of Nigella sativa seed extract showed remarkable induction of iNOS transcripts expression and NO production at 48h post-stimulation, which were 12.8040±1.03347 folds and 5.7089±.64535µM respectively. The results indicated the immunostimulatory potential of the Nigella sativa seed extract on the chicken PBMCs.

scholarly journals Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats

2020 ◽  
Vol 10 (1-s) ◽  
pp. 174-177
Author(s):  
Ikenna Kingsley Uchendu ◽  
Ebuka Bitrus Nnedu ◽  
Ifeoma Blessing Ekeigwe
Keyword(s):  
Histopathological Examination ◽  
Nigella Sativa ◽  
Intraperitoneal Administration ◽  
Negative Control ◽  
Seed Extract ◽  
Albino Rats ◽  
High Dose ◽  
Protective Effects ◽  
Oral Group ◽  
Nigella Sativa Seed

Objective:  The aim of this study is to investigate the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cardiotoxicity of cadmium in albino rats.  Methods: Twenty five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Cardiac injury was assessed by measuring serum levels of Aspartate aminotransferase (AST), Lactate dehydrogenase (LDH) and Creatine kinase (CK-MB) using standard methods. The heart was harvested for histopathological examination. Results: CdCl2 induced significant cardiotoxicity with marked elevation in the levels of biochemical markers of cardiac functions (p<0.05 or p<0.01); these were however attenuated by MENS. Histopathological examination of the heart sections supported the biochemical findings. Conclusion: Nigella sativa seed extract is potentially cardioprotective against harmful chemical toxins such as cadmium. Keywords: Cadmium, CdCl2, Nigella sativa, Medicinal food, cardiotoxicity

scholarly journals Comparison of Antibacterial Efficacy of Fenugreek Seed Extract Rinse and Nigella Sativa Seed Extract Rinse against Streptococcus Mutant Colonies

2021 ◽  
pp. 79-86
Author(s):  
Nasima Iqbal ◽  
ATA UR Rehman ◽  
Syeda Amber Zaidi ◽  
Kiran Khan ◽  
Lubna Farooq ◽  
...  
Keyword(s):  
Nigella Sativa ◽  
Positive Control ◽  
Seed Extract ◽  
Control Group ◽  
Distilled Water ◽  
Post Hoc Analysis ◽  
Fenugreek Seed ◽  
Study Participants ◽  
Post Hoc ◽  
Nigella Sativa Seed

Background: Dental infections were caused by the bacterium overgrowth on the surface of the tooth, and treatment should always be set up to prevent this development.Antibiotics have long been used as a conventional antibacterial medication, but their overuse has resulted in microbes gaining resistance to many of the antibiotics, trying to make many commercialized therapeutic remedies ineffectual and resulting to infection recurrence. In this regard we aim to analyze the antibacterial activity of nigella sativa seeds’ and fenugreek seed extract rinses against S. mutans' colonies. Methodology: It was a preclinical experimental study conducted at Baqai Medical and Dental College Karachi., from January - June 2021. Calculated sample size was n = 80 Consecutive sampling technique was used. Plaque of study participants was collected on sterile strips that was transported to laboratory for culture in sterile containers.The extract of Fenugreek seed and nigella sativa seed was kept in an airtight bottle and stored in a refrigerator till usage. The extracts were diluted in distilled water in 1:4 (Extract: Distilled water). Study participants were instructed to not brush their teeth before sampling. Study participants were divided into four groups (negative control, positive control, fenugreek seed extract group and Nigella sativa group) each group had 20 participants. Diluted Fenugreek seed extract and Nigella sativa extract was given to experimental groups for rinses. Results: There was significant decrease in number of colonies in positive controls, fenugreek seed extract group and nigella sativa extract group and there was no change in number of colonies in control group. Furthermore, the analysis showed significant (p-value = 0.001) difference among the groups followed by post hoc analysis. Post hoc analysis showed no difference between positive control, fenugreek seed extract group and nigella sativa group. Conclusion: Fenugreek seed extract and Nigella Sativa seed extract showed comparable antibacterial properties. Also, the effect was found to be similar to commercially available mouth rinse.

scholarly journals Measles Virus Spread and Pathogenesis in Genetically Modified Mice

1998 ◽  
Vol 72 (9) ◽  
pp. 7420-7427 ◽  
Author(s):  
Branka Mrkic ◽  
Jovan Pavlovic ◽  
Thomas Rülicke ◽  
Pietro Volpe ◽  
Christian J. Buchholz ◽  
...  
Keyword(s):  
Measles Virus ◽  
Mononuclear Cells ◽  
High Dose ◽  
Peripheral Blood Mononuclear ◽  
High Affinity ◽  
Beta Interferon ◽  
Targeted Mutation ◽  
High Affinity Receptor ◽  
Affinity Receptor ◽  
Alpha Beta

Attenuated Edmonston measles virus (MV-Edm) is not pathogenic in standard mice. We show here that MV-Edm inoculated via the natural respiratory route has a limited propagation in the lungs of mice with a targeted mutation inactivating the alpha/beta interferon receptor. A high dose of MV-Edm administered intracerebrally is lethal for about half of these mice. To study the consequences of the availability of a high-affinity receptor for MV propagation, we generated alpha/beta interferon-defective mice expressing human CD46 with human-like tissue specificity. Intranasal infection of these mice with MV-Edm resulted in enhanced spread to the lungs and more prominent inflammatory response. Virus replication was also detected in peripheral blood mononuclear cells, the spleen, and the liver. Moreover, intracerebral inoculation of adult animals with low MV-Edm doses caused encephalitis with almost inevitably lethal outcome. We conclude that in mice alpha/beta interferon controls MV infection and that a high-affinity receptor facilitates, but is not strictly required for, MV spread and pathogenesis.

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