Allelopathic effect of leaf extracts of Punica granatum and Spiraea prunifolia against post-harvest rot of tomato and brinjal

Antimycotic activities of different solvent extracts of Punica granatum L. and Spiraea prunifolia Siebold and Zucc. were carried out through agar well diffusion assay at three concentrations (25µl, 50µl and 75µl) against seven fungi causing rot diseases of tomato and brinjal. All the concentration of plant extracts showed antifungal activity against tested pathogenic fungi. Antimycotic activity increased with the increased concentrations of plant extracts. However, higher concentrations proved more effective than lower concentrations. It was revealed from the present study that the ethanolic extract of Punica granatum L. showed maximum antifungal activity against Rhizoctonia solani and Penicillium expansum and least inhibitory activity against Aspergillus niger. However, the aqueous extract of Punica granatum L. showed maximum antimycotic activity against Rhizoctonia solani and Alternaria alternata and least inhibitory effect against Penicillium expansum. It was further observed from the present study that the ethanolic extract of Spiraea prunifolia Siebold and Zucc. showed maximum antimycotic activity against Rhizoctonia solani and least inhibitory effect against Alternaria alternata. Whereas the aqueous extract of Spiraea prunifolia Siebold and Zucc showed maximum antifungal activity against Aspergillus niger and least inhibitory activity against Alternaria alternata. Keywords: Plant extracts, Concentration, Antimycotic effectiveness, tomato and brinjal, fungal rot pathogens, Inhibition zone.

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Evaluation of different plant extracts for effective management of fungal rot of tomato and brinjal in Kashmir Valley

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs(2020)071610 ◽

2020 ◽

Vol 7 (16) ◽

pp. 229-238

Author(s):

Jahangir Abdullah Koka ◽

Abdul Hamid Wani ◽

Mohd Yaqub Bhat

Keyword(s):

Rhizoctonia Solani ◽

Plant Extracts ◽

Ethanolic Extract ◽

Pathogenic Fungi ◽

Inhibitory Effect ◽

Penicillium Expansum ◽

Paeonia Suffruticosa ◽

Prunella Vulgaris ◽

Antimycotic Activity

The aim of present research was focused on the antifungal activities of Prunella vulgaris L and Paeonia suffruticosa Andrews via in vitro approach through agar well diffusion assay at three concentrations (25 uL, 50 uL and 75 uL) against fungi causing diseases in tomato and brinjal. All the concentration of plant extracts showed antimycotic activity against tested pathogenic fungi. Antimycotic activity increased with the increased concentrations of plant extracts. However, higher concentrations proved more effective than lower concentrations. It was revealed from the present study that the ethanolic and aqueous extracts of Prunella vulgaris L showed maximum antimycotic activity against Rhizoctonia solani and least inhibitory effect against Penicillium chrysogenum. It was further revealed from the present study that the ethanolic extract of Paeonia suffruticosa Andrews showed maximum antimycotic activity against Penicillium expansum and least activity against Mucor plumbeus. Whereas the aqueous extract of Paeonia suffruticosa Andrews showed maximum antimycotic activity against Rhizoctonia solani and Penicllium expansum and least inhibitory effect against Mucor plumbeus.

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Antimycotic activity of ethanolic and aqueous leaf extracts of Ajuga bracteosa Wall. ex Benth. (Lamiale: Lamiaceae) and Iris kashmiriana Baker (Asparagales: Iridaceae) against some vegetable rot fungi

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.050908 ◽

2018 ◽

Vol 5 (9) ◽

pp. 75-84

Author(s):

Jahangir Abdullah Koka ◽

Abdul Hamid Wani ◽

Mohd Yaqub Bhat ◽

Tariq Ahmad Wani ◽

Shazia Parveen

Keyword(s):

Aspergillus Niger ◽

Rhizoctonia Solani ◽

Aqueous Extract ◽

Plant Extracts ◽

Penicillium Chrysogenum ◽

Ethanolic Extract ◽

Penicillium Expansum ◽

Leaf Extracts ◽

Trichothecium Roseum ◽

Antimycotic Activity

Antifungal activities of different solvent extracts of Ajuga bracteosa Wall. ex Benth. (Lamiale: Lamiaceae) and Iris kashmiriana Baker (Asparagales: Iridaceae) were carried out through agar well diffusion assay at three concentrations (25 uL, 50 uL and 75 uL) against seven rot causing fungi, viz. Penicillium expansum, Aspergillus niger, Mucor plumbeus, Alternaria alternata, Penicillium chrysogenum, Trichothecium roseum and Rhizoctonia solani. All the concentration of plant extracts showed antimycotic activity against tested pathogenic fungi. Antimycotic activity increased with the increased concentrations of plant extracts. However, higher concentrations proved more effective than lower concentrations. It was revealed from the present study that the ethanolic extract of Ajuga bracteosa showed maximum antimycotic activity against Mucor plumbeus and Rhizoctonia solani and least activity against Penicillium chrysogenum. However, the aqueous extract of Ajuga bracteosa showed maximum antifungal activity against Rhizoctonia solani and Penicillium expansum and least activity against Trichothecium roseum. It was further revealed from the present study that the ethanolic extract of Iris kashmiriana showed maximum antimycotic activity against Aspergillus niger and least activity against Rhizoctonia solani. Whereas the aqueous extract of Iris kashmiriana showed maximum antimycotic activity against Penicillium expansum and Rhizoctonia solani and least activity against Aspergillus niger.

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Antifungal activity of some botanical extracts on Fusarium oxysporum

Open Life Sciences ◽

10.1515/biol-2015-0040 ◽

2015 ◽

Vol 10 (1) ◽

Cited By ~ 5

Author(s):

Domenico Rongai ◽

Patrizio Pulcini ◽

Barbara Pesce ◽

Filomena Milano

Keyword(s):

Antifungal Activity ◽

Growth Inhibition ◽

Fusarium Oxysporum ◽

Plant Extracts ◽

Mycelial Growth ◽

Titratable Acidity ◽

Punica Granatum ◽

Inhibitory Effect ◽

Total Phenolic ◽

Conidia Germination

AbstractThe present paper describes the antifungal activity of some plant extracts on the development of Fusarium oxysporum f.sp. lycopersici. The best extracts were selected to be tested as a phytofungicide to control crop diseases, with the ultimate goal of developing a green alternative to synthetic fungicides. Using the conidia germination assay, of the 24 plant extracts tested, 15 reduced conidia germination and 6 completely inhibited germination. Extracts of Rivina humulis, Brassica carinata, Brunfelsia calyicina, Salvia guaranitica and Punica granatum showed good antifungal activity. The relationship between total phenolic content (TPC) in each plant extract tested and the percentage of mycelial growth inhibition showed a significant correlation (R2 = 0.69), while no correlation was found between total flavonoid content (TFC) and percentage mycelial growth inhibition. Among all extracts tested, Punica granatum and Salvia guaranitica showed the best inhibitory effect against Fusarium oxysporum f.sp. lycopersici . Our results indicate that plant extracts with a good antifungal activity generally had a high level of total polyphenolic content and titratable acidity, and low values of pH.

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In vitro antifungal activity of three Saudi plant extracts against some Phytopathogenic fungi

Journal of Plant Protection Research ◽

10.2478/v10045-012-0073-9 ◽

2012 ◽

Vol 52 (4) ◽

pp. 458-462 ◽

Cited By ~ 12

Author(s):

Abdul Aziz A. Al-Askar

Keyword(s):

Antifungal Activity ◽

Plant Extracts ◽

Sclerotium Rolfsii ◽

Ethanolic Extract ◽

Punica Granatum ◽

Phytopathogenic Fungi ◽

Plant Diseases ◽

High Concentration ◽

In Vitro Antifungal Activity

Abstract The antifungal activities of ethanolic extracts of three Saudi plants; camel thorn (Alhagi maurorum Medic.), caper (Capparisspinosa L.), and pomegranate (Punica granatum L.) were investigated in vitro against Alternaria alternata, Fusarium oxysporum, Phomadestructiva, Rhizoctonia solani, and Sclerotium rolfsii at concentrations of 0, 3, 6, and 9% (v/v). All tested plant extracts; seeds, roots, and rinds had different degrees of antifungal activity against the tested fungi. When compared with the control, the highest antifungal activity was recorded for camel thorn seeds extract at a concentration of 9%, while, pomegranate rinds extract at 9% came in second. Camel thorn rinds extract came in last even when used at a high concentration. The ethanolic extract of camel thorn seeds may be recommended as a potent bio-fungicide. Extensive studies should be undertaken for the ethanolic extract of camel thorn seeds as a strong antifungal agent against fungal plant diseases.

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The Effect of Aqueous and Alcoholic Extracts of Punica granatum L. Pericarp on Hemolysin Production of several Bacterial species

Baghdad Science Journal ◽

10.21123/bsj.7.1.309-316 ◽

2010 ◽

Vol 7 (1) ◽

pp. 309-316

Author(s):

Baghdad Science Journal

Keyword(s):

Inhibitory Concentration ◽

Bacterial Species ◽

Ethanolic Extract ◽

Punica Granatum ◽

Inhibitory Effect ◽

Gram Negative Bacteria ◽

Bacterial Isolates ◽

E Coli ◽

Gram Positive Bacteria ◽

Punica Granatum L

Four local hemolysin producer bacterial isolates were selected, tow of them gram negative bacteria (Escherichia coli ,Pseudomonas aeruginosa ) and the other two were gram positive bacteria (Staphylococcus aureus , Bacillus cereus ). Minimum inhibitory concentration of the aqueous and alcoholic extracts of Punica granatum L. pericarp were determined towards the four bacterial isolates ,results obtaind showed that MICs of the aqueous extract were 200 mg/ml for E .coli and P. aeruginosa isolates while were 5 mg/ml and 2 mg/ml for B. cereus, S. aureus , respectively The MICs for the ethanolic extract were 50 mg/ml , 20 mg/ml ,1 mg/ml ,0.5 mg/ml for E. coli ,P. aeruginosa ,B. cereus ,S. aureus , respectively. The effect of Sub-MICs of the aqueous and alcoholic extracts on hemolysin production was investigated , both extracts had a suppressing effect on hemolysin production by E. coli ,P. aeruginosa ,while both extract had an inhibitory effect on hemolysin production by S. aureus and B . cereus isolates

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Inhibitory Effect of thePunica granatumFruit Extract on Angiotensin-II Type I Receptor and Thromboxane B2 in Endothelial Cells Induced by Plasma from Preeclamptic Patients

Advances in Preventive Medicine ◽

10.1155/2016/6028989 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Widya Kusumawati ◽

Kusnarman Keman ◽

Setyawati Soeharto

Keyword(s):

Endothelial Cells ◽

Angiotensin Ii ◽

Vascular Endothelial Cells ◽

Ethanolic Extract ◽

Punica Granatum ◽

Normal Pregnancy ◽

Inhibitory Effect ◽

Thromboxane B2 ◽

High Dose ◽

Type I

This study aims to evaluate whether thePunica granatumfruit extract modulates the Angiotensin-II Type I receptor (AT1-R) and thromboxane B2 level in endothelial cells induced by plasma from preeclamptic patients. Endothelial cells were obtained from human umbilical vascular endothelial cells. At confluence, endothelial cells were divided into five groups, which included endothelial cells exposed to 2% plasma from normal pregnancy (NP), endothelial cells exposed to 2% plasma from preeclamptic patients (PP), and endothelial cells exposed to PP in the presence of ethanolic extract ofPunica granatum(PP + PG) at the following three doses: 14; 28; and 56 ppm. The expression of AT1-R was observed by immunohistochemistry technique, and thromboxane B2 level was done by immunoassay technique. Plasma from PP significantly increased AT1-R expression and thromboxane B2 levels compared to cells treated by normal pregnancy plasma. The increasing of AT1-R expression significantly (P<0.05) attenuated by high dose treatments ofPunica granatumextract. Moreover, the increasing of thromboxane B2 levels significantly (P<0.05) attenuated by lowest dose treatments ofPunica granatumextract. We further concluded thatPunica granatumfruit protects and inhibits the sensitivity of endothelial cells to plasma from preeclamptic patients due to inhibition of AT1-R expression (56 ppm) and reduced thromboxane B2 levels (14 ppm).

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PHYTOCHEMICAL EVALUATION OF PUNICA GRANATUM L. LEAF EXTRACT

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i4.1159 ◽

2017 ◽

Vol 9 (4) ◽

pp. 14 ◽

Cited By ~ 2

Author(s):

P. Sreedevi ◽

K. Vijayalakshmi ◽

R. Venkateswari

Keyword(s):

Metabolic Diseases ◽

Ethanolic Extract ◽

Punica Granatum ◽

Leaf Extracts ◽

Bioactive Substances ◽

Pharmaceutical Ingredients ◽

Wide Range ◽

Leaf Powder ◽

Potent Drug ◽

Punica Granatum L

Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.

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