scholarly journals Administration of Thymoquinone Offer a Protective Effect through the Apoptogenic and Antioxidant Pathway in Acute Liver Failure induced by Taxol

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Mohammad Reza Salahshoor ◽  
Shiva Roshankhah ◽  
Cyrus Jalili ◽  
Amir Farmandeh
Keyword(s):  
Normal Control Group ◽  
Apoptotic Index ◽  
Male Rats ◽  
Control Group ◽  
Hepatic Enzymes ◽  
Control Groups ◽  
Nitrite Oxide ◽  
Relative Expression Level ◽  
Total Antioxidant ◽  
Apoptotic Genes

Objectives Due to the increasing use of chemotherapy drug and herbs in medicine, this study was designed to assess the effects of Thymoquinone and Taxol on apoptogenic, oxidative and histomorphometric changes in liver. Methods Sixty-four male rats were assigned to 8 groups including control groups (normal group and Taxol (20 mg/kg group), Thymoquinone groups (4.5, 9, 18 mg/kg) and Taxol + Thymoquinone (TT) treated groups. All experimental groups were treated intraperitoneally daily for two weeks. The relative expression level of apoptotic genes and hepatocyte apoptotic index were analyses. Also, Nitrite oxide (NO), lipid peroxidation (LP), total antioxidant capacity (TAC), hepatic enzymes and histomorphometric parameters were evaluated.  Results In the Taxol control group (TCG) all parameters investigated in this study significantly increase except TAC level, which was decreased in compared to the normal control group (NCG) (P < 0.01). Additionally, all evaluated parameters were reduced in Thymoquinone and TT groups, except TAC level (which was increased) as compared to the TCG (P < 0.01). Conclusion Our results discovered that Thymoquinone successfully moderate liver injury induced by Taxol through the activation of antioxidant pathways, reduce the apoptogenic and the regeneration of histopathological alterations.

scholarly journals Toxicological evaluation of the nicotine on CA1 and protective effects of curcuma longa: An experimental study

2019 ◽  
Vol 6 (9) ◽  
pp. 3368-3378
Author(s):  
Mohammad Reza Salahshoor ◽  
Shiva Roshankhah ◽  
Babak Arji Roudsari ◽  
Cyrus Jalili
Keyword(s):  
Antioxidant Capacity ◽  
Dendritic Spines ◽  
Total Antioxidant Capacity ◽  
Curcuma Longa ◽  
Male Rats ◽  
Control Group ◽  
Therapeutic Effects ◽  
Nitrite Oxide ◽  
Ca1 Region ◽  
Total Antioxidant

Introduction: Nicotine is the most important alkaloid compound in tobacco and is a major risk factor in the development of functional disorders of several organ systems. Some plants produce Curcuma longa (curcumin), which has antioxidant and neuro-protective properties. Methods: This study was designed to evaluate the therapeutic effects of curcumin against nicotine injury on the hippocampus CA1 region of rats. In this study, 48 male rats were randomly assigned to eight groups: Normal control (saline) group, Nicotine control group (0.5 mg/kg); Curcumin groups (10, 30, and 60 mg/kg) and Nicotine + Curcumin groups (10, 30, and 60 mg/kg). Treatments were administered intra-peritoneally daily for 28 days. Golgi staining technique investigated the number of dendritic spines. Cresyl violet staining method was used to determine the number of neurons in the hippocampal region CA1. Griess technique was assessed to determine serum nitrite oxide levels. Also, the ferric reducing/antioxidant power (FRAP) method was applied to determine the total antioxidant capacity. Results: Nicotine administration significantly increased the nitrite oxide level and decreased total antioxidant capacity. The number of neuronal dendritic spines, as well as neurons per se, also decreased, compared to the control group (p < 0.01). In all the members of the Curcumin and Nicotine + Curcumin groups, the number of neurons, neuronal dendritic spines and total antioxidant capacity increased significantly compared to the nicotine control group, while nitrite oxide level decreased significantly compared to the nicotine control group (p < 0.01). Conclusions: It seems that Curcumin administration improved hippocampal CA1 region injury in rats caused by Nicotine.  

scholarly journals Resveratrol Attenuates Malathion Induced Damage in Some Reproductive Parameters by Decreasing Oxidative Stress and Lipid Peroxidation in Male Rats

2019 ◽  
Author(s):  
Cyrus Jalili ◽  
Shiva Roshankhah ◽  
Mohammad Reza Salahshoor ◽  
Mohammad Mehdi Mohammadi
Keyword(s):  
Normal Control ◽  
Normal Control Group ◽  
Male Rats ◽  
Control Group ◽  
Reproductive Parameters ◽  
Reproductive Parameter ◽  
Short Term ◽  
Total Antioxidant ◽  
Term Administration ◽  
Antioxidant Effects

Objective: Malathion is the most organophosphates which capable to produce free radicals and induce disturbance on some of male reproductive parameter. Resveratrol is an herbal polyphenol and it has been beneficial antioxidant effects during short-term administration. This study was designed to evaluate the effects of Resveratrol against damage induced by Malathion to the reproductive parameter of male rats. Materials and methods: In this experimental study, 48 male rats were randomly assigned to 8 groups: normal control (saline) and Malathion control (250 mg/kg) groups; Resveratrol groups (2, 8, 20 mg/kg) and Malathion + Resveratrol (2, 8, 20 mg/kg). Treatments were administered intraperitoneally and gavage daily for 65 days. The sperm parameters, testis malondialdehyde (MDA), total antioxidant capacity (TAC), testosterone level and germinal layer height were evaluated and statistically analyzed. Results: The results displayed that the values of all parameters except MDA level (which increased) reduced significantly in the Malathion control group compared to the normal control group (p < 0.001). The Resveratrol and Resveratrol + Malathion treatments at all doses increased significantly all parameters except MDA level (which decreased) compared to the Malathion control group (p < 0.001). No significant modifications were observed in all Resveratrol groups compared to the normal control group (p > 0.05). Conclusion: Resveratrol attenuates toxic effect of Malathion on some of male reproductive parameters.

The potential protective effect of α-lipoic acid against nanocopper particle–induced hepatotoxicity in male rats

2016 ◽  
Vol 36 (9) ◽  
pp. 881-891 ◽  
Author(s):  
AA Khalaf ◽  
AR Zaki ◽  
MK Galal ◽  
HA Ogaly ◽  
MA Ibrahim ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Copper Content ◽  
Experimental Period ◽  
Liver Function Tests ◽  
Male Rats ◽  
Control Group ◽  
Histopathological Analysis ◽  
Tissue Samples ◽  
Group I ◽  
Apoptotic Genes

The present research task is aimed to evaluate the role of exogenous α-lipoic acid (ALA) (100 mg/kg body weight) as hepatoprotective and potent antioxidant in amelioration of copper nanoparticle (CNP)-induced hepatotoxicity. Forty male rats were randomly assigned into four equal groups: group I (control), group II received CNPs, group III received CNPs + ALA, and finally group IV received ALA for 2 months. At the end of the experimental period, the rats were decapitated, and blood and liver tissue samples were collected for measurement of liver function tests, antioxidant status, lipid peroxidation (LPO), copper content, expression of some apoptotic genes, and histopathological analysis. CNPs induced marked hepatic damages as evident by severe alteration in hepatic biomarkers. This was accompanied by a significant elevation in hepatic LPO and induced nitric oxide, copper content, and expression level of apoptotic genes (C-myc and C-jun). In contrast, marked depletion for antioxidant parameters was detected. These findings were confirmed with severe pathological alterations. Coadministration of ALA as a powerful antioxidant attenuates the hepatotoxic effects of CNPs through improvement of liver parameters, oxidative status, genetic changes, and preservation of liver integrity through histopathological analysis. These results suggest that consumed ALA could be used as an applicable hepatoprotective agent against oxidative damage mediated by nanoparticles intoxication.

scholarly journals IMPACTS OF QUERCETIN AND MERCAPTOTHION ON MALE REPRODUCTIVE PARAMETERS OF RATS

SPERMOVA ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 64-73
Author(s):  
Shiva Roshankhah ◽  
◽  
Ahmad Shabanizadeh ◽  
Amir Abdolmaleki ◽  
Mohammad Reza Salahshoor1 ◽  
...  
Keyword(s):  
Male Fertility ◽  
Caspase 3 ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Control Group ◽  
Germinal Layer ◽  
Treatment Groups ◽  
Real Time Quantitative Pcr ◽  
Total Antioxidant ◽  
Fertility Disorders

Quercetin is an herbal polyphenol with valuable antioxidant properties. Mercaptothion is categorized as organophosphates which can generate free radicals and induce male fertility disorders. This study was aimed to assess the impacts of Quercetin against destruction of male fertility parameters induced by Mercaptothion. 64 male rats were randomly assigned into 8 groups; control, and Mercaptothion (250 mg/kg) groups; Quercetin groups (7.5, 15, and 30 mg/kg) and Mercaptothion + Quercetin (7.5, 15, and 30 mg/kg). Treatments were administered intraperitoneally (Mercaptothion) and orally (Quercetin) daily for 65 days. The sperm parameters, testis malondialdehyde (MDA), total antioxidant capacity (TAC), testosterone level and height of germinal layer were evaluated. Expressions of p53, caspase-3, Bax, and Bcl-2 were measured through real-time quantitative PCR. Values of all factors were reduced significantly except the MDA level (which increased) in Mercaptothion group compared to the control group (p<0.001). Studied criteria in groups of Quercetin and Quercetin + Mercaptothion in whole doses increased significantly except MDA level (which reduced) compared to the Mercaptothion group (p<0.001). Also, downregulated levels of p53, caspase-3, and Bax genes and unregulated levels of Bcl-2 gene expression were detected in control and the sixth treatment groups significantly in Quercetin group compared to the Mercaptothion group (p<0.001). No significant alterations were detected in Quercetin groups compared to the control group (p>0.05). Quercetin reduced toxic effects of Mercaptothion on male fertility parameters.

scholarly journals Protective Role of Ce Nanoparticles Against the Hepatotoxicity Induced by Exposure to Paraquat

2018 ◽  
Vol 6 (2) ◽  
pp. 37-43
Author(s):  
Hamid Heidary Dartoti ◽  
Farzin Firozian ◽  
Sara Soleimani Asl ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Role ◽  
Male Rats ◽  
Hepatic Tissue ◽  
Control Group ◽  
Serum Samples ◽  
Tissue Samples ◽  
Total Antioxidant ◽  
Group 2

Objectives: The present study aimed to investigate the antioxidant activity of cerium oxide nanoparticles (CeNPs) against paraquat (PQ)-induced liver injury in rats. Methods: Thirty-two male rats were divided into four 8-member groups and treated intraperitoneally with PQ and/or CeNPs for 14 days. Group 1 received PQ (5 mg/kg/d), group 2 received CeNPs (15, 30, and 60 mg/kg/d), group 3 received a combination of PQ (5 mg/kg/d) and CeNPs (15, 30, and 60 mg/kg/d), and group 4 (control group) received saline solution. Serum samples along with liver tissue samples were collected from all the rats. Oxidative stress (OS) biomarkers including total antioxidant capacity, lipid peroxidation, total thiol groups, DNA damage, and nitric oxide levels were determined. Histological samples were also analyzed using hematoxylin and eosin staining slides. Results: Levels of oxidative stress and hepatic tissue damage were significantly higher in the PQ group compared to the control group. CeNPs at a dose of 15 mg/kg showed the antioxidant activity and compromised the PQ-induced damage. Conclusion: In the scenario tested in this study, CeNPs could reduce the levels of OS, as well as hepatic damage induced by PQ.

scholarly journals Effects of Cultured Cordycep militaris on Sexual Performance and Erectile Function in Streptozotocin-Induced Diabetic Male Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Sureena Pohsa ◽  
Wanthanee Hanchang ◽  
Nattapong Singpoonga ◽  
Peerasak Chaiprasart ◽  
Pornnarin Taepavarapruk
Keyword(s):  
Normal Control ◽  
Erectile Function ◽  
Sperm Count ◽  
Biological Activities ◽  
Mating Behaviour ◽  
Diabetic Rats ◽  
Normal Control Group ◽  
Male Rats ◽  
Control Group ◽  
Silkworm Pupae

Cordyceps militaris (CM), a valuable edible and medicinal fungus, has been used as traditional medicine to treat health conditions, as well as hyposexuality in Asian societies for over a century. Due to the high demand, several artificial cultivation methods have been developed for their biological activities. In this study, CM was cultured on medium that contained white rice and silkworm pupae, and the levels of cordycepin and adenosine, as well as its aphrodisiac effects in diabetes-induced erectile dysfunction (DIED), were evaluated. Diabetic rats were induced by streptozotocin (STZ) injection and administered orally with CM (0.1, 0.5, and 1.0 g/kg BW/day) for 3 weeks. Diabetic rats in negative and positive control groups received vehicle and sildenafil citrate (5 mg/kg), respectively. Results showed the changes in mating behaviour in which mount latency and intromission latency were significantly increased in diabetic rats, compared with the normal control group. Diabetic rats also showed a significant reduction in intracavernosal pressure (ICP) response to cavernous nerve stimulation, sperm count, testosterone level, penile nitric oxide synthase (NOS), and testicular superoxide dismutase (SOD) activities, when compared to the normal control group. Administration of CM (0.1, 0.5, and 1.0 g/kg BW/day) reversed the effects of diabetes on the mating behaviour, and the ICP responses to electrical stimulation. Moreover, the levels of penile NOS, testicular SOD activities, testosterone, and sperm count were significantly increased, and testicular malondialdehyde (MDA) levels were significantly decreased in these treated diabetic rats. Diabetic rats treated with sildenafil showed a significant induction in intromission frequency and NOS and SOD activities, as well as a marked increase in ICP responses. These results suggest that CCM exerts its aphrodisiac effect, possibly through activating testosterone production and suppressing oxidative stress to enhance erectile function in diabetic rats.

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ni Putu Ermi Hikmawanti ◽  
Lusi Putri Dwita ◽  
Dimas W. Wisnunanda ◽  
Fanny Farista
Keyword(s):  
Antioxidant Activity ◽  
Normal Control ◽  
Antioxidant Activities ◽  
Negative Control Group ◽  
Negative Control ◽  
Pharmaceutical Product ◽  
Normal Control Group ◽  
Male Rats ◽  
Control Group ◽  
Hydroethanolic Extract

Abstract Aim evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats. Methods Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test. Results The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05). Conclusion The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

Experimental Observation on Antiatheroscloresis Ability of Total Flavonoids from Jumi in Hyperlipidemia Rats

2011 ◽  
Vol 140 ◽  
pp. 137-141
Author(s):  
Guang Tao Xu ◽  
Xiao Wei Wu ◽  
Xin Mei Zhou ◽  
Shi Piao Chen ◽  
Shen Rong Shen
Keyword(s):  
Dose Group ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Normal Control Group ◽  
Atherogenic Index ◽  
Control Group ◽  
Total Flavonoids ◽  
Low Doses ◽  
Control Groups

In this study, we observed antiatheroscloresis ability of food-roune hyperlipidemia by total falconoid from Jumi in rats. We used hyperlipidemia model of rats established, intervened by total falconoid from Jumi. Rats were divided into normal control group, hyperlipidemia control group, higher dose group and lower dose group to observe the level of total cholesterol (TC), trilaurate glycerin (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and atherogenic index (AI), and take rat liver for regular pathological dyeing observation. The results shown that the total falconoid from Jumi could reduce the serum levels of TC, TG, LDL and AI significantly, and increase HDL/TC in compared with hyperlipidemia control groups. It had significantly curative to effect hepatic adipose infiltration in rats, especially in low doses of flavonoid. Total flavonoids from Jumi can regulate disturbance of lipid metabolism, hepatic adipose infiltration and arteriosclerosis.

scholarly journals Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

2016 ◽  
Vol 66 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Marija Stojanović ◽  
Ljiljana Šćepanović ◽  
Olivera Bosnić ◽  
Dušan Mitrović ◽  
Olga Jozanov-Stankov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Defense Mechanism ◽  
Total Antioxidant Status ◽  
Male Rats ◽  
Control Group ◽  
Acute Administration ◽  
Rat Intestine ◽  
Homocysteine Thiolactone ◽  
Total Antioxidant

AbstractOxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.

scholarly journals The protective effect of Pentoxifylline on testopathy in male rats following Dimethyl Nitrosamine administration: An experimental study

2019 ◽  
Author(s):  
Mohammad Reza Salahshoor ◽  
Azita Faramarzi ◽  
Shiva Roshankhah ◽  
Cyrus Jalili
Keyword(s):  
Experimental Study ◽  
Normal Control ◽  
Normal Control Group ◽  
Male Rats ◽  
Inflammatory Factors ◽  
Control Group ◽  
Peripheral Blood Flow ◽  
Reproductive Parameters ◽  
Carcinogenic Agent ◽  
Xanthine Derivative

Background: Nitrosamines as a carcinogenic agent has unfavorable effects on some of the male reproductive parameters. Pentoxifylline (PX) is a xanthine derivative used as a drug inhibiting the inflammatory factors, reducing blood viscosity, improving peripheral blood flow, and so on. Objective: The aim of the present study is to evaluate the effects of PX against Dimethyl nitrosamine (DMN)-inducing the damage to the reproductive parameter of male rats. Materials and Methods: In this experimental study, 48 male Wistar rats (8 wk, 220- 250 gr) were randomly assigned to eight groups (n = 6/each): normal control and DMN control groups (40 mg/kg); PX groups (25, 50, 100 mg/kg), and DMN + PX groups (25, 50, 100 mg/kg). Treatments were administered intraperitoneally and the gavage applied daily for 28 days. The sperm parameters, spermatogenesis index, total antioxidant capacity, testosterone level, and seminiferous tube diameter were assessed. Results: The values of all parameters reduced significantly in the DMN control group compared to the normal control group (p < 0.001). The PX and PX + DMN treatments at all entirely doses improved all parameters significantly compared to the DMN control group (p < 0.001). Conclusion: DMN caused detrimental effects on reproductive parameters. Also, no significant modifications were observed in PX treatments at all doses compared to the normal control group. PX compensated the toxic effect of DMN on reproductive parameters. Key words: Dimethyl nitrosamine, Reproductive, Pentoxifylline, Rat.

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