scholarly journals In vitro fibrolytic activity of the anaerobic fungus, Caecomyces sp., immobilized in alginate beads

2009 ◽  
Vol 18 (4) ◽  
pp. 758-768 ◽  
Author(s):  
R. Nagpal ◽  
A. Puniya ◽  
K. Singh
Keyword(s):  
Alginate Beads ◽  
Anaerobic Fungus

Characterization and Screening of a Novel Multiparticulate Pulsatile Delivery of Aceclofenac

2016 ◽  
Vol 9 (5) ◽  
pp. 3494-3501
Author(s):  
Bipul Nath ◽  
Santimoni Saikia
Keyword(s):  
Drug Release ◽  
Sodium Alginate ◽  
Alginate Beads ◽  
In Vitro Drug Release ◽  
Dsc Studies ◽  
Ft Ir ◽  
Lag Period ◽  
Pulsatile Delivery ◽  
Ca Alginate

In the present investigation, sodium alginate based multiparticulate system overcoated with time and pH dependent polymer was studied in the form of oral pulsatile system to achieve pulsatile with sustained release of aceclofenac for chronotherapy of rheumatoid arthritis seven batches of micro beads with varying concentration of sodium alginate (2-5 %) were prepared by ionotropic-gelation method using CaCl2 as cross-linking agent. The prepared Ca-alginate beads were coated with 5% Eudragit L100 and filled into pulsatile capsule with varying proportion of plugging materials. Drug loaded microbeads were investigated for physicochemical properties and drug release characteristics. The mean particle sizes of drug-loaded microbeads were found to be in the range 596±1.1 to 860 ± 1.2 micron and %DEE in the range of 65-85%. FT-IR and DSC studies revealed the absence of drug polymer interactions. The release of aceclofenac from formulations F1 to F7 in buffer media (pH 6.8) at the end of 5h was 65.6, 60.7, 55.7, 41.2, 39.2, 27 and 25% respectively. Pulsatile system filled with eudragit coated Ca-alginate microbeads (F2) showed better drug content, particle size, surface topography, in-vitro drug release in a controlled manner. Different plugging materials like Sterculia gum, HPMC K4M and Carbopol were used in the design of pulsatile capsule. The pulsatile system remained intact in buffer pH 1.2 for 2 hours due to enteric coat of the system with HPMCP. The enteric coat dissolved when the pH of medium was changed to 7.4. The pulsatile system developed with Sterculia gum as plugging material showed satisfactory lag period when compared to HPMC and Carbopol.

scholarly journals Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Stefano Focaroli ◽  
Gabriella Teti ◽  
Viviana Salvatore ◽  
Isabella Orienti ◽  
Mirella Falconi
Keyword(s):  
Tissue Engineering ◽  
Bone Morphogenetic Proteins ◽  
Transforming Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Biomechanical Properties ◽  
Alginate Beads ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Tissue Replacement

Articular cartilage is a highly organized tissue with complex biomechanical properties. However, injuries to the cartilage usually lead to numerous health concerns and often culminate in disabling symptoms, due to the poor intrinsic capacity of this tissue for self-healing. Although various approaches are proposed for the regeneration of cartilage, its repair still represents an enormous challenge for orthopedic surgeons. The field of tissue engineering currently offers some of the most promising strategies for cartilage restoration, in which assorted biomaterials and cell-based therapies are combined to develop new therapeutic regimens for tissue replacement. The current study describes thein vitrobehavior of human adipose-derived mesenchymal stem cells (hADSCs) encapsulated within calcium/cobalt (Ca/Co) alginate beads. These novel chondrogenesis-promoting scaffolds take advantage of the synergy between the alginate matrix and Co+2ions, without employing costly growth factors (e.g., transforming growth factor betas (TGF-βs) or bone morphogenetic proteins (BMPs)) to direct hADSC differentiation into cartilage-producing chondrocytes.

scholarly journals FABRICATION AND EVALUATION OF ORAL CONTROLLED RELEASE OF MUCOADHESIVE ALGINATE MICROBEADS CONTAINING FLUVASTATIN SODIUM

2019 ◽  
pp. 101-112
Author(s):  
VENKATA RAMANA REDDY K ◽  
NAGABHUSHANAM MV
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Transport Mechanism ◽  
Entrapment Efficiency ◽  
Alginate Beads ◽  
Polymer Mixture ◽  
Infrared Spectroscopic Study ◽  
Alginate Concentration ◽  
Curing Agents

Objective: The aim of this study is to prepare oral controlled release (CR) of mucoadhesive alginate microbeads encapsulating fluvastatin by gastroretention technology. Methods: The mucoadhesive microbeads containing fluvastatin were produced using emulsification internal gelation technique. The effect of different variables such as sodium alginate concentration and its combination with other hydrophilic polymers, and the effect of various curing agents on particle size, entrapment efficiency, and in vitro studies were evaluated. Results: There was no marked change in drug entrapment efficiency, and dissolution studies occur during the stability studies of fluvastatin. The in vitro results give data that improvement in the CR of the drug from microbeads compared with marketed tablet. Hence, in this regard, to minimize the frequency of drug administration to reduce side effects. The optimum condition for the preparation of stable alginate beads and produce CR manner was occurred at a higher concentration of combined polymer mixture in equal ratios, i.e., 3% w/v. Infrared spectroscopic study (Fourier transform infrared) confirmed the no incompatibility between drug and other excipients. X-ray diffraction study and differential scanning calorimetry were provided evidence that successful entrapment of drug into the alginates microbeads and drug converted into amorphous nature. The efficiency of mucoadhesion strength of microbeads was determined by wash-off study. Conclusion: The kinetic modeling of the release data indicates drug release from the microbeads follow anomalous transport mechanism and super Case-II transport mechanism. Drug release is a function of pH dependent and controlled drug release depends on type and concentration of polymer blend and curing agents. The release kinetics of drug from the alginate beads followed zero order.

scholarly journals Preparation and Characterization of Alginate and Psyllium Beads ContainingLactobacillus acidophilus

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Farzaneh Lotfipour ◽  
Shahla Mirzaeei ◽  
Maryam Maghsoodi
Keyword(s):  
Lactobacillus Acidophilus ◽  
Encapsulation Efficiency ◽  
Alginate Beads ◽  
Probiotic Bacteria ◽  
Partial Substitution ◽  
Narrow Size Distribution ◽  
Acidic Conditions ◽  
Ph Conditions

This paper describes preparation and characterization of beads of alginate and psyllium containing probiotic bacteria ofLactobacillus acidophilusDMSZ20079. Twelve different formulations containing alginate (ALG) and alginate-psyllium (ALG-PSL) were prepared using extrusion technique. The prepared beads were characterized in terms of size, morphology and surface properties, encapsulation efficiency, viabilities in acid (pH 1.8, 2 hours) and bile (0.5% w/v, 2 hours) conditions, and release in simulated colon pH conditions. The results showed that spherical beads with narrow size distribution ranging from1.59±0.04to1.67±0.09 mm for ALG and from1.61±0.06to1.80±0.07mm for ALG-PSL with encapsulation efficiency higher than 98% were achieved. Furthermore, addition of PSL into ALG enhanced the integrity of prepared beads in comparison with ALG formulations. The results indicated that incorporation of PSL into alginate beads improved viability of the bacteria in acidic conditions as well as bile conditions. Also, stimulating effect of PSL on the probiotic bacteria was observed through 20-hour incubation in simulated colonic pH solution. According to ourin vitrostudies, PSL can be a suitable polymer candidate for partial substitution with ALG for probiotic coating.

Tissue Engineered Human Septal Cartilage in a Murine Model

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P87-P88
Author(s):  
Angela Chang ◽  
Sage August ◽  
Barbara L Schumacher ◽  
Williams Gregory ◽  
Robert L Sah ◽  
...  
Keyword(s):  
Cell Viability ◽  
Murine Model ◽  
Three Dimensional ◽  
Alginate Beads ◽  
Septal Cartilage ◽  
Hematoxylin And Eosin ◽  
Matrix Production ◽  
Nasal Septal Cartilage

Problem Tissue engineering of human nasal septal cartilage represents an alternative technique for creating large quantities of autologous material for use in reconstructive surgery of the head and neck. Septal neocartilage constructs developed in vitro by the alginate method have demonstrated cartilaginous extracellular matrix production, but their biocompatibility and development in vivo remains largely unknown. Methods A murine model was used to examine the behavior of neocartilage constructs in vivo. Chondrocytes collected from donors undergoing septoplasty were expanded in monolayer and suspended in alginate beads for three-dimensional culture in media containing human serum and growth factors. After in vitro incubation for 5 weeks, the neocartilage constructs were implanted subcutaneously in the dorsum of athymic mice for 30 days (n=3). The mice were sacrificed and the constructs were explanted for assessment of cell viability, gross morphology, and histology. Results The mice survived and tolerated the implant well. Infection and extrusion were not observed. Neocartilage constructs maintained their general shape and size, and demonstrated cell viability after implantation. Explanted constructs were firm and opaque, sharing closer semblance to native septal tissue relative to the gelatinous, translucent pre-implant constructs. On hematoxylin and eosin staining, the explanted constructs exhibited distinct morphologies characteristic of native tissue, which were not observed in pre-implant constructs. Conclusion Neocartilage constructs are viable in an in vivo murine model. The morphologic and histologic features of explanted constructs more closely resemble native septal tissue when compared to pre-implant constructs. Significance Septal neocartilage constructs are biocompatible and demonstrate potential for in vivo maturation with eventual clinical application.

scholarly journals Development of coated beads for oral controlled delivery of cefaclor: In vitro evaluation

2013 ◽  
Vol 63 (1) ◽  
pp. 31-44 ◽  
Author(s):  
Bazigha K. Abdul ◽  
Sahar A. Fahmy
Keyword(s):  
Antimicrobial Activity ◽  
Controlled Release ◽  
Dosage Form ◽  
Size Range ◽  
Alginate Beads ◽  
Controlled Delivery ◽  
Peg 400 ◽  
Maximum Value ◽  
Plasma Profile

The aim of the present study was to develop and characterize coated chitosan-alginate beads containing cefaclor as a controlled release delivery system. Coated cefaclor beads were prepared by solvent evaporation techniques. Beads were found to be intact and spherical in shape. Their size range was 1.05 to 2.06. The loading efficiency showed maximum value when the concentration of cefaclor, chitosan and PEG 400 was 10 % (m/V), 0.5 % (m/V) and 2 % (V/V), respectively. Best retardation of cefaclor release from chitosan-alginate beads was achieved by coating with 15 % of shellac in formula F19. A significant antimicrobial activity (p < 0.05) against Staphylococcus aureus and Klebsiella pneumoniae was observed for formula F19 compared to the standard antibiotic disc. Furthermore, the simulated plasma profile showed the superiority of F19 in sustaining drug release for more than 12 h. Therefore, shellac coated chitosan-alginate beads could be considered a successful controlled release oral cefaclor dosage form.

Calcium alginate beads as a slow-release system for delivering angiogenic molecules In Vivo and In Vitro

1992 ◽  
Vol 152 (2) ◽  
pp. 422-429 ◽  
Author(s):  
Elizabeth C. Downs ◽  
Nancy E. Robertson ◽  
Terry L. Riss ◽  
Marian L. Plunkett
Keyword(s):  
Calcium Alginate ◽  
Slow Release ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
Release System ◽  
Slow Release System

Therapeutic Effects of Paclitaxel-Loaded Hydroxyapatite-Alginate Beads: In Vitro and In Vivo Studies

2008 ◽  
Vol 396-398 ◽  
pp. 551-554 ◽  
Author(s):  
Tetsuya Abe ◽  
Masataka Sakane ◽  
Toshiyuki Ikoma ◽  
Mihoko Kobayashi ◽  
Naoyuki Ochiai
Keyword(s):  
Cytotoxic Activity ◽  
Survival Time ◽  
Alginate Beads ◽  
Free Time ◽  
Mammary Adenocarcinoma ◽  
Therapeutic Effects ◽  
Composite Beads ◽  
Disease Free

Several drug delivery carriers have reported on local delivery of paclitaxel (PTX), but their effects on intraosseous cancer model are not well known. This study was conducted to clarify the therapeutic effects of our newly developed PTX-loaded HAp-alginate composite beads. Cytotoxic activity was assessed on rat’s mammary adenocarcinoma by cell proliferation assay using WST-1 reagent. Antitumor activity was assessed by 8-week-old rat female Fischer 344 rats of metastatic spine cancer. Twenty-three rats were divided into 3 groups: Group 1 (n = 7) and Group 2 (n = 8) was treated with the PTX-loaded HAp-alginate beads using strontium ions and barium ions, respectively. Group 3 (n = 8) was administered with drug-free HAp-alginate beads. We checked disease-free time and survival time among 3 groups. The HAp-alginate beads containing 2.4wt% of PTX showed significant cytotoxic activity on CRL-1666 cells. The effects were decreased with time during 72 h. The animals treated with 2.4wt% of PTX-loaded HAp-alginate beads showed 40% increase in the disease-free time and 25% increase in survival time. Our studies suggest that newly developed HAp-alginate beads can be a candidate carrier of PTX to bone.

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