scholarly journals Formulation and Evaluation of Mangosteen (Garcinia mangostana L.) Fruit Pericarp Extract Gel

2021 ◽  
Vol 11 (2) ◽  
pp. 90-97
Author(s):  
Rina Kuswahyuning ◽  
Indra Lesmana
Keyword(s):  
Skin Disorder ◽  
In Vitro Study ◽  
Scavenging Activity ◽  
Garcinia Mangostana ◽  
Topical Gel ◽  
Skin Delivery ◽  
Dpph Scavenging Activity ◽  
Fruit Pericarp ◽  
Dpph Scavenging

The topical antioxidant product may be useful for the treatment of oxidative stress-related skin disorder. This research aimed to evaluate a topical gel formulation of Garcinia mangostana L. fruit pericarp (GMP) extract. GMP extract was formulated into a gel and characterized for its physical properties. The antioxidant activity was evaluated based on the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The ability of gel formulation to release GMP extract and promote skin delivery was estimated based on DPPH scavenging method and also compared to that of suspension form. The results showed that the GMP extract gel showed characteristics for topical application. The radical DPPH scavenging activity was confirmed both in GMP extract and GMP extract gel. In vitro study release showed that the GMP extract was released from gel. Some degree of GMP extract was also delivered into the shed snakeskin in vitro. When compared with the suspension form, GMP extract gel provided a more profoundly lower release.

scholarly journals Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Enqin Xia ◽  
Lu Zhai ◽  
Zhigang Huang ◽  
Hairong Liang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Protein Extraction ◽  
Amino Acid Sequences ◽  
Ultrasound Wave ◽  
Scavenging Activity ◽  
Gastrointestinal Digestion ◽  
Ultrasound Assisted Extraction ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L−1 NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated in vitro digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of Dunaliella salina could be a potential source of antioxidative peptides through simulated in vitro gastrointestinal digestion.

scholarly journals In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 258-262
Author(s):  
M. Narmatha ◽  
S. Maneemegalai
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Syzygium Cumini ◽  
Total Alkaloids ◽  
Dpph Scavenging Activity ◽  
Inhibitory Activities ◽  
Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.  

scholarly journals Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

2021 ◽  
Vol 15 (6) ◽  
pp. 513-528
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingöl ◽  
Ahmet C. Gören ◽  
Saleh H. Alwasel ◽  
İlhami Gülçin
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

scholarly journals Evaluation of in-Vitro antioxidant and antidiarrheal activities of Peperomia Pellucida methanol extracts on Albino mice

2021 ◽  
Vol 25 (9) ◽  
pp. 1681-1688
Author(s):  
O.D. Uwaya ◽  
P.O. Omozuwa ◽  
R.E. Inegbedion
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Tropical Regions ◽  
Cholesterol Levels ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Common Plant

Peperomia pellucida also known as shiny bush is a common plant wildly distributed in the tropical regions of the world including Nigeria. In ethnomedicine, it is used to treat hemorrhages, fevers, lower cholesterol levels and serves as a cough suppressant, emollient, and diuretic. This study evaluated the in vitro and antidiarrhea activities of Peperomia pellucida. For the in vitro antioxidant assay the following methods were used: 2-diphenyl-2-pierylhydrazylhydrate (DPPH) scavenging activity, Superoxide scavenging activity, Hydrogen peroxide scavenging activity, Lipid Peroxidation Assay, Total Antioxidant Capacity, 2,2’-Azinobis (3-Ethylbenzothiazoline-6-sulphonic Acid) (ABTS) radical scavenging activity and Hydroxyl radical scavenging activity. Antidiarrheal activities were studied using Castor Oil-Induced and Intestinal Transit in Mice models. Methanol and aqueous plant extract of Peperomia pellucida at various concentration demonstrated impressive in vitro antioxidant scavenging activities. The onset of diarrheal (**P<0.01) and the % of charcoal travel (*P<0.05) in the mice was reduced at 200 mg/kg compared to the control. Conclusively, this study showed that the aqueous and methanol extract of Peperomia pellucida has in vitro free radical scavenging activities. The plant demonstrated antidiarrheal activities.

scholarly journals Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 420
Author(s):  
Tijana Kolarević ◽  
Danijel D. Milinčić ◽  
Tatjana Vujović ◽  
Uroš M. Gašić ◽  
Ljiljana Prokić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Blueberry Leaves

The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/l IBA and 0.1 mg/l GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.

scholarly journals Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1825 ◽  
Author(s):  
Yuanyang Dong ◽  
Qihang Hou ◽  
Meng Sun ◽  
Jingjing Sun ◽  
Bingkun Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Plantago Asiatica ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function of Plantago asiatica L. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in cells. Hydrogen-peroxide-induced oxidative stress led to decreased cell viability, impaired intercellular junction, and damage to the cell membrane and DNA. Furthermore, aesculetin ameliorated decreased cell viability induced by hydrogen peroxide via upregulation of antioxidant related genes, and apigenin also protected against H2O2 mainly by improving the glutathione (GSH) antioxidant system, such as increasing the activity of glutathione peroxidase (GPX), glutathione reductase (GR), and the ration of GSH/glutathione disulfide (GSSG). Above all, these findings suggest that aesculetin and apigenin may be bioactive compounds for antioxidant function in Plantago asiatica L.

Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Estelle N.H. Youl ◽  
Cyrille A.P. Ouédraogo ◽  
Moustapha Gambo ◽  
Moussa Ouédraogo ◽  
Martin Kiendrebéogo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Burkina Faso ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ziziphus Mauritiana ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract Background Ziziphus mauritiana Lam. is a plant used in traditional medicine in Burkina Faso in the treatment of several diseases, of which diabetes is characterized by oxidative stress. The aim of this study was to evaluate the in vitro antioxidant potential of the extracts of leaves of this plant. Methods The crude hydroethanolic extract (HEE) of the leaves of Z. mauritiana and their partitionates in n-hexane, dichloromethane, and ethyl acetate, and in the residual aqueous solution (the F1, F2, F3, and F4 fractions, respectively) were first prepared. The content of polyphenols was determined and the antioxidant effects of the extracts were evaluated by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, inhibition of lipid peroxidation (TBARS), and the ferric reducing antioxidant power (FRAP). Results The HEE as well as the F3 and F4 fractions were rich in polyphenols with contents between 58 and 84 mg equivalent gallic acid per 100 mg. The flavonoid content was 4 mg quercetin equivalents in the HEE and the F4 fraction. Except for the F1 fraction, the HEE and the other fractions showed significant DPPH scavenging activity (IC50 between 8 and 12 μg/mL). The IC50 of TBARS by different extracts was in the range 1–5 μg/mL, and the FRAP activity was 7–85 mg ascorbic acid equivalent per 100 mg. Total polyphenol content was highly correlated with the antioxidant activities. Conclusions The HEE, F3, and F4 fractions were found to be the richest in polyphenols and had the best antioxidant activity. The antioxidant activity of the extracts of the leaves of Z. mauritiana is due to these polyphenolic compounds.

scholarly journals Characterization of Antioxidant Peptides from Thai Traditional Semi-Dried Fermented Catfish

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 262
Author(s):  
Manat Chaijan ◽  
Tanutchaporn Rodsamai ◽  
Sawanya Charoenlappanit ◽  
Sittiruk Roytrakul ◽  
Atikorn Panya ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heating Temperature ◽  
Clarias Gariepinus ◽  
Antioxidant Properties ◽  
In Vitro Digestion ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Herein, the antioxidant peptides from a Thai traditional semi-dried fermented farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) catfish, Pla Duk Ra, were characterized. After extraction and deproteinization, Pla Duk Ra crude peptide extract (CPE) was fractioned using 2 connected Hitrap Sephadex-G25 columns, yielding two significant fractions, F1 with higher browning intensity (A420) and F2. CPE, F1, and F2 had different amino acid profiles, contents, and sequences evaluated by LC-MS/MS, which could be responsible for their antioxidant properties. F2 contained the highest numbers of hydrophobic amino acid (HBA) (47.45%) and aromatic amino acid (27.31%), followed by F1, and CPE. The peptides with 8–24 amino acid residues were detected in CPE and its fractions. In CPE, F1, and F2, there were 69, 68, and 85 peptides with varied HBA content, respectively. ARHSYGMLYCSCPPND (50% HBA), ALRKMGRK (37.5% HBA), and ANWMIPLM (87.5% HBA) were the most prevalent peptides found in CPE, F1, and F2. Overall, F2 was the most effective at inhibiting free radicals (DPPH● and ABTS●+) and reactive oxygen species (hydroxyl radical, singlet oxygen, and hydrogen peroxide), followed by F1 and CPE. The metal chelation of F1 was, however, superior to that of F2 and CPE. For the stability test, the effects of pH, heating temperature, and in vitro digestion on the DPPH● scavenging activity of F2 were investigated. The activity was boosted by lowering the pH and raising the heating temperature. In the gastrointestinal tract model system, however, roughly 50% of DPPH● scavenging activity reduced after digesting.

scholarly journals Effective Antioxidant Activities of Anthocyanins as Affected by pH of Antioxidant Assays

2018 ◽  
Vol 16 (11) ◽  
pp. 875-885
Author(s):  
Paradorn NGAMDEE ◽  
Sudarat JIAMYANGYUEN
Keyword(s):  
Antioxidant Activities ◽  
Anthocyanin Content ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Total Anthocyanin ◽  
Antioxidant Assays ◽  
Buffer Solutions ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

This study analyzed the effect of pH on antioxidant activities of anthocyanins. Five black glutinous rice bran crude extracts (BBCs) with different anthocyanin content were compared. They were referred to as Moo Ser (MS), Saked (SK), Phayao (PY), Phichit (PC) and Kao Kor (KK). The antioxidant activities were evaluated using 2 in vitro antioxidant assays conducted at different pH, DPPH (pH 5.5) and FRAP (pH 3.6). Total anthocyanin content (TAC) of KK (23 g/kg) was the highest, followed by PC (13 g/kg), PY and SK (both 11 g/kg), and MS (10 g/kg). The pH of the assays affected the activity of the anthocyanins as the KK showed DPPH scavenging activity comparable to other BBCs but exhibited significantly greater FRAP antioxidant activity. To eliminate possible interference by other active components in the BBC, anthocyanin-rich isolate (ARI) was then isolated from KK. The ARI contained 90 % cyanidin-3-glucoside as analyzed by HPLC. The ARI was dissolved in 5 different buffer solutions (pH 1, 3, 5, 7, and 9) and incubated for 1 h, 3 days, and 1 week before the evaluation of antioxidant activities. All ARI-containing buffer solutions exhibited comparable DPPH scavenging activity. However, the ones at pH 5 and 7 solutions were less reactive than others when tested with the FRAP assay. These results indicated that anthocyanin is a good antioxidant not only in an acidic environment but also in higher pH ranges. This suggests that the utilization of anthocyanins as natural antioxidants in products such as food and cosmetics is not limited by the pH of the products.

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