scholarly journals Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1825 ◽  
Author(s):  
Yuanyang Dong ◽  
Qihang Hou ◽  
Meng Sun ◽  
Jingjing Sun ◽  
Bingkun Zhang
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Plantago Asiatica ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Plantago asiatica L. is widely distributed in Eastern Asia and a commonly used drug in China, Korea, and Japan for diuretic and antiphlogistic purposes. In this experiment, the present study was performed to isolate antioxidant molecules based on the DPPH scavenging activity assay and discover the bioactive compounds which contributed to performing the function of Plantago asiatica L. Each faction was chosen for further isolation guided by DPPH scavenging activity assay. Afterwards, two potential bioactive molecules, aesculetin and apigenin, were isolated for in vitro antioxidant activity in cells. Hydrogen-peroxide-induced oxidative stress led to decreased cell viability, impaired intercellular junction, and damage to the cell membrane and DNA. Furthermore, aesculetin ameliorated decreased cell viability induced by hydrogen peroxide via upregulation of antioxidant related genes, and apigenin also protected against H2O2 mainly by improving the glutathione (GSH) antioxidant system, such as increasing the activity of glutathione peroxidase (GPX), glutathione reductase (GR), and the ration of GSH/glutathione disulfide (GSSG). Above all, these findings suggest that aesculetin and apigenin may be bioactive compounds for antioxidant function in Plantago asiatica L.

scholarly journals Optimization and Identification of Antioxidant Peptide from Underutilized Dunaliella salina Protein: Extraction, In Vitro Gastrointestinal Digestion, and Fractionation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Enqin Xia ◽  
Lu Zhai ◽  
Zhigang Huang ◽  
Hairong Liang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Dunaliella Salina ◽  
Protein Extraction ◽  
Amino Acid Sequences ◽  
Ultrasound Wave ◽  
Scavenging Activity ◽  
Gastrointestinal Digestion ◽  
Ultrasound Assisted Extraction ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

DPPH• scavenging peptides (<3kDa) from underutilized Dunaliella salina protein were obtained by the following successive treatment, i.e., ultrasound extraction, simulated in vitro gastrointestinal digestion hydrolyzation, and membrane ultrafiltration classification. The optimal condition for ultrasound-assisted extraction was an ultrasound wave with 800 W of power treating a mixture of 60 mL of 1.0 mol L−1 NaOH and 2 g algae powder for 15 min. A high correlation (r=0.8146) between DPPH• scavenging activity and yield of the intact peptides showed their antioxidant capacity. Simulated in vitro digestion assay resulted in excellent DPPH• scavenging activity of the total peptide, amounting to (86.5 ± 10.1)%, comparing with the nondigestion samples at (46.8 ± 6.5)%. After fractionation, the 500-1000 Da fraction exhibited the highest DPPH• scavenging activity (81.2 ± 4.0)%, increasing 1.5 times due to digestion. Then, the 500-1000 Da fraction was analyzed by RPLC-Q Exactive HF mass spectrometer, and 4 novel peptides, i.e., Ile-Leu-Thr-Lys-Ala-Ala-Ile-Glu-Gly-Lys, Ile-Ile-Tyr-Phe-Gln-Gly-Lys, Asn-Asp-Pro-Ser-Thr-Val-Lys, and Thr-Val-Arg-Pro-Pro-Gln-Arg, were identified. From these amino acid sequences, hydrophobic residues accounted for 56%, which indicated their high antioxidant property. The results indicated that underutilized protein of Dunaliella salina could be a potential source of antioxidative peptides through simulated in vitro gastrointestinal digestion.

scholarly journals Formulation and Evaluation of Mangosteen (Garcinia mangostana L.) Fruit Pericarp Extract Gel

2021 ◽  
Vol 11 (2) ◽  
pp. 90-97
Author(s):  
Rina Kuswahyuning ◽  
Indra Lesmana
Keyword(s):  
Skin Disorder ◽  
In Vitro Study ◽  
Scavenging Activity ◽  
Garcinia Mangostana ◽  
Topical Gel ◽  
Skin Delivery ◽  
Dpph Scavenging Activity ◽  
Fruit Pericarp ◽  
Dpph Scavenging

The topical antioxidant product may be useful for the treatment of oxidative stress-related skin disorder. This research aimed to evaluate a topical gel formulation of Garcinia mangostana L. fruit pericarp (GMP) extract. GMP extract was formulated into a gel and characterized for its physical properties. The antioxidant activity was evaluated based on the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The ability of gel formulation to release GMP extract and promote skin delivery was estimated based on DPPH scavenging method and also compared to that of suspension form. The results showed that the GMP extract gel showed characteristics for topical application. The radical DPPH scavenging activity was confirmed both in GMP extract and GMP extract gel. In vitro study release showed that the GMP extract was released from gel. Some degree of GMP extract was also delivered into the shed snakeskin in vitro. When compared with the suspension form, GMP extract gel provided a more profoundly lower release.

scholarly journals In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 258-262
Author(s):  
M. Narmatha ◽  
S. Maneemegalai
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Syzygium Cumini ◽  
Total Alkaloids ◽  
Dpph Scavenging Activity ◽  
Inhibitory Activities ◽  
Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.  

scholarly journals Lactic Acid Fermented Green Tea with Levilactobacillus brevis Capable of Producing γ-Aminobutyric Acid

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 110
Author(s):  
Young Hun Jin ◽  
Jong Hyoung Hong ◽  
Jun-Hee Lee ◽  
Hyeock Yoon ◽  
Alixander Mattay Pawluk ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Green Tea ◽  
Bioactive Compounds ◽  
Growth Potential ◽  
Rrna Gene ◽  
Scavenging Activity ◽  
Gaba Production ◽  
Green Tea Leaves ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The antioxidative activity and bioactive compounds content of lactic acid fermented green tea (LFG) fermented with an outstanding GABA-producing strain under optimised fermentation conditions were evaluated. Levilactobacillus strain GTL 79 was isolated from green tea leaves and selected based on acid production, growth potential, catechin resistance, and GABA production to be applied to LFG. Through 16S rRNA gene sequence analysis, the strain was identified as Levilactobacillus brevis. The optimised conditions were defined as fermentation at 37 °C with supplementation of 1% fermentation alcohol, 6% glucose, and 1% MSG and was determined to be most effective in increasing the lactic acid, acetic acid, and GABA content in LFG by 522.20%, 238.72% and 232.52% (or 247.58%), respectively. Initial DPPH scavenging activity of LFG fermented under the optimised conditions was 88.96% and rose to 94.38% by day 5. Polyphenols may contribute to the initial DPPH scavenging activity, while GABA and other bioactive compounds may contribute to the activity thereafter. Consequently, as GABA and other bioactive compounds found in green tea have been reported to have health benefits, future studies may prove that optimally fermented LFG by L. brevis GTL 79 could be useful in the food and health industries.

scholarly journals Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

2021 ◽  
Vol 15 (6) ◽  
pp. 513-528
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingöl ◽  
Ahmet C. Gören ◽  
Saleh H. Alwasel ◽  
İlhami Gülçin
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

scholarly journals Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay

2019 ◽  
pp. 1-6
Author(s):  
Alhassan M. Garba ◽  
Habiba R. Isa ◽  
Sadiq Abubakar ◽  
Saudat Ja’afar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Dried and powdered leaves of Albizia chevalieri were extracted using ethanol. The extract was fractionated to give methanol, chloroform and pet-ether. The four extracts obtained; ethanol, chloroform, methanol and pet-ether were evaluated for antioxidant activity using 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) free radical scavenging activity assay. The results of the DPPH scavenging activity indicated a concentration-dependent antioxidant activity. The DPPH scavenging activity of the ethanol, chloroform and methanol extracts were found to be promising. There is no significant difference in the antioxidant activity between the ethanol, chloroform and methanol extracts with that of standard Ascorbic acid at 10, 25, 250 and 500 μg/ml concentrations. This showed that the ethanol, chloroform and methanol leave extracts of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.

scholarly journals Evaluation of in-Vitro antioxidant and antidiarrheal activities of Peperomia Pellucida methanol extracts on Albino mice

2021 ◽  
Vol 25 (9) ◽  
pp. 1681-1688
Author(s):  
O.D. Uwaya ◽  
P.O. Omozuwa ◽  
R.E. Inegbedion
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Tropical Regions ◽  
Cholesterol Levels ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Common Plant

Peperomia pellucida also known as shiny bush is a common plant wildly distributed in the tropical regions of the world including Nigeria. In ethnomedicine, it is used to treat hemorrhages, fevers, lower cholesterol levels and serves as a cough suppressant, emollient, and diuretic. This study evaluated the in vitro and antidiarrhea activities of Peperomia pellucida. For the in vitro antioxidant assay the following methods were used: 2-diphenyl-2-pierylhydrazylhydrate (DPPH) scavenging activity, Superoxide scavenging activity, Hydrogen peroxide scavenging activity, Lipid Peroxidation Assay, Total Antioxidant Capacity, 2,2’-Azinobis (3-Ethylbenzothiazoline-6-sulphonic Acid) (ABTS) radical scavenging activity and Hydroxyl radical scavenging activity. Antidiarrheal activities were studied using Castor Oil-Induced and Intestinal Transit in Mice models. Methanol and aqueous plant extract of Peperomia pellucida at various concentration demonstrated impressive in vitro antioxidant scavenging activities. The onset of diarrheal (**P<0.01) and the % of charcoal travel (*P<0.05) in the mice was reduced at 200 mg/kg compared to the control. Conclusively, this study showed that the aqueous and methanol extract of Peperomia pellucida has in vitro free radical scavenging activities. The plant demonstrated antidiarrheal activities.

scholarly journals Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 420
Author(s):  
Tijana Kolarević ◽  
Danijel D. Milinčić ◽  
Tatjana Vujović ◽  
Uroš M. Gašić ◽  
Ljiljana Prokić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Blueberry Leaves

The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/l IBA and 0.1 mg/l GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.

Bioactive Compounds and Antioxidant Capacity of Exotic Fruits and Commercial Frozen Pulps from Brazil

2008 ◽  
Vol 14 (3) ◽  
pp. 207-214 ◽  
Author(s):  
M.I. Genovese ◽  
M. Da Silva Pinto ◽  
A.E. De Souza Schmidt Gonçalves ◽  
F.M. Lajolo
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Scavenging Capacity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Exotic Fruits ◽  
Β Carotene

The objective of this work was to characterize exotic fruits (cambuci, araça-boi, camu-camu, jaracatia, araça) and commercial frozen pulps (araça, cambuci, umbu, coquinho, pana, native passion fruit, cagaita) from Brazil in relation to their bioactive compounds contents and antioxidant capacity. Camu-camu (Myrciaria dubia) presented the highest vitamin C and total phenolics contents (397 and 1797 mg/100 gf.w., respectively) and the highest DPPH• scavenging capacity. Coquinho (Butia capitata) also showed a significant vitamin C content (43 mg/100 gf.w.). Among the commercial frozen pulps, cagaita presented the higher DPPH scavenging activity and inhibition of β-carotene bleaching. A good correlation between total phenols and DPPH scavenging activity was found for fruits (r = 0.997) and commercial frozen pulps (r = 0.738). However, no correlation was found for total phenols and inhibition of β-carotene bleaching. Quercetin and kaempferol derivatives were the main flavonoids present in all samples and cyanidin derivatives were detected only in camu-camu. Camu-camu and araça (Psidium guineensis) showed the highest total ellagic acid contents (48 and 63.5 mg/100 gf.w.). All commercial frozen pulps presented lower contents of bioactive compounds and antioxidant capacity than their respective fruits. According to our results, camu-camu and araça might be sources of bioactive compounds.

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