Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Estelle N.H. Youl ◽  
Cyrille A.P. Ouédraogo ◽  
Moustapha Gambo ◽  
Moussa Ouédraogo ◽  
Martin Kiendrebéogo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Burkina Faso ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Ziziphus Mauritiana ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract Background Ziziphus mauritiana Lam. is a plant used in traditional medicine in Burkina Faso in the treatment of several diseases, of which diabetes is characterized by oxidative stress. The aim of this study was to evaluate the in vitro antioxidant potential of the extracts of leaves of this plant. Methods The crude hydroethanolic extract (HEE) of the leaves of Z. mauritiana and their partitionates in n-hexane, dichloromethane, and ethyl acetate, and in the residual aqueous solution (the F1, F2, F3, and F4 fractions, respectively) were first prepared. The content of polyphenols was determined and the antioxidant effects of the extracts were evaluated by their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, inhibition of lipid peroxidation (TBARS), and the ferric reducing antioxidant power (FRAP). Results The HEE as well as the F3 and F4 fractions were rich in polyphenols with contents between 58 and 84 mg equivalent gallic acid per 100 mg. The flavonoid content was 4 mg quercetin equivalents in the HEE and the F4 fraction. Except for the F1 fraction, the HEE and the other fractions showed significant DPPH scavenging activity (IC50 between 8 and 12 μg/mL). The IC50 of TBARS by different extracts was in the range 1–5 μg/mL, and the FRAP activity was 7–85 mg ascorbic acid equivalent per 100 mg. Total polyphenol content was highly correlated with the antioxidant activities. Conclusions The HEE, F3, and F4 fractions were found to be the richest in polyphenols and had the best antioxidant activity. The antioxidant activity of the extracts of the leaves of Z. mauritiana is due to these polyphenolic compounds.

scholarly journals Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula orientalis L. Determination of Its Polyphenol Contents by LC-HRMS

2021 ◽  
Vol 15 (6) ◽  
pp. 513-528
Author(s):  
Hatice Kızıltaş ◽  
Zeynebe Bingöl ◽  
Ahmet C. Gören ◽  
Saleh H. Alwasel ◽  
İlhami Gülçin
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS•+ scavenging activity, DPPH· scavenging activity, Fe3+reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, α-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 µg/mL against acetylcholinesterase (AChE), 0.815 µg/mL against α-glycosidase, and 0.675 µg/mL against α-amylase.

scholarly journals Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Carla Nicola ◽  
Mirian Salvador ◽  
Adriana Escalona Gower ◽  
Sidnei Moura ◽  
Sergio Echeverrigaray
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Neurodegenerative Diseases ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Alkaloid Content ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloidm/z385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50= 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50= 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50= 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

scholarly journals In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 258-262
Author(s):  
M. Narmatha ◽  
S. Maneemegalai
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Syzygium Cumini ◽  
Total Alkaloids ◽  
Dpph Scavenging Activity ◽  
Inhibitory Activities ◽  
Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.  

scholarly journals Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
THEERAPHAN CHUMROENPHAT ◽  
Surapon Saensouk ◽  
PIYAPORN SAENSOUK
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii,  TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

scholarly journals In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

2012 ◽  
Vol 62 (3) ◽  
pp. 371-382 ◽  
Author(s):  
Jean Paul Kamdem ◽  
Sílvio Terra Stefanello ◽  
Aline Augusti Boligon ◽  
Caroline Wagner ◽  
Ige Joseph Kade ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
High Concentrations ◽  
Dpph Scavenging

Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL-1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g-1 of dry extract. Fe2+-induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca2+-induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in a concentration-dependent manner. Ethanolic extract reduced mitochondrial Dym only at high concentrations (40-100 mg mL-1), which indicates that its toxicity does not overlap with its antioxidant effects. Results suggest involvement of antioxidant activities of T. catigua in its pharmacological properties.

scholarly journals Presumptive Relationship between Oxidoreduction Potential and Both Antibacterial and Antioxidant Activities of Herbs and Spices: Oxidoreduction Potential as a Companion Tool for Measuring the Antioxidant Activity

2018 ◽  
Vol 47 (2) ◽  
pp. 506-514 ◽  
Author(s):  
Duried ALWAZEER ◽  
Sally DHAM
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Oxidoreduction Potential ◽  
Herbs And Spices

Antibacterial and antioxidant activities form an essential component of the bioactive properties of plant products. The antibacterial and the antioxidant properties of three types of aqueous extracts of four herbs and spices were evaluated. While the total phenolic content, DPPH radical scavenging activity and oxidoreduction potential methods were used for the antioxidant properties determination, the inhibition diameters of extracts toward the growth of Staphylococcus warneri, Bacillus cereus, Enterobacter cloacae and Proteus vulgaris were evaluated. Although the phenolic content did not correlate with the antibacterial activity of plant extracts, it linearly correlated (R2 = 0.827) with DPPH scavenging activity of the studied plants following the order: sumac > ginger > rosemary > cinnamon. The oxidoreduction potential values linearly correlated with both the phenolic content (R2 ≈ 0.88) and DPPH scavenging activity values (R2 ≈ 0.96). The oxidoreduction potential could be proposed as a useful companion tool combined with other techniques when determining the antioxidant activity of plant extracts and food products is considered.

Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae

2021 ◽  
pp. 6447-6454
Author(s):  
Hana R. Bajes ◽  
Sawsan A. Oran ◽  
Yasser K. Bustanji
Keyword(s):  
Antioxidant Activity ◽  
Cancer Cells ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Human Fibroblasts ◽  
Wild Plant ◽  
Scavenging Activity ◽  
Medicinal Uses ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.

scholarly journals The Antioxidant and Cytotoxic Effects of Cosmos caudatus Ethanolic Extract on Cervical Cancer

2018 ◽  
Vol 10 (3) ◽  
pp. 243-9 ◽  
Author(s):  
Betty Nurhayati ◽  
Ira Gustira Rahayu ◽  
Sonny Feisal Rinaldi ◽  
Wawan Sofwan Zaini ◽  
Ervi Afifah ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Anticancer Activity ◽  
Ethanolic Extract ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Cosmos Caudatus ◽  
Activity Test ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

BACKGROUND: Oxidative stress is closely related to all aspects of cancer. Cosmos caudatus ethanolic extract (CCEE) has been proved to have antioxidant effect that inhibited cancer cell growth due to its bioactive compounds such as catechin, quercetin and chlorogenic acid. This study aimed to evaluate antioxidant and anticancer activity of CCEE and its compounds.METHODS: Total phenol was measured according to the Folin-Ciocalteu method. Catechin, quercetin and chlorogenic acid contained in CCEE were identified by high-performance liquid chromatography (HPLC). Antioxidant activity was evaluated by 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)-reducing activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and ferric reducing antioxidant power (FRAP) activity test. The cytotoxic activity of CCEE was determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on HeLa cells.RESULTS: The result showed that total phenol of CCEE was 181.64±0.93 µg Cathecin/mg extract. ABTSreducing activity test showed that catechin had the highest activity (2.90±0.04 µg/mL), while CCEE had moderate activity compared to other compounds. FRAP activity test demonstrated that catechin had the highest activity (315.83 µM Fe(II)/µg) compared to other compounds. DPPH scavenging activity of CCEE was 22.82±0.05 µg/mL. Cytotoxicity test on HeLa cell showed that CCEE had lower activity (inhibitory concentration (IC)50= 89.90±1.30 µg/mL) compared to quercetin (IC50 = 13.30±0.64 µg/ mL).CONCLUSION: CCEE has the lowest antioxidant activity compared to quercetin, catechin, and chlorogenic acid and has the lowest anticancer activity compared to quercetin. However, CCEE and its compounds has potential as antioxidant and anticancer properties.KEYWORDS: antioxidant, anticancer, catechin, Cosmos caudatus, quercetin

scholarly journals Effective Antioxidant Activities of Anthocyanins as Affected by pH of Antioxidant Assays

2018 ◽  
Vol 16 (11) ◽  
pp. 875-885
Author(s):  
Paradorn NGAMDEE ◽  
Sudarat JIAMYANGYUEN
Keyword(s):  
Antioxidant Activities ◽  
Anthocyanin Content ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Total Anthocyanin ◽  
Antioxidant Assays ◽  
Buffer Solutions ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

This study analyzed the effect of pH on antioxidant activities of anthocyanins. Five black glutinous rice bran crude extracts (BBCs) with different anthocyanin content were compared. They were referred to as Moo Ser (MS), Saked (SK), Phayao (PY), Phichit (PC) and Kao Kor (KK). The antioxidant activities were evaluated using 2 in vitro antioxidant assays conducted at different pH, DPPH (pH 5.5) and FRAP (pH 3.6). Total anthocyanin content (TAC) of KK (23 g/kg) was the highest, followed by PC (13 g/kg), PY and SK (both 11 g/kg), and MS (10 g/kg). The pH of the assays affected the activity of the anthocyanins as the KK showed DPPH scavenging activity comparable to other BBCs but exhibited significantly greater FRAP antioxidant activity. To eliminate possible interference by other active components in the BBC, anthocyanin-rich isolate (ARI) was then isolated from KK. The ARI contained 90 % cyanidin-3-glucoside as analyzed by HPLC. The ARI was dissolved in 5 different buffer solutions (pH 1, 3, 5, 7, and 9) and incubated for 1 h, 3 days, and 1 week before the evaluation of antioxidant activities. All ARI-containing buffer solutions exhibited comparable DPPH scavenging activity. However, the ones at pH 5 and 7 solutions were less reactive than others when tested with the FRAP assay. These results indicated that anthocyanin is a good antioxidant not only in an acidic environment but also in higher pH ranges. This suggests that the utilization of anthocyanins as natural antioxidants in products such as food and cosmetics is not limited by the pH of the products.

scholarly journals An Assessment of Total Polyphenolic Content and Antioxidant Potential of Mauby Bark Extracts (Colubrina arborescens) Brewed for Different Lengths of Time

2019 ◽  
Vol 8 (3) ◽  
pp. 26
Author(s):  
Jeremy Embola ◽  
Cheryl Rock ◽  
Long Wang ◽  
Wendy Reiboldt ◽  
Saba Ahmed ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Polyphenolic Content ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Total Polyphenolic Content ◽  
The Caribbean ◽  
Dpph Scavenging

Mauby bark (Colubrina arborescens) is commonly used to make a beverage,“Mauby”, in the Caribbean and is believed to possess antiglycemic, antilipidemic, and anticarcinogenic properties. However, limited studies have been conducted to substantiate the compounds present that may confer these benefits. Therefore, the objectives of this research were to quantify the total polyphenolic content and evaluate the antioxidant capacity of Mauby bark extracts brewed in water at 30, 45, and 60 minutes. In the extracts, the Total Flavonoid Content (TFC) ranged from 1.93 - 3.17 mg CE/mL and the Total Phenolic Content (TPC) ranged from 2.10 mg ± 0.11 GAE/mL (45 minutes) - 2.36 mg ± 0.067 GAE/mL (30 minutes). Moreover, their antioxidant activity was assessed using the 2,2 Diphenyl 1-Picrylhydrazyl (DPPH) and  Ferric Reducing Antioxidant Power (FRAP) assays. The DPPH scavenging activity observed from Mauby extracts ranged from 75% ± 4.02 (30 minutes) to 83% ± 0.66 (60 minutes) and the FRAP values ranged from 6.29± 0.84 (30 minutes) to 6.90 ± 1.54 mM FeSO4 equivalents/ 0.2 mL Mauby extract (45 minutes). Although, polyphenolic content at 30 minutes was greater than 60 minutes of brewing for TFC (p < 0.001) and TPC (p = 0.002), the scavenging activity was greater at 60 minutes than 30 minutes (p = 0.014) while antioxidant power was not affected by brewing time (p = 0.736). In summary, brewing the bark at 60 minutes was observed to provide the highest antioxidant activity.

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