scholarly journals Aktivitas Antioksidan serta Penghambatan HMG CoA dan Lipase dari Kombinasi Ekstrak Daun Binahong-Rimpang Temu Lawak

2019 ◽  
pp. 89-96
Author(s):  
Nanang Yunarto ◽  
Nurul Aini ◽  
Intan Sari Oktoberia ◽  
Indah Sulistyowati ◽  
Arifayu Addiena Kurniatri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Hmg Coa Reductase ◽  
Activity Test ◽  
Dpph Method ◽  
Curcuma Xanthorrhiza ◽  
Ethanol Extracts ◽  
Coa Reductase ◽  
Better Than

TThe prevalence of dyslipidemia in Indonesia continues to increase. Anredera cordifolia and Curcuma xanthorrhiza are empirically used in the community to overcome dyslipidemia disorders. Flavonoids as the main compounds in A. cordifolia and curcumin in C. xanthorrhiza have potential as antioxidant activity. This study aims to determine the in vitro mechanism of A. cordifolia and C. xanthorrhiza to reduce cholesterol associated with antioxidant activity. The testing was carried out on a combination of ethanol extracts of A. cordifolia leaves with C. xanthorrhiza rhizomes (1:1) and their single extract. The antioxidant activity test was conducted using DPPH method by making serial dilution of samples and ascorbic acid with adding DPPH. The inhibitory activity of HMG CoA and lipase enzymes was carried out enzymatically using ELISA as well as simvastatin as the comparison. The results showed that the highest antioxidant activity is in C. xanthorrhiza extract samples IC50 16.21 ± 1.74 µg/mL, but was less potential compared to ascorbic acid IC50 7,63±0,85 µg/mL. The most potential inhibition of HMG CoA and lipase is in C. xanthorrhiza samples IC50 8.35 ± 0.02 and 22.35 ± 1.26 µg/mL. The combination of A. cordifolia and C. xanthorrhiza (1: 1) is better than A. cordifolia extract but lower than C. xanthorrhiza extract in antioxidant activity as well as the inhibition of HMG CoA reductase and lipase. The result showed a correlation that the higher antioxidant activity, the higher inhibitory of HMG CoA reductase and lipase respectively.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
Julia Elsa Lakoro ◽  
Max R. J. Runtuwene ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Compounds ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of  53,34 mg / L.  Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH  ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Deepak Jain ◽  
Meenakshi Jain ◽  
Anurekha Jain
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Test Protocol ◽  
Rauwolfia Serpentina ◽  
Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals HMG-CoA reductase and lipase enzyme inhibition from combination of Gynura procumbens and Curcuma xanthorrhiza aqueous extract

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 386-392
Author(s):  
E. Setyowati ◽  
Z. Ikawati ◽  
T. Hertiani ◽  
I.D.P. Pramantara
Keyword(s):  
Aqueous Extract ◽  
Pancreatic Lipase ◽  
Positive Control ◽  
Hmg Coa Reductase ◽  
Lipase Enzyme ◽  
Ic50 Value ◽  
Curcuma Xanthorrhiza ◽  
Coa Reductase ◽  
Potent Inhibitory Activity

Lifestyle habits related to consuming excessive fatty foods lead to high levels of lipids in the blood. Some drugs are used to reduce lipids by inhibiting the action of the enzyme HMG-CoA reductase and the pancreatic lipase. The Curcuma xanthorrhiza rhizome and Gynura procumbens leaves were empirically used to reduce cholesterol and lipid levels. This study used the combination of aqueous extract of G. procumbens and C. xanthorrhiza rhizomes (4:1) and their single forms as herbs tested. Furthermore, this study aimed to determine the activity of the tested herbs in inhibiting the enzymatic actions of HMG-CoA reductase and pancreatic lipase in vitro, compared with quercetin, curcumin, and positive control. The results showed that the aqueous extract of C. xanthorrhiza with an IC50 value of 127.54 ppm has the highest activity in inhibiting the enzyme HMG-CoA reductase compared to the aqueous extract of G. procumbens and their combination. In the inhibition of lipase enzyme, G. procumbens aqueous extract had the most potent inhibitory activity compared to C. xanthorrhiza and their combination with an IC50 value of 100.08 ppm.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN GULMA SIAM (Chromoleana odorata L.) DENGAN METODE 1,1-DIFENIL-2-PIKRILHIDRAZIL

2017 ◽  
Vol 1 (2) ◽  
pp. 131-142
Author(s):  
Ajmi Saputra ◽  
Abdul Gani ◽  
Erlidawati Erlidawati
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethanol Extract ◽  
Complete Separation ◽  
Phytochemical Screening ◽  
Inhibitor Concentration ◽  
Methanol Fraction ◽  
Activity Test ◽  
Dpph Method ◽  
Function Group

Abstrak. Telah dilakukan penelitian uji aktivitas antioksidan daun gulma siam (Chromoleana odorata L.) dengan metode 1,1-difenil-2-pikrilhidrazil (DPPH). Tujuan penelitian ini untuk mengetahui kandungan senyawa metabolit sekunder, golongan flavonoid dan aktivitas antioksidan yang terdapat dalam daun gulma siam. Sebanyak 315 g daun gulma siam kering yang sudah dihaluskan, dimaserasi dengan etanol 95% diperoleh 100 g ekstrak kental etanol. Sebanyak 70 g ekstrak kental etanol difraksinasi dengan pelarut metanol dan n-heksana menghasilkan fraksi kental metanol 30,72 g dan n-heksana 14,62 g. Hasil skrining fitokimia ekstrak etanol menunjukkan positif mengandung senyawa alkaloid, flavonoid, saponin, tanin dan steroid. Fraksi metanol positif mengandung senyawa alkaloid, flavonoid, saponin, dan tanin. Fraksi metanol dilakukan isolasi dengan metode kromatografi kolom menggunakan eluen n-heksana : etil asetat : diklorometana (1:1:1) sebagai fase gerak pertama, kemudian ditingkatkan gradien kepolarannya untuk menghasilkan pemisahan secara sempurna. Hasil isolasi diperoleh 8 fraksi (A, B, C, D, E, F, G, H) dan hasil analisis secara KLT menunjukkan fraksi A dan B positif mengandung senyawa flavonoid. Senyawa flavonoid yang terkandung dalam fraksi A diperkirakan golongan isoflavon dan fraksi B diperkirakan golongan isoflavon, flavon, flavonol dan kalkon. Analisis FTIR menunjukkan fraksi A dan B mengandung gugus fungsi N-H, O-H (asam karboksilat), C=O (aldehid dan ester), C=C, C-H, C-X (flourida), C-O dan C-N. Hasil pengujian aktivitas antioksidan menggunakan metode DPPH, diperoleh Inhibitor concentrasi (IC50) untuk ekstrak etanol 15,5067 ppm, fraksi metanol 9,5671 ppm, fraksi A 82,7808 ppm dan fraksi B 16,2336 ppm, sedangkan untuk pembanding (asam askorbat) 0,8913 ppm. Dengan demikian, dapat disimpulkan aktivitas antioksidan yang paling kuat terdapat pada  fraksi metanol.Kata kunci: daun gulma siam, Chromoleana odorata L., flavonoid, DPPH. Abstract. The antioxidant activity of siam weed leaf (Chromoleana odorata L.) has been done with 1,1-diphenyl-2-picrilhidrazil (DPPH) method. This study is intended to know the the content of secondary metabolite compounds, the group of flavonoid and antioxidant activity that found in siam weed leaf. The 315 g dry siam weed leaf that was refined, macerated with ethanol 96% produced 100 g of ethanol viscous extract. The 70 of ethanol viscous extract was fractionated with methanol and n-hexane solvent produced 30,72 g of methanol viscous fraction and 14,62 g n-hexane. The results of phytochemical screening of ethanol extract showed positive that contained compound of alkaloids, flavonoids, saponins, tannins and steroids. Methanol fraction showed positive that contained compound of alkaloids, flavonoids, saponins and tannins. The isolation in methanol fraction was made by coloumn chomatography method using eluent n-hexene : acetate ethyl : dichloromethane (1:1:1) as first mation phase. Then the polarity gradient was increased to produce complete separation. The result of isolation produced 8 fractions (A, B, C, D, E, F, G, H) and the analysis results with TLC showed fraction of A and B was positive contain flavonoids compound. Flaconoids compound that was contained in fraction A was estimated isoflvone group and fraction B was estimated group os isoflavone, flavone, flavonol and chalcone. Analysis FTIR showed that the fraction of A and B containing function group N-H, O-H (carboxylic acid), C=O (aldehyde and ester), C=C, C-H, C-X (flouride), C-O and  C-N. The result of antioxidant activity test used DPPH method, obtained inhibitor concentration (IC50) for 15,5067 ppm of ethanol extract, 9,5671 of methanol fraction, 82,7808 ppm of fraction A and 16,2336 ppm of fraction B, while for comparison (ascorbic acid) is 0,8913 ppm. This, it can be concluded that strongert antioxidant activity was found in methanol fraction.Keywords: siam weed leaf, Chromoleana odorata L., DPPH.

scholarly journals In vitro antioxidant potential study of some synthetic quinoxalines

2012 ◽  
Vol 38 (2) ◽  
pp. 47-50 ◽  
Author(s):  
MM Hossain ◽  
MM Hossain ◽  
MH Muhib ◽  
MR Mia ◽  
S Kumar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Conventional Heating ◽  
Dpph Radical ◽  
Cyclic Ketones ◽  
Scavenging Effect ◽  
Dpph Method ◽  
Quinoxaline Derivatives ◽  
In Vitro Antioxidant Activity

In continuation of our study the in vitro antioxidant activity of some novel quinoxaline derivatives was investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with respect to ascorbic acid. To determine the antioxidant activity, a number of substituted indoxyls (3A-G), cyclic ketones (2A-G), and quinoxalines (1A-G) were synthesized by both microwave and conventional heating methods. The present findings revealed that some quinoxalines and their precursors (1D, 1F, 1G and 2E) exhibited a marked scavenging effect on DPPH radical. DOI: http://dx.doi.org/10.3329/bmrcb.v38i2.12880 Bangladesh Med Res Counc Bull 2012; 38: 47-50

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN MANGGA KASTURI (Mangifera casturi Kosterm.)

2021 ◽  
Vol 3 (3) ◽  
pp. 162-173
Author(s):  
Dwi Lestari ◽  
Muthia Dwi MA ◽  
Jati Pratiwi ◽  
Lidya Handoko Saputri
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethanol Extracts

Mangga Kasturi (Mangifera casturi Kosterm.) is one of the plants in Indonesia that has potential as an antioxidant. The purpose of this study was to determine the IC50 value and antioxidant potential of the ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.). The ethanol extract of mangga kasturi leaves (Mangifera casturi Kosterm.) was extracted using maceration with 96% ethanol as solvent. The antioxidant activity test was carried out using the DPPH (1,1-Diphenyl-2-Picrylhydrazil) method using a UV-Vis spectrophotometer and vitamin C as a comparison. The results of phytochemical screening tests on ethanol extracts showed positive secondary metabolites such as alkaloids, flavonoids, tannins, and quinones. Testing the ethanol extract of Mangga Kasturi leaves (Mangifera casturi Kosterm.) with the DPPH method showed that the ethanol extract had an antioxidant activity with an IC50 value of 83.61 ppm in the strong category of antioxidant potential.

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