EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

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Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

Jurnal Aplikasi Teknologi Pangan ◽

10.17728/jatp.4201 ◽

2019 ◽

Vol 8 (3) ◽

pp. 86

Author(s):

Hasim Hasim ◽

Yupi Yulianita Arifin ◽

Dimas Andrianto ◽

Didah Nur Faridah

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Ethanol Extract ◽

Total Phenolic ◽

Phytochemical Screening ◽

Phytochemical Compounds ◽

Anti Inflammatory ◽

Ethanol Extracts ◽

Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

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Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v18i01.12161 ◽

2021 ◽

Vol 18 (1) ◽

pp. 60-67

Author(s):

Naelaz Zukhruf Wakhidatul Kiromah ◽

Sadam Husein ◽

Titi Pudji Rahayu

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Leaf Extract ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Synthetic Antioxidant ◽

Activity Test ◽

Impaired Liver ◽

Ic50 Value ◽

Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

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Potential of Miana Leaf Extract as Expectorant (Profile Place of Growing, Antioxidant, Sputum Contaminants, Antibacterial, MIC, MKC Expectorant)

Majalah Obat Tradisional ◽

10.22146/mot.52500 ◽

2020 ◽

Vol 25 (2) ◽

pp. 94

Author(s):

Sesilia Rante Pakadang ◽

Santi Sinala ◽

Alfrida Monica Salasa ◽

St Ratnah ◽

Sisilia Teresia Rosmala Dewi ◽

...

Keyword(s):

Antioxidant Activity ◽

Effective Dose ◽

Leaf Extract ◽

Sputum Sample ◽

Dilution Method ◽

Klebsiella Pneumonia ◽

Enterobacter Agglomerans ◽

Dpph Method

Research has been conducted on the treatment of phlegm cough with miana leaf extract in vitro (effective dose of miana leaf extract as an expectorant and antibacterial agent causing cough with phlegm). The study aims to compare the antioxidant activity of miana leaves from 3 locations where it grows, determine the types of contaminant bacteria in the sputum of cough sufferers, determine the minimum value of inhibitor concentration (MIC) and MKC of miana leaves against the test bacteria causing cough with phlegm, determine the effective dose of miana leaves that can used as a reference for coughing up phlegm and proving the potential of miana leaves as a sputum thinner. Miana leaf extraction is done by the juicer method. Antioxidant activity testing uses the DPPH method. Determination of test bacteria is done by isolating and identifying contaminant bacteria in the sputum sample of cough with phlegm. Testing the effectiveness of miana leaves against test bacteria is determined by the liquid dilution method. Expectorant activity testing was determined by measuring the viscosity of mucus viscosity of cow intestine treated with miana leaf extract. The results found that antioxidant activity was influenced by the location where miana leaves grew with an antioxidant potential of IC72 0.072 mg/ml - 0.76 mg/ml. Contaminant bacteria from sputum samples of cough patients are Streptococcus pneumonia, Klebsiella pneumonia, Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter agglomerans. MIC values for contaminant bacteria are 0.1% - 0.75% and MKC values are found between 0.25% - 1.75%. miana leaf extract has the potential as a sputum thinner at a concentration of 0.01% - 0.1%. The recommended dose of miana leaf extract as a cough with phlegm is 1.75% w/v.

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In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i430185 ◽

2020 ◽

pp. 48-60

Author(s):

Udedi Stanley Chidi ◽

Ani Onuabuchi Nnenna ◽

Asogwa Kingsley Kelechi ◽

Maduji Fitzcharles Chijindu ◽

Okafor Clinton Nebolisa

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Superoxide Dismutase ◽

Leaf Extract ◽

Dpph Radical ◽

Β Carotene ◽

In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

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Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.)

Jurnal Penelitian Kesehatan SUARA FORIKES (Journal of Health Research Forikes Voice) ◽

10.33846/sf11110 ◽

2019 ◽

Vol 11 (1) ◽

pp. 49

Author(s):

Tinta Julianawati ◽

Hendy Hendarto ◽

Widjiati Widjiati

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Herbal Medicine ◽

Leaf Extract ◽

Total Flavonoid ◽

Peroxyl Radicals ◽

Chemical Components ◽

Dpph Method ◽

Moringa Leaf Extract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants

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QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.236 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Deepak Jain ◽

Meenakshi Jain ◽

Anurekha Jain

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Alcoholic Extract ◽

Test Protocol ◽

Rauwolfia Serpentina ◽

Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

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A Comparative Study of Antioxidant Activity between Ethanolic and Aqueous Extract of Cardiospermum halicacabum

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00820 ◽

2021 ◽

pp. 4715-4718

Author(s):

Kumudhavalli M.V. ◽

Nandhinipriya V.

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Ascorbic Acid ◽

Hydroxyl Radical ◽

Aqueous Extract ◽

Research Work ◽

Assay Method ◽

Vitro Assay ◽

Aqueous Solvent ◽

Dpph Method

Cardiospermum halicacabum is conventionally used in the treatment of stiffness of limbs, chronic bronchitis, and snakebites. The whole plant of Cardiospermum halicacabum has been extracted using ethanol and aqueous solvent and estimation of their antioxidant activity done for predicted current research work. Both ethanol and aqueous extracts illustrate important antioxidant activity in the DPPH method, nitric oxide method, and hydroxyl radical-induced In-vitro assay method. The DPPH radical inhibition (%) was 53.13, 57.13, and 79.12 for EECH, AECH, and Standard ascorbic acid respectively. The Cardiospermum halicacabum extracts (EECH and AECH) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of nitric oxide inhibition, respectively. The EECH and AECH extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/HO system, when compared to that of ascorbic acid. The plant Cardiospermum halicacabum has been used to treat different ailments. The present study clearly showed that both the extracts viz. EECH and AECH of the plant possess significant antioxidant activity. Moreover, ethanolic extract was proved as more potent candidature as compared to aqueous extract. The molecular level mechanism is the future interest of the researchers to prove the traditional use of the plant.

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In vitro antioxidant potency of some smaller chain glycopeptides with the prediction of IC50 values

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v4i2.6298 ◽

2016 ◽

Vol 4 (2) ◽

pp. 127

Author(s):

Kandasamy Nagarajan ◽

Parul Grover ◽

Roma Ghai ◽

Ayushi Teharia ◽

Sanjeev Chauhan ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Ascorbic Acid ◽

In Vitro Assay ◽

Scavenging Activity ◽

Vitro Assay ◽

Standard Drug ◽

Ic50 Value ◽

Ic50 Values ◽

Antioxidant Potency

Hydrogen peroxide, 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and Phosphomolybdenum in-vitro assay was employed to determine the antioxidant potency of glycopeptides RN Mannose, RK starch, RNRN Mannose and RHRCR Starch using ascorbic acid as the standard drug. The percentage scavenging activity of the glycopeptides were determined at different concentrations and the IC50 value of the test compounds were subsequently compared with that of ascorbic acid. RN Mannose was found to be most potent antioxidant compound. Also, Swiss dock study was performed with three glycopeptides, viz., RHRCR Mannose, RN Mannose and RNRN Mannose.Among these, RHRCR Mannose was found to have the best affinity for the receptor with stearic energy -0.2306kcal/mol.

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