scholarly journals Multilobular Tumor of Bone in a Dog

2019 ◽  
Vol 47 ◽  
Author(s):  
Aline De Biasi Bassani Gonçalves ◽  
Paulo Henrique Leal Bertolo ◽  
Maria Eduarda Bastos Andrade Moutinho da Conceição ◽  
Geórgia Modé Magalhães ◽  
Marcio De Barros Bandarra ◽  
...  
Keyword(s):  
Bone Matrix ◽  
Clinical Signs ◽  
Bone Sarcoma ◽  
Metastatic Potential ◽  
Bone Neoplasm ◽  
Skull Bone ◽  
High Power Field ◽  
Metastatic Sites ◽  
Uncommon Neoplasm ◽  
Histopathologic Exam

Background: Multilobular tumor of bone (MTB) is a primary bone neoplasm, uncommon in dogs. This tumor was called of many names for a long time, as: chondroma rodens, multilobular osteochondrosarcoma, multilobular osteoma, multilobular chondroma, calcificating aponeurotic fibroma, although MTB was preferred chosen, because these other name could be correlated with humans’ tumors. This tumor is observed specially in skull bone, although it was reported in zygomatic arc, hard palate, axilla, spine and penis. Mostly happen in big breeds dogs, and middle to old age patients. Clinical signs depending of region and how aggressive the tumor is, usually are related to compression of any structure. The growth of MTB is frequently slow and progressive, locally invasive, occurring relapse after surgical revomal, although the tumor has low to moderate metastatic potential. The aim of this study is to report a case of MTB in a female dog and describe anatomopathological changes.Case: A female dog, mixed breed, 13 year-old, of middleweight was admitted in Pathology Department of College of Agricultural Sciences and Veterinary Medicine (FCAV-Unesp), Campus of Jaboticabal - SP, to be undergone to necropsy. The patient have never shown any epileptic crisis or neurologic signs. In macroscopic examination was found a mass in skull, which invaded the orbit and frontal sinus, but it was not invading brain cavity. The neoplasm had and irregular surface, firm consistent, color was white mixed to red areas, after cut it was granular and rough, and had some point mineralized areas. The lobs of lung had much firm masses, colored gray to white. In cytology it was observed fusiform to polyhedric isolated mesenchymal cells, moderated pleomorphic, basophilic cytoplasm, thin granulated nuclear chromatin, and visible nucleoli that was involved by eosinophilic extracellular matrix. The proposed diagnostic was bone sarcoma. Histopathological assessment showed mesenchymal neoplastic proliferation, and multilobular characteristic, the lobules had different sizes and was well organized, and they were separated by thin conjunctive septs. In the center of lobules, there was an “island” with mineralized or chondroitin bone matrix, in some of these islands there were osteoclast. The cells had moderated pleomorphism and low mitotic activity (three mitotic cells in ten high-power field). At least, it showed big necrosis areas and invasion of near tissue. In lung was observed metastatic areas, which had same histopathologic way of primary neoplasm in skull. That way, the histopathologic exam was similar to Multilobular tumor of bone grade II.Discussion: The MTB is an uncommon neoplasm, which assaults mostly skull bone in large breeds dogs and middle age of eight years old. This case accord to literature about breed size, age and local of tumor.  The clinical signs in this dog was related to region what it was growing and near structure compression. Besides that, the patient had lung metastasis, that is the principal metastatic sites according to literature. The Histopathologic exam showed the same characters of MTB, which was considered grade II according to literature. Despite MTB is an uncommon neoplasm in clinic of dogs and cats, the epidemiological knowns as age, breed and localization, anatomopathological changes, and histopathological exam allows to have a diagnosis, showing the importance of including this neoplasm in differential diagnosis of bone tumor in dogs. 

scholarly journals True Infection by Capillaria hepatica in a Dog

2021 ◽  
Vol 49 ◽  
Author(s):  
Vanessa Caparelli de Oliveira ◽  
Marina Cazarini Madeira ◽  
Trayse Graneli Soares ◽  
Isabel Rodrigues Rosado ◽  
Ian Martin ◽  
...  
Keyword(s):  
Complete Blood Count ◽  
Present Report ◽  
Abdominal Cavity ◽  
Correct Diagnosis ◽  
Clinical Signs ◽  
Specific Treatment ◽  
Domestic Animals ◽  
Free Fluid ◽  
Capillaria Hepatica ◽  
Histopathologic Exam

Background: Capillaria hepatica is a nematode, zoonotic, with worldwide distribution. The main hosts are rodents, nevertheless other mammals can be affected. Although the parasite has high affinity for the liver, it rarely causes a hepatic disease in domestic animals and humans. The diagnosis is difficult and usually a biopsy is required. The treatment is difficult and is based in anti-helminthic and corticoid, but prevention is the best strategy against the disease. The aim of the present report is to describe a case of hepatic capillariosis in a dog approaching the clinical signs, diagnosis and therapeutic. Case: A 12-year-old Brazilian Terrier bitch, with a history of visit to the farm and regular hunting of rats, frogs, birds and other wild animals, was attended with hyporexia and apathy. At the physical exam the dog presented elevation of rectal temperature, intense jaundice and abdominal pain. In the biochemical exams was noticed a slight increase in globulins and a sharp increase in alkaline phosphatase (AP), total, direct and indirect bilirubin, suggesting a liver injury. In the ultrasonographic exam, hepatomegaly with dispersed hyperechoic areas were observed, suggesting hepatic steatosis. The patient was treated with ursodesoxicolic acid and S-adenosil metionin for 30 consecutive days, showing a clinic improvement. Two months after the end of the treatment the animal worsened, showing jaundice, ascites, motor incoordination, weakness, difficulty in food and water ingestion and changes in the mental state. In the complete blood count was observed a macrocytic hypochromic regenerative anemia, leukocytosis with neutrophilia and monocytosis and thrombocytopenia. In biochemical exams was detected decrease in creatinine and albumin and increase in alanine aminotransferase and AP, suggesting hepatopathy by biliary obstruction. There were performed exams for leishmania and ehrlichiosis that tested negative. In the ultrasonographic exam it was noticed that the liver had the same alterations and the presence of free fluid in the abdominal cavity. Due to the deterioration of the clinical picture a blood transfusion was necessary, however the patient worsened and presented respiratory difficulty for a bilateral pleural effusion. Then, the fluid was drained and a treatment with S-adenosil metionin, silymarin, ursodesoxicolic acid, doxycycline and prednisolone was started. After 17 days, a hepatic biopsy was performed, but the patient died at the surgery desk. Samples from the liver were collected for histopathologic exam. The diagnosis was confirmed in hepatic capillariosis and periportal chronic hepatitis, with the visualization of numerous parasites structures with bioperculated barrel shape. Discussion: Due to the nonspecific clinical alterations observed in the patient and the low prevalence of C. hepatica in domestic animals, there was a difficulty in the diagnosis which lead to a symptomatic, nonspecific and inefficient treatment that culminate with the death of the patient. The histopathologic exam of the liver is the best manner to find the correct diagnosis of C. hepatica infection, once the parasites eggs remains in the liver parenchyma wrapped by fibrosis and aren´t eliminate in the feces. The eggs elimination in the environment just occur after the death of the animal. Although there didn't exist a consensus about hepatic capillariosis treatment yet, if the diagnosis were done earlier, a specific treatment with better chances of a good result could be performed. We concluded that hepatic capillariosis should be included in differential diagnosis for patients with hepatic syndrome, mainly if the animal had a hunting habit and if it had access to surroundings with high rats’ infestation.

scholarly journals Localisation Buccale Secondaire D’un Carcinome Renal

2022 ◽  
Vol 5 (1) ◽  
pp. 01-03
Author(s):  
Ikrame Boumendil
Keyword(s):  
Renal Cell Carcinoma ◽  
Renal Cell ◽  
Metastatic Potential ◽  
Metastatic Lesion ◽  
Renal Cell Carcinomas ◽  
Initial Manifestation ◽  
Facial Surgery ◽  
Carcinome Rénal ◽  
Metastatic Sites ◽  
Localization Of Metastases

Renal cell carcinomas are tumors known for their metastatic potential. The lungs, the lymph nodes, the lungs, the spleen, the adrenal gland and the cervix remain the metastatic sites of predisposition. The symptoms of metastatic lesion may be due to the initial manifestation of renal malignancy. We report in this work a buccal localization of metastases from renal cell carcinoma to clear cells in a patient aged 65 years or less in our department of otolaryngology and cervical-facial surgery.

scholarly journals Clinical, pathological and therapeutic findings on lingual rhabdomyosarcoma in a dog - case report

2019 ◽  
Vol 71 (6) ◽  
pp. 1815-1820
Author(s):  
F.G.G. Dias ◽  
J. Santilli ◽  
G.M. Magalhães ◽  
S.G. Calazans ◽  
L.F. Pereira ◽  
...  
Keyword(s):  
Case Report ◽  
Clinical Stage ◽  
Companion Animals ◽  
Safety Margin ◽  
Clinical Signs ◽  
Surgical Excision ◽  
Metastatic Potential ◽  
Histopathological Analysis ◽  
Biological Behaviour ◽  
Uncommon Condition

ABSTRACT The biological behaviour of the tumours vary according to the species in which they occur, its location within the cavity, clinical stage and histopathological nature. Lingual neoplasms are generally uncommon in companion animals. Rhabdomyosarcomas are malignant, solid, aggressive formations with high metastatic potential. The clinical signs are variable and a definitive diagnosis can only be reached through histopathological analysis of biopsy and necropsy specimen. In some cases, immunohistochemical study may be needed to confirm the diagnosis. This paper aims to highlight important points about this uncommon condition in dogs, using a case report of lingual rhabdomyosarcoma, which showed no evidence of metastasis after diagnosis, nor of local recurrence after surgical excision with a wide safety margin. It was concluded that early diagnosis, the correct interpretation of the complementary tests and the appropriate therapeutic approach contributed to improving the quality of life and survival of the patient in question.

scholarly journals Clinical and Laboratory Findings in Dogs Undergoing Adjuvant Chemotherapy with Gemcitabine/Carboplatin Combination for Mammary Neoplasia

2021 ◽  
Vol 11 (2) ◽  
pp. 289-299
Author(s):  
Carolina Bistritschan Israel ◽  
Tábata Maués ◽  
Ana Maria Reis Ferreira ◽  
Maria de Lourdes Gonçalves Ferreira
Keyword(s):  
Clinical Trial ◽  
Adjuvant Chemotherapy ◽  
Adverse Events ◽  
Mammary Cancer ◽  
Clinical Signs ◽  
Metastatic Potential ◽  
Prospective Clinical Trial ◽  
Laboratory Findings ◽  
Chemotherapy Protocol ◽  
Mammary Neoplasia

Adjuvant chemotherapy might be indicated in some canine mammary cancer cases due to metastatic potential. In this regard, studies to determine adverse events following chemotherapy protocols are valuable. The purpose of this prospective clinical trial was to evaluate the safety and tolerability of gemcitabine and carboplatin combination in dogs with malignant mammary tumors. For this prospective clinical trial, 21 female dogs mastectomized due to malignant mammary neoplasia underwent adjuvant chemotherapy with gemcitabine (3 mg/kg, 60-minute IV infusion) and carboplatin (10 mg/kg, 20-minute IV infusion) based protocol every 21 days for three cycles. They were monitored periodically for treatment-related adverse events by clinical and laboratory evaluations. A total of 17 (80.9%) dogs developed leukopenia, 10 (47.6%) neutropenia, and 15 (71.4%) thrombocytopenia at least once along with the three chemotherapy cycles. All these hematologic toxicities were grade 1, 2, or 3. Two (9.5%) animals had evidence of gastrointestinal toxicity; however, clinical signs were mild to moderate (grades 1 and 2). No dog had life-threatening adverse events (grade 4) or even died (grade 5) of treatment-related complications. The adjuvant chemotherapy protocol with gemcitabine and carboplatin was well-tolerated and safe in female dogs for mammary cancer treatment with self-limiting hematological and gastrointestinal adverse events.

161 COMPARISON OF DIFFERENT DIAGNOSTIC METHODS IN EQUINE ENDOMETRITIS

2015 ◽  
Vol 27 (1) ◽  
pp. 171
Author(s):  
T. Chenier ◽  
M. Diel de Amorim ◽  
R. A. Foster ◽  
A. Hill ◽  
T. Hayes ◽  
...  
Keyword(s):  
Gold Standard ◽  
Laboratory Data ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Endometrial Biopsy ◽  
Diagnostic Methods ◽  
High Power Field ◽  
Practice Population ◽  
Uterine Fluid ◽  
Endometrial Cytology

Prolonged endometritis is the most common cause of infertility in mares causing great economic impact. Many mares fail to be diagnosed with endometritis despite the availability of different diagnostic tests. Therefore, the purpose of this study was to compare endometrial swab, low-volume lavage (LVL) and endometrial biopsy as diagnostic methods for endometritis and to report the prevalence of this disease in a referral practice population. Fifty-one mares presenting for routine breeding or infertility work-up were examined by transrectal ultrasonography, before collecting samples for endometrial culture and cytology. Seven of the 51 mares had all the tests except endometrial biopsy. A mare was classified positive for endometritis if she demonstrated two or more of the following 5 criteria on a checklist (new gold standard; NGS): (1) abnormal clinical findings (any of uterine fluid on ultrasound, or excessive oedema for follicular size, or history of subfertility); (2) abnormal gross character of the LVL fluid: (cloudy, discolored, debris) before centrifugation; (3) positive endometrial cytology (≥1 neutrophil per high power field, or ≥1% (1 : 100) neutrophil to epithelial cell ratio on cytology); (4) bacterial growth on culture of the LVL pellet; and (5) histological evidence of inflammation (acute, chronic, and mixed) detected on endometrial biopsy. Data were analysed via kappa coefficient (k) and frequencies were calculated for sensitivity and positive predictive value (PPV) with biopsy being the gold standard and compared to the NGS. Endometritis was diagnosed in 35/44 (79.5%) mares by biopsy (5/35 had acute endometritis, 12/35 had chronic; 18/35 had a combination of acute and chronic endometritis). Based on the endometritis criteria (2/5 items on the checklist), 33/51 (64.7%) mares were diagnosed to have endometritis. All 11 of the barren mares were diagnosed by the checklist, while two of these 11 mares had no evidence of endometritis by biopsy, but had clinical signs or cloudy efflux. The character of the endometrial flush was 45% sensitive (k = 0.046), while culture was 22% sensitive, when compared to endometrial biopsy. When each criterion for endometritis was compared against the NGS, endometrial biopsy was the most sensitive diagnostic method (sensitivity = 86%). Abnormal clinical findings showed moderate agreement with the NGS (k = 0.4138), with a sensitivity of 62% and P = 0.0019. Positive endometrial cytology showed similar agreement (k = 0.3761), and sensitivity (sensitivity = 64%, and P = 0.0069). These studies have also shown the importance of using laboratory data in light of clinical findings, since they have shown that no test by itself is sensitive enough to diagnose a mare with subclinical endometritis, and that this disease might be under diagnosed. Since this study was performed in a referral hospital, there may have been a higher prevalence of endometritis than found in general clinical practice. An endometritis checklist could be used in cases where endometrial biopsies are not readily available.

Clinicopathological and ultrasonographic features of cats with eosinophilic enteritis

2014 ◽  
Vol 16 (12) ◽  
pp. 950-956 ◽  
Author(s):  
Samuel Tucker ◽  
Dominique G Penninck ◽  
John H Keating ◽  
Cynthia RL Webster
Keyword(s):  
Clinical Signs ◽  
Clinical Pathology ◽  
Abdominal Ultrasound ◽  
Clinicopathological Feature ◽  
Peripheral Eosinophilia ◽  
High Power Field ◽  
Tissue Eosinophilia ◽  
Ultrasound Findings ◽  
Inflammatory Bowel ◽  
Jejunal Wall

Eosinophilic enteritis (EE) in cats is poorly characterized. The aim of the current study was to retrospectively evaluate the clinical and ultrasonographic findings in cats with histologic evidence of eosinophilic inflammation on gastrointestinal biopsy. Twenty-five cats with tissue eosinophilia on surgical (10) or endoscopic (15) biopsy of the gastrointestinal tract, having an abdominal ultrasound performed within 48 h of biopsy acquisition, were enrolled. History, clinical presentation, clinical pathology and abdominal ultrasound findings were reviewed. Intestinal biopsies were evaluated by a single pathologist and separated into two groups based on the degree of eosinophilic infiltrate: mild (<10 eosinophils/high-power field [HPF], 11/25 cats), or moderate/marked (>10 eosinophils/HPF, 14/25 cats). The former were considered primary lymphoplasmacytic or lymphocytic inflammatory bowel disease (LPE) with subtle eosinophilic infiltrates, and the latter to have EE. Signalment, history and clinical signs were similar in all cats. Only cats with EE (6/14) had palpably thickened intestines. The only distinguishing clinicopathological feature of cats with EE was the presence of peripheral eosinophilia (6/14). On ultrasound, when compared with cats with LPE, cats with EE had a greater mean jejunal wall thickness (3.34 mm ± 0.72 mm vs 4.07 mm ± 0.58 mm, respectively) and an increased incidence of thickening of the muscularis layer (1/11 and 11/14, respectively). In conclusion, ultrasonographic evidence of a prominent intestinal muscularis layer, palpably thickened intestines and peripheral eosinophilia can serve as biomarkers for the presence of EE in cats with chronic intestinal signs.

Identification and Characterization of Hematopoietic Stem (HSC)/Progenitor Cells in Human Solid Tumors

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4785-4785
Author(s):  
Roland C Leung ◽  
David C Dorn ◽  
Robert J Downey ◽  
Douglas A Levine ◽  
Malcolm A.S. Moore
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Solid Tumors ◽  
Primary Tumor ◽  
Colony Formation ◽  
Lung Tumor ◽  
Metastatic Potential ◽  
Alternative Source ◽  
Hematopoietic Stem ◽  
Metastatic Sites

Abstract It has recently been shown that a solid tumor recruits cells to the tumor mass, which facilitate vascularization, suppress immune responses, aid its growth, and influence metastatic potential. These recruited cells include mesenchymal stem cells, endothelial progenitor cells and tumor infiltrating leukocytes. By performing multiparameter FACS analysis on primary lung carcinoma and pleural effusion material, we demonstrate for the first time the consistent presence of CD45+/CD34+ cells (mean 1.6% of total CD45+cells range 0.24–13.2% n=12). In primary tumor samples, CD45+ lymphohematopoietic cells were purified by the MACS system and examined for progenitor colony formation in methycellulose. Erythroid and myeloid colony formation was observed in the CD45+ selected subfraction. We assayed for HSC activity by cobblestone formation (CAFC) on MS-5 stroma. Week 2 and week 5 CAFC potential was present in CD45+ purified ovarian cancer ascites. In non-irradiated NOD/SCID IL2 gamma null mice injected subcutaneously with dissociated and unfractionated lung tumor and ovarian peritoneal ascites, small numbers of human CD45+ cells were recovered from the bone marrow after 2–3 months which gave rise to hematopoietic colonies. Human CD45+ cells also persisted within the retransplanted subcutaneous tumors. In conclusion, we report the recruitment of HSCs and progenitors to primary tumor sites. The differentiation and self-renewal potential of these HSCs indicate a potential alternative source of tumor-infiltrating leukocytes as opposed to or in addition to recruitment of mature cells from the circulation. The role of solid tumors in mobilizing HSCs and progenitors from the bone marrow and their selective chemotaxis to the primary and metastatic sites require further investigation. Targeting this novel tumor-HSC interaction may offer new therapeutic opportunities for controlling tumor growth and metastasis.

scholarly journals Metastatic Pelvic Osteosarcoma in a Dog

2021 ◽  
Vol 49 ◽  
Author(s):  
Felipe Martins Pastor ◽  
Pâmella De Cássia Pimenta Barbosa ◽  
Lígia Assunção Oliveira ◽  
Heloísa Cristina Teixeira de Carvalho ◽  
Gabriel Henrique Guimarães ◽  
...  
Keyword(s):  
Clinical Care ◽  
Metastatic Potential ◽  
Axial Skeleton ◽  
Bone Neoplasm ◽  
Histological Type ◽  
Malignant Neoplasms ◽  
Anatomical Location ◽  
Appendicular Skeleton ◽  
Undifferentiated Sarcoma ◽  
Canine Osteosarcoma

Background: Osteosarcomas are malignant neoplasms of bone tissue, with a high prevalence in dogs, especially in large and giant breeds. More commonly, such alterations affect the appendicular skeleton and, to a lesser extent, the axial skeleton. In order to obtain an accurate diagnosis, it is necessary to combine cytological and histopathological findings with clinical parameters, imaging exams and macroscopic findings. In the present study, we report a rare case of combined-type pelvic osteosarcoma with pulmonary metastasis in a dog.Case: A 5-year-old intact large male dog of mixed breed, was submitted to clinical care because of an increase in volume of the left perineal region.  The cytological evaluation, performed without imaging exams, indicated that it was an undifferentiated sarcoma. An incisional biopsy defined the diagnosis as telangiectatic osteosarcoma, and with progressive clinical worsening, the patient died. Necroscopic examination revealed multiple nodules in the lungs and an irregular mass with a hard to friable consistency. The mass was intensely vascularised and extended craniodorsally from the left ischial tuberosity to the base of the renal fossa. Microscopically, the neoplasm was diagnosed as combined osteosarcoma, consisting of the osteoblastic, chondroblastic, and telangiectatic subtypes. Metastases with a predominance of the chondroblastic subtype were observed in the lungs.Discussion: This is the first report of combined-type canine osteosarcoma in the ischium. The case reported here is unusual, as there are few reports of canine osteosarcoma in the pelvic bones, and there is no concrete information regarding its histological appearance. Osteosarcoma is the most common bone neoplasm in dogs, representing up to 80% of the tumours found in such organs. In the present case, the dog was a large young adult with a higher probability of neoplasm development. A cytopathological examination is a diagnostic method with good sensitivity and specificity that can confirm osteosarcomas. However, in this case, the cytological diagnosis, performed without the information from the imaging exam, indicated that it was an undifferentiated sarcoma, given the impossibility of the architectural assessment of the lesion. Biopsy samples sent for histology may not be representative of the entire tumour, leading to misclassification of the histological type. Therefore, the evaluation of fragments from various sites of the lesions is recommended. Regarding the morphology of osteosarcomas, such neoplasms have the osteoblastic, chondroblastic, fibroblastic, telangiectatic, large cell, and poorly differentiated subtypes. With regard to tumours located in the axial skeleton, no studies have assessed the predominance of a particular morphological type, as well as the incidence of combined-type masses in dogs in this particular location. Such neoplasms are locally aggressive and have a high metastatic potential, with the lungs being the main location for implantation of neoplastic cells. There is no proven evidence of the correlation between morphological presentations and the presence of metastases from osteosarcomas in dogs. The histological type is not a predictive factor for the behaviour of the neoplasm. However, the anatomical location is considered as one of the factors with the greatest influence on the prognosis and metastatic potential. Rib masses are associated with a higher rate of metastases compared to others. The definitive diagnosis of osteosarcomas and its correct subclassification are of great importance in the prognosis of affected patients. These require an approach that considers the clinical findings, imaging examinations, and macroscopic and microscopic alterations. Keywords: bone, canine, cytopathology, histopathology, neoplasm.Título: Osteossarcoma pélvico metastático em cão Descritores: canino, citopatologia, histopatologia, neoplasia, osso.

scholarly journals An unexpected diagnosis of breast malignancy

2017 ◽  
Vol 99 (6) ◽  
pp. e180-e182
Author(s):  
T Richardson ◽  
F Cottier
Keyword(s):  
Breast Cancer ◽  
Survival Rate ◽  
Inflammatory Breast Cancer ◽  
Hodgkin’S Lymphoma ◽  
Clinical Signs ◽  
Metastatic Potential ◽  
Hodgkin's Lymphoma ◽  
Rapid Progression ◽  
Non Hodgkin’S Lymphoma ◽  
Non Hodgkin's Lymphoma

Inflammatory breast cancer constitutes 5% of all breast cancer diagnoses. Diagnosis is based on clinical signs including skin changes, erythema and oedema, together with rapid progression and involvement of more than one-third of the affected breast. It is an aggressive tumour with great metastatic potential, metastases being present in 30% of patients at first presentation. Primary non-Hodgkin’s lymphoma of the breast is rare but is well reported. It accounts for 0.5% of all breast malignancies and 1% of all non-Hodgkin’s diagnoses. Prognosis of primary breast lymphoma varies depending on the stage of disease with stage IE having a 5-year survival rate of 78–83% and stage IIE having a 5-year survival rate of 20–57%. We present a rare case of non-Hodgkin’s lymphoma mimicking an inflammatory breast cancer. The aim of this case report is to highlight an unusual presentation of non-Hodgkin’s lymphoma and the diagnostic difficulties that arise.

Vitamin D Represses the Aggressive Potential of Osteosarcoma

2020 ◽  
Vol 20 ◽  
Author(s):  
Behnoosh Tahbazlahafi ◽  
Malihe Paknejad ◽  
Shahnaz Khaghani ◽  
Sahar Sadegh-Nejadi ◽  
Ehsan Khalili
Keyword(s):  
Vitamin D ◽  
Cancer Progression ◽  
Critical Role ◽  
Scratch Test ◽  
Metastatic Potential ◽  
Bone Neoplasm ◽  
Protein Levels ◽  
Effective Doses ◽  
And Migration

Background: Osteosarcoma (OS) is the basic bone neoplasm with lower survival and poor prognosis. It is distinguished by its offensive nature and metastatic potential. The fundamental death source in OS patients is lung metastasis. In addition, the proliferation and cell migration are thus essential for cancer progression, especially for intrusion and transformation. Several studies have illustrated that 1,25-Dihydroxyvitamin D (1,25(OH)2D) has a critical role in the growth and differentiation of bone. However, knowledge about the outcome of 1,25(OH)2D on the progression and incursion of osteosarcoma cells is minimal. Objective: The present study aimed to analyze the effect of different concentrations of 1,25(OH)2D on the multiplication, progression, and intrusion of OS cells and verify the effective doses of 1,25(OH)2D that can decrease the intensity of the disease and improving the prognosis in OS patients. Methods: Saos-2 cells were treated with 1,25(OH)2D (0, 50, 100, and 200 nM) for 48, 72, and 96 hours. Proliferation, invasion, and migration were determined by MTT assay, Transwell assay, and Scratch test, respectively. The levels of c-Myc and FOXO1 proteins were determined by Western blotting. Results: The proliferation, invasiveness, and migration of Saos-2 cells that were treated with 1,25(OH)2D were significantly decreased compared with untreated cells. Although 1,25(OH)2D notably decreased c-Myc protein levels (after 48 and 72 hours), FOXO1 protein levels have been significantly increased after 48 and 72 hours. 1,25(OH)2D and the vitamin D receptor (VDR) suppress c-Myc function through regulating the c-Myc/MXD1 network and providing thus a molecular basis of 1,25(OH)2D related to the cancer-preventive actions. Conclusion: Based on the present results, 1,25(OH)2D by targeting c-Myc and FOXO1 expression displays anti-invasive, anti-migration and anti-proliferative effects on OS cells in vitro. Our findings suggest that effective doses of the 1,25(OH)2D may reduce of the aggressive potential of OS cell line. However, further investigation and clinical trials are needed.

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