POTENCIAL USO DO SOFOROLIPÍDIO DE STARMERELLA BOMBICOLA COMO INGREDIENTE COADJUVANTE EM PRODUTOS CÁRNEOS EMBUTIDOS

In the last few decades, the increase of ethanol in wine, due to global climate change and consumers’ choice is one of the main concerns in winemaking. One of the most promising approaches in reducing the ethanol content in wine is the use of non-Saccharomyces yeasts in co-fermentation or sequential fermentation with Saccharomyces cerevisiae. In this work, we evaluate the use of Starmerella bombicola and S. cerevisiae in sequential fermentation under aeration condition with the aim of reducing the ethanol content with valuable analytical profile. After a preliminary screening in synthetic grape juice, bench-top fermentation trials were conducted in natural grape juice by evaluating the aeration condition (20 mL/L/min during the first 72 h) on ethanol reduction and on the analytical profile of wines. The results showed that S. bombicola/S. cerevisiae sequential fermentation under aeration condition determined an ethanol reduction of 1.46% (v/v) compared with S. cerevisiae pure fermentation. Aeration condition did not negatively affect the analytical profile of sequential fermentation S. bombicola/S. cerevisiae particularly an overproduction of volatile acidity and ethyl acetate. On the other hand, these conditions strongly improved the production of glycerol and succinic acid that positively affect the structure and body of wine.

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Sophorolipids: improvement of the selective production by Starmerella bombicola through the design of nutritional requirements

Applied Microbiology and Biotechnology ◽

10.1007/s00253-012-4437-x ◽

2012 ◽

Vol 97 (5) ◽

pp. 1875-1887 ◽

Cited By ~ 17

Author(s):

Isabel A. Ribeiro ◽

M. Rosário Bronze ◽

Matilde F. Castro ◽

Maria H. L. Ribeiro

Keyword(s):

Nutritional Requirements ◽

Starmerella Bombicola

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Effect of yeast and essential oil-enriched diets on critical determinants of health and immune function in Africanized Apis mellifera

PeerJ ◽

10.7717/peerj.12164 ◽

2021 ◽

Vol 9 ◽

pp. e12164

Author(s):

César Canché-Collí ◽

Humberto Estrella-Maldonado ◽

Luis A. Medina-Medina ◽

Humberto Moo-Valle ◽

Luz Maria Calvo-Irabien ◽

...

Keyword(s):

Apis Mellifera ◽

Food Intake ◽

Essential Oils ◽

Immune Function ◽

Honey Bees ◽

Fat Body ◽

Control Diet ◽

Starmerella Bombicola ◽

Expression Of Genes ◽

Brewers Yeast

Nutrition is vital for health and immune function in honey bees (Apis mellifera). The effect of diets enriched with bee-associated yeasts and essential oils of Mexican oregano (Lippia graveolens) was tested on survival, food intake, accumulated fat body tissue, and gene expression of vitellogenin (Vg), prophenoloxidase (proPO) and glucose oxidase (GOx) in newly emerged worker bees. The enriched diets were provided to bees under the premise that supplementation with yeasts or essential oils can enhance health variables and the expression of genes related to immune function in worker bees. Based on a standard pollen substitute, used as a control diet, enriched diets were formulated, five with added bee-associated yeasts (Starmerella bombicola, Starmerella etchellsii, Starmerella bombicola 2, Zygosaccharomyces mellis, and the brewers’ yeast Saccharomyces cerevisiae) and three with added essential oils from L. graveolens (carvacrol, thymol, and sesquiterpenes). Groups of bees were fed one of the diets for 9 or 12 days. Survival probability was similar in the yeast and essential oils treatments in relation to the control, but median survival was lower in the carvacrol and sesquiterpenes treatments. Food intake was higher in all the yeast treatments than in the control. Fat body percentage in individual bees was slightly lower in all treatments than in the control, with significant decreases in the thymol and carvacrol treatments. Expression of the genes Vg, proPO, and GOx was minimally affected by the yeast treatments but was adversely affected by the carvacrol and thymol treatments.

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Unraveling the regulation of sophorolipid biosynthesis in Starmerella bombicola

FEMS Yeast Research ◽

10.1093/femsyr/foaa021 ◽

2020 ◽

Vol 20 (3) ◽

Cited By ~ 1

Author(s):

Sofie Lodens ◽

Sophie L K W Roelants ◽

Goedele Luyten ◽

Robin Geys ◽

Pieter Coussement ◽

...

Keyword(s):

Gene Expression ◽

Stationary Phase ◽

Gene Cluster ◽

Biosynthetic Gene Cluster ◽

Exponential Phase ◽

Biosynthetic Gene ◽

Reporter System ◽

Gfp Expression ◽

Qpcr Analysis ◽

Starmerella Bombicola

ABSTRACT Starmerella bombicola very efficiently produces the secondary metabolites sophorolipids (SLs). Their biosynthesis is not-growth associated and highly upregulated in the stationary phase. Despite high industrial and academic interest, the underlying regulation of SL biosynthesis remains unknown. In this paper, potential regulation of SL biosynthesis through the telomere positioning effect (TPE) was investigated, as the SL gene cluster is located adjacent to a telomere. An additional copy of this gene cluster was introduced elsewhere in the genome to investigate if this results in a decoy of regulation. Indeed, for the new strain, the onset of SL production was shifted to the exponential phase. This result was confirmed by RT-qPCR analysis. The TPE effect was further investigated by developing and applying a suitable reporter system for this non-conventional yeast, enabling non-biased comparison of gene expression between the subtelomeric CYP52M1- and the URA3 locus. This was done with a constitutive endogenous promotor (pGAPD) and one of the endogenous promotors of the SL biosynthetic gene cluster (pCYP52M1). A clear positioning effect was observed for both promotors with significantly higher GFP expression levels at the URA3 locus. No clear GFP upregulation was observed in the stationary phase for any of the new strains.

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Elucidation of secondary alcohol metabolism in Starmerella bombicola and contribution of primary alcohol oxidase FAO1

FEMS Yeast Research ◽

10.1093/femsyr/foz012 ◽

2019 ◽

Vol 19 (2) ◽

Author(s):

Fumikazu Takahashi ◽

Kazuaki Igarashi ◽

Yasushi Takimura ◽

Takashi Yamamoto

Keyword(s):

Secondary Alcohol ◽

Primary Alcohol ◽

Alcohol Oxidase ◽

Alcohol Metabolism ◽

Starmerella Bombicola

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The effect of medium composition on the production of sophorolipids and the tensiometric properties by Starmerella bombicola MTCC 1910

Polish Journal of Chemical Technology ◽

10.2478/v10026-010-0011-4 ◽

2010 ◽

Vol 12 (2) ◽

pp. 9-13 ◽

Cited By ~ 11

Author(s):

Nagarajan Vedaraman ◽

Narayana Venkatesh

Keyword(s):

Surface Tension ◽

Yeast Extract ◽

Sunflower Oil ◽

Free Cell ◽

Medium Composition ◽

Candida Bombicola ◽

Standard Medium ◽

Substrate Composition ◽

Starmerella Bombicola ◽

Minimum Surface Tension

The effect of medium composition on the production of sophorolipids and the tensiometric properties by Starmerella bombicola MTCC 1910 Starmerella bombicola a teleomorph of Candida bombicola is capable of producing extracellular secondary metabolites known as sophorolipids. In the present work the performance of Starmerella in producing sophorolipids, with standard medium ingredients glucose, sunflower oil, yeast extract and urea was studied. The quantities of different medium ingredients were optimized to maximize the production of sophorolipids. Variation in tensiometric properties like surface tension and interfacial tension during the incubation period were also reported. The optimized mixed substrate composition was found to be 200 g/l, containing equal amounts of glucose and sunflower oil, 4 g/l of yeast extract and 0.6 g/l of urea. With the optimized substrate composition 38.6 g/l of sophorolipids was obtained. The minimum surface tension produced by the culture free cell broth was 36.2 mN/m. Increasing the temperature from 25°C to 35°C has shown adverse effects on sophorolipids production.

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Exploring the Transportome of the Biosurfactant Producing Yeast Starmerella Bombicola

10.21203/rs.3.rs-778149/v1 ◽

2021 ◽

Author(s):

Silke Claus ◽

Sylwia Jezierska ◽

Liam D.H. Elbourne ◽

Inge N.A. Van Bogaert

Keyword(s):

Computational Analysis ◽

Biosynthetic Gene Cluster ◽

Stages Of Growth ◽

Subcellular Localization Prediction ◽

Hydrophobic Compounds ◽

Starmerella Bombicola ◽

Transporter Family ◽

Main Driver ◽

Localization Prediction ◽

Potential Substrate

Abstract Starmerella bombicola is a non-conventional yeast mainly known for its capacity to produce high amounts of the glycolipids ‘sophorolipids’. Although its product has been used as biological detergent for a couple of decades, the genetics of S. bombicola are still largely unknown. Computational analysis of the yeast’s genome enabled us to identify 254 putative transporter genes that make up the entire transportome. For each of them, a potential substrate was predicted using homology analysis, subcellular localization prediction and RNA sequencing in different stages of growth. One transporter family is of exceptional importance to this yeast: the ATP Binding Cassette (ABC) transporter Superfamily, because it harbors the main driver behind the highly efficient sophorolipid export. Furthermore, members of this superfamily translocate a variety of compounds ranging from antibiotics to hydrophobic molecules. We conducted an analysis of this family by creating deletion mutants to understand their role in the export of hydrophobic compounds, antibiotics and sophorolipids. Doing this, we could experimentally confirm the transporters participating in the efflux of medium chain fatty alcohols, particularly decanol and undecanol, and identify a second sophorolipid transporter that is located outside the sophorolipid biosynthetic gene cluster.

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