scholarly journals Compare the Changes of Groundwater Type and Microbial Community in Four Shallow Aquifers Affected by Seawater Intrusion in the East Coastal of Pearl River Delta

2021 ◽  
Author(s):  
Kang Yang ◽  
Linjie Hu ◽  
Shengcai Huang ◽  
Can Liu ◽  
Liang Feng ◽  
...  
Keyword(s):  
Microbial Community ◽  
Seawater Intrusion ◽  
Shallow Groundwater ◽  
Pearl River ◽  
Groundwater Aquifers ◽  
Class Level ◽  
Α Diversity ◽  
Positive Correlations ◽  
First Time ◽  
Weak Influence

Seawater intrusion has become a serious natural disaster in coastal regions around the world. Four shallow groundwater aquifers of Pearl River were sampled to study the changes of groundwater types and microbial communities caused by seawater intrusion. Seawater intrusion caused significantly increased cations (Na+, K+, Ca2+, Mg2+) and anions (Cl-, SO42-, HCO3-), and the groundwater type turned from HCO3-Ca to Cl•HCO3-Na•Ca and Cl•SO4-Na•Mg. The composition and diversity of groundwater geochemistry changes. Gammaproteobacteria species and Alphaproteobacteria species are dominant species, and the salinity of groundwater is the main environment factor that affect the relative abundance. The α-diversity of microbial community in three types groundwater are significantly different (P = 0.002，R2 = 0.959). The correlation between species in class level with different ions were also observed. Aeromonadales, Vibrionales, Alteromonadales and Oceanospirillales have a significant positive correlation with Cl-, SO42-, Na+ and Mg2+ (P < 0.05) in Cl•SO4-Na•Mg type, while in Cl•HCO3-Na•Ca type groundwater, Vibrionales and Oceanospirillales have positive correlations with Cl-, Na+, Ca2+, but negative correlations with HCO3-. Aeromonadales and Alteromonadales are opposite of this. The results demonstrate that groundwater type is the main factors influence the correlation between species and environments, other geophysical factors have weak influence. This shift of shallow groundwater type and microbial community under seawater intrusion were studied for the first time, it is momentous for forward exploration of groundwater microbial ecology in the coastal area under the background of seawater intrusion.

scholarly journals Microbial Community in High Arsenic Shallow Groundwater Aquifers in Hetao Basin of Inner Mongolia, China

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0125844 ◽  
Author(s):  
Ping Li ◽  
Yanhong Wang ◽  
Xinyue Dai ◽  
Rui Zhang ◽  
Zhou Jiang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Inner Mongolia ◽  
Shallow Groundwater ◽  
Groundwater Aquifers ◽  
High Arsenic

scholarly journals Subsoil microbial community responses to air exposure and legume growth depend on soil properties across different depths

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hongmei Yan ◽  
Fan Yang ◽  
Jiamin Gao ◽  
Ziheng Peng ◽  
Weimin Chen
Keyword(s):  
Microbial Community ◽  
Soil Properties ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Soil Profiles ◽  
Air Exposure ◽  
Structure Variation ◽  
Α Diversity ◽  
Different Depths ◽  
Legume Growth

AbstractAnthropogenic disturbance, such as agricultural and architectural activities, can greatly influence belowground soil microbes, and thus soil formation and nutrient cycling. The objective of this study was to investigate microbial community variation in deep soils affected by strong disturbances. In present study, twelve soil samples were collected from different depths (0–300 cm) and placed onto the surface. We investigated the structure variation of the microbial community down through the soil profiles in response to disturbance originated by legume plants (robinia and clover) cultivation vs. plant-free controls. The high-throughput sequencing of 16S rRNA genes showed that microbial α-diversity decreased with depth, and that growing both plants significantly impacted the diversity in the topsoil. The soil profile was clustered into three layers: I (0–40 cm), II (40–120 cm), and III (120–300 cm); with significantly different taxa found among them. Soil properties explained a large amount of the variation (23.5%) in the microbial community, and distinct factors affected microbial assembly in the different layers, e.g., available potassium in layer I, pH and total nitrogen in layer II, pH and organic matter in layer III. The prediction of metabolic functions and oxygen requirements indicated that the number of aerobic bacteria increased with more air exposure, which may further accelerate the transformation of nitrogen, sulfur, carbon, and pesticides in the soil. The diversity of soil microorganisms followed a depth-decay pattern, but became higher following legume growth and air exposure, with notable abundance variation of several important bacterial species, mainly belonging to Nitrospira, Verrucomicrobia, and Planctomycetes, and soil properties occurring across the soil profiles.

scholarly journals Investigation and analysis of the fungal community structure in moldy Lonicerae japonicae Flos through ITS2 sequencing

2021 ◽  
Vol 233 ◽  
pp. 02031
Author(s):  
Zhenying Liu ◽  
Yan Zhao ◽  
Lingna Wang ◽  
Gaobin Pu ◽  
Yongqing Zhang
Keyword(s):  
Internal Transcribed Spacer ◽  
Theoretical Basis ◽  
Fungal Communities ◽  
Mold Growth ◽  
Fungal Contamination ◽  
Fungal Community Structure ◽  
Class Level ◽  
First Time ◽  
Comprehensive Characterization

Lonicerae japonicae Flos is a traditional Chinese medicine that has the effect is used for clearing away heat and detoxification. When stored improperly, this medicine is susceptible to mold growth, causing fungal contamination, reducing its safety and clinical efficacy. In order to understand the fungal contamination of Lonicerae japonicae Flos, we utilized the Illumina NovaSeq6000 platform to characterize fungal communities associated with five moldy Lonicerae japonicae Flos samples from warehouses in China via the sequencing of fungal internal transcribed spacer 2 regions. These analyses led us to identify 3 phyla, 5 classes, 7 orders, 9 families, 13 genera, and 20 species of fungi in these samples. Of these, Ascomycota was the dominant phylum, while Cysticomycetes, Ascomycetes, and Staphylococci were relatively dominant at the class level, and the most prevalent genera were Aspergillus, Penicillium, Xanthomonas, Microcystis, Talaromyces, and Erysiphe with relative abundance values of 79%, 21%, 94%, 100%, and 98%. In summary, in the present study we conducted the ITS-based comprehensive characterization of fungal communities associated with Lonicerae japonicae Flos for the first time. This approach aims to facilitate the early detection of fungal contamination in Lonicerae japonicae Flos, providing a theoretical basis for the study of anti-mold technologies.

Characterization of microbial community in cold-chain hairtail fish by high-throughput sequencing technology

2021 ◽  
Author(s):  
Jiali Xing ◽  
Xiaorong Xu ◽  
Xiaohu Luo ◽  
Ruihang Zheng ◽  
Lingyan Mao ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Flora ◽  
Diversity Indices ◽  
Cold Chain ◽  
Class Level ◽  
The Family ◽  
Dominant Bacteria

Abstract: High-throughput sequencing was used to analyze the microbial communities in the muscle samples of hairtail fish to study their diversity and dynamic changes during cold-chain circulation. The results showed that the richness and diversity of the microbial community in hairtail fish had a transient decline in 0–24 h and decreased after the first rise during 24–216 h. The diversity and richness of bacteria in cold-chain hairtail fish reached the maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. In addition, the dominant bacteria were Proteobacteria in the phylum level, Gammaproteobacteria in the class level, Pseudomonadales in the order level, Pseudomonadaceae in the family level, and Pseudomonas in the genus level, and their relative abundance were 80.52%, 72.11%, 76.68%, 23.25%, and 53.50%, respectively. In this study, the structure of bacterial flora and the dominant bacteria in cold-chain hairtail fish were analyzed by high-throughput sequencing to provide a basis for exploring how to maintain the freshness of hairtail fish and for predicting the shelf-life of hairtail fish.

Microbiota community assembly in wild rice (Oryza longistaminata) in response to drought stress shows phylogenetic conservation, stochasticity, and aboveground-belowground patterns

2020 ◽  
Author(s):  
xia ding ◽  
Xiaojue Peng ◽  
Zhichao Chen ◽  
Yingjie Li ◽  
Lihui Mao ◽  
...  
Keyword(s):  
Microbial Community ◽  
Drought Stress ◽  
Microbial Communities ◽  
Community Assembly ◽  
Wild Rice ◽  
Microbial Community Composition ◽  
Α Diversity ◽  
Oryza Longistaminata ◽  
Network Properties ◽  
Microbiome Assembly

Abstract Background Drought is a global environmental stress that limits crop yields. Microbial communities control many biogeochemical processes, and a predictive understanding of how crop microbial communities assemble in response to drought stress is central to addressing the challenges caused by drought. Little is known about the microbiome assembly processes in rice-ecosystems, particularly with regard to their environmental adaptation. Wild rice may serve as a source of superior drought tolerance candidate for rice breeding. There is an urgent need to explore wild rice resistance mechanisms to drought stress. Here, we evaluated the effect of drought stress on the microbial community recruitment and assembly in the endosphere (leaf, stem, and root) and rhizosphere of Oryza longistaminata. Results Species replacement was the dominant process shaping microbial community composition under drought stress. O. longistaminata recruited the phyla Actinobacteria and Fusobacteria, the genus Streptomyces, and phototrophic prokaryotes to improve its fitness. The host exerted strong effects on microbiome assembly, and the responses of the microbial community structure to the drought environment showed above- and belowground patterns. Drought reduced taxonomic α-diversity and destabilized co-occurrence network properties in the leaves and stems, but not in the roots and rhizosphere. Drought promoted the restructuring and strengthening of belowground network links to more strongly interconnect network properties. The drought response of the microbiome was phylogenetically conserved. Stochastic (neutral) processes acted on microbial community reassembly in response to drought stress across all four compartments. Conclusions Our results provide new insight into the mechanisms through which drought alters microbial community assembly in drought-tolerant wild rice and reveal a potential strategy for manipulating plant microbiomes to improve crop fitness.

scholarly journals Effects of Typhoon Paths on Storm Surge and Coastal Inundation in the Pearl River Estuary, China

2020 ◽  
Vol 12 (11) ◽  
pp. 1851
Author(s):  
Mei Du ◽  
Yijun Hou ◽  
Po Hu ◽  
Kai Wang
Keyword(s):  
Storm Surge ◽  
River Estuary ◽  
Pearl River Estuary ◽  
Pearl River ◽  
Coastal Inundation ◽  
The Pearl River Estuary ◽  
Typhoon Center ◽  
The Pearl River ◽  
Holland Model ◽  
First Time

A coastal inundation simulation system was developed for the coast of the Pearl River estuary (PRE), which consists of an assimilation typhoon model and the coupled ADCIRC (Advanced Circulation) + SWAN (Simulating Waves Nearshore) model. The assimilation typhoon model consists of the Holland model and the analysis products of satellite images. This is the first time an assimilation typhoon model has been implemented and tested for coastal inundation via case studies. The simulation results of the system agree well with the real measurements. Three observed typhoon paths (Hope, Nida, and Hato) were chosen to be the studied paths based on their positions relative to the PRE, China. By comparing the results of experiments with different forcing fields, we determined that the storm surge and the coastal inundation were mainly induced by wind forcing. By simulating coastal inundation for different typhoon center speeds, the Hato3 path most easily causes coastal inundation in the PRE. Moreover, the moving speed of the typhoon’s center significantly affects the coastal inundation in the PRE. The inundation becomes very serious as the movement of the typhoon center was slow down. This study provides a new reference for future predictions of coastal inundations.

scholarly journals Hydrogeological setting of a Rome city sector: shallow groundwater in the right side of Tiber River inside the G.R.A highway

2019 ◽  
Author(s):  
Adele Clausi ◽  
Roberto Mazza ◽  
Francesco La Vigna ◽  
Isidoro Bonfà
Keyword(s):  
Groundwater Resources ◽  
Shallow Groundwater ◽  
Hydrogeological Model ◽  
Detailed Model ◽  
Groundwater Flow Systems ◽  
Hydrogeological Study ◽  
Tiber River ◽  
Hydrogeological Map ◽  
The Right ◽  
First Time

This paper presents a detailed hydrogeological study of a Rome city sector, in the right side of Tiber River, inside the G.R.A. highway. A hydrogeological model of this city sector has been developed through geologic-stratigraphical analysis also of data provided by the Environmental Protection Department of Roma Capitale (Municipality of Rome) and the observation of historical topographic and thematic maps. This model is more detailed than the most recent Hydrogeological Map of Rome (1:50.000 scale), published in 2015, and allowed identifying the shallowest groundwater flow systems for the first time. This detailed model can be a very useful tool for agencies and administrations managing the protection of groundwater resources.

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