scholarly journals Investigation and analysis of the fungal community structure in moldy Lonicerae japonicae Flos through ITS2 sequencing

2021 ◽  
Vol 233 ◽  
pp. 02031
Author(s):  
Zhenying Liu ◽  
Yan Zhao ◽  
Lingna Wang ◽  
Gaobin Pu ◽  
Yongqing Zhang
Keyword(s):  
Internal Transcribed Spacer ◽  
Theoretical Basis ◽  
Fungal Communities ◽  
Mold Growth ◽  
Fungal Contamination ◽  
Fungal Community Structure ◽  
Class Level ◽  
First Time ◽  
Comprehensive Characterization

Lonicerae japonicae Flos is a traditional Chinese medicine that has the effect is used for clearing away heat and detoxification. When stored improperly, this medicine is susceptible to mold growth, causing fungal contamination, reducing its safety and clinical efficacy. In order to understand the fungal contamination of Lonicerae japonicae Flos, we utilized the Illumina NovaSeq6000 platform to characterize fungal communities associated with five moldy Lonicerae japonicae Flos samples from warehouses in China via the sequencing of fungal internal transcribed spacer 2 regions. These analyses led us to identify 3 phyla, 5 classes, 7 orders, 9 families, 13 genera, and 20 species of fungi in these samples. Of these, Ascomycota was the dominant phylum, while Cysticomycetes, Ascomycetes, and Staphylococci were relatively dominant at the class level, and the most prevalent genera were Aspergillus, Penicillium, Xanthomonas, Microcystis, Talaromyces, and Erysiphe with relative abundance values of 79%, 21%, 94%, 100%, and 98%. In summary, in the present study we conducted the ITS-based comprehensive characterization of fungal communities associated with Lonicerae japonicae Flos for the first time. This approach aims to facilitate the early detection of fungal contamination in Lonicerae japonicae Flos, providing a theoretical basis for the study of anti-mold technologies.

Morphological variability and molecular characterization of Mawsonascaris australis (Johnston & Mawson, 1943) (Nematoda: Ascaridoidea: Acanthocheilidae) from the brown guitarfish Rhinobatos schlegelii Müller & Henle (Rhinopristiformes: Rhinobatidae)

2017 ◽  
Vol 92 (6) ◽  
pp. 760-764 ◽  
Author(s):  
W.-T. Zhao ◽  
Z. Xu ◽  
L. Li
Keyword(s):  
Internal Transcribed Spacer ◽  
Morphological Variability ◽  
Intraspecific Variability ◽  
Interspecific Variation ◽  
Fish Host ◽  
Molecular Approaches ◽  
The Taiwan Strait ◽  
The Right ◽  
First Time

AbstractA rare ascaridoid species, Mawsonascaris australis (Johnston & Mawson, 1943), is reported from the Taiwan Strait for the first time, and the brown guitarfish Rhinobatos schlegelii Müller & Henle (Rhinopristiformes: Rhinobatidae) represents a new fish host. The detailed morphology of M. australis was studied using light and scanning electron microscopy (SEM) based on the newly collected specimens. The results revealed some important, but previously unreported or erroneous, morphological features, including the lateral alae starting well posterior to the base of the ventrolateral lips, the presence of one pair of double paracloacal papillae and a single, small precloacal median papilla on the anterior margin of the cloaca. In addition, in order to determine whether the morphometric difference in the relative lengths of the right and left spicules represents intraspecific or interspecific variation, specimens of M. australis were characterized using molecular approaches by sequencing and analysing the ribosomal internal transcribed spacer (ITS) and mitochondrial cox1 genes. The results indicate that this morphometric difference represents intraspecific variability.

scholarly journals Biodiversity of Dickeya spp. Isolated from Potato Plants and Water Sources in Temperate Climate

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 408-417 ◽  
Author(s):  
Marta Potrykus ◽  
Malgorzata Golanowska ◽  
Wojciech Sledz ◽  
Sabina Zoledowska ◽  
Agata Motyka ◽  
...  
Keyword(s):  
Surface Waters ◽  
Soft Rot ◽  
Water Sources ◽  
Temperate Climate ◽  
Potato Processing ◽  
Potato Plants ◽  
Dickeya Solani ◽  
First Time ◽  
Comprehensive Characterization

Bacteria from the genera Dickeya (formerly Erwinia chrysanthemi) and Pectobacterium (formerly E. carotovora) are the agents of blackleg and soft rot on many important crops. In 2005, Dickeya solani was isolated for the first time in Poland from a symptomatic potato plant. To establish the presence and diversity of Dickeya spp. in Poland, we surveyed potato fields and water sources, including surface waters near potato fields and water from potato-processing facilities and sewage plants. Only D. dianthicola and D. solani were isolated from symptomatic potato, and only D. zeae and D. chrysanthemi were isolated from water sources. The Dickeya spp. isolated from potato formed a relatively homogenous group, while those from water sources were more diverse. To our knowledge, this is the first comprehensive characterization of Dickeya spp. isolated during several years from regions with a temperate climate in Central Europe.

scholarly journals Genetic and Epigenetic Factors of Takotsubo Syndrome: A Systematic Review

2021 ◽  
Vol 22 (18) ◽  
pp. 9875
Author(s):  
Valentina Ferradini ◽  
Davide Vacca ◽  
Beatrice Belmonte ◽  
Ruggiero Mango ◽  
Letizia Scola ◽  
...  
Keyword(s):  
Systematic Review ◽  
Genetic Basis ◽  
Left Ventricular ◽  
Takotsubo Syndrome ◽  
Epigenetic Factors ◽  
Complete Knowledge ◽  
Apical Balloon Syndrome ◽  
First Time ◽  
Comprehensive Characterization

Takotsubo syndrome (TTS), recognized as stress’s cardiomyopathy, or as left ventricular apical balloon syndrome in recent years, is a rare pathology, described for the first time by Japanese researchers in 1990. TTS is characterized by an interindividual heterogeneity in onset and progression, and by strong predominance in postmenopausal women. The clear causes of these TTS features are uncertain, given the limited understanding of this intriguing syndrome until now. However, the increasing frequency of TTS cases in recent years, and particularly correlated to the SARS-CoV-2 pandemic, leads us to the imperative necessity both of a complete knowledge of TTS pathophysiology for identifying biomarkers facilitating its management, and of targets for specific and effective treatments. The suspect of a genetic basis in TTS pathogenesis has been evidenced. Accordingly, familial forms of TTS have been described. However, a systematic and comprehensive characterization of the genetic or epigenetic factors significantly associated with TTS is lacking. Thus, we here conducted a systematic review of the literature before June 2021, to contribute to the identification of potential genetic and epigenetic factors associated with TTS. Interesting data were evidenced, but few in number and with diverse limitations. Consequently, we concluded that further work is needed to address the gaps discussed, and clear evidence may arrive by using multi-omics investigations.

Pigments of Fly Agaric (Amanita muscaria)

2007 ◽  
Vol 62 (11-12) ◽  
pp. 779-785 ◽  
Author(s):  
Florian Stintzing ◽  
Willibald Schliemann
Keyword(s):  
Complete Characterization ◽  
Pigment Composition ◽  
High Susceptibility ◽  
Amanita Muscaria ◽  
Pigment Pattern ◽  
Starting Point ◽  
First Time ◽  
Derivatives Of ◽  
Comprehensive Characterization

The complex pigment pattern of fly agaric (Amanita muscaria) cap skins has been studied by LC-DAD and mass spectrometry. Among the betaxanthins the corresponding derivatives of serine, threonine, ethanolamine, alanine, Dopa, phenylalanine and tryptophan are reported for the first time to contribute to the pigment pattern of fly agarics. Betalamic acid, the chromophoric precursor of betaxanthins and betacyanins, muscaflavin and seco-dopas were also detected. Furthermore, the red-purple muscapurpurin and the red muscarubrin were tentatively assigned while further six betacyanin-like components could not be structurally allocated. Stability studies indicated a high susceptibility of pigment extracts to degradation which led to rapid colour loss thus rendering a complete characterization of betacyaninlike compounds impossible at present. Taking into account these difficulties the presented results may be a starting point for a comprehensive characterization of the pigment composition of fly agarics.

scholarly journals The Use of Selective Laser Melting to Increase the Performance of AlSi9Cu3Fe Alloy

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1918 ◽  
Author(s):  
Michaela Fousova ◽  
Drahomir Dvorsky ◽  
Marek Vronka ◽  
Dalibor Vojtech ◽  
Pavel Lejcek
Keyword(s):  
Electron Microscopy ◽  
Selective Laser Melting ◽  
Analytical Techniques ◽  
Static Tension ◽  
Laser Melting ◽  
Transmission Electron ◽  
Pressure Die Casting ◽  
First Time ◽  
Comprehensive Characterization

For the first time, the comprehensive characterization of the additively manufactured AlSi9Cu3Fe alloy is reported in this paper. Conventionally, the AlSi9Cu3(Fe) alloy is prepared by high-pressure die casting (HPDC), but this technology largely does not offer such opportunities as additive manufacturing (AM) does, especially in the design of new lightweight parts. In the present paper, testing samples were prepared by selective laser melting (SLM), one of the AM technologies, and characterized in terms of their microstructure (by means of light microscopy, scanning electron microscopy and transmission electron microscopy in combination with analytical techniques for evaluation of chemical and phase composition) and mechanical properties (static tension, compression, and hardness). All the characteristics were compared with the HPDC reference material. Our study showed an excellent improvement both in strength (374 ± 11 MPa compared to 257 ± 17 MPa) and plasticity (1.9 ± 0.2% compared to 1.2 ± 0.5%) of the material thanks to its very fine and distinctive microstructure.

Characterization of rhizosphere and endophytic fungal communities from roots ofStipa purpureain alpine steppe around Qinghai Lake

2016 ◽  
Vol 62 (8) ◽  
pp. 643-656 ◽  
Author(s):  
Dengxue Lu ◽  
Hui Jin ◽  
Xiaoyan Yang ◽  
Denghong Zhang ◽  
Zhiqiang Yan ◽  
...  
Keyword(s):  
Rhizosphere Soil ◽  
Fungal Communities ◽  
Qinghai Lake ◽  
Fungal Community Structure ◽  
Operational Taxonomic Units ◽  
Alpine Steppe ◽  
Stipa Purpurea ◽  
Xizang Plateau ◽  
Fungal Assemblages

Stipa purpurea is among constructive endemic species in the alpine steppe on the Qinghai–Xizang Plateau. To reveal the fungal community structure and diversity in the rhizosphere and roots of this important grass and to analyze the potential influence of different habitats on the structure of fungal communities, we explored the root endophyte and the directly associated rhizosphere communities of S. purpurea by using internal transcribed spacer rRNA cloning and sequencing methods. We found that the roots of S. purpurea are associated with a diverse consortium of Basidiomycota (59.8%) and Ascomycota (38.5%). Most fungi obtained from rhizosphere soil in S. purpurea have been identified as Ascomycetes, while the high proportion detected in roots were basidiomycetous endophytes. The species richness, diversity, and evenness of fungal assemblages were higher in roots than in the rhizosphere soil. Fungi inhabiting the rhizosphere and roots of S. purpurea are significantly different, and the rhizosphere and endophyte communities are largely independent with little overlap in the dominant phyla or operational taxonomic units. Taken together, these results suggested that a wide variety of fungal communities are associated with the roots and rhizosphere soil of S. purpurea and that the fungal assemblages are strongly influenced by different habitats.

scholarly journals Expected Distribution of Surfing Days in the Iberian Peninsula

2020 ◽  
Vol 8 (8) ◽  
pp. 599
Author(s):  
Anna Boqué Ciurana ◽  
Enric Aguilar
Keyword(s):  
Iberian Peninsula ◽  
Small Subset ◽  
Economic Activities ◽  
Science Data ◽  
Climate Services ◽  
Western Shore ◽  
Northern Shore ◽  
First Time ◽  
Comprehensive Characterization

This study presents, for the first time, a comprehensive characterization of the surf spots around the Iberian Peninsula and provides surfers and stakeholders an evaluation of the expected surfing days per year on each region and spot. The provision of this climate information can help to decision-making and limit the economic and social damages caused by climate-related disasters. This product aligns with the concept of climate services, increasingly requested to help economic activities to achieve optimal performances. We employ use in our study of two sources of data: meteorological buoys (Redcos, Redex and Costeira) and citizen science data, specifically information mined from surfers reanalyzed, namely the information contained in the Glassy app for smartphones (GAC & GAS). The surf spots are characterized using bottom type, surf break type and optimal wind (Owd) and optimal swell direction (Osd). Then, we define a surfing day as the ones in which optimal swell direction and waves bigger than 0.9 m occur; using three parameters mean swell direction (Dmd), significant wave height (Hm0) and optimal swell direction for each surf spot (Osd) and compute the expected frequency of surfing days per year. Once this is done, we attempt to validate the approach taken to characterize a surfing day using buoys parameters (Hm0, Hmax, Tp and Dmd) and information about actual surf sessions for a small subset of our spots (i.e., Costa Tarragona). Our findings confirm that the area of western shore is the best suited for surfing, with over 300 days/year, followed by northern shore (300, 200 days/year) and southern and southeastern shores (<100 days/year). We expect that these values may modestly contribute to a climate-informed planning and management of the surfing activities.

Characterization of Dicrocoelium dendriticum haplotypes from sheep and cattle in Iran based on the internal transcribed spacer 2 (ITS-2) and NADH dehydrogenase gene (nad1)

2013 ◽  
Vol 89 (2) ◽  
pp. 158-164 ◽  
Author(s):  
S. Gorjipoor ◽  
M. Moazeni ◽  
H. Sharifiyazdi
Keyword(s):  
Internal Transcribed Spacer ◽  
Nadh Dehydrogenase ◽  
Sequence Data ◽  
Geographic Location ◽  
Nucleotide Substitutions ◽  
Internal Transcribed Spacer 2 ◽  
Dicrocoelium Dendriticum ◽  
Dehydrogenase Gene ◽  
First Time

AbstractThe present study assessed whether the genetic variation among different hosts (sheep and cattle) and geographical isolates (n= 28) of Dicrocoelium dendriticum from Iran is present based on mitochondrial (nad1) and ribosomal (ITS-2) DNA markers. Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydrogenase gene (nad1) and internal transcribed spacer 2 (ITS-2), respectively. The nad1 and ITS-2 sequence data were deposited in GenBank under accession numbers, JX050110–134 and JQ966972–3. According to the results of our study, ND-D and ITS-A are established as being the predominant haplotypes of D. dendriticum in Iran. The Iranian isolates showed a higher intraspecific genetic diversity of 0–0.97% for nad1, compared to 0–0.42% for ITS-2. The alignment and comparison of nad1 and ITS-2 sequences revealed eight and one polymorphic sites, respectively. In the nad1 sequences, six were silent and two nucleotide substitutions were responsible for amino acid alterations. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are not likely useful markers for D. dendriticum haplotype classification. Consequently, sequencing results obtained from the nad1 gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of D. dendriticum worldwide.

scholarly journals The Analysis of Phenolic Compounds in Walnut Husk and Pellicle by UPLC-Q-Orbitrap HRMS and HPLC

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3013
Author(s):  
Fang Sheng ◽  
Bangyan Hu ◽  
Qiang Jin ◽  
Jiangbo Wang ◽  
Cuiyun Wu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Development Stage ◽  
Walnut Husk ◽  
First Time ◽  
Comprehensive Characterization ◽  
Product Industry

Husk and pellicle as the agri-food waste in the walnut-product industry are in soaring demand because of their rich polyphenol content. This study investigated the differential compounds related to walnut polyphenol between husk and pellicle during fruit development stage. By using ultra-high performance liquid chromatography-quadrupole-orbitrap (UHPLC-Q-Orbitrap), a total of 110 bioactive components, including hydrolysable tannins, flavonoids, phenolic acids and quinones, were tentatively identified, 33 of which were different between husk and pellicle. The trend of dynamic content of 16 polyphenols was clarified during walnut development stage by high-performance liquid chromatography (HPLC). This is the first time to comprehensive identification of phenolic compounds in walnut husk and pellicle, and our results indicated that the pellicle is a rich resource of polyphenols. The dynamic trend of some polyphenols was consistent with total phenols. The comprehensive characterization of walnut polyphenol and quantification of main phenolic compounds will be beneficial for understanding the potential application value of walnut and for exploiting its metabolism pathway.

scholarly journals Genetic and Epigenetic Factors of Takotsubo Syndrome: a Systematic Review

2021 ◽  
Author(s):  
Valentina Ferradini ◽  
Davide Vacca ◽  
Beatrice Belmonte ◽  
Ruggiero Mango ◽  
Giuseppe Novelli ◽  
...  
Keyword(s):  
Systematic Review ◽  
Left Ventricular ◽  
Takotsubo Syndrome ◽  
Epigenetic Factors ◽  
Familial Form ◽  
Apical Balloon Syndrome ◽  
As Stress ◽  
First Time ◽  
Comprehensive Characterization

Takotsubo syndrome (TTS), recognized as stress&rsquo;s cardiomyopathy, or better as left ventricular apical balloon syndrome in the recent years, is a rare pathology, described for the first time by Japanese researchers in 1990. TTS is characterized by an interindividual heterogeneity in onset and progression, and by the strong predominance in postmenopausal women. The clear causes of these TTS features are uncertain, given the reduced understanding of this intriguing syndrome until now. However, the increasing frequency of TTS cases in the last years, and particularly correlated to SARS-CoV-2 pandemic, leads us to imperative necessity both of a complete knowledge of TTS pathophysiology for identifying biomarkers facilitating its management, and targets for specific and effective treatments. The suspect of a genetic basis in TTS pathogenesis has been evidenced. Accordingly, familial form of TTS has been described. But a systematic and comprehensive characterization of the genetic or epigenetic factors significantly associated with TTS is lacking. Thus, we, here, conducted a systematic review of literature before June 2021, to contribute to identify potential genetic and epigenetic factors associated with TTS. Interesting data were evidenced, but of reduced number and with diverse limitations. Consequently, we concluded further work is needed to address the gaps discussed, and probably a clear evidence may arrive using multi-omics investigations.

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