scholarly journals Headspace volatiles isolated from twigs of Picea omorika from Serbia

2020 ◽  
Vol 72 (3) ◽  
pp. 445-452
Author(s):  
Biljana Nikolic ◽  
Jovana Ljujic ◽  
Srdjan Bojovic ◽  
Zorica Mitic ◽  
Nemanja Rajcevic ◽  
...  
Keyword(s):  
Natural Populations ◽  
Headspace Volatiles ◽  
Specialized Metabolites ◽  
Degree Of Separation ◽  
Germacrene D ◽  
Picea Omorika ◽  
High Degree ◽  
First Time ◽  
Two Populations ◽  
Degree Of Similarity

The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.

scholarly journals Revision of the banding sequence pool and new data on chromosomal polymorphism in natural populations of Chironomus agilis Shobanov et Djomin, 1988 (Diptera, Chironomidae)

2021 ◽  
Vol 15 (4) ◽  
pp. 527-541
Author(s):  
Veronika V. Golygina ◽  
Oksana V. Ermolaeva
Keyword(s):  
Qualitative Analysis ◽  
Natural Populations ◽  
Chromosomal Polymorphism ◽  
B Chromosomes ◽  
Medium Level ◽  
Banding Sequence ◽  
First Time ◽  
Two Populations

Quantitative and qualitative analysis of chromosomal polymorphism in 19 natural populations of Ch. agilis had been performed. Most studied populations showed a medium level of chromosomal polymorphism: on average 45±3.0% of specimens are heterozygotes with 0.52±0.01 heterozygotic inversion per larvae. Besides inversions, B-chromosomes were found in two populations. The total number of banding sequences found in banding sequence pool of Ch. agilis is 16. Three banding sequences – p’agiB3, p’agiD3, p’agiF3 – are described for the first time.

Chemical Composition and Antioxidant Activity of the Athamanta turbith ssp. haynaldii Volatiles

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Goran M. Petrović ◽  
Jelena G. Stamenković ◽  
Violeta D. Mitić ◽  
Gordana S. Stojanović ◽  
Bojan K. Zlatković ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antioxidant Compounds ◽  
Oil Composition ◽  
Headspace Volatiles ◽  
Aerial Parts ◽  
Germacrene D ◽  
Total Oil ◽  
Two Populations

The fresh aerial parts of essential oils and headspace volatiles of two populations of Athamanta turbith ssp. haynaldii were analyzed in detail by GC and GC/MS analyses. In headspace samples, 32 compounds were identified, representing 98.4% and 98.8% of the total volatiles and were dominated by hydrocarbon sesquiterpenes, representing over 60% of both. Germacrene D was the major component, followed by terpinolene and myristicin. The essential oils of A. turbith consisted mainly of phenylpropene compounds with myristicin being the major compound. Fifty two compounds were identified in the oil isolated from Perućac and 57 from Kremna, accounting for 99.0% and 99.6% of total detectable components, with myristicin (52.3% and 50.8%, respectively) being the major component. In both samples, myristicin, germacrene D and δ-cadinene make up over 78% of the total oil composition. Antioxidant activity was measured using four different assays: DPPH, ABTS, CUPRAC and TRP method. It was found that the essential oil showed low antioxidant capacities compared with standard antioxidant compounds.

scholarly journals Analysis of forest vegetation in Koviljski Rit: Comparison of habitats with varying degrees of anthropogenic influence

2016 ◽  
pp. 133-143
Author(s):  
Dusan Igic ◽  
Mirjana Cuк ◽  
Dragica Vilotic ◽  
Mirjana Sijacic ◽  
Dragica Stankovic ◽  
...  
Keyword(s):  
Natural Populations ◽  
Detrended Correspondence Analysis ◽  
Flood Plain ◽  
Forest Vegetation ◽  
Danube River ◽  
Left Bank ◽  
White Willow ◽  
The Republic ◽  
High Degree ◽  
Degree Of Similarity

Koviljski Rit is located along the left bank of the Danube River, between the villages of Kovilj and Gardinovci. It is a rare example of pristine nature, where the original features of a swamp biotope are preserved. Because of its exceptional natural value, Koviljski Rit, along with the swamp in Petrovaradin, has been under the protection of the Republic of Serbia since 1998, when it was proclaimed a Special Nature Reserve of the first category. Natural vegetation found in Koviljski Rit includes woodlands composed of willow and poplar. The majority of the reserve consists of forest plantations (55.6%), which include habitats influenced by human activities. Based on DCA (Detrended correspondence analysis) of forest vegetation in Koviljski Rit, there is a high degree of similarity between natural populations of white willow and stands of anthropogenic origin. However, in contrast, the same analysis indicates clear differences between natural and artificial poplar stands. Moreover, depending on whether they were planted in temporary or permanent flood plain habitats, significant differences are evident in the floristic structures of Euro-American poplar forests. Ecological conditions in habitats of white and Euro-American poplar (Populus x euroamericana/Dode/ Guinier) communities were found to be more similar with respect to illumination and soil humidity. However, more importantly, the anthropogenic forests recorded the lowest diversity as a result of regular thinning and measures implemented through forest management.

Ampelography - An old technique with future uses: the case of minor varieties of Vitis vinifera L. from the Balearic Islands

OENO One ◽  
2011 ◽  
Vol 45 (3) ◽  
pp. 125 ◽  
Author(s):  
Sonia Garcia-Muñoz ◽  
Gregorio Muñoz-Organero ◽  
María Teresa De Andrés ◽  
Félix Cabello
Keyword(s):  
Vitis Vinifera ◽  
Balearic Islands ◽  
Vegetative Period ◽  
Qualitative And Quantitative ◽  
Mature Leaves ◽  
High Degree ◽  
First Time ◽  
Degree Of Similarity ◽  
Somatic Mutant

<p style="text-align: justify;"><strong>Aims</strong>: We report the genetic, phenological, agronomic and ampelographic characterization of 27 minor grapevine accessions (<em>Vitis vinifera </em>L.) from the Balearic Islands (Spain). The influence of occasional climatic phenomena (hailstorm) and the ampelographer’s experience on these characteristics was studied.</p><p style="text-align: justify;"><strong>Methods and results</strong>: In 2006 and 2007, grapevine accessions were analyzed using 57 OIV (Office International de la Vigne et du Vin) qualitative and quantitative descriptors and six microsatellite loci (SSR). Ampelography is a good preliminary technique for the identification of plant material, since the ampelographic description carried out showed a high degree of similarity between the repetitions of one single accession (higher than 90%), confirming the microsatellite results. The colour of the young leaf’s upper side (OIV-051), the juiciness of the flesh (OIV-232) and the firmness of berry flesh (OIV-235) were the most difficult characters to distinguish by ampelographers. In spite of the greater similarity found among the varieties studied, there were a few strong discriminant characters (OIV-225, OIV-084, OIV-053, and OIV-004). In addition, the ampelographic descriptions, agronomic parameters and phenology were influenced by hailstorm.</p><p style="text-align: justify;"><strong>Conclusions</strong>: The combination of morphological and molecular characterizations of 27 accessions collected in the Balearic Islands (Spain) allowed their classification into 17 different vine varieties. The genetic analysis identified Beba blanca as a possible somatic mutant derived from Beba roja. The hailstorm increased the vegetative period and mostly affected mature leaves, bunches, agronomic characteristics and must composition.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: The present work characterizes, for the first time, the ampelographic and molecular profiles of these minor varieties. It is provide agronomic information about old cultivars never published before which could be interesting for the grape growers.</p>

scholarly journals A contribution to the syntaxonomic diversity of the Tazovsky Peninsula, Arctic Russia

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Michael Yu. Telyatnikov ◽  
◽  
Olga V. Khitun ◽  
Irina V. Czernyadjeva ◽  
Ekaterina Yu. Kuzmina ◽  
...  
Keyword(s):  
Southern Tundra ◽  
High Degree ◽  
First Time ◽  
Degree Of Similarity ◽  
Arctic Russia

Syntaxonomic diversity of the vegetation in the southern tundra subzone of the Tazovsky Peninsula is represented by six associations, three subassociations and three variants. Four associations (Dicrano maji–Salicetum lanatae Khitun ass. nov. hoc loco, Sphagno girgensohnii–Betuletum nanae Khitun ass. nov. hoc loco, Empetro subholarctici– Eriophoretum vaginati Khitun ass. nov. hoc loco, Eriophoro leiocarpi–Caricetum rotundatae Khitun ass. nov. hoc loco), the three subassociations (Hierochloo alpinae– Hylocomietum splendentis empetretosum subholarctici Khitun subass. nov. hoc loco, Eriophoro leiocarpi–Caricetum rotundatae typicum Khitun subass. nov. hoc loco, Eriophoro leiocarpi–Caricetum rotundatae caricetosum chordorrhizae Khitun subass. nov. hoc loco) and the three variants are described for the first time. A high degree of similarity was found between vegetation of the Tazovsky Peninsula and vegetation of the southern part of the Gydansky Peninsula (the typical tundra subzone). They have four of six associations in common, whereas with the northern part of the typical tundra there is only one association in common.

Dwindling status of Epimedium Elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

2018 ◽  
pp. 47-52
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Conservation Status ◽  
Natural Populations ◽  
Culture Techniques ◽  
Kashmir Himalayas ◽  
Near Future ◽  
First Time ◽  
Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

scholarly journals The Volatile Phytochemistry of Seven Native American Aromatic Medicinal Plants

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1061
Author(s):  
Sims K. Lawson ◽  
Prabodh Satyal ◽  
William N. Setzer
Keyword(s):  
Native American ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Essential Oils ◽  
Palmitic Acid ◽  
Methyl Salicylate ◽  
Chromatographic Techniques ◽  
Germacrene D ◽  
First Time ◽  
Gaultheria Procumbens

As part of our evaluation of essential oils derived from Native American medicinal plants, we have obtained the essential oils of Agastache foeniculum (Pursch) Kuntze (Lamiaceae), Gaultheria procumbens L. (Ericaceae), Heliopsis helianthoides (L.) Sweet (Asteraceae), Liatris spicata (L.) Willd. (Asteraceae), Pycnanthemum incanum (L.) Michx. (Lamiaceae), Smallanthus uvedalia (L.) Mack. ex Mack. (Asteraceae), and Verbena hastata L. (Verbenaceae) by hydrodistillation. The essential oils were analyzed by gas chromatographic techniques. The essential oil of A. foeniculum was dominated by estragole (88–93%), while methyl salicylate (91%) dominated the G. procumbens essential oil. Germacrene D was the major component in H. helianthoides (42%) and L. spicata (24%). 1,8-Cineole (31%) and α-terpineol (17%) were the main compounds in P. incanum essential oil. The essential oil of S. uvedalia showed α-pinene (24%), perillene (15%), and β-caryophyllene (17%) as major components. Verbena hastata essential oil was rich in 1-octen-3-ol (up to 29%) and palmitic acid (up to 22%). Four of these essential oils, H. helianthoides, L. spicata, P. incanum, and V. hastata, are reported for the first time. Additionally, the enantiomeric distributions of several terpenoid components have been determined.

Using Cluster Analysis to Improve Marketing Experiments

1971 ◽  
Vol 8 (3) ◽  
pp. 340-347 ◽  
Author(s):  
George S. Day ◽  
Roger M. Heeler
Keyword(s):  
Cluster Analysis ◽  
Space Representation ◽  
Reduced Space ◽  
High Degree ◽  
Degree Of Similarity ◽  
Sensitive Experiment ◽  
Selection Of

When the selection of a sample of stores or cities requires a high degree of similarity among the test units in order to ensure a sensitive experiment, the sample may no longer represent the market. These conflicting requirements can be satisfied by choosing the sample from clusters displayed in a reduced space representation of the market.

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

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