Helminthoses of goats breeding at Belgrade area

In aim of control parasitic infection of goat we started to sistematic parasitological examination of it. At Belgrade area we examined flocks of goats originated from 23 vilages from city districts Mladenovac, Lazarevac, Obrenovac, Grocka, Zemun, Surcin, Palilula, Vozdovac and Zvezdara. Using standart coprological methods we examined 221 faecal samples. At same time, with necropsy we examined 67 animals. We found the following helminthes species: Dicrocoelium dendriticum (83,25%), Moniezia expansa (52,36%), Echinococcus granulosus (cyst) (83,25%), Trchostrongylus axei (79,82%), T.colubriformis (69,57%), T. capricola (62,85%), Ostertagia circumcincta (65,23%), O. ostertagi (23,33%), Nematodirus spathiger (83,25%), N. filicolis (43,31%), Hameonchus contortus (58,95%), Skrjabinema caprae (13,28%), Chabertia ovina (44,14%), Oesophagostomum venulosum (28,39%), and Dictyocaulus filaria (56,45%).

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Antioxidant, Antiprotoscolices Activity of Ethanolic Extracts of Some Medicinal Plants Against Echinococcus granulosus as Eco-friendly System

Asian Journal of Water Environment and Pollution ◽

10.3233/ajw210047 ◽

2021 ◽

Vol 18 (4) ◽

pp. 87-94

Author(s):

Amer Abed ◽

Orooba Ibrahim

Keyword(s):

Medicinal Plants ◽

Echinococcus Granulosus ◽

Parasitic Infection ◽

Public Health Problem ◽

Good Alternative ◽

Thymus Vulgaris ◽

Mentha Spicata ◽

Time Dependent Effect ◽

Promising Source

The principal objective of the study is to investigate the use of Mentha spicata, Thymus vulgaris and Ocimum basilicum as a good alternative option to Antiprotoscolices in-vitro. Hydatid disease, a zoonotic parasitic infection caused by Echinococcus granulosus, an important economic and human public health problem with a wide geographical distribution. Fertile hydatid cysts were collected from livestock and the viability of the protoscolices was confirmed. Protoscolices were subjected to four different concentrations of organic extracts (12.5, 25, 50 and 75 mg/ml) for 10, 20 and 30 min. Each extract was investigated and viability of the protoscolices was tested using 0.1% eosin staining. The highest efficacy was by T. vulgaris, M. spicata (100%), and O. basilicum (98.8%) respectively, after exposure of 20 minutes at 75 mg/ml, that lead to the significant reduction in the viability of protoscolices. The extract had time-dependent effect. Phytochemical were identified qualitatively and weighted quantitatively, that help in the identification of bioactive compounds involved in selective action on the protoscolices tegument layer. In conclusion, all the selected medicinal plants could be a promising source of potent antiprotoscolices effect. The mechanism by which plant extracts killed protoscolices and also their safety for living cells are unclear and need to be investigated further.

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Taeniid cestodes in a wolf pack living in a highly anthropic hilly agro-ecosystem

Parasite ◽

10.1051/parasite/2021008 ◽

2021 ◽

Vol 28 ◽

pp. 10

Author(s):

Fabio Macchioni ◽

Francesca Coppola ◽

Federica Furzi ◽

Simona Gabrielli ◽

Samuele Baldanti ◽

...

Keyword(s):

Echinococcus Granulosus ◽

Microscopic Examination ◽

One Health ◽

Toxocara Canis ◽

Wolf Population ◽

Cestode Species ◽

Taenia Hydatigena ◽

Predator Prey ◽

Zoonotic Infections ◽

Faecal Samples

The Italian wolf population in human-modified landscapes has increased greatly in the last few decades. Anthropisation increases the risk of transmission of many zoonotic infections and in this context, control of taeniid cestode species needs to be addressed from a One Health perspective. Predator-prey interactions are at the root of taeniid cestode transmission, and the wolf plays a key role in the maintenance and transmission of taeniids. To date, all available data on the taeniids of wolves in Italy refer to populations living in a wild habitat. Between 2018 and 2019, we investigated taeniids in a wolf pack living in a highly anthropic hilly agro-ecosystem. Thirty-eight faecal samples were collected and analysed, 4 of which were also genetically characterised for individual wolves and belonged to three different animals. Samples collected were analysed microscopically and by molecular analysis in order to identify the taeniid species. Taeniid eggs were detected in 34.2% (13/38) of samples. Within samples positive to taeniid eggs only Echinococcus granulosus s.s. and Taenia hydatigena were identified in 26.3% and 10.5% of the samples, respectively. On microscopic examination, Capillaria spp., Ancylostomatidae and Toxocara canis eggs, Crenosoma vulpis larvae, and coccidian oocysts were also found. The combination of low biodiversity of taeniid species with a high occurrence of E. granulosus s.s. recorded in this study could be the consequence of a deeper link occurring between wolves and livestock in human-modified landscapes than in wild settings.

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Rapid Growth of Hydatid Cyst: A Pediatric Case Report

Iranian Journal of Parasitology ◽

10.18502/ijpa.v16i1.5538 ◽

2021 ◽

Author(s):

Mehdi FOROUGHI ◽

Ali BAHADOR ◽

Zahra BEIZAVI

Keyword(s):

Case Report ◽

Hydatid Cyst ◽

Echinococcus Granulosus ◽

Hydatid Disease ◽

Rapid Growth ◽

Pediatric Population ◽

Parasitic Infection ◽

Abdominal Discomfort ◽

Pediatric Case ◽

Worldwide Distribution

Hydatid disease is a parasitic infection caused by Echinococcus granulosus with worldwide distribution. The most affected organs are liver and lungs, but it can be detected in any other organs as well. We reported a 5-yr-old boy from Shiraz, southern Iran in 2017 who presented with abdominal discomfort. Imaging revealed multiple liver hydatid cyst and a huge kidney hydatid cyst. This case showed the possible implication of rapid growth of multiple hydatid cyst as well as unusual organ presentation in the pediatric population.

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Phytochemical analysis, Antioxidant and Antiprotoscolices potential of ethanol extracts of selected plants species against Echinococcus granulosus: In-vitro study

Open Chemistry ◽

10.1515/chem-2019-0099 ◽

2019 ◽

Vol 17 (1) ◽

pp. 874-883 ◽

Cited By ~ 3

Author(s):

Sumbal Haleem ◽

Sadaf Niaz ◽

Naveeda Akhtar Qureshi ◽

Riaz Ullah ◽

Hafiz Majid Mahmood ◽

...

Keyword(s):

Medicinal Plants ◽

Echinococcus Granulosus ◽

Parasitic Infection ◽

In Vitro Study ◽

Living Cells ◽

Phytochemical Analysis ◽

Hydatid Cysts ◽

Ethanol Extracts ◽

Promising Source

AbstractCystic Echinococossis is a serious zoonotic parasitic infection caused by Echinococcus granulosus species complex. The current study was designed to evaluate the in-vitro antiprotoscolices effect of alcoholic extracts of three selected medicinal plants including Buxus Wallichiana, Berberis vulgaris and Euphorbia heliscopia against Echinococcus granulosus. Fertile hydatid cysts were collected from livestock and viability of the protoscolices was confirmed by 0.1% eosin red stain method. Protoscolices were subjected to three different concentrations of alcoholic extracts (10mg/ml, 30mg/ ml and 50mg/ml) for 10, 20 and 30 min. The highest efficacy was shown by B. vulgaris (97.92%) followed by B. wallichiana (65.98%) and E. heliscopia (61.22%) respectively, after exposure of 30 minutes at 50mg/ml concentration, that lead to the significant reduction in the viability of protoscolices. Alkaloids, flavonoids, tannins and saponnins were identified qualitatively and weighted quantitatively, that might help in the identification of bioactive compounds involved in selective action on the tegument layer of protoscolices. Alcoholic extracts of all the three selected medicinal plants showed toxic activities against protoscolices of Echinococcus granulosus. These findings suggest that all the selected medicinal plants could be a promising source of potent antiprotoscolices effect. However, the mechanism by which plant extracts killed protoscolices and also their safety for living cells are unclear and need to be investigated further.

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Parasitic infection in blackbuck (Antilope cervicapra Linnaeus, 1758) of Blackbuck Conservation Area, Bardiya and Shuklaphanta Wildlife Reserve, Kanchanpur, Western Nepal

Nepal Journal of Environmental Science ◽

10.3126/njes.v5i0.22710 ◽

2017 ◽

Vol 5 ◽

pp. 9-17

Author(s):

Ram Bahadur Chaudhary ◽

Mahendra Maharjan

Keyword(s):

Threatened Species ◽

Parasitic Infection ◽

Conservation Area ◽

Counting Technique ◽

Faecal Samples ◽

Baseline Information ◽

Parasitic Burden ◽

Wildlife Reserve ◽

Antilope Cervicapra ◽

Near Threatened

Blackbuck (Antilope cervicapra) is Near Threatened species which are conserved in blackbuck Conservation Area (BCA), Khairapur, Bardiya and Shuklaphanta Wildlife Reserve (SWR), Kanchanpur district. The present study was conducted to determine the parasitic infection in blackbuck of BCA and SWR. A total of 150 and 70 fresh faecal samples of blackbuck were collected from BCA and SWR respectively and examined by floatation, sedimentation and Stoll's counting technique. The overall prevalence of GI parasites was found to be 90.00%. The protozoan and helminthic parasitic prevalence revealed 55% and 89% respectively in both the study area. Blackbuck of BCA were found to be infected with 12 different species of parasites which includes Entamoeba sp. (20%) and Eimeria sp. (45.33%) among protozoa; Paramphistomum sp. (25.33%) and Fasciola sp. (17.33%) among trematodes; Moniezia sp. (14%) among cestode; Trichostrongylus sp. (75.33%), Ascaris sp. (57.33%), Haemonchus sp. (18%), Strongyloides sp. (16%), Bunostomum sp. (12.67%), Trichuris sp. (6%) and Oxyuris sp. (4.67%) among nematodes; while blackbuck of SWR were found to be infected with 10 different parasitic genera, i.e. Entamoeba sp. (8.57%) and Eimeria sp. (51.43%) among protozoa; Paramphistomum sp. (38.57%), Fasciola sp. (21.43%) and Schistosoma sp. (7.14%) among trematodes; Trichostrongylus sp. (55.71%), Ascaris sp. (38.57%), Haemonchus sp. (14.28%), Strongyloides sp. (12.86%) and Bunostomum sp. (2.86%) among nematodes. The present findings provide some baseline information on the parasitic burden in Blackbuck and help to formulate appropriate strategies to mitigate the endoparasitic problem of blackbuck in SWR and BCA.

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Strategic control of Dicrocoelium dendriticum (Digenea) egg excretion by naturally infected sheep

Veterinární Medicína ◽

10.17221/63/2009-vetmed ◽

2010 ◽

Vol 55 (No. 1) ◽

pp. 19-29 ◽

Cited By ~ 10

Author(s):

MY Manga-Gonzalez ◽

H. Quiroz-Romero ◽

C. Gonzalez-Lanza ◽

B. Minambres ◽

P. Ochoa

Keyword(s):

Body Weight ◽

Infected Sheep ◽

Cold Period ◽

Oral Suspension ◽

Strategic Control ◽

Egg Survival ◽

Dicrocoelium Dendriticum ◽

Sedimentation Technique ◽

Faecal Samples ◽

Pasture Contamination

The aim of this study was to determine the most appropriate months for applying albendazole (ABZ; oral suspension dose 20 mg/kg body weight) to sheep naturally infected with Dicrocoelium dendriticum and kept at pasture, in order to reduce parasite egg shedding to a minimum, mainly during the cold months and, as a result, decrease pasture contamination by viable eggs. Five animal groups (G), homogeneous as regards the number of eggs per gram (EPG) in faeces, were established. The treatment months were: G1, November and January; G2, November and February; G3, November and April; G4, January; and G5 (control), April. Ten samplings (S1-S10) were carried out every 35 to 45 days to collect faecal samples from the rectum of each animal in the five groups. The sedimentation technique and McMaster egg counting chambers were used to analyze the faecal samples. Due to the effect of albendazole (ABZ) treatments, the five groups behaved differently with regard to EPG reduction and the percentage of samples positive for D. dendriticum eggs. Using the Kruskal-Wallis test, statistically significant differences (P < 0.05) were observed between the EPG values obtained in G5 and the rest of the groups from November to May, but not from May onwards. The biggest reduction in egg excretion was obtained in G1, mainly in the cold period when elimination is highest and egg survival greatest, so G1 gave the best result, followed by G2, G4, G3 and finally G5 in descending order.

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Zoonotic Gastrointestinal Parasites of Baboons (Papio anubis) in the Shai Hill Reserve in Ghana

BioMed Research International ◽

10.1155/2020/1083251 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

John Asiedu Larbi ◽

Stephen Akyeampong ◽

Amina Abubakari ◽

Seth Offei Addo ◽

Dinah Okoto ◽

...

Keyword(s):

Intestinal Parasites ◽

Parasitic Infection ◽

Gastrointestinal Parasites ◽

Papio Anubis ◽

Concentration Technique ◽

Faecal Samples ◽

Parasite Infections ◽

Medical Importance ◽

High Level ◽

And Control

Several intestinal parasites of baboons are of zoonotic importance, especially where there is considerable interaction between the baboons and humans. The identification of gastrointestinal parasite infections of baboons (Papio anubis) was undertaken at the Shai Hills Reserve Resort, Ghana. A total of 51 faecal samples were collected randomly from individual baboons (51) and examined for cysts, eggs, and larvae of parasites of medical importance, using the direct saline smear and formol-ether concentration technique. The baboons were in 7 troops and were grouped into juveniles, subadults, and adults. The survey showed that 92% of the baboon samples examined were infected with at least one or more parasite(s) of medical importance. Hookworm had the highest prevalence of 38.39% followed by Ascaris lumbricoides (22.32%), whilst Diphyllobotrium latum recorded the lowest prevalence of 0.89%. Adults had the highest prevalence of A. lumbricoides (35.42%). Hookworm (47.92%) occurred most in the juveniles. This is the first report of intestinal parasitic infection in baboons within the Shai Hills Reserve. The results showed a high level of multiparasitism in the nonhuman primates, thus the need for possible interventions to maintain ecosystem health and control the infections as they may be a potential source of transmission to humans.

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10-year parasitological examination results (2003 to 2012) of faecal samples from horses, ruminants, pigs, dogs, cats, rabbits and hedgehogs

Parasitology Research ◽

10.1007/s00436-017-5646-0 ◽

2017 ◽

Vol 116 (12) ◽

pp. 3315-3330 ◽

Cited By ~ 22

Author(s):

Katharina Raue ◽

Lea Heuer ◽

Claudia Böhm ◽

Sonja Wolken ◽

Christian Epe ◽

...

Keyword(s):

Parasitological Examination ◽

Faecal Samples

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Supercapacitance/Resistance Behaviors of Helminth Eggs as Reliable Recognition and Direct Differentiation Probe

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.782380 ◽

2021 ◽

Vol 9 ◽

Author(s):

Ruhollah Shaali ◽

Mohammad Mahdi Doroodmand ◽

Mohammad Moazeni

Keyword(s):

Fasciola Hepatica ◽

High Energy ◽

Rapid Identification ◽

Large Power ◽

Taenia Multiceps ◽

Parascaris Equorum ◽

Direct Differentiation ◽

Dicrocoelium Dendriticum ◽

Helminth Eggs ◽

Moniezia Expansa

Parasitic helminths are usually known as undesired pathogens, causing various diseases in both human and animal species. In this study, we explore supercapacitance/resistance behaviors as a novel probe for rapid identification and direct differentiation of Fasciola hepatica, Parascaris equorum (with and without larvae), Dicrocoelium dendriticum, Taenia multiceps, and Moniezia expansa eggs. This claim is attributed to some characteristics, such as grave supercapacitance/area, high-energy storage/area, large power/egg, huge permittivity, and great electrical break-down potential, respectively (Fasciola hepatica: 2,158, 0.485, 2.7 × 10–3, 267, 52.6, Parascaris equorum without larvae: 2,825, 0.574, 3.0 × 10–3, 351, 68.4, Parascaris equorum with larvae: 4,519, 0.716, 2.4 × 10–3, 1.96, 97.6, Dicrocoelium dendriticum: 1,581, 0.219, 2.8 × 10–3, 1.96, 48.8, Moniezia expansa: 714, 0.149, 2.2 × 10–3, 0.88, 35.2, Taenia multiceps: 3,738, 0.619, 4.7 × 10–3, 4.63, 84.4), and durable capacitance up to at least 15,000 sequential cycles at different scan rates (between 2.0 × 10−4 and 120.0 V s−1) as well as highly differentiated resistance between 400 and 600 Ω. These traits are measured by the “Blind Patch-Clamp” method, at the giga ohm sealed condition (6.18 ± 0.12 GΩ cm−1, n = 5). Significant detection ranges are detected for each capacitance and resistance with gradient limits as large as at least 880 to 1,000 mF and 400 to 600 Ω depending on the type of helminth egg. The effect of water in the structure of helminth eggs has also been investigated with acceptable reproducibility (RSD 7%–10%, n = 5). These intrinsic characteristics would provide novel facilitators for direct helminth egg identification in comparison with several methods, such as ELISA, PCR, and microscopic methods.

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Gastrointestinal parasitic infection in Gir Cattle (Bos primigenius indicus) and Jaffrabadi Buffalo (Bubalus bubalis) from Junagadh, Gujarat, India

10.21203/rs.3.rs-35376/v1 ◽

2020 ◽

Author(s):

Jatin V. Raval ◽

Riddhi M. Sachaniya

Keyword(s):

Parasitic Infection ◽

Bubalus Bubalis ◽

Gastrointestinal Parasites ◽

Parasitic Infections ◽

Infection Rates ◽

Toxocara Vitulorum ◽

Balantidium Coli ◽

Faecal Samples ◽

Buxtonella Sulcata ◽

Fasciola Spp

Abstract Coprological analysis was performed to check the presence and previlance of gastrointestinal parasites. One hundred seventy six bovine faecal samples were studied. The study reported the presence of parasitic stages of Balantidium coli, Buxtonella sulcata, Trichostrongylus spp., Toxocara vitulorum, Fasciola spp., Strongyloid spp., and Paraamphistomum spp. 43.51 % (n = 108) faecal samples from Gir cattle (Bos primigenius indicus) were positive for Gastrointestinal parasitic infections. and 36.76 % (n = 68) faecal samples from Jaffrabadi Buffaloes (Bubalus bubalis) were positive for Gastrointestinal parasitic infections. In Cattle Balantidium coli was found highest (20.37 %) and in Buffaloes Buxtonella sulcata was found highest (8.82%). the highest incidence was recorded during august for both cattle (6.48 %) and buffalo (7.35 %). Gastrointestinalparasitic infections in claves (< 12 Months) was higher than older (> 60 Months) and adult animals (12-60 Months). Season wise gastrointestinal parasitic infections in cattle were reported maximum (23.15 %) during monsoon. Maximum prevalence in buffalo was reported during Monsoon (16.18 %). similarly, females recorded higher infection rates than malesfor both cattle and buffalo.

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