scholarly journals Application of various statistical methods to analyze genetic diversity of Austrian (Pinus nigra Arn.) and Scots pine (Pinus sylvestris L.) based on protein markers

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 477-486 ◽  
Author(s):  
Aleksandar Lucic ◽  
Vasilije Isajev ◽  
Ljubinko Rakonjac ◽  
Milan Mataruga ◽  
Vojka Babic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pinus Sylvestris ◽  
Statistical Methods ◽  
Scots Pine ◽  
Correspondence Analysis ◽  
Pinus Nigra ◽  
Genetic Distances ◽  
Protein Markers ◽  
Observation Units ◽  
Precise Understanding

This paper presents the results of studies on protein polymorphism in seeds of Scots pine (Pinus sylvestris L.) and Austrian pine (Pinus nigra Arn.) as the most important economic species of the genus Pinus in Serbia. Polymorphism of protein markers was determined in selected genotypes originating from seven populations (Scots pine) and six populations (Austrian pine). Analysis of protein markers was performed using two statistical methods, NTSYS and correspondence analysis. Both methods give the same arrangement of the analyzed populations, whereby, because of a different view of genetic distances, they can and should be combined, enabling easier and more precise understanding of mutual relationships of the observation units.

scholarly journals Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers

2014 ◽  
Vol 66 (4) ◽  
pp. 1485-1492 ◽  
Author(s):  
Aleksandar Lucic ◽  
Vladan Popovic ◽  
Marija Nevenic ◽  
Danijela Ristic ◽  
Ljubinko Rakonjac ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Ssr Markers ◽  
Scots Pine ◽  
Correspondence Analysis ◽  
Genetic Similarity ◽  
Genetic Distances ◽  
Spatial Distance ◽  
Population Genetic Differentiation ◽  
Ssr Analysis ◽  
Interpopulation Variability

This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).

scholarly journals Growth and Survival Variation among Scots Pine (Pinus sylvestrisL.) Provenances

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Süleyman Gülcü ◽  
Nebi Bilir
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Native Species ◽  
Tree Height ◽  
Pinus Nigra ◽  
Black Pine ◽  
Basal Diameter ◽  
Growth And Survival ◽  
Seed Sources ◽  
Provenance Test

Tree height, basal diameter, and survival were examined in thirteen-year-old provenance test established by 30 seed sources of Scots pine (Pinus sylvestrisL.) at two exotic sites of the species in Southern part of Turkey. Variations within provenance and among provenances and relations among the traits were estimated to compare Scots pine provenance and two other native species. Averages of tree height and basal diameter were 350 cm and 52.7 mm in Aydogmus site and 385 cm and 51.2 mm in Kemer site, respectively. There were large differences within and among provenances for the characters. Sites were similar (p>0.05) for the characters, while there were significant differences (p≤0.05) among provenances within site according to results of variance analysis (ANOVA). Scots pine provenances were higher and had more thickness than that of black pine (Pinus nigraArnold) and Taurus cedar (Cedrus libaniA. Rich.) which were natural species of the region. There were positive and significant (p<0.05) correlations between height and basal diameter in the species. Average survivals were 56% and 35% of the provenances in the sites. They were 71% and 11% in black pine and 53% in Taurus cedar for the sites respectively.

scholarly journals Development and Characterisation of Irap Markers From Expressed Retrotransposon-like sequences in Pinus sylvestris L.

2014 ◽  
Vol 67 (6) ◽  
pp. 485-492 ◽  
Author(s):  
Angelika Voronova ◽  
Dainis Ruņģis
Keyword(s):  
Genetic Diversity ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Repetitive Sequences ◽  
Gene Families ◽  
Considerable Proportion ◽  
Long Terminal Repeats ◽  
Multiple Gene ◽  
Plant Genomes

AbstractConifer genomes are large and stably diploid, in contrast to angiosperms, which are more variable both in genome size and ploidy. Conifer genomes are characterised by multiple gene families and pseudogenes, contain large inter-gene regions and a considerable proportion of repetitive sequences. All members of plant retrotransposon orders have been identified in gymnosperm genomes, however active elements have not been described. Investigation of transposable elements in Scots pine (Pinus sylvestris L.) could offer insights into transposon-mediated reorganisation under stress conditions in complex and ancient plant genomes. Nine Pinus sylvestris specific markers were developed to hypothetical long terminal repeats (LTRs) from differentially expressed retrotransposon-like fragments after heat stress and insect damage. Genetic diversity of 150 trees from a naturally regenerated pine stand was investigated using the IRAP method. The developed markers revealed high levels of genetic diversity and were able to distinguish subpopulations growing in long-term differential environmental conditions. Somaclonal variation was also investigated using these markers and polymorphic fragments were identified between ramets of Scots pine clones growing in two different plantations, possibly indicating evidence of recent transposition events. Sequencing of the polymorphic fragments identified two groups of sequences containing LTR sequences of an unknown retrotransposon with homology to the LTRs of the Copia-17-PAb-I element.

scholarly journals Genetic diversity and differentiation of Pinus sylvestris L. from the IUFRO 1982 provenance trial revealed by AFLP analysis

2015 ◽  
Vol 67 (4) ◽  
pp. 1237-1249
Author(s):  
Piotr Androsiuk ◽  
Sylwia Ciaglo-Androsiuk ◽  
Lech Urbaniak
Keyword(s):  
Genetic Diversity ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Aflp Analysis ◽  
Provenance Trial ◽  
Migration Routes ◽  
Bulked Dna ◽  
Individual Character ◽  
The Individual ◽  
Diversity Studies

DNA markers have become effective tools in genetic diversity studies of forest trees. However, molecular marker analyses are associated with laborious and costly effort. One of the possibilities to overcome these constraints is to analyze bulked samples per population, rather than individual plants. We have used bulked DNA-based AFLP analysis to investigate genetic variations in Pinus sylvestris L. (Scots pine) from the IUFRO 1982 provenance trial in K?rnik (western Poland). Four AFLP primer combinations yielded a total of 309 bands, of which 208 (67.31%) were polymorphic. Thirty-six (11.65%) unique alleles were deployed randomly among the populations. Estimated genetic diversity and differentiation was high, as expressed by He = 0.238 and I = 0.356, and by genetic distance values which ranged from 0.154 to 0.363. A geographic pattern of interpopulation differentiation was observed, pointing to the individual character of populations from northeastern Europe. In the light of available data, we discuss the influence of historical migration routes, gene flow and human activity on observed genetic diversity and differentiation of Scots pine in Europe. Our results indicate that the AFLP method applied to DNA templates extracted from bulked leaf samples provides an efficient approach to elucidate genetic diversity and relationships among Scots pine populations.

scholarly journals Genetic Structure, Differentiation and Originality of Pinus sylvestris L. Populations in the East of the East European Plain

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 999
Author(s):  
Yulia Vasilyeva ◽  
Nikita Chertov ◽  
Yulia Nechaeva ◽  
Yana Sboeva ◽  
Nina Pystogova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Spatial Genetic Structure ◽  
Natural Populations ◽  
Geographic Location ◽  
East European Plain ◽  
Gene Pools ◽  
East European

In order to carry out activities aimed at conservation and rational use of forest resources; it is necessary to study the main forest-forming plant species in detail. Scots pine (Pinus sylvestris L., Pinaceae) is mainly found in the boreal forests of Eurasia and is not so often encountered in the east of the East European Plain. The aim of the study was to study the genetic diversity, structure and differentiation of Scots pine populations in the east of the East European Plain. We studied ten populations of P. sylvestris using the Inter Simple Sequence Repeats (ISSR)-based DNA polymorphism detection method. Natural populations are demonstrated by relatively high rates of genetic diversity (He = 0.167; ne = 1.279; I = 0.253). At the same time, there is a tendency for a decrease in the genetic diversity of the studied populations of P. sylvestris from west to east. Analysis of the genetic structure shows that the studied populations are highly differentiated (GST = 0.439), the intrapopulation component accounts for about 56% of the genetic diversity. Using various algorithms for determining the spatial genetic structure, it is found that the studied populations form two groups of populations in accordance with geographic location. With the help of a genetic originality coefficient, populations with specific and typical gene pools are identified. They are recommended as sources of genetic diversity and reserves for the conservation of genetic resources of the species.

scholarly journals Effects of Application of Benzyladenine to Seedlings of Pinus sylvestris and Pinus nigra

1990 ◽  
Vol 8 (4) ◽  
pp. 212-214
Author(s):  
A.A. Boe
Keyword(s):  
Plant Growth ◽  
Gibberellic Acid ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Pinus Nigra ◽  
Growth Cycle ◽  
Seedling Height ◽  
Pine Seedlings ◽  
Second Growth ◽  
Number Of Branches

Abstract Greenhouse grown seedlings of Pinus sylvestris L. (Scots pine) and Pinus nigra Arnold. (Austrian pine) were treated with plant growth regulators in a 1% solution of dimethyl sulfoxide. Benzyladenine (BA) induced branching of seedlings of Scots pine. Both Scots pine and Austrian pine seedlings were reduced in height by BA treatments. The addition of gibberellic acid (GA) to the BA treatments had little effect on branching or seedling height. After a second growth cycle the Scots pine seedlings treated with BA were shorter and had more branches while the Austrian pine seedlings were also shorter without an increase in the number of branches.

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