scholarly journals The impact of extremely low frequency electromagnetic field (50Hz, 0.25 mT) on fitness components and wing traits of Drosophila subobscura

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 967-982 ◽  
Author(s):  
Aleksandra Patenkovic ◽  
Tatjana Savic ◽  
Bojan Kenig ◽  
Zorana Kurbalija-Novicic ◽  
Marko Andjelkovic
Keyword(s):  
Biological Effects ◽  
Low Frequency ◽  
Drosophila Subobscura ◽  
Directional Asymmetry ◽  
Control Group ◽  
Past Century ◽  
Wing Size ◽  
Fitness Components ◽  
Extremely Low Frequency ◽  
The Impact

Over the past century, man-made electromagnetic fields (EMF) have changed the natural environment, representing one of the most common and rapidly increasing environmental factors as technology advances. Numerous studies of biological effects of exposure to EMF have been performed on Drosophila during the last 30 years, showing contrasting results. In an attempt to determine whether EMF exposures might affect the development of Drosophila, the present study examines phenotypic variations through direction and range of changes in several fitness components and wing length and width, of Drosophila subobscura isofemale lines, affected by 50 Hz EMF. Embryonal and early post-embryonal stages were exposed to homogeneous sinusoidal 0.25 mT EMF of 50 Hz. The EMF exposure caused a significantly prolonged developmental time and significantly increased the wing size with the opposite direction of directional asymmetry compared to the control group. These results shows that even such a weak EMF has the potential to modulate, through developmental pathways, some of the fitness-related traits, wing size, and wing asymmetry in D. subobscura even after one generation of exposure, showing that extremely low frequency (ELF) EMFs have relevant consequences on development, adult morphology, and fitness.

scholarly journals Comparing the Impact of Long-Term Exposure to Extremely Low-Frequency Electromagnetic Fields With Diverse Values on Anxiety, Memory, Learning, And Β-Amyloid Deposition In Adult Rats

2021 ◽  
pp. 1-24
Author(s):  
Nafiseh Faraji ◽  
◽  
Iraj Salehi ◽  
Akram Alizadeh ◽  
Arash Pourgholaminejad ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Amyloid Deposition ◽  
Control Group ◽  
Adult Rats ◽  
Male Adult ◽  
Extremely Low Frequency ◽  
Β Amyloid ◽  
The Impact ◽  
The Brain

Background: Extremely low-frequency electromagnetic fields (ELF-EMFs) have gathered significant consideration for their possible pathogenicity. However, their effects on nervous system functions were not fully clarified. In this study, our aim was to assay the effect of ELF-EMFs with different intensity on memory, anxiety, antioxidant activity, beta amyloid (Aβ) deposition and microglia population in rats. Materials and Methods: Fifty male adult rats were randomly separated into 5 groups; four groups exposed to a flux density of 1, 100, 500 and 2000 microtesla (µT), 50 Hertz (Hz) frequency for 1h/day for 2 months and one group as a control group. The control group were without ELF-EMF stimulation. After 8 weeks, passive avoidance and elevated plus maze (EPM) tests was performed to assess memory formation and anxiety-like behavior, respectively. Total free thiol groups and the index of lipid peroxidation were assessed. Also, for detection of β-Amyloid deposition and stained microglia in the brain, anti-β-amyloid and anti-Iba1 antibodies were used. Results: In the ELF-EMF exposure groups, the step-through latency was significantly greater in the retention test (100,500, and 2000 µT) than in the control group (P<0.05). Also, the percentage of the entries into the open arms in ELF-EMF exposure groups (especially 2000 µT) decreased than control group (P <0.05). No β-Amyloid depositions were detected in the hippocampus of different groups. An increase in microglia numbers in the 100, 500 and 2000 µT groups was observed in compare to the control and 1 µT group. Conclusion: Exposure to ELF-EMF had an anxiogenic effect on rats and promoted memory and also induced oxidative stress. No β-Amyloid depositions were detected in the brain. Also, positive effect of ELF-EMF was observed on population of microglia population in the brain.

scholarly journals The Effect of 12 Hz Extremely Low-Frequency Electromagnetic Fields on Visual Memory of Male Macaque Monkeys

2021 ◽  
Author(s):  
Masoomeh Kazemi ◽  
◽  
Hamed Aliyari ◽  
Elaheh Tekieh ◽  
Hassan Tavacoli ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Visual Memory ◽  
Rhesus Macaques ◽  
Low Frequency ◽  
Serum Levels ◽  
Control Group ◽  
Behavioral Tests ◽  
Extremely Low Frequency ◽  
Before And After ◽  
Male Rhesus

Introduction: Today, humans leave in a world surrounded by electromagnetic fields. Numerous studies have been carried out to discover the biological, physiological, and behavioral effects of electromagnetic fields on humans and animals. Given the biological similarities between monkeys and humans, the goal of the present research was to examine Visual Memory (VM), hormonal, genomic, and anatomic changes, and changes of the amygdala function in the male rhesus macaques who exposed to extremely low-frequency magnetic fields (ELF/MF). Materials and methods: Four male Rhesus Macaques (Macaca mulatta) were used. For the behavioral tests the animals were needed to be fasting for 17 hours, and for the behavioral tests such as visual memory, cooperation of the animal was necessary. Using the radiation protocol, two of the monkeys were exposed to 12-Hz electromagnetic fields with a magnitude of 0.7 microtesla (electromagnetic radiation) four hours a day for a month. Before and after the exposure, a visual memory test was conducted using a coated device (visible reward) on a movable stand. Ten mL of blood was obtained from the femoral artery of the monkeys and half of it was used to examine cortisol serum levels using MyBioSource kits (made in the USA). The other half of blood samples were used to extract lymphocytes for assaying expressions of Glucocorticoid Receptor (GR) genes before and after radiation using the PCR method. Anatomic studies of the amygdala were carried out based on pre- and post-radiation Magnetic Resonance Imaging (MRI). Findings: Research results indicated that visual memory in male primates increased significantly at the 12Hz frequency. Hormonal analysis at the 12Hz frequency showed a decrease in cortisol serum levels. However, visual memory and serum cortisol levels did not change considerably in male primates in the control group. There was no considerable amygdala volumetric difference at the 12 Hz frequency. The expression of the GR genes decreased at 12Hz compared to the control group. Conclusion: In short, these results indicated that ELF may have a beneficial value for memory enhancement as indicated by the fact that exposure to the 12 HZ ELF can enhance visual memory. This may be due to a decrease in plasma cortisol, and/or expression of GR genes. Moreover, direct involvement of the amygdala in this regard cannot be recommended.

scholarly journals PERUBAHAN EKSPRESI HEAT SHOCK PROTEIN 70 AKIBAT PAPARAN MEDAN ELEKTROMAGNETIK EXTREMELY LOW FREQUENCY PADA MAKROFAG PERITONEUM MENCIT YANG DIINFEKSI Toxoplasma gondii

Biomedika ◽  
2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Mochammad Arief Taufiqurochman
Keyword(s):  
Heat Shock ◽  
Toxoplasma Gondii ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Low Frequency ◽  
T Test ◽  
Control Group ◽  
Hsp 70 ◽  
Extremely Low Frequency ◽  
Promotor Region

Penelitian dilakukan untuk mengetahui efek paparan medan elektromagnetik ELF sebesar 100 μT 8 jam/ hari selama 2 dan 4 minggu terhadap ekspresi HSP 70 makrofag peritoneum mencit yang diinfeksi dengan Toxoplasma gondii. Jenis penelitian ini adalah Eksperimen Biomedik menggunakan rancangan randomized separate posttest control group designdengan hewan coba mencit strain Balb/c, melalui pengamatan ekspresi HSP 70 , terdiri dari 3 kelompok kontrol dan 4 kelompok perlakuan, tiap kelompok terdiri dari 4 hewan coba. Pengamatan jaringan menggunakan metode imunohistokimia indirek, hasilnya dianalisis menggunakan uji statistik Independent t-test antar kelompok setelah dilakukan uji homogenitas dan normalitas data penelitian ( α=0.05). Hasil penelitian menunjukkan bahwa akibat paparanME ELF dengan itensitas 100 μT selama 2 minggu belum mampu melemahkan atau memutus rantai DNA gen HSP 70 promotor region tetapi menimbulkan stres seluler yang berakibat teraktifasinya HSF 1 melalui konversi menjadi trimer yang akan meregulasi secara cepat sintesis HSP 70 . Paparan medan elektromegnetik ME ELF selama 4 minggu dapat melemahkan bahkan memutus rantai DNA hsp 70 promotor region, sehingga sintesis HSP akan terhambat secara signifikan (p<0.05). Terdapat peningkatan secara signifikan ekspresi HSP 70 makrofag peritoneum mencit yang terpapar ME ELF dengan itensitas 100 μT selama 2 minggu pada kelompok yang terinfeksi toxoplasma gondii dan terjadi penurunan secara signifikan ekspresi HSP 70 pada kelompok terpapar ME ELF selama4 minggu pada kelompok yang terinfeksi Toxoplasma gondi dibandingkan dengan konrol.Kata Kunci: Medan Electromagnetik ELF, HSP 70, Makrofag, Toxoplasma gondii.

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats

2020 ◽  
Vol 36 (2) ◽  
pp. 119-131
Author(s):  
Sevgi Gunes ◽  
Belgin Buyukakilli ◽  
Selma Yaman ◽  
Cagatay Han Turkseven ◽  
Ebru Ballı ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Muscle Tissue ◽  
Chronic Exposure ◽  
Low Frequency ◽  
Diaphragm Muscle ◽  
Mechanical Activity ◽  
Control Group ◽  
Female Group ◽  
Bioelectrical Activity ◽  
Extremely Low Frequency

The aim of the present study was to systematically investigate the effects of chronic exposure to extremely low-frequency electromagnetic field (ELF-EMF) on electrophysiological, histological and biochemical properties of the diaphragm muscle in rats. Twenty-nine newly weaned (24 days old, 23–80 g) female ( n = 15) and male ( n = 14) Wistar Albino rats were used in this study. The animals were randomly divided into two groups: the control group and the electromagnetic field (EMF) group. The control group was also randomly divided into two groups: the control female group and the control male group. The EMF exposure group was also randomly divided into two groups: the ELF-EMF female group and the ELF-EMF male group. The rats in the ELF-EMF groups were exposed for 4 h daily for up to 7 months to 50 Hz frequency, 1.5 mT magnetic flux density. Under these experimental conditions, electrophysiological parameters (muscle bioelectrical activity parameters: intracellular action potential and resting membrane potential and muscle mechanical activity parameter: force–frequency relationship), biochemical parameters (Na+, K+, Cl− and Ca+2 levels in the blood serum of rats; Na+-K+ ATPase enzyme-specific activities in muscle tissue; and free radical metabolism in both muscle tissue and serum) and transmission electron microscopic morphometric parameters of the diaphragm muscle were determined. We found that chronic exposure to ELF-EMF had no significant effect on the histological structure and mechanical activity of the muscle and on the majority of muscle bioelectrical activity parameters, with the exception of some parameters of muscle bioelectrical activity. However, the changes in some bioelectrical activity parameters were relatively small and unlikely to be clinically relevant.

scholarly journals Correlation between the Lunar Phase and Tail-Lifting Behavior of Lizards (Pogona vitticeps) Exposed to an Extremely Low-Frequency Electromagnetic Field

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 208 ◽  
Author(s):  
Tsutomu Nishimura ◽  
Harue Tada ◽  
Masanori Fukushima
Keyword(s):  
Magnetic Field ◽  
Full Moon ◽  
Low Frequency ◽  
Control Group ◽  
Lunar Phase ◽  
Extremely Low Frequency ◽  
K Index ◽  
Field Sensitivity ◽  
Pogona Vitticeps ◽  
New Moon

We previously showed that the agamid lizard Pogona vitticeps responded to an extremely low-frequency electromagnetic field (ELF-EMF; frequency: 6 and 8 Hz; peak magnetic field: 2.6 µT; peak electric field: 10 V/m) with tail-lifting behavior. In addition, the tail-lifting response to ELF-EMF disappeared when the parietal eyes of the lizards were covered by small round aluminum caps. This result suggests that the parietal eye contributes to light-dependent magnetoreception. In the present study, we set up an ELF-EMF group to evaluate the long-term effect of the ELF-EMF on lizards’ behavior and examine our hypothesis that exposure to ELF-EMFs increases the magnetic field sensitivity in lizards. We therefore include the lunar phase (full moon/new moon) and K index as environmental factors related to the geomagnetic field in the analysis. The number of tail lifts per individual per day was the response variable while calendar month, daily mean temperature, daily mean humidity, daily mean atmospheric pressure, full moon, new moon, and K index were the explanatory variables. We analyzed an ELF-EMF group and a control group separately. In a multiple linear regression analysis, the independent determinants associated with the number of tail lifts were the full moon, the temperature, February, March, April, and May in the ELF-EMF group and March, April, May, and June in the control group. The P. vitticeps in the ELF-EMF group responded to the full moon whereas those in the control group did not. In addition, in the ELF-EMF group, the number of tail lifts was higher on days when the K index was higher (P = 0.07) in the first period whereas there was no such tendency in either period in the control group. There is the possibility that the exposure to ELF-EMFs may increase magnetic-field sensitivity in lizards.

DETOXICATION PROPERTIES OF COMBINED FOOD ORGANIC FIBERS OBTAINED FROM SECONDARY RAW MATERIALS OF SUGAR BEET PRODUCTION

2019 ◽  
Author(s):  
М.Ю. ТАМОВА ◽  
Е.В. БАРАШКИНА ◽  
Р.А. ЖУРАВЛЕВ ◽  
Н.Р. ТРЕТЬЯКОВА ◽  
А.Х. ШАНТЫЗ ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Citric Acid ◽  
Dietary Fiber ◽  
Low Frequency ◽  
Lead Content ◽  
Control Group ◽  
Dietary Fibers ◽  
Extremely Low Frequency ◽  
White Rats ◽  
Beet Pulp

Проведена медикобиологическая оценка детоксикационных свойств комбинированных пищевых волокон (КПВ) растительного происхождения, полученных по разработанной авторами технологии. В качестве объекта исследования были КПВ из свекловичного жома: после экстракции лимонной кислотой образец 1 и после обработки ЭМП КНЧ образец 2. Для определения детоксикационной активности КПВ сформировано 3 группы беспородных белых крыс по 20 голов в каждой, которые вместе с основным кормом получали свинец в виде свинцовой соли уксусной кислоты из расчета 25,0 мг свинца/кг массы тела. 1я и 2я опытные группы получали к корму добавку КПВ в количестве 0,6 мг/кг массы тела животного. Исследования проводили в течение 62 сут. По завершении эксперимента установлено, что у крыс 1й и 2й опытных групп, получавших КПВ из свекловичного жома, прирост живой массы тела превышал аналогичный показатель контрольной группы на 32,7 и 35,8 соответственно содержание свинца в крови снизилось относительно контрольной группы на 36,3 и 43,8 соответственно содержание свинца в печени соответственно в 1,8 и 2 раза ниже по сравнению с контрольной группой животных. Установлено, что детоксикационная активность КПВ после обработки ЭМП КНЧ выше, чем после экстракции лимонной кислотой. Biomedical assessment of detoxification properties of combined dietary fiber of plant origin, obtained by the technology developed by the authors, was carried out. Combined dietary fiber from beet pulp: after extraction with citric acid sample 1 and after treatment with an extremely low frequency electromagnetic field sample 2 were the object of study. To determine the detoxification activity of combined dietary fibers 3 groups of mongrel white rats with 20 heads in each were formed. All animals in the main feed added lead in the form of lead of salt acetic acid from calculation 25,0 mg lead/kg masses body rats. Combined dietary fibers were added to the animal feed of the 1st and 2nd experimental groups in the amount of 0,6 mg/kg of animal body weight. The studies were carried out for 62 days. At the end of the experiment, it was found that in rats of the 1st and 2nd experimental groups, where combined beet pulp fibers were added to the main food, the live weight gain exceeded the same indicator of the control group by 32,7 and 35,8, respectively the lead content in the blood decreased relative to the control group by 36,3 and 43,8, respectively the lead content in the liver, respectively, was 1,8 and 2 times lower compared to the control group of animals. It was found that the detoxification activity of combined dietary fibers after treatment with an electromagnetic field of extremely low frequency is higher than after extraction with citric acid.

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