Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes

Segmented polyurethanes based on poly(dimethylsiloxane), currently used for biomedical applications, have sub-optimal biocompatibility which reduces their efficacy. Improving the endothelial cell attachment and blood-contacting properties of PDMS-based copolymers would substantially improve their clinical applications. We have studied the surface properties and in vitro biocompatibility of two series of segmented poly(urethane-dimethylsiloxane)s (SPU-PDMS) based on hydroxypropyl- and hydroxyethoxypropyl- terminated PDMS with potential applications in blood-contacting medical devices. SPU-PDMS copolymers were characterized by contact angle measurements, surface free energy determination (calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods), and atomic force microscopy. The biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact assay, before and after pre-treatment of copolymers with multicomponent protein mixture, as well as by a competitive blood-protein adsorption assay. The obtained results suggested good blood compatibility of synthesized copolymers. All copolymers exhibited good resistance to fibrinogen adsorption and all favored albumin adsorption. Copolymers based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher surface free energy, and better microphase separation in comparison with hydroxypropyl-PDMS-based copolymers, which promoted better endothelial cell attachment and growth on the surface of these polymers as compared to hydroxypropyl-PDMS-based copolymers. The results showed that SPU-PDMS copolymers display good surface properties, depending on the type of soft PDMS segments, which can be tailored for biomedical application requirements such as biomedical devices for short- and long-term uses.

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Surface Characterization of Oxygen Plasma Treated Nano-SiO2 Sol-Gel Coating UHMWPE Filaments

Materials Science Forum ◽

10.4028/www.scientific.net/msf.658.117 ◽

2010 ◽

Vol 658 ◽

pp. 117-120 ◽

Cited By ~ 1

Author(s):

Ying Chen Zhang ◽

Feng Jun Shi ◽

Jian Xin He ◽

Hong Yan Wu ◽

Yi Ping Qiu

Keyword(s):

Free Energy ◽

Plasma Treatment ◽

Ftir Spectroscopy ◽

Surface Properties ◽

Surface Free Energy ◽

Atmospheric Pressure ◽

Surface Characterization ◽

Sol Gel ◽

Atmospheric Pressure Plasma ◽

Ultrahigh Molecular Weight Polyethylene

UHMWPE filaments have a low surface free energy and therefore often require a modification of their surface properties before any use. Atmospheric pressure plasmas treatment is a convenient and environmentally friendly way to obtain these modifications by introducing new chemical groups at the surface without affecting the bulk properties. This paper studies the influence of nano-SiO2 sol-gel coating pretreatment on atmospheric pressure plasma jet (APPJ) treatment of ultrahigh molecular weight polyethylene (UHMWPE) fibers when a mixture of 100% helium and 1% oxygen used as the treatment gas. The surface properties of the plasma-treated UHMWPE filaments are characterized using contact angle measurements and ATR-FTIR spectroscopy. The UHMWPE filaments show a remarkable increase in surface free energy after plasma treatment. ATR-FTIR spectroscopy of the plasma-treated UHMWPE filaments reveals that plasma treatment introduces oxygen-containing functionalities on the UHMWPE filaments surface leading to the increased surface free energy.

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Naturally Produced Extracellular Matrix Is an Excellent Substrate for Canine Endothelial Cell Proliferation and Resistance to Shear Stress on PTFE Vascular Grafts

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665417 ◽

1997 ◽

Vol 78 (05) ◽

pp. 1392-1398 ◽

Cited By ~ 16

Author(s):

A Schneider ◽

M Chandra ◽

G Lazarovici ◽

I Vlodavsky ◽

G Merin ◽

...

Keyword(s):

Extracellular Matrix ◽

Endothelial Cells ◽

Shear Stress ◽

Endothelial Cell ◽

Cell Attachment ◽

Thrombus Formation ◽

Endothelial Cell Proliferation ◽

Ptfe Graft ◽

Vascular Prostheses ◽

Endothelial Cell Attachment

SummaryPurpose: Successful development of a vascular prosthesis lined with endothelial cells (EC) may depend on the ability of the attached cells to resist shear forces after implantation. The present study was designed to investigate EC detachment from extracellular matrix (ECM) precoated vascular prostheses, caused by shear stress in vitro and to test the performance of these grafts in vivo. Methods: Bovine aortic endothelial cells were seeded inside untreated polytetrafluoro-ethylene (PTFE) vascular graft (10 X 0.6 cm), PTFE graft precoated with fibronectin (FN), or PTFE precoated with FN and a naturally produced ECM (106 cells/graft). Sixteen hours after seeding the medium was replaced and unattached cells counted. The strength of endothelial cell attachment was evaluated by subjecting the grafts to a physiologic shear stress of 15 dynes/cm2 for 1 h. The detached cells were collected and quantitated. PTFE or EC preseeded ECM coated grafts were implanted in the common carotid arteries of dogs. Results: While little or no differences were found in the extent of endothelial cell attachment to the various grafts (79%, 87% and 94% of the cells attached to PTFE, FN precoated PTFE, or FN+ECM precoated PTFE, respectively), the number of cells retained after a shear stress was significanly increased on ECM coated PTFE (20%, 54% and 85% on PTFE, FN coated PTFE, and FN+ECM coated PTFE, respectively, p <0.01). Implantation experiments in dogs revealed a significant increase in EC coverage and a reduced incidence of thrombus formation on ECM coated grafts that were seeded with autologous saphenous vein endothelial cells prior to implantation. Conclusion: ECM coating significantly increased the strength of endothelial cell attachment to vascular prostheses subjected to shear stress. The presence of adhesive macromolecules and potent endothelial cell growth promoting factors may render the ECM a promising substrate for vascular prostheses.

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Shape Memory Polymers Containing Higher Acrylate Content Display Increased Endothelial Cell Attachment

Polymers ◽

10.3390/polym9110572 ◽

2017 ◽

Vol 9 (11) ◽

pp. 572 ◽

Cited By ~ 4

Author(s):

Tina Govindarajan ◽

Robin Shandas

Keyword(s):

Endothelial Cell ◽

Shape Memory ◽

Cell Attachment ◽

Shape Memory Polymers ◽

Endothelial Cell Attachment

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Influence of Oxygen Plasma Modification on Surface Free Energy of PMMA Films and Cell Attachment

Macromolecular Symposia ◽

10.1002/masy.200850916 ◽

2008 ◽

Vol 269 (1) ◽

pp. 128-137 ◽

Cited By ~ 28

Author(s):

Canturk Ozcan ◽

Pınar Zorlutuna ◽

Vasıf Hasirci ◽

Nesrin Hasirci

Keyword(s):

Free Energy ◽

Surface Free Energy ◽

Oxygen Plasma ◽

Cell Attachment ◽

Plasma Modification

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Serum enhancement of human endothelial cell attachment to and spreading on collagens I and IV does not require serum fibronectin or vitronectin

Journal of Cell Science ◽

10.1242/jcs.95.2.255 ◽

1990 ◽

Vol 95 (2) ◽

pp. 255-262

Author(s):

W.D. Norris ◽

J.G. Steele ◽

G. Johnson ◽

P.A. Underwood

Keyword(s):

Endothelial Cell ◽

Culture Medium ◽

Type I Collagen ◽

Cell Attachment ◽

Cell Spreading ◽

Human Endothelial Cell ◽

Collagen Type Iv ◽

Type I ◽

Type Iv ◽

Endothelial Cell Attachment

The initial attachment and spreading of endothelial cells from human umbilical artery onto type I collagen, type IV collagen or gelatin substrata was shown to be enhanced by inclusion of serum in the culture medium. To test whether this serum effect was mediated by adsorption of serum fibronectin or vitronectin onto the collagen, these adhesive glycoproteins were selectively removed from the serum prior to addition to the culture medium. The stimulatory effect of serum on human endothelial cell spreading on collagens I and IV was also observed with serum from which either fibronectin or vitronectin, or both, had been selectively removed. The stimulatory effect for cell spreading on gelatin was diminished by selective removal of serum fibronectin, but unaffected by removal of vitronectin. Human endothelial cell attachment and spreading onto tissue culture plastic was abolished by removal of vitronectin from the serum in the culture medium. These results emphasize that the native structure of collagens is required for serum-enhancement of human endothelial cell attachment and spreading on native collagen types I and IV, and show that on these substrata the stimulated adhesion and spreading are not dependent upon adsorption of serum fibronectin or vitronectin onto the collagen substratum.

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Determination of Endothelial Cell Attachment and Proliferation on Nanofiber Based Microtubes Modified with Laminin-Derived Peptide

2019 Medical Technologies Congress (TIPTEKNO) ◽

10.1109/tiptekno.2019.8895230 ◽

2019 ◽

Author(s):

Asena Gulenay Tatar ◽

Gunnur Onak ◽

Ozan Karaman

Keyword(s):

Endothelial Cell ◽

Cell Attachment ◽

Endothelial Cell Attachment

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Bioactive nanocomposites of bacterial cellulose and natural hydrocolloids

Journal of Materials Chemistry B ◽

10.1039/c4tb00706a ◽

2014 ◽

Vol 2 (40) ◽

pp. 7034-7044 ◽

Cited By ~ 12

Author(s):

Marco Aurelio Woehl ◽

Lucy Ono ◽

Izabel Cristina Riegel Vidotti ◽

Fernando Wypych ◽

Wido Herwig Schreiner ◽

...

Keyword(s):

Free Energy ◽

Cell Growth ◽

Bacterial Cellulose ◽

Surface Properties ◽

Surface Free Energy ◽

Growth Substrates

Bacterial cellulose–natural hydrocolloid bionanocomposites were developed as cell growth substrates. The surface free energy of the composites is related to fibroblast viability. Surface properties of the bionanocomposites can be adjusted purely by changing the component proportions.

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