scholarly journals Clinical evaluation of oxidative stress in patients with diabetes mellitus type II: Impact of acute exercise

2009 ◽  
Vol 66 (6) ◽  
pp. 459-464 ◽  
Author(s):  
Nada Kostic ◽  
Zorica Caparevic ◽  
Djordje Marina ◽  
Sanja Ilic ◽  
Jana Radojkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Exercise Test ◽  
Acute Exercise ◽  
Diabetes Mellitus Type ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Patients With Diabetes ◽  
Type 2 Diabetic

Background/Aim. Exercise is a well recognized model of oxidative stress and, also, an important tool in diabetes management. The aim of our study was to evaluate oxidative stress in patients with diabetes mellitus type 2 and to determine influence of acute exercise training on the investigated parameters. Methods. To evaluate oxidative stress in the patients, we determinated following parameters: triglycerides (TG), total cholesterol, low density lipoprotein cholesterol (LDL), oxidized LDL cholesterol (Ox LDL), superoxide dismutase (SOD), glutathione peroxidase (GSHPx), plasminogen activator inhibitor (PAI) which were measured at rest and immediately after the acute bout of cardiopulmonary exercise cycle-ergometer test. Results. In basal condition, diabetic patients compared to controls have significant higher values of TG (3.12 ? 1.09 vs 1.74 ? 0.9 mmol/L, p < 0.01), Ox LDL (84.73 ? 16.90 vs 79.00 ? 29.26 mmol/L, p < 0.05) and SOD enzyme activity (913.38 ? 120.36 vs 877.14 ? 153.18 U/g Hb, p < 0.05). During the acute exercise test, there was significant increase of Ox LDL in both the study patients (from 84.73 ? 16.90 to 92.33 ? 23.29 mmol/L, p < 0.05) and in the control group (from 79.00 ? 29.26 to 89.30 ? 29.07 mmol/L, p < 0.05). SOD activity was significantly increased in both groups during exercise, in diabetic patients from 913.38 ? 120.36 to 921.50 ? 130.03 U/gHb, p < 0.05, and in the controls from 877.14 ? 153.18 to 895.00 ? 193.49, U/gHb, p < 0.05. GSH-Px activity was significantly increased only in the diabetic patients after the acute exercise (from 45.04 ? 11.19 to 51.81 ? 15.07 U/gHb, p < 0.01), but not in the controls (from 44.63 ? 13.73 to 43.97 ? 25.97 U/gHb, p = ns). PAI significantly decreased during the exercise test, only in the healthy subjects (from 2.60 ? 0.35 to 2.22 ? 0.65, p < 0.05). Type 2 diabetic patients with complications had only significant increase in GSH-Px activity (from 47.10 ? 7.37 to 54.52 ? 11.97 U/gHb, p < 0.01). Conclusion. Elevated Ox LDL, SOD and GSH-Px levels are associated with acute exercise in type 2 diabetic patients. We suggest that it could be a compensatory mechanism to preventing free radicals tissue damage. We hypothesize that a physical training program induces an enhance of muscular and liver antioxidant enzymes activity and reduces oxidative stress. Further studies are needed to explore the relationship between exercise and antioxidant system in diabetic patients with and without complications.

scholarly journals Exercise-induced oxidative stress and antioxidant enzyme activity in type 2 diabetic patients with and without diastolic dysfunction and hypertension

2009 ◽  
Vol 137 (3-4) ◽  
pp. 146-151 ◽  
Author(s):  
Nada Kostic ◽  
Zorica Caparevic ◽  
Sanja Ilic ◽  
Biljana Pencic ◽  
Jana Radojkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Exercise Test ◽  
Acute Exercise ◽  
Left Ventricular ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Dm Type 2 ◽  
Type 2 Diabetic

Introduction. Antioxidant systems are important factors affecting the oxidation of lipoproteins and thereby the progression of atherosclerotic disease. It has been suggested that physical activity might maintain and promote the antioxidant defence capacity against the oxidative stress. Left ventricular dysfunction (LVDD) and hypertension are more common in subjects with diabetes mellitus (DM) type 2. Objective. To evaluate the oxidative stress in patients with DM type 2, particularly with LVDD and hypertension and to determine the influence of acute exercise training on the investigated parameters. Methods. To assess the oxidative stress of patients, we determined the following antioxidative parameters: triglycerides (TG), total cholesterol, low density cholesterol, OxLDL cholesterol, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), plasminogen activator-type 1 (PAI-1) which were measured at rest and immediately after the acute bout of the cardiopulmonary exercise cycle ergometer test. Results. In basal conditions, diabetic patients had a significant increase of TG (3.12?1.09 vs 1.74?0.9 mmol/l; p<0.01), OxLDL cholesterol (84.73?16.9 vs 79.00?29.26 mmol/l; p<0.05) and SOD enzyme activity (913.38?120.36 vs 877.14 ?153.18; p<0.05) compared to controls. During the acute exercise test, there were significantly greater levels of OxLDL (84.73?16.90 vs 92.33?23.29 mmol/l; p<0.05) in study patients. SOD significantly increased in both groups during exercise, in diabetic patients (913.38?120.36 vs 921.50?130.03 U/g Hb; p<0.05) and in controls (877.14?153.18 vs 895.00?193.49 U/g Hb; p<0.05). GSH-Px significantly increased only in diabetic patients after acute exercise (45.04?11.19 vs 51.81?15.07 U/g Hb; p<0.01), but not in controls. PAI significantly decreased during the exercise test only in healthy subjects (2.60?0.35 vs 2.22?0.65; p<0.05). Type 2 diabetic patients with cardiovascular complications (LVDD and hypertension) had a significant increase of GSH-Px activity (47.10?7.37 vs 54.52?11.97 U/g Hb; p<0.01). Conclusion. Elevated enzyme levels are associated with exercise in type 2 diabetic patients. We suggest that it could be a compensatory mechanism to prevent free radical tissue damage. We hypothesize that a physical training programme induces the enhancement of muscular and liver antioxidant enzymes and reduces the oxidative stress.

COMPARATIVE HEMATOLOGICAL STUDY OF TYPE 2 DIABETIC AND NON-DIABETIC PATIENTS IN ERODE DISTRICT, TAMIL NADU

2021 ◽  
pp. 29-30
Author(s):  
Murugesan Thinakaran ◽  
Thangadurai Chitra
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Tamil Nadu ◽  
Haematological Parameters ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Hematological Indices ◽  
Patients With Diabetes ◽  
Type 2 Diabetic

Background And Objectives: Previous studies have shown that hematological alterations are a common nding in patients with diabetes. To aim of our study was to explore the hematological indices in type 2 diabetic patients compared with non-diabetic individual. Establish the role of haematological parameters as an early prevention strategy. Materialand Method: Two hundred and forty-six subjects were recruited for this study, one hundred and fourteen with type 2 diabetes and One hundred and thirty-two non-diabetes, were evaluated for haematological parameters using hematologic analyzer. All the information's about the disease was collected with the knowledge of the patients form the concern hospital and laboratory. Results And Conclusions: Results were compared with the same measurements in 132 subjects without diabetes mellitus. The haematological prole associated with Type2 diabetic patients signicantly reduced Hb, RBCs, PCV and MCV than Non diabetes. Increased MCHC and WBCs were noted in Type2 diabetes. Our ndings suggest the need of screening for routine hematological tests in type 2 diabetes mellitus

scholarly journals L-Cysteine Influx in Type 2 Diabetic Erythrocytes

2010 ◽  
Vol 56 (5) ◽  
pp. 545-551
Author(s):  
S.I. Rizvi ◽  
N. Srivastava
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Amino Acid ◽  
Antioxidant Defense ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Limiting Amino Acid ◽  
Type 2 Diabetic

Erythrocyte oxidative stress has been implicated in the pathogenesis of diabetes mellitus, and the deficiency of antioxidant defense by the glutathione (GSH) pathway is thought to be one of the factors responsible for development of complications in diabetes. Erythrocytes require L-cysteine for the synthesis of GSH and the rate of synthesis is determined only by L-cysteine availability. In the present study we have found that the L-cysteine influx in erythrocytes from type 2 diabetic patients was significantly lower compared to age-matched controls. The decreased influx may be one of the factors leading to low GSH concentration observed in type 2 diabetes. Since L-cysteine is the limiting amino acid in GSH synthesis, any strategy aimed to increase L-cysteine influx in erythrocytes may be beneficial for type 2 diabetic patients.

scholarly journals Total antioxidant status in type 2 diabetes mellitus with diabetic nephropathy

2019 ◽  
Vol 6 (3) ◽  
pp. 673
Author(s):  
Heena Singla ◽  
Gitanjali Goyal ◽  
Cheenu Garg ◽  
Kajal Bhalla
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Total Antioxidant ◽  
Type 2 Diabetic

Background: Diabetes mellitus has emerged as one of the most common health hazard all over the world. Diabetic nephropathy is the most challenging long term complication of Type 2 Diabetes mellitus and microalbuminuria is the earliest marker of diabetic nephropathy. In diabetes, chronic hyperglycemia and deranged lipid profile lead to excess generation of free radicals. The increased oxidative stress plays a major role in pathogenesis of diabetic complications, including diabetic nephropathy. There are many naturally occurring antioxidant enzymes in our body. Diabetes has multiple effects on protein levels and activity of these antioxidant enzymes. This further augments the oxidative stress. There are many non-enzymatic antioxidants in our body which include vitamins A, C, E and trace minerals like copper, zinc, manganese and selenium.Methods: The study was done on a total of 150 subjects. Group A comprised of 60 Type 2 diabetic patients with diabetic nephropathy, Group B comprised of 60 Type 2 diabetic patients without diabetic nephropathy and Group C comprised 30 healthy controls. Total antioxidant status, microalbuminuria and glycosylated haemoglobin were measured.Results: In present study, authors found that total antioxidant status is drastically reduced in all diabetic patients, and it was found to be further low in patients with diabetic nephropathy. This decrease was found to be directly proportional to the degree of diabetic nephropathy, as measured by the levels of microalbuminuria.Conclusions: Timely institution of antioxidant supplementation therapy may emerge as a promising measure in delaying the onset and progression of diabetic complications, especially diabetic nephropathy.

Markers of Oxidative Stress in the Saliva of Type 2 Diabetic Patients

2021 ◽  
Vol 7 (2) ◽  
pp. 1-4
Author(s):  
Olatunde Olayanju ◽  
Victory Edem ◽  
Chika Okwor ◽  
Gabriel Odok ◽  
Nnaemeka Awah
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Logistic Regression ◽  
Diabetic Patients ◽  
Oral Diseases ◽  
Operating Characteristics ◽  
Type 2 Diabetic Patients ◽  
Markers Of Oxidative Stress ◽  
Type 2 Diabetic

Objective: Diabetes mellitus is associated with increased prevalence of oral diseases for which reactive oxygen species have been implicated. The saliva contains protective antioxidants which statutorily curtail these destructive oxygen molecules. A functional compromise of the antioxidants may precipitate oxidative stress leading to the increased oral disease susceptibility. However, salivary markers of oxidative stress have not been sufficiently studied in the diabetics. Methods: A total of 166 adults were recruited for this study. They comprised of 95 Type 2 diabetic patients and 71 healthy non-diabetic controls. About 3 ml of unstimulated saliva samples were collected from participants and processed, levels of salivary H2O2, NO and MDA were measured using spectrophotometry method and compared between the two groups. Data was analysed using t-test, logistic regression and receiver operating characteristics (ROC) with statistical significance set at p<0.05. Results: Salivary H2O2 (p=0.024) and NO (p=0.002) were significantly higher in the diabetic patients when compared to the healthy non-diabetic control group. Binary logistic regression showed that patients with Type 2 diabetic mellitus are more likely to have elevated salivary H2O2 (OR= 1.013; p=0.025) and NO (OR=1.016; p=0.003) levels. ROC analysis showed statistically significant performance of salivary NO levels in distinguishing between T2DM patients and healthy controls. Conclusions: Higher levels of oxidative stress markers including salivary H2O2 and NO in the diabetic groups could be a pointer to the characteristic high prevalence of oral diseases in diabetes mellitus, given that oxidative stress predisposes to disease vulnerability. This calls for increased attention to oral health in diabetes management to minimise co-morbidity.

Markers of Oxidative Stress in the Saliva of Type 2 Diabetic Patients (Preprint)

2020 ◽  
Author(s):  
Olatunde Olayanju ◽  
Chika Juiet Okwor ◽  
Gabriel Nku Odok Jr ◽  
Gabriel Nku Odok Jr ◽  
Nnaemeka Elvis Awah ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Reactive Oxygen Species ◽  
Diabetic Patients ◽  
Oxygen Species ◽  
Oral Diseases ◽  
Type 2 Diabetic Patients ◽  
Markers Of Oxidative Stress ◽  
Type 2 Diabetic

BACKGROUND Diabetes mellitus is associated with increased prevalence of oral diseases. Reactive oxygen species have been implicated in the development of these diseases. The saliva contains a balanced proportion of protective antioxidants and reactive oxygen species, disrupting this balance favours disease development. However, level of salivary markers of oxidative stress has not been sufficiently studied in the diabetics. OBJECTIVE Thus, this study aimed to measure salivary H2O2, NO and MDA in diabetic patients in comparison to non-diabetic controls. METHODS A total of 166 adults comprising of 95 Type 2 diabetic patients and 71 healthy non-diabetic controls were recruited for this study. About 3 ml of unstimulated saliva samples were collected from participants after rinsing their mouth with clean water. Levels of H2O2, NO and MDA were measured in all saliva samples using spectrophotometry. Data was analysed using t-test, logistic regression and receiver operating characteristics (ROC) with statistical significance set at p<0.05. RESULTS Salivary H2O2 (p=0.024) and NO (p=0.002) were significantly higher in the diabetic patients when compared to the healthy non-diabetic control group. Binary logistic regression showed that patients with Type 2 diabetic mellitus are more likely to have elevated salivary H2O2 (OR= 1.013; p=0.025) and NO (OR=1.016; p=0.003) levels. ROC analysis showed statistically significant performance of salivary NO levels in distinguishing between T2DM patients and healthy controls. CONCLUSIONS Higher levels of salivary H2O2 and NO could be a pointer to the high prevalence of oral diseases in diabetes mellitus. This calls for increased attention to oral health in diabetes management.

Role of Oxidative Stress and Mitochondrial Dysfunction in Skeletal Muscle in Type 2 Diabetic Patients

2016 ◽  
Vol 22 (18) ◽  
pp. 2650-2656 ◽  
Author(s):  
Noelia Diaz-Morales ◽  
Susana Rovira-Llopis ◽  
Irene Escribano-Lopez ◽  
Celia Bañuls ◽  
Sandra Lopez-Domenech ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Mitochondrial Dysfunction ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetic

scholarly journals Soy Milk Consumption, Inflammation, Coagulation, and Oxidative Stress Among Type 2 Diabetic Patients With Nephropathy

Diabetes Care ◽  
2012 ◽  
Vol 35 (10) ◽  
pp. 1981-1985 ◽  
Author(s):  
M. S. Miraghajani ◽  
A. Esmaillzadeh ◽  
M. M. Najafabadi ◽  
M. Mirlohi ◽  
L. Azadbakht
Keyword(s):  
Oxidative Stress ◽  
Diabetic Patients ◽  
Milk Consumption ◽  
Soy Milk ◽  
Type 2 Diabetic Patients ◽  
And Oxidative Stress ◽  
Type 2 Diabetic

Awareness of Complications in Diabetic Patients - Study in a Tertiary Care Centre in Kerala

2021 ◽  
Vol 8 (37) ◽  
pp. 3334-3338
Author(s):  
Satish Kumar
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Tertiary Care ◽  
Diabetic Patients ◽  
Tertiary Care Centre ◽  
Type 2 Diabetic Patients ◽  
Complications Of Diabetes ◽  
Type 2 Diabetic

BACKGROUND Type 2 diabetes mellitus is common in the Indian population and awareness regarding the disease and its complications is low among the patients with diabetes. There is lack of studies regarding awareness of complications of diabetes among type 2 diabetic patients. The purpose of this study was to assess the awareness of diabetic complications among type 2 diabetic patients. METHODS This is an institutional based observational study. The sample comprised of 150 adult patients with type 2 diabetes mellitus who underwent treatment in the Department of Medicine, Medical College Kottayam and the patient’s age ranged from 18 - 77 years. RESULTS Majority of patients were in the age group 38 to 57 and the sample comprised of 56 % males and 44 % female patients. Out of 150 patients involved in the study, 120 patients (80 %) were aware of the complications of diabetes mellitus. Among the male patients, 70 (83.33 %) and among females, 50 (75.75 %) were aware of the complications in diabetes. In the study sample, 92 (61.33 %) were aware of the foot problems, 120 patients (80 %) were aware of renal complications, 91 patients were aware regarding eye problems of diabetes, 38 (25.33 %) regarding development of hypertension, 62 (41.33 %) regarding heart attack, 42 (28 %) patients were aware regarding development of stroke and 78 patients (52 %) had awareness regarding occurrence of recurrent infections. CONCLUSIONS Awareness regarding diabetes and its complications is fairly good among the diabetic patients in Kerala. Awareness regarding all complications of diabetes was higher among males than females. Providing awareness to type 2 diabetic patients by various educational programs may be of further help to prevent complications and to decrease the mortality and morbidity in type 2 diabetes patients. KEYWORDS Awareness, Diabetes

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