Mechanical Stability and Proteolytic Activity of Resin–dentin Bonds Using the Cross-linked Dry Bonding Technique

2021 ◽  
Author(s):  
M Citta ◽  
G Anovazzi ◽  
FG Basso ◽  
DLS Scheffel ◽  
J Zhou ◽  
...  
Keyword(s):  
Bond Strength ◽  
Proteolytic Activity ◽  
Mechanical Stability ◽  
Grape Seed ◽  
Cross Linking ◽  
Water Control ◽  
Group 4 ◽  
Etch And Rinse ◽  
The Cross ◽  
Group 2

SUMMARY Objective: To evaluate the mechanical stability and the proteolytic activity of bonds created by a two-step, etch-and-rinse adhesive applied to cross-linked and air-dried etched dentin. Methods: Flat dentin surfaces were produced in 64 extracted sound human molars. The dentin was etched with 35% phosphoric acid for 15 seconds, and then the teeth were divided into groups according to the cross-linking solution applied on the etched dentin. Group 1: 5% grape seed extract (GSE), Group 2: 5% glutaraldehyde, Group 3: Gluma Desensitizer, or Group 4: deionized water (control). Solutions were applied for 60 seconds, followed by rinse and blot drying. Then, the teeth were separated into two subgroups where the etched dentin was kept moist or air-dried. The adhesive was applied followed by a composite resin buildup. After 24 hours, the teeth were cut into beams (0.81 mm2) that were tested for microtensile strength immediately or after 12 months of aging in a 37°C saliva-like buffer. Additional teeth (n=32) were bonded as described and cut into 0.5-mm-thick slabs. The slabs were prepared for nanoleakage (scanning electron microscopy) and in situ zymography (EnzChek Protease Assay Kit). Bond strength data were submitted to ANOVA and Tukey tests (α=0.05). Results: Significant reduction in immediate bond strength (ca 65%) and increase in proteolytic activity was seen when the etched dentin was air dried without previous cross-linking biomodification. Conversely, bond strengths did not differ from those produced on wet dentin when collagen was cross-linked before air drying, irrespective of the solution applied. For both moist and air-dried etched dentin, collagen cross-linking resulted in mechanically stable bonds and reduced proteolytic activity after 12 months of storage. Conclusion: Bonds produced by the application of a two-step, etch-and-rinse adhesive to cross-linked, air-dried, etched dentin were mechanically stable and revealed reduced proteolytic activity after 1 year of aging.

scholarly journals The Effects of Prophylactic Ozone Pretreatment of Enamel on Shear Bond Strength of Orthodontic Brackets Bonded with Total or Self-Etch Adhesive Systems

2010 ◽  
Vol 04 (04) ◽  
pp. 367-373 ◽  
Author(s):  
Sevi Burcak Cehreli ◽  
Asli Guzey ◽  
Neslihan Arhun ◽  
Alev Cetinsahin ◽  
Bahtiyar Unver
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Orthophosphoric Acid ◽  
Ozone Treatment ◽  
Bond Failure ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Self Etch ◽  
Group 1

Objectives: The aim of this in vitro study is to determine (1) shear bond strength (SBS) of brackets bonded with self-etch and total-etch adhesive after ozone treatment (2) bond failure interface using a modified Adhesive Remnant Index (ARI).Methods: 52 premolars were randomly assigned into four groups (n=13) and received the following treatments: Group 1: 30 s Ozone (Biozonix, Ozonytron, Vehos Medikal, Ankara, Turkey) application + Transbond Plus Self-Etching Primer (SEP) (3M) + Transbond XT (3M), Group 2: Transbond Plus SEP + Transbond XT, Group 3: 30 s Ozone application + 37% orthophosphoric acid + Transbond XT Primer (3M) + Transbond XT, Group 4: 37% orthophosphoric acid + Transbond XT Primer + Transbond XT. All samples were stored in deionised water at 37oC for 24 hours. Shear debonding test was performed by applying a vertical force to the base of the bracket at a cross-head speed of 1 mm/min.Results: The mean SBS results were Group 1: 10.48 MPa; Group 2: 8.89 MPa; Group 3: 9.41 MPa; Group 4: 9.82 MPa. One-Way Variance Test revealed that the difference between the groups was not statistically significant (P=0.267). Debonded brackets were examined by an optical microscope at X16 magnification to determine the bond failure interface using a modified ARI. The results were (mean) Group 1: 2.38; Group 2: 1.31; Group 3: 3.00; Group 4: 1.92. Multiple comparisons showed that Groups 1 and 2, 2 and 3, 3 and 4 were statistically different (P=0.014, P<.001 and P=0.025).Conclusions: Ozone treatment prior to bracket bonding does not affect the shear bond strength. (Eur J Dent 2010;4:367-373)

scholarly journals Evaluation of shear bond strength of composite to porcelain according to surface treatment

2003 ◽  
Vol 14 (2) ◽  
pp. 132-135 ◽  
Author(s):  
Claudia Minami Kussano ◽  
Gerson Bonfante ◽  
José Gilmar Batista ◽  
João Henrique Nogueira Pinto
Keyword(s):  
Bond Strength ◽  
Surface Treatment ◽  
Hydrofluoric Acid ◽  
Shear Bond Strength ◽  
Room Temperature ◽  
Force Load ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

This study evaluated the shear bond strength of porcelain/composite using 40 metal + porcelain + composite cylindrical specimens divided into 4 groups, according to porcelain surface treatment: 1) no treatment, 2) mechanical retentions performed with diamond burs, 3) etching with phosphoric acid+silane, and 4) etching with hydrofluoric acid+silane. After being stored in distilled water at room temperature for one week, the specimens were submitted to a shear force (load) and the data were analyzed statistically (ANOVA). The means (in Mpa) of the groups were: 4.71 (group 1); 4.81 (group 2); 11.76 (group 3); 11.07 (group 4). There were no statistically significant differences between groups 1 and 2 and between groups 3 and 4.

Dentin Bond Strength of Composites with Self-etching Adhesives Using LED Curing Lights

2007 ◽  
Vol 8 (5) ◽  
pp. 34-42 ◽  
Author(s):  
Yonca Korkmaz ◽  
Nuray Attar
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Significance Level ◽  
Group 4 ◽  
Group 6 ◽  
Group 3 ◽  
Curing Light ◽  
Group 5 ◽  
Group 2 ◽  
Self Etch

Abstract Aim The purpose of this study was to investigate the effect of light-emitting diode (LED) light curing units (LCUs) compared with halogen LCUs on the shear bond strength (SBS) of one nanofill composite (Filtek Supreme) and one microhibrid composite (Artemis) with self-etch adhesives. Methods and Materials The buccal surfaces of 60 non-carious extracted human molars were flattened to expose dentin and, subsequently, polished for 60 seconds with 600-grit wet silicon carbide abrasive paper. Specimens were assigned into six groups (n=10) according to composite material, self-etch adhesive, and curing light used as follows: Group 1: Adper Prompt L-Pop (AP) and Filtek Supreme (FS) using an Elipar Free Light (EFL); Group 2: AP and FS using an Elipar Free Light 2 (EFL2); Group 3: AP and FS using a Hilux Expert (HE) light, Group 4: AdheSE (AS)+Artemis (AR) using an EFL; Group 5: AS+AR using an EFL2; and Group 6: AS+AR using a HE light. The specimens were thermocycled for 500 cycles (5°C–55°C) and then loaded to failure in a Zwick universal testing machine at a crosshead speed of 5 mm/minute. SBS values were calculated as megapascals (MPa) and statistically analyzed using the one-way analysis of variance (ANOVA) test at a significance level of 0.05. Results Mean SBS (± standard deviations) values were as follows: Group1: 15.99±5.18; Group 2: 18.76±6.71; Group 3: 17.70±5.04; Group 4: 16.93±3.99; Group 5: 18.01±5.19, and Group 6: 17.46±5.40. There were no statistically significant differences for SBS to dentin among the groups tested. Conclusion The LED curing lights used in the study seem to be comparable with the halogen curing light for nanofill and microhybrid composites used in conjunction with self-etching systems in dentin. The EFL2 reduces curing time, which can be considered as an advantage. Citation Korkmaz Y, Attar N. Dentin Bond Strength of Composites with Self-etching Adhesives Using LED Curing Lights. J Contemp Dent Pract 2007 July;(8)5:034-042.

Effects of Surface Texture and Etching Time on Roughness and Bond Strength to Ground Enamel

2009 ◽  
Vol 10 (4) ◽  
pp. 17-25 ◽  
Author(s):  
Andre Figueiredo Reis ◽  
Flávio Henrique Baggio Aguiar ◽  
Marcelo Giannini ◽  
Patricia Nóbrega Rodrigues Pereira
Keyword(s):  
Bond Strength ◽  
Surface Texture ◽  
Etching Time ◽  
Ground Enamel ◽  
Group 4 ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Group 10 ◽  
Group 5 ◽  
Group 1

Abstract Aim The aim of this study was to assess the influence of surface texture and etching technique on surface roughness (Ra) and bond strength (BS) to enamel and to determine if a correlation exists between them. Methods and Materials Fifty enamel blocks were either roughened with 600-grit SiC paper or polished with diamond pastes. After establishing ten test groups (n=5), the initial Ra measurements, rough (R) and smooth (S) enamel surfaces were etched according to the following protocols: Group 1(R)/Group 2(S)- 35% phosphoric acid gel (H3PO4) for 15 seconds; Group 3(R)/Group 4(S)- 35% H3PO4 for 60 seconds; Group 5(R)/Group 6(S)- Clearfil SE Bond primer for 20 seconds; Group 7(R)/Group 8(S)- self-etching primer (SEP) for 60 seconds; Group 9(R)/ Group 10(S)- 35% H3PO4 for 15 seconds + SEP for 20 seconds. After treatments, a new Ra measurement was performed and enamel surfaces were bonded with either Single Bond (Group 1 to Group 4) or Clearfil SE Bond (Group 5 to Group 10). Afterwards, specimens were prepared for the microtensile test. Ra values were analyzed using repeated-measures analysis of variance (ANOVA) and the BS values were analyzed using a two-way ANOVA and Tukey test (5%). Correlation between BS and Ra values was assessed using the Pearson's test. Results The application of SEP produced the lowest Ra values. No significant difference was detected between the BS values of polished and rough surfaces. No correlation was observed between Ra and BS values. Even though etching enamel with the SEP resulted in a surface with less roughness, similar BS values were observed for both self-etching and etch-and-rinse techniques. Conclusion Within the limits of this study increasing the etching time or combining both etching techniques failed to improve the BS using SEP or etch-and-rinse systems. Clinical Significance Based on the findings of this study, there is no clinical justification for increasing the etching time or for combining the use of a SEP following the use of a 35% H3PO4 etchant to achieve a greater BS to ground enamel. Citation Reis AF, Aguiar FHB, Pereira PNR, Giannini M. Effects of Surface Texture and Etching Time on Roughness and Bond Strength to Ground Enamel. J Contemp Dent Pract 2009 July; (10)4:017-025.

scholarly journals A Comparative Evaluation of Three Commercially Available Dentin Desensitizers on the Shear Bond Strength of Composite Resins: An in vitro Study

2015 ◽  
Vol 5 (2) ◽  
pp. 65-68
Author(s):  
Shruti Patil ◽  
Prajna Shetty
Keyword(s):  
Bond Strength ◽  
Comparative Evaluation ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Composite Resins ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

ABSTRACT Background The dentin desensitizers available for in-office application for prevention and/or treatment of post restorative dentinal hypersensitivity, act by blocking the open dentinal tubules. In doing so, they may influence the bond strength of the restorative resins. Thus, the aim of the study was to evaluate the effect of desensitizers on the shear bond strength of dentin adhesives and to check the extent of dentinal tubule occlusion caused by these desensitizers. Materials and Methods Sixty-four premolars were randomly divided into four groups of sixteen each. The middle depth dentin was exposed by on the buccal surface and was etched using 37% phosphoric acid and rinsed. They were assigned to, Group 1: Gluma desensitizer, Group 2: Vivasens desensitizer, Group 3: Gluma Comfort Bond and desensitizer, and Group 4: Adper Single Bond 2. Composite resin post of 2 mm in height and width were built on these specimens. The samples were then mounted in acrylic resin blocks. Universal testing machine was used to test the shear bond strength. The data were analyzed using one-way analysis of variance (ANOVA) and post hoc Tukey's test. Results The mean values of the shear bond strength were for: Group 1—18.61 ± 1.03 MPa, Group 2—17.53 ± 1.36 MPa, Group 3—22.63 ± 1.61 MPa and Group 4—23.12 ± 1.02 MPa. There was a statistically significant difference (p < 0.05) among the groups. Conclusion The use of dentin desensitizers’ influences bond strength between the tooth and the adhesive. Among the various agents, the single bottle system containing Gluma comfort bond and desensitizer had the best adhesion among the desensitizer groups. How to cite this article Patil S, Naik B, Shetty P. A Comparative Evaluation of Three Commercially Available Dentin Desensitizers on the Shear Bond Strength of Composite Resins: An in vitro Study. J Contemp Dent 2015;5(2):65-68.

scholarly journals Does surface treatment of demineralized dentin impact on shear bond strength?

2021 ◽  
Vol 6 (2) ◽  
pp. 023-028
Author(s):  
Fatemeh Rezaei ◽  
Atefeh Nemati-Karimooy ◽  
Horieh Moosavi
Keyword(s):  
Bond Strength ◽  
Laser Irradiation ◽  
Shear Bond Strength ◽  
Surface Treatments ◽  
Universal Adhesive ◽  
Yag Laser ◽  
Group 4 ◽  
Human Dentin ◽  
Group 2 ◽  
Demineralized Dentin

Aim: This study investigated the effect of various surface treatments on the bond strength of a universal adhesive to demineralized dentin. Materials and Methods: Human dentin surfaces of forty-eight carries-free maxillary premolars were prepared and demineralized by acidic solutions. Teeth were randomly distributed into four groups (n=12) getting different surface treatments. Three minutes application of a CPP‑ACP (group 1), Er: YAG laser irradiation (group 2), Er: YAG laser irradiation combined with three minutes application of CPP‑ACP (group 3), no treatment (group 4). Thereafter composite tube (Filtek TM Ultimate, 3M ESPE) bonded to the testing surfaces via universal adhesive; Single Bond Universal and the Shear Bond Strength (SBS) measured. Data were analyzed utilizing ANOVA and Tukey tests (P<0.05). Results: Only application of CPP-ACP and Er: YAG laser radiation, resulted in the highest and lowest SBS respectively (p<0.001). Bond strength after irradiation of Er: YAG laser combined with three minutes application of CPP‑ACP was a little more than laser alone. Conclusion: The application of CPP-ACP could increase the SBS of universal adhesives used to demineralized dentin. Laser irradiation application has an adverse effect on SBS.

scholarly journals Effect of the superficial treatment in the repair of silorane-based composite aged artificially

2015 ◽  
Vol 63 (4) ◽  
pp. 376-382
Author(s):  
Chaiane Emilia DALAZEN ◽  
Mario Alexandre Coelho SINHORETI ◽  
Margareth COUTINHO
Keyword(s):  
Bond Strength ◽  
Aluminum Oxide ◽  
Thermal Cycling ◽  
Surface Treatments ◽  
Control Group ◽  
Group 4 ◽  
Diamond Bur ◽  
Composite Methods ◽  
Group 3 ◽  
Group 2

Objective: To evaluate the effects of different surface treatments, in repair bond strength of an aged silorane-based composite. Methods: Specimens were made of a silorane-based composite (P90, 3M/ESPE), aged with thermal-cycling (10,000 cycles; 5-55ºC) and randomly submitted to different surface treatments (each group n = 24): control group (no repair); group 1 (only application of P90 adhesive); group 2 (diamond bur asperization + P90 adhesive); group 3 (aluminum oxide sandblasting + P90 adhesive) and group 4 (asperization + sandblasting + P90 adhesive). Then, the specimens were repaired with the same silorane-based composite and each group randomly subdivided into 2 subgroups: stored in distilled water at 37 °C for 7 days or submitted to thermal-cycling (5,000 cycles). After aging, the tensile test was carried out. The data was submitted to 2-way analysis of variance (2-way ANOVA). Results: The repaired groups aged with oven storage for 7 days did not statistically differ (p>0.05) from the corresponding groups aged with thermal-cycling. The bond strength of the specimens was significantly influenced by the superficial treatment (p<0.01). The highest values of tensile bond strength were found in groups 3 (20.76 ± 4.51 MPa) and 4 (20.67± 3.96 MPa), regardless of aging and repair. Conclusion: The repair of silorane-based composite showed to be stable after aging, and the superficial treatment with aluminum oxide sandblasting was the most effective.

scholarly journals Comparison of Shear Bond Strength, Adhesive Remnant Index and Enamel Cracks in Bonding and Rebonding of Stainless Steel Brackets to Enamel Surface Conditioned with Er:YAG Laser Versus Conventional Acid Etching

2021 ◽  
Author(s):  
Elahe Soltanmohamadi Borujeni ◽  
Javad Chalipa ◽  
Ardavan Etemadi ◽  
Maryam Nasiri ◽  
Mohammad Javad Kharrazifard ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Er:Yag Laser ◽  
Yttrium Aluminium Garnet ◽  
Acid Etching ◽  
Group 4 ◽  
Laser Conditioning ◽  
Adhesive Remnant Index ◽  
Group 3 ◽  
Group 2

Objectives: The aim of the present study was to compare shear bond strength (SBS), adhesive remnant index and enamel cracks in bonding and rebonding of brackets to enamel, conditioned with erbium-doped yttrium aluminium garnet (Er:YAG) laser and conventional acid-etching. Materials and Methods: Fifty-two bovine lower incisors were randomly divided into four groups consisting of group 1 (acid-conditioning in both bondings), group 2 (acid-conditioning in first and laser-conditioning in second bonding), group 3 (laser-conditioning in first- and acid-conditioning in second bonding), and group 4 (laser-conditioning in both bondings). After bracket placement, the samples were thermocycled and tested for SBS in both bonding procedures. Adhesive remnant index scores and enamel cracks were also determined. Tukey's test and one-way analysis of variance was used for statistical analysis (P<0.05). Results: Mean SBS in the first bonding was 23.59MPa in groups 1 and 2, and 6.9MPa in groups 3 and 4. (P<0.001). The acid-etched teeth had a significantly lower SBS in rebonding, regardless of the reconditioning method (P<0.001). The SBS of the teeth conditioned with Er:YAG laser in the first bonding did not show significant changes in rebonding, although mean SBS was higher compared to the first bonding (P=0.675). Bonding most often failed at the enamel-adhesive interface and enamel cracks were observed in a few teeth. Conclusion: The method of primary enamel preparation can affect SBS in rebonding. Based on our results, the mean SBS of Er:YAG-conditioned groups was clinically acceptable in bonding and rebonding, although it was lower compared to the acidetched samples.

scholarly journals Long-term bond strength of a self-adhesive resin cement to intraradicular dentin pretreated with chlorhexidine and ethanol

2017 ◽  
Vol 46 (2) ◽  
pp. 97-103
Author(s):  
Mariah Carvalho Guimarães dos SANTOS ◽  
Flávia Lucisano Botelho AMARAL ◽  
Cecília Pedroso TURSSI ◽  
Roberta Tarkany BASTING ◽  
Fabiana Mantovani Gomes FRANÇA
Keyword(s):  
Treatment Group ◽  
Bond Strength ◽  
Resin Cement ◽  
Tooth Surface ◽  
Adhesive Resin ◽  
Group 4 ◽  
Adhesive Resin Cement ◽  
Group 2 ◽  
Push Out

Abstract Introduction Self-adhesive resin cements do not require prior preparation of the tooth surface, therefore dentin pretreatments may influence long-term bond strength. Objective To evaluate the influence of 100% ethanol (ET) and 2% chlorhexidine (CL) treatment of intraradicular dentin on the long-term bond strength (BS) of a self-adhesive resin cement (SRC). Material and method 80 bovine roots were restored with fiber posts and SRC (U200 3M/ESPE) and distributed into 4 groups according to dentin treatment: Group 1 – without treatment; Group 2 – 2% CL for 1 minute; Group 3 – 100% ET for 1 minute; Group 4 – 2% CL, followed by 100% ET. The samples were cross-sectioned to obtain two sections (0.7 mm) thick for each root third: coronal, middle and apical. The immediate push-out test was carried out after 48 hours, and the long-term push-out test, after 180 days. Result The three-way ANOVA test for randomized blocks showed no difference between the BS values at 48 hours and 80 days, irrespective of the treatment and the third (p>0.05). The interaction of the treatment/third pairing was significant (p = 0.041) since the treatment with CL promoted lower BS in the coronal third, while treatment with ET promoted better BS in the apical third. Conclusion Treatment with CL and ET, separately or combined, promoted no differences between the BS values of the SRC to root dentin over time.

Effect of Klebsiella pneumoniae on speck disease development in Solanum lycopersicum

2017 ◽  
Vol 51 (05) ◽  
Author(s):  
Indu Gaur ◽  
P. D. Sharma ◽  
P. K. Paul
Keyword(s):  
Klebsiella Pneumoniae ◽  
Solanum Lycopersicum ◽  
Pseudomonas Syringae ◽  
Disease Incidence ◽  
Post Inoculation ◽  
Water Control ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Aseptic Conditions

Colonisation and infection of Pseudomonas syringae pv. tomato is governed not only by host resistance but also by its interactions with other phylloplane bacterial colonizers. In the present study, reduced disease symptoms were observed as a result of interaction between P. syringae pv. tomato and Klebsiella pneumoniae, a predominant human enteric pathogen colonising phyllosphere. Tomato (Solanum lycopersicum) plants raised under controlled aseptic conditions were divided into 4 groups of 25 plants each and treated as follows: Group 1- inoculated with P. syringae pv. tomato; Group 2- inoculated with K. pneumoniae; Group 3- combination of P. syringae pv. tomato and K. pneumoniae (1:1); Group 4- Distilled water (control). Sampled leaves from the above groups were analysed at 24, 48, 72 and 96 hours post inoculation for the activity of cytoplasmic Peroxidase (POX), Polyphenoloxidase (PPO) and Phenylalanine ammonia lyase (PAL). Disease incidence was recorded in treated as well as leaves emerging after 2 weeks of inoculation. Plants treated with P. syringae pv. tomato had maximum disease incidence while no infection was observed in control and those inoculated with K. pneumoniae alone. Inoculation of plants with combination of both microbes could substantially reduce the disease severity. The results thus demonstrate that K. pneumoniae, a common HEP on plant surfaces, reduces the ability of P. syringae pv. tomato to colonise the host.

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