scholarly journals AKTIVITAS INSEKTISIDA EKSTRAK DAUN DAN BIJI TEPHROSIA VOGELII J. D. HOOKER (LEGUMINOSAE) DAN EKSTRAK BUAH PIPER CUBEBA L. (PIPERACEAE) TERHADAP LARVA CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: CRAMBIDAE)

2010 ◽  
Vol 10 (1) ◽  
pp. 1-12
Author(s):  
Muhamad Abizar ◽  
Djoko Prijono
Keyword(s):  
Ethyl Acetate ◽  
Insecticidal Activity ◽  
Leaf Extract ◽  
Seed Extract ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
Feeding Method ◽  
Tephrosia Vogelii ◽  
Leaf Residue ◽  
Seed Extracts

Insecticidal activity of leaf and seed extracts of Tephrosia vogelii J. D. Hooker (Leguminosae) and fruit extract of Piper cubeba L. (Piperaceae) on the cabbage head caterpillar, Crocidolomia pavonana (L.) (Lepidoptera: Crambidae).  Ethyl acetate leaf and seed extracts of Tephrosia vogelii and a solid fraction of ethyl acetate fruit extract of Piper cubeba were evaluated for their insecticidal activity on second-instar larvae Crocidolomia pavonana by a leaf-residue feeding method in the laboratory.  Leaf extracts of purple and white-flowered T. vogelii showed the same pattern of component separation on silica gel TLC plate (Rf between 0.21 and 0.94), and likewise the separation of components of seed extracts of purple and white-flowered T. vogelii (Rf between 0.31 and 0.96).  All four kinds of T. vogelii extracts showed intense UV-absorbing nonpolar spots (Rf > 0.8).  Based on LC50 ratio at day 4, leaf extract of purple-flowered T. vogelii (LC50 0.075%) was 4.30, 2.70, 2.21, and 1.64 times more toxic than fruit extract of P. cubeba, seed extract of white-flowered T. vogelii, seed extract of purple-flowered T. vogelii, and leaf extract of white-flowered T. vogelii, respectively.  All T. vogelii extracts were more toxic to C. pavonana larvae than P. cubeba fruit extract.  At LC95 level, a mixture of leaf extract of purple-flowered T. vogelii and fruit extract of P. cubeba (5:9, w/w) was more toxic to C. pavonana larvae than each extract tested separately.  This extract mixture had synergistic joint action against C. pavonana larvae both at LC50 and LC95 level.  Thus, leaf extract of purple-flowered T. vogelii and its mixture with P. cubeba fruit extract are promising to be used for controlling C. pavonana.

scholarly journals Insecticidal and repellent activities of the chloroform extracts of Urena sinuata L. against Tribolium castaneum (Herbst) adults

2012 ◽  
Vol 30 ◽  
pp. 25-28 ◽  
Author(s):  
M Abdullah ◽  
A Kumar Pk ◽  
DKMA Saleh ◽  
A R Khan ◽  
N Islam
Keyword(s):  
Tribolium Castaneum ◽  
Insecticidal Activity ◽  
Leaf Extract ◽  
Red Flour Beetle ◽  
Repellent Activity ◽  
Root Extract ◽  
High Toxicity ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
Stem Extract

The insecticidal and repellent activity tests of the chloroform extracts of fruit, leaf, root and stem of Urena sinuata L. against the red flour beetle Tribolium castaneum L. adults have been conducted. The leaf, root and stem extracts showed high toxicity by giving mortality of the beetles within 30 min. of exposure. The LD50 values for the 12, 24, 36 and 48h of exposures were 0.354, 0.262, 0.209 and 0.196mg cm2 for the fruit extract; 0.587, 0.413, 0.355 and 0.299mg cm2 for the leaf extract; 0.440, 0.389, 0.340 and 0.268mg cm2 for the root extract and 0.968, 0.661, 0.491 and 0.362mg cm2 for the stem extract. The insecticidal activity can be arranged in the order of fruit> root> leaf> stem extracts. The root and stem showed repellent activity against T. castaneum adults at P<0.01 and P<0.05 levels of significance, while the fruit and the leaf extracts did not show repellency at all. DOI: http://dx.doi.org/10.3329/ujzru.v30i0.10740 Univ. j. zool. Rajshahi Univ. Vol. 30, 2011 pp. 25-28

scholarly journals Nymphicidal effect of vegetal extracts of Annona mucosa and Anonna crassiflora (Magnoliales, Annonaceae) against rice stalk stink bug, Tibraca limbativentris (Hemiptera, Pentatomidae)

2014 ◽  
Vol 36 (spe1) ◽  
pp. 217-224 ◽  
Author(s):  
Diones Krinski ◽  
Angélica Massaroli
Keyword(s):  
Insecticidal Activity ◽  
Leaf Extract ◽  
Statistical Significance ◽  
Seed Extract ◽  
Stink Bug ◽  
Bed Bug ◽  
Small Producers ◽  
Chloroform Methanol ◽  
Seed Extracts

This study aimed to verify the chloroform-methanol nymphicidal action of extracts of Annona mucosa leaves and seeds and of A. crassiflora seeds on second instar nymphs of rice stalk stink bug, Tibraca limbativentris. For each extract the concentrations of 0.5%, 1.0%, 2.0%, 4.0%, 8.0%, and two control treatments (water and Tween80®) were used. The results show that the seed extracts of A. mucosa and A. crassiflora have insecticidal activity against the T. limbativentris nymphs with statistical significance for all concentrations when compared with controls. The seed extract of A. mucosa showed the higher toxicity with greater than 75% mortality at a concentration of 1.0% in the first 24 h after application. The leaf extract of A. mucosa presented the lowest toxicity with no more than 40% mortality. The seed extract of A. crassiflora showed intermediate toxicity among all the tested extracts, and the nymph's mortality exceeded 80% for the highest concentration after 120 h of application. Considering these results, we were able to observe that the seeds extract of A. mucosa may be an alternative for the control of bed bug nymphs T. limbatriventris, especially for small producers.

scholarly journals Effects of cypermethrin alone and in combination with leaf and seed extracts of neem against adult Tribolium castaneum (Herbst)

1970 ◽  
Vol 25 ◽  
pp. 45-49 ◽  
Author(s):  
M Khalequzzaman ◽  
Makhsuda Khanom
Keyword(s):  
Synergistic Effect ◽  
Ethyl Acetate ◽  
Tribolium Castaneum ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Local Strain ◽  
Seed Extract ◽  
Red Flour Beetle ◽  
Insignificant Effect ◽  
Seed Extracts

Cypermethrin was applied either alone or in combination with leaf and seed extracts of neem (Azadirachta indica A. Juss) to four strains of the adult red flour beetle Tribolium castaneum (Herbst) and the mortality was assessed after 24 and 48 hrs of treatments. The LD50 values were calculated as 0.0072, 0.0105, 0.0056 and 0.0124 μgcm-2 after 24 hours and 0.0041, 0.0076, 0.0046 and 0.0097 μgcm-2 after 48 hours of treatment for the Local, CR-1, FSS-II and CTC-12 strains respectively. The results showed synergism when applied in combined doses of cypermethrin and leaf and seed extracts with petroleum spirit, ethyl acetate and methanol. Leaf extract in acetone had an insignificant effect showing its inertness with cypermethrin excepting 24 hrs after treatment of Local strain and 48 hrs after treatment of CTC-12 strain. Seed extract in methanol in Local strain showed insignificant effect after 24 hrs of treatment but a synergistic effect occurred after 48 hrs of treatment. Key words: Cypermethrin, Azadirachta indica, Tribolium castaneum, synergism Univ. j. zool. Rajshahi Univ. Vol.25 2006 p.45-40

scholarly journals Phytochemical analysis, anti-oxidant and anti-microbial activity of Acalypha indica leaf extracts in different organic solvents

2016 ◽  
Vol 8 (3) ◽  
pp. 444 ◽  
Author(s):  
Chekuri Sudhakar ◽  
Narendar Vankudothu ◽  
Shivaprasad Panjala ◽  
Nirmala Babu Rao ◽  
Roja Rani Anupalli
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Microbial Activity ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Anti Oxidant ◽  
Acalypha Indica

The herb <em>Acalypha indica </em>which belongs to Euphorbiaceae family has multiple medicinal properties which include anti-oxidant, anti-bacterial, anti-fungal, anti-inflammatory, antiulcer, anti-helmintic, anti-cancerous, anti-venom, and neuro-protective activity. The present study was designed to evaluate the phytochemical, antimicrobial and anti-oxidant activity of <em>Acalypha indica </em>leaves extracts in different solvent extractions like methanol, hexane, ethyl acetate and petroleum ether. Fresh leaves of the plant were collected and shade dried. Dried leaves were milled to obtain powder. Powder was subjected to soxhlet extraction using solvents and extracts were successively obtained. Phytochemical analysis was conducted following standard methods.  Phytochemical analysis showed the presence of Alkaloids, Phenols, Saponins, Flavanoids and Amino acids. Leaf extract of methonal have shown the highest anti-oxidation capacity than hexane, ethyl acetate and petroleum ether. Anti-microbial activity has been performed on microbes like <em>Bacillus sps</em>, <em>E.coli,</em> <em>Psuedomonas sps</em> and <em>Streptococcus sps.</em> A highest value of zone of inhibition was found in methanol extract against <em>E.coli.</em> These results provide evidence that <em>Acalypha indica </em>leaf extract possesses vital phytochemicals, antimicrobial and antioxidant properties. Hence this plant can be studied further for drug analysis for finding potent medicines for diseases.

scholarly journals ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE)

2017 ◽  
Vol 9 (10) ◽  
pp. 265 ◽  
Author(s):  
Prashith Kekuda T. R. ◽  
Raghavendra H. L. ◽  
Shilpa M. ◽  
Pushpavathi D. ◽  
Tejaswini Petkar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Insecticidal Activity ◽  
Leaf Extract ◽  
Antiradical Activity ◽  
Radical Scavenging ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Ic50 Value ◽  
Agar Well Diffusion Assay ◽  
Dose Dependent

Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae).Methods: The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti.Results: Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical’s dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.

scholarly journals Extraction of the Metabolites from Medicinal plant Euphorbia leaf

2021 ◽  
Vol 37 (5) ◽  
pp. 1257-1261
Author(s):  
Uday M. Muddapur ◽  
Aejaz A. Khan ◽  
Amal Bahafi ◽  
S. M. Shakeel Iqubal ◽  
Abdul Rahman Ikbal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Research Work ◽  
Leaf Extracts ◽  
Different Types ◽  
Phytochemical Contents ◽  
Ethyl Acetate Extracts

The antimicrobial, phytochemical contents of extracts obtained from the leaf extract of mature Euphorbia pilulifera (E. pilulifera) were examined in this research work. Using ethyl acetate extracts of the leaves of Euphorbia pilulifera, phytochemical metabolites were studied. Further using chromatography, different components were separated and its antimicrobial activity was studied. Different types of bacterial organisms were used for evaluating the antimicrobial activity. The results indicated that leaf extracts are more sensitive towards organisms. Thus the presence of phytochemicals in the leaf extract can be used for the treatment of different diseases.

Antimicrobial potentials, phytochemical screening and determination of bioactive compounds from Three Indian folk medicinal plants

2020 ◽  
Vol 16 ◽  
Author(s):  
V. Vanitha ◽  
S. Vijayakumar ◽  
S. Rajalakshmi
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Ft Ir

Aim: To investigate the antimicrobial efficacy of various solvents leaf extracts of Aegle marmelos, Plumbago zeylanica and Rhinacanthus nasutus against human pathogenic organisms such as Acinetobacter baumannii, Salmonella enteriditis, Bacillus cereus, Streptococcus pneumonia, Mycobacterium tuberculosis and Aspergillus fumigatous by agar well diffusion method. Background: Also, this is the first time report on MIC and MBC/MFC activities and identify the phytochemicals and functional groups using HPLC, GC-MS and FT-IR analysis. Objective: Minimum inhibitory concentration (MIC), minimum bactericidal (MBC/MFC) values were also carried out by the micro dilution method. Methods: Ethyl acetate extract of P. zeylanica showed better antimicrobial activity against the tested pathogens, especially for S. pneumoniae (40 mm) followed by ethyl acetate extract of R. nasutus (36 mm) against S. pneumoniae. While least inhibition was observed for aqueous extract of P.zeylanica against S. enteritis (10 mm). Results: The MIC ranged from 3.75 µg/ml to 60µg/ml and MBC/MFC 7.5 µg/ml to 60 µg/ml. Phytochemical analysis exhibited the presence of alkaloids, steroids, saponins, flavonoids, tannins, terpenes, phenolics and cardiac glycoside in all samples of selected plant extracts. Tannins, phenolics and glycoside were found only in the ethyl acetate extract of P.zeylanica. HPLC and GC-MS analysis of ethyl acetate leaf extract of P. zeylanica exposed ten major peaks and eleven compounds respectively. Chemical nature of the leaf extract was analysed by FT-IR spectrum. Conclusion: Ethyl acetate leaf extract of P. zeylanica showed better antimicrobial activity than other extracts as well as standard drugs. Therefore, this plant could be studied in detail for the identification of active biomolecules for antimicrobial agents.

scholarly journals Effect of Botanical Extract on Pest Control in Brinjal Field

2013 ◽  
Vol 5 (2) ◽  
pp. 173-176
Author(s):  
MAK Azad ◽  
MN Yesmin ◽  
MS Islam
Keyword(s):  
Pest Control ◽  
Leaf Extract ◽  
Seed Extract ◽  
Normal Plant ◽  
Aegle Marmelos ◽  
Leaf Extracts ◽  
Botanical Extracts ◽  
Ficus Hispida ◽  
Botanical Extract ◽  
Swietenia Mahagoni

An experiment was conducted to evaluate the effect of eight botanical extracts on pest control in brinjal field. Accordingly, water extracts of dried leaves of Khuksa (Ficus hispida), Chotra (Lantana sp.), Chirata (Swietia chrata), Neem (Azadiracta indica), Bael (Aegle marmelos), Holde-hurhuri (Cleomp viscosa) and Marigold (Targetes erecta) and seeds of Mahogany (Swietenia mahagoni) were prepared and sprayed in experimental brinjal field at Rajshahi University. Out of these botanicals, Khuksha leaves extract showed best performance against the pest attack compare to other extracts. Marigold leaf extract also showed good performance in the protection of brinjal plant from pest. The efficacy of Chotra, and Chirata leaf extracts was nearly same in brinjal plot against the pest attack. Neem leaf extracts showed moderate performance against pest. Mahogany seed extract showed lowest efficacy and hampered the normal plant growth and caused fruit rottening as well as reduced the yield of brinjal. Although Khuksha, Beal and Marigold leaf extracts were found effective against brinjal pests but a higher production was observed in the treatments of Neem and Chirata leaf extracts in experimental brinjal field.DOI: http://dx.doi.org/10.3329/jesnr.v5i2.14809 J. Environ. Sci. & Natural Resources, 5(2): 173-176 2012

scholarly journals Acetylcholinesterase Inhibitors from Croton sylvaticus Ethyl Acetate Leaf Extract and their Mutagenic Effects

2013 ◽  
Vol 8 (6) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Mutalib A. Aderogba ◽  
Ashwell R. Ndhlala ◽  
Johannes Van Staden
Keyword(s):  
Ethyl Acetate ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Acetylcholinesterase Inhibitors ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Leaf Extracts ◽  
2D Nmr Spectroscopy ◽  
Mutagenic Effects

Croton sylvaticus is a medicinal plant widely employed in the management of inflammatory conditions, infections and oxidative stress related diseases such as insomnia, abdominal and internal inflammations. The activities demonstrated by the plant extracts support that its constituents possess the ability to reduce oxidative damage to body cells. Repeated column fractionation of the ethyl acetate fraction of a 20% aqueous methanol leaf extract using acetylcholinesterase (AChE) activity directed on Sephadex LH-20 afforded three compounds identified by mass spectrometry, and 1D and 2D NMR spectroscopy as: 2′-(3″,4″-dihydroxyphenyl)-ethyl-4-hydroxybenzoate (1), 3, 3′, 4′, 5, 7-pentahydroxyflavone (quercetin) (2), and 3, 4′, 5, 7-tetrahydroxyflavone (kaempferol) (3). These compounds are reported from the leaf extracts of C. sylvaticus for the first time and compound 1 is a new natural product. The microplate technique was used to determine the inhibitory effects of the plant extracts (crude and solvent fractions) and isolated compounds against AChE. The IC50 values of the compounds ranged from 60.7 to 415.0 μg/mL, with compound 2 having the best inhibitory activity; the IC50 values of the plant crude extract and solvent fractions ranged from 235.0 to 4695.0 μg/mL. The ethyl acetate fraction had the best inhibitory activity (IC50=235.0 μg/mL). The three compounds showed no mutagenic effects against Salmonella typhimurium tester strains TA98 and TA100. The activity demonstrated by these compounds suggests that they could be effective in the management of neurodegenerative disorders.

scholarly journals Evaluation of the phenolic compounds and the antioxidant potentials of Vitex agnus-castus L. leaves and fruits

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ceren Kımna ◽  
Tuğçe Fafal
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Antioxidant Agents ◽  
Agnus Castus ◽  
Chaste Tree

Abstract Objectives In this study, we aim at deciphering the phenolic content of Vitex agnus-castus L. leaf and fruit extracts prepared with different methods and relate it to their antioxidant activity. Methods In this study, phenolic compounds and the antioxidant potential of the ethanol fruit and leaf extracts of V. agnus-castus L. (Chaste tree) were evaluated spectrometrically. Furthermore, selected polyphenols, i.e., chlorogenic acid and rutin, were determined by the HPLC-DAD method qualitatively and quantitatively. Results The results obtained from leaf and fruit extracts were compared with a commercial product (CP) containing the fruit extract of V. agnus-castus. Leaf extract was found to be richer in flavonoids when compared to the fruit counterparts. Accordingly, they also showed higher antioxidant activity. Conclusions Extracts prepared here can be considered as promising antioxidant agents for future therapeutic formulations.

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