scholarly journals Exploration and Effectiveness of Trichoderma sp. from Jember and Trenggalek, East Java, Indonesia Cacao Plantation as A Biological Control of Phytophthora palmivora

2021 ◽  
Vol 226 ◽  
pp. 00022
Author(s):  
Henik Sukorini ◽  
Feby Wirasdenty Aigahayunindy ◽  
Erfan Dani Septia ◽  
Netnapis Khewkhom

Fruit rot disease is very damaging to cacao pods, which is caused by Phytoptora palmivora. The attack rate of P. palmivora varies. In Java, losses due to this disease reduce yields by 90 %. P. palmivora is a soil–borne pathogen. It is currently included in the Kingdom Chromista. Control with fungicides is not successful at this time, the alternatives is biologis control with Trichoderma sp. This research used a Completely Randomized Design (CRD) which was arranged in factorial with two factors. The first factor was Trichoderma sp. the second factor was P. palmivora. All treatment combinations were repeated three times. Trichoderma sp. antagonist test to P. palmivora was analyzed using Analysis of Variance (ANOVA) and then further tested using a 5 % BNJ. Trichoderma sp. origin from Jember and Trenggalek districts, East Java, Indonesia were able to act as antagonists against P. palmivora with the highest inhibitory of 78 %. In comparison, the lowest inhibitory was 70 % of isolates from Jember district, East java, Indoe. Characteristics of Trichoderma sp. The origin of Trenggalek Regency and Jember Regency, East Java, Indonesia in inhibiting the growth of P. palmivora has the same species, namely Trichoderma harzianum.

Kultivasi ◽  
2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Lia Amalia ◽  
R Budiasih ◽  
Asep Samsul

Abstract. The decrease in mushroom produc-tivity due to openings at the top of the baglog needs to be balanced with phosphor to increase the mushroom harvest. The research study the interaction effect of opening position plastic baglogs and concentration of phosphor fertilizers to growth and yield of oyster mushroom. The experiment has been done in Mushrooms House, Faculty of Agriculture, Winaya Mukti University, Sumedang with altitude of 850 m above sea level. It was carried out  from  September until December 2015. The experimental design used in this experiment was completely randomized design and treatment design was factorial. The treatment consisted of two factors: the opening position of plastic baglogs and fertilizer concentrations of phosphor fertilizer. Opening position of plastic baglogs consists of a 6 levels that b1 = vertically baglog, the top of baglog opened entirely, b2 = vertically baglog, left side baglog opened at areas 3cm x 1cm, b3 = vertically baglog, left and right side baglog opened at areas 3cm x 1cm, b4 = horizontally baglog, left and right side baglog opened entirely, b5 =  the left side baglog  opened at areas 3cm x 1cm, and b6 = left and right side baglog opened at areas 3cm x 1cm. The concentration of phosphor fertilizer consisted of 3 levels that p0 = 0 g L-1 solution, p1 = 0.25 g L-1 solution, and p2 = 0.50 g L-1 solution. All treatment was replicated 2 times. The results showed that there was interaction between  opening position of plastic baglog and a concentration of phosphor fertilizer to height of mushroom fruiting bodies.Keywords: Baglog, Oyster Mushrooms, Phosphor Sari. Penurunan produktivitas jamur akibat bukaan plastik pada bagian atas baglog perlu diimbangi dengan pemberian fosfor untuk meningkatkan hasil panen jamur tiram. Penelitian bertujuan untuk mempelajari pengaruh interaksi  posisi bukaan plastik baglog dan konsentrasi pupuk fosfor terhadap pertumbuhan dan hasil jamur tiram putih. Percobaan telah dilakukan di Kumbung Jamur Fakultas Pertanian Universitas Winaya Mukti (UNWIM), Kabupaten Sumedang dengan ketinggian tempat 850 m di atas permukaan laut dan dilaksanakan mulai dari bulan  September  sampai bulan Desember 2015. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan rancangan perlakuan adalah faktorial. Perlakuan terdiri dari dua faktor yaitu posisi bukaan plastik  baglog dan konsentrasi pupuk fosfor.  Posisi bukaan plastik baglog  terdiri dari 6 taraf faktor yaitu b1 = baglog diberdirikan, bagian atas baglog dibuka  seluruhnya, b2  = baglog diberdirikan, samping kiri baglog bagian tengahnya dibuka seluas 3cm x 1cm, b3  = baglog diberdirikan, samping kiri dan kanan baglog bagian tengahnya dibuka seluas 3cm x 1cm,  b4  = baglog ditidurkan, samping kiri dan kanan baglog dibuka seluruhnya, b5 = baglog ditidurkan, samping kiri baglog bagian tengahnya dibuka seluas 3cm x 1 cm, dan b6 = baglog ditidurkan, samping kiri dan kanan baglog bagian tengahnya dibuka seluas 3cm x 1 cm  . Konsentrasi pupuk fosfor terdiri dari 3 taraf faktor yaitu p0 = 0 g L-1 Larutan, p1  = 0,25 g L-1 Larutan , dan p2 = 0,50 g L-1 Larutan, sehingga terdiri dari 18 kombinasi perlakuan yang diulang sebanyak 2 kali. Hasil percobaan menunjukkan terjadi interaksi antara posisi bukaan plastik baglog dengan konsentrasi pupuk fosfor terhadap tinggi tubuh buah jamur.Kata kunci : Baglog, Fosfor, Jamur Tiram


2012 ◽  
Vol 13 (3) ◽  
pp. 178
Author(s):  
Sudirman Sudirman ◽  
Karwati Zawani

Once biological control agents of nematodes are introduced into soil, they must function in a very complex and dynamicenvironment. In soil, both nematodes and their antagonists are influenced by other biotic and abiotic factors. However,our knowledge of the effects of these factors in soil is limited. Hence, instead of focusing on the role of individualorganisms in controlling nematode pests, this research aimed to determine the effects of sawdust, microorganisms,and nematodes on trapping activity of Arthrobotrys dactyloides. Experiments were conducted with “standardslide test” and “soil microcosm” using soil amended with various concentrations or without sawdust, with thepresence or the absence of microorganisms, and with the presence or the absence of nematodes (Caenorhabditiselegans and/or Meloidogyne javanica). The experiments were carried out with completely randomized design anddata were analyzed with analysis of variance followed by Tukey’s Honestly Significant Different test. Resultsshowed that sawdust did not have direct effect on ring formation and trapping activity of A. dactyloides. However,sawdust did have effect on microorganisms and C. elegans which then these two factors stimulated ring formationand trapping activity of A. dactyloides.


2020 ◽  
Vol 12 (3) ◽  
pp. 103
Author(s):  
TRI L. MARDININGSIH ◽  
ELNA KARMAWATI ◽  
TRI EKO WAHYONO

ABSTRAK<br />Sanurus indecora merupakan salah satu hama utama yang<br />menyerang tanaman jambu mete di Propinsi Nusa Tenggara Barat. Di<br />Lombok Timur, hama ini diserang oleh cendawan Synnematium sp.<br />Berdasarkan hal tersebut perlu penelitian untuk mengetahui apakah<br />cendawan tersebut dapat digunakan untuk mengendalikan S. indecora.<br />Penelitian yang bertujuan untuk mengetahui peranan Synnematium sp.<br />dalam pengendalian S. indecora dilakukan di Desa Pohgading, Kecamatan<br />Pringgabaya dan di Desa Wanasaba, Kecamatan Wanasaba, Kabupaten<br />Lombok Timur dari bulan Juni sampai September 2004. Penelitian terdiri<br />atas dua kegiatan yaitu di tingkat pot (bibit) dan lapang. Penelitian di<br />tingkat pot terdiri atas tiga kegiatan yaitu aplikasi cendawan terhadap telur,<br />nimfa, imago pada bibit jambu mete (10 telur/ serangga/ bibit). Rancangan<br />yang digunakan ialah acak lengkap dengan empat perlakuan dan diulang<br />enam kali. Pengamatan dilakukan terhadap jumlah telur atau serangga<br />yang mati karena terserang Synnematium sp. mulai satu sampai tujuh hari<br />setelah perlakuan. Penelitian lapang menggunakan rancangan acak<br />kelompok yang disusun secara faktorial dengan dua macam faktor yaitu<br />pola tanam dan konsentrasi Synnematium sp. Parameter yang diamati<br />adalah populasi S. indecora, tingkat serangan, jumlah bunga hermaprodit<br />sebelum perlakuan dan jumlah buah yang berkembang. Hasil penelitian<br />menunjukkan bahwa cendawan Synnematium sp. yang diaplikasikan pada<br />nimfa S. indecora dan bibit menyebabkan kematian serangga tertinggi<br />mencapai 98,33%. Kematian serangga pada ketiga macam konsentrasi<br />Synnematium sp. baik pada telur, nimfa dan imago tidak berbeda nyata.<br />Dalam penelitian ini konsentrasi terkecil yaitu 20 g/l atau setara dengan<br />konsentrasi spora 1,64 x 10 8  sudah efektif menyebabkan kematian S.<br />indecora. Penurunan populasi S. indecora oleh Synnematium sp. efektif<br />dengan konsentrasi 20 g/l sebesar 24,14% dibandingkan dengan kontrol.<br />Tingkat serangan berkorelasi positif dengan populasi serangga.<br />Kata kunci: Jambu mete, Anacardium occidentale L., hama, Sanurus<br />indecora, pengendalian hayati, Synnematium sp. Nusa<br />Tenggara Barat<br />ABSTRACT<br />The role of Synnematium sp. in controling Sanurus<br />indecora JACOBI (Homoptera : Flatidae)<br />Sanurus indecora is one of major pests attacking cashew plants in<br />West Nusa Tenggara Province. In East Lombok, this insect pest was<br />attacked by fungi of Synnematium sp. Based on that, the experiment was<br />conducted to find out whether the fungi could be used to control S.<br />indecora or not. The objective of the experiment was to examine the role<br />of Synnematium sp. in the controlling S. indecora. It was carried out in<br />Pohgading, Pringgabaya and Wanasaba, District of East Lombok from<br />June to September 2004. The experiment consisted of two activities<br />namely polybag stage and field activities. Polybag stage activities<br />consisted of three activities namely application of Synnematium sp. on<br />eggs, nymphs, adults on seedling (10 eggs/insect/seedling). The<br />experiment was arranged in a completely randomized design with four<br />treatments and six replications. Observation was conducted on the number<br />of dead eggs, nymphs and adults attacked by Synnematium sp. from one to<br />seven days after treatment. While field activities used a randomized block<br />design arranged in a factorial with two factors i. e. plant pattern and<br />concentration of Synnematium sp. Parameters observed were population of<br />S. indecora, the degree of attack, the number of hermaphrodite flowers<br />before application and the number of developed fruits. Research results<br />showed that Synnematium sp. sprayed to nymphs of S. indecora and<br />seedling caused the highest mortality of S. indecora that reached 98.33%.<br />Mortality of S. indecora on the three concentrations either on eggs,<br />nymphs and direct application to adults was not significantly different. In<br />this experiment, the smallest concentration i.e. 20 g/l or equivalent with<br />concentration of spore 1.64 x 10 8  was effective to cause the death of S.<br />indecora. The decrease of S. indecora population by Synnematium sp. was<br />effective with concentration of 20g/l as many as 24,14% compared with<br />control. The degree of attack was positively correlated with population of<br />S. indecora.<br />Key words: Cashew, Anacardium occidentale L., pest , Sanurus indecora,<br />biological control, Synnematium sp. Nusa Tenggara Barat


2016 ◽  
Vol 8 (1) ◽  
pp. 24-28
Author(s):  
Maya Indra Rasyid

(Chemical and Organoleptic Characteristics of Mung Bean Flakes with The Addition of Fillers and Water) ABSTRACT. The objective of this research was to determine the chemical and organoleptic characteristics of mung bean flakes with the addition of fillers and water. The study was conducted using completely randomized design with two factors namely the addition of fillers and water. The results showed that the addition of fillers and water has significant (P≤0,01) effect on the water absorption ratio and protein levels of mung bean flakes. The best treatment was obtained by the mung bean flour without the addition of fillers with 150% of addition water with water absorption ratio of 204%, water content of 5,7%, protein of 22,01%, starch levels of 61,76%, organoleptic values of aroma of 2,61, color of 2,42, crispness of 2,72 and flavor of 2,83.


2017 ◽  
Vol 16 (2) ◽  
pp. 79
Author(s):  
Yetti Elfina ◽  
Muhammad Ali ◽  
Rachmad Saputra

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.


ZOOTEC ◽  
2018 ◽  
Vol 39 (1) ◽  
pp. 42
Author(s):  
Agung R. P. Rumondor ◽  
B. Tulung ◽  
A. Rumambi ◽  
C.A Rahasia

EFFECT OF REPLACEMENT OF CORN WITH SORGUM Cv. KAWALI IN PELLET RANSUM ON LOCAL RABBIT PERFORM. The purpose of this study was to determine the effect of substitution corn with sorghum in rabbit rations on feed consumption, body weight gain, and ration conversion. The duration of the study was 28 days using 20 local rabbits. The statistical design used in this study was a completely randomized design, with 4 (four) treatment rations and 5 (five) replications. The four experimental rations, namely R0: 45% corn + 0% sorghum, R1: 30% corn + 15% sorghum, R2 15% corn + 30% sorghum, R3 0% corn + 45% sorghum. Variables were measured, viz: feed consumption, body weight gain and ration conversion. The results showed that the average of feed consumption, body weight gain, and feed conversion, were: 28.27–30.59 g /head /day, 15.76–21.55 g / head /day, and 1.42-1.80 g /head /day, respectively. Based on the analysis of variance, the treatments ration had no significant effect (P> 0.05) on consumption, but had a very significant effect (P <0.01) on body weight gain and feed conversion. The LSD test showed that all treatment rations using sorghum (R1, R2, and R3) gave results of body weight gain which were very significantly different (P <0.01) and higher than R0. Whereas, on ration conversion, treatments R1, R2, and R3 give very different results (P <0.01) lower than R0. It is concluded that the substitution of corn with sorghum at the level of 45% in the ration gives the highest results for body weight gain and conversion of rations for rabbits. Keywords: corn, sorghum, ration, performance, local rabbit


2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.


2021 ◽  
Vol 894 (1) ◽  
pp. 012030
Author(s):  
A T Maryani ◽  
N Mirna ◽  
F F Bahar

Abstract The use of chemical fertilizers in agriculture has caused soil degradation and is harmful to human health. Organic fertilizers made from animal waste have received worldwide attention because they are not detrimental to health or the environment. This paper describes the effect of using organic fertilizers from chicken manure on the growth of papaya plants. A completely randomized design was used in this study using two factors and three repetitions; the variations of the fertilizers given were 0, 50, 100, 150, and 200 g. This study informed that organic chicken manure had a significant effect on the growth of oil palm and papaya plants. The best growth is oil palm and papaya plants given 200 g of organic fertilizer from chicken manure.


2020 ◽  
Author(s):  
Thiwakorn Ampapon ◽  
Bounnaxay Viennasay ◽  
Metha Wanapat

Abstract Background A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate. Results Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation. Conclusion Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.


2016 ◽  
Vol 1 (1) ◽  
pp. 816-823
Author(s):  
Feby Zalika Parindra ◽  
Yusdar Zakaria ◽  
Yurliasni Yurliasni

Abstrak.  Suatu penelitian telah dilakukan untuk mengetahui efek agitasi susu probiotik yang ditambahkan buah naga merah (Hylocereus Polyrhizus). Penelitian ini dilakukan di Laboratorium Ilmu dan Teknologi Pengolahan Susu Fakultas Pertanian Universitas Syiah Kuala pada tanggal 29 April - 31 Mei 2016. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4 dengan 3 kali ulangan yang terdiri dari 2 faktor, A, agitasi yaitu (A1) dan (A2) non agitasi. Dan faktor B yaitu konsentrasi buah naga merah (B1) 20%,  (B2) 25%,  B3 (30%) dan (B4) 35%. Parameter yang diamati adalah derajat keasaman (pH), total mikroba dan uji organoleptik. Hasil penelitian menunujukkan bahwa terdapat pengaruh yang sangat nyata (P0,01) terhadap derajat keasaman (pH) dan berpengaruh nyata terhadap total mikroba (P0,05) serta terdapat interaksi antara kedua perlakuan. Sedangkan untuk uji organoleptik terdapat pengaruh yang sangat nyata (P0,01) terhadap kekentalan dan warna susu probiotik.  nilai derajat keasaman pH 4,03 - 4,11 dengan agitasi (pengadukan) masih sesuai dengan standar (SNI) 01-2981-1992. Sedangkan nilai total mikroba optimal terjadi pada penambahan buah naga sebanyak 30% baik agitasi maupun non agitasi. Effects of Milk Probiotic Agitation Added Red Dragon Fruit (Hylocereus Polyrhizus) on Sensory Test and Total Plate Coun Abstract.  A study about effects of milk probiotic agitation added red dragon fruit (Hylocereus Polyrhizus)  has been conducted to determine to test sensory and total plate count. This research was held at the Laboratory of Science and Milk Processing Technology, Agriculture Fakulty Syiah Kuala University on April 29 to May 31, 2016. This study used a completely randomized design (CRD) 2x4 factorial design with three replications consisting of two factors, A, agitation (A1) and (A2) non agitation. And factor B is the concentration of red dragon fruit (B1) 20%, (B2) 25%, B3 (30%) and (B4) 35%. The observed parameter is the degree of acidity (pH), total microbial and organoleptic tests. The results of the study indicate that the there is a  highly significant influence (P 0,01) to the degree of acidity (pH) and total significantly affected the total microbial (P 0.05), and there is interaction between the two treatments. As for organoleptic tests are highly significant effect (P 0.01) the thickness and color of probiotic milk. acidity pH value of 4.03 to 4.11 with agitation (stirring) is still in accordance with the standard (SNI) 01-2981-1992. While the total value of optimal microbial occurred upon addition of dragon fruit by 30% both agitation and non agitation


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