scholarly journals PENGARUH EKSTRAK GULMA SIAM, KEMUNING DAN SALIARA TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI LAYU PISANG SECARA IN VITRO

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Nova Adelina Lubis ◽  
Titik Nur Aeny ◽  
Joko Prasetyo ◽  
Radix Suharjo

Salah satu penyakit penting pada tanaman pisang adalah penyakit layu bakteri, yang disebabkan olehBlood Disease Bacterium (BDB). Beberapa jenis tanaman telah dilaporkan mengandung senyawa antibakteri yang dapat menjadi alternatif pengendalian penyakit tanaman yang disebabkan oleh bakteri. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak Chromolaena odorata, Murraya paniculata dan Lantana camara terhadap penghambatan perrtumbuhan BDB secara in vitro. Penelitian dilakukan di Laboratorium Bioteknologi Fakultas Pertanian Universitas Lampung pada Maret hingga Juli 2016. Perlakuan disusun menggunakan Rancangan Acak Lengkap dalam faktorial. Faktor pertama adalah lama perendaman bahan tanaman sebelum diekstrak dan faktor kedua adalah tingkat konsentrasi ekstrak. Pengamatan dilakukan terhadap diameter zona penghambatan pada 24 jam setelah aplikasi. Data diuji dengan analisis ragam dan nilai tengah antar perlakuan diuji dengan uji Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan bahwa pada perlakuan ekstrak kemuning, interaksi antara lama perendaman dan tingkat konsentrasi berpengaruh nyata terhadap diameter zona penghambatan BDB sedangkan pada ekstrak gulma siam dan saliara interaksinya tidak nyata. Diameter zona penghambatan paling tinggi ekstrak gulma siam, kemuning dan saliara dalam menghambat pertumbuhan BDB secara berurutan adalah 85%, 55% dan 95%.

2020 ◽  
Vol 3 (4) ◽  
pp. 244-251
Author(s):  
Hung Thanh Tran ◽  
My-Ngan Thi Luong ◽  
Le Van Bui ◽  
Hieu Trung Tran

The current study was carried out to evaluate antifeedant and insecticidal activities of three essential oils from leaves of Hyptis suaveolens, Chromolaena odorata and Lantana camara against fourth instar larvae of Spodoptera litura under the laboratory conditions. Results indicated that the essential oil from leaves of Hyptis suaveolens had a strong antifeedant impact on the larvae with antifeedant index (AI) of 75.4, 88.5 and 92.9 when leaf disks of Ipomoea batatas were treated with the essential oil at concentration of 1.5, 2.0 and 2.5 %, respectively. Also dose dependent mortalities were observed in the larvae topical treated with the essential oil at different concentrations. At the treated concentration of 0.4 mg per each larva, 68.3 % of the larvae were killed after 24 hours, and 25.0 % were died at the pupal stage after 30 days of observation. At the concentration of 1.2 mg/larva, 93.3 % of the larvae were killed after 24 hours, and no emergence of adults was recorded after 30 days of observation. Larvicidal activity of the H. suaveolens essential oil (with the lethal doses LD50=0.16 and LD95=1.52 mg/larva) was stronger than those of the C. odorata and L. camara essential oils (with LD50=0.57–0.63 and LD95=4.64–4.97 mg/larva) after 24 hour of treatment. GC–MS analysis indicates that the major volatile components of the essential oil from leaves of H. suaveolens are composed of b-caryophyllene (30.0 %), eucalyptol (12.0 %), copaene (5.9 %), and a-bergamotene (5.7 %). This essential oil needs to be further investigated and used as a bio-insecticide for control of S. litura.  


Author(s):  
Nishita Gogia ◽  
Anandhi D ◽  
Kanaga G ◽  
Revathi K

The scope of the present study was to evaluate the In-vitro biological potential of Green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf). We investigated the effect of green synthesized silver nanoparticles from Chromolaena odorata, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) by studying anti-mitotic activity An attempt has been made to evaluate the anti-mitotic activity of silver nanoparticles using Allium cepa roots. The mitotic index of the root tips meristem was calculated and compared with the standard methotrexate. Antimitotic activity results shows the mitotic index was ranged from 30-40%. Green synthesized silver nanoparticles from Chromolaena odorato, Caesalpinia coriaria (Bark) and Caesalpinia coriaria (Leaf) possess significant anti-mitotic activity.


2020 ◽  
Vol 101 ◽  
pp. 104004 ◽  
Author(s):  
Peng Wu ◽  
Ziteng Song ◽  
Xuelian Wang ◽  
Ying Li ◽  
Yuhao Li ◽  
...  

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 393-394
Author(s):  
Adebayo O Oni ◽  
Bobby-Joe Ogadu ◽  
Azeez O Yusuf ◽  
A Adebowale ◽  
Oluwakemi Oni ◽  
...  

Abstract Recently, bioactive component of plant and plant parts have been used as rumen modifier to reduce methane gas production in ruminant livestock so as to reduce their contribution to the implicated greenhouse effect. This study therefore, evaluated the potential of Siam weed (Chromolaena odorata) leaves as a modifier of rumen fermentation in West African dwarf bucks. Fresh C. odorata leaves were harvested, air dried (3 weeks), milled using a 2mm sieve size and bagged for both proximate and phytochemical analysis. Concentrate diets were formulated with C. odorata leaf meal included in the diet at 0, 2, 4 and 6% of the whole diet. Rumen fluids were collected from West African dwarf (WAD) bucks (averaged 25kg) using suction tube and randomly allotted to the 4 experimental diets in a Completely Randomized Design. Incubation of inoculums was done for 96hrs with 12 replicates per treatment in a single run. Data obtained were analyzed using a One-way Analysis of Variance and means compared using Tukey’s Test. Results indicated that C. odorata had 969.0mg/kg dry matter, 175.1mg/kg crude protein, 204.3mg/kg crude fibre, 521.6mg/kg nitrogen free extract, 19.9 mg/kg saponin, 25.7 mg/kg tannin, 10.8mg/kg flavonoid and 12.6 mg/kg alkaloid. The addition of 2 and 4% C. odorata to the diets resulted in increased (P < 0.05) in vitro gas production while C. odorata at 2 % reduced (P < 0.05) the methane gas (%) estimate. In vitro organic and dry matter digestibilities, total digestible substrates and short chain fatty acids were increased (P < 0.05) with C. odorata addition to the diets. This study concluded that the use of C. odorata as an additive at 2 and 4% inclusion increased total gas output; however, 2% inclusion will be beneficial as it reduced the methane output while maintaining higher gas production and digestibility.


2007 ◽  
Vol 18 (8) ◽  
pp. 1504-1508 ◽  
Author(s):  
Vanessa Ribeiro Affonso ◽  
Humberto Ribeiro Bizzo ◽  
Sharon Santos de Lima ◽  
Maria Apparecida Esquibel ◽  
Alice Sato

2012 ◽  
Vol 67 ◽  
pp. 0381 ◽  
Author(s):  
Lamia T. Abou El-Kassem ◽  
Reda S. Mohammed ◽  
Sahar S. El Souda ◽  
Amira A. El-Anssary ◽  
Usama W. Hawas ◽  
...  
Keyword(s):  

Nematology ◽  
2007 ◽  
Vol 9 (3) ◽  
pp. 343-349 ◽  
Author(s):  
Michael Boppré ◽  
Tim Thoden ◽  
Johannes Hallmann

Abstract1,2-dehydropyrrolizidine alkaloids (PAs) represent a class of secondary plant compounds that are active in defence against herbivory. They are present in Chromolaena odorata, one of the most invasive weeds of Asia and Africa. In vitro studies demonstrate that pure PAs from C. odorata roots have nematicidal effects on the root-knot nematode Meloidogyne incognita, even at concentrations of 70-350 ppm. In vivo experiments show that mulch or aqueous crude extracts from C. odorata roots reduce the infection of lettuce by M. incognita. Thus, the use of PA-containing plants appears to be a valuable element for integrated nematode management.


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