scholarly journals Genetic Variation of Sinorhizobium meliloti Isolates Differing in Their Ability to Drought Tolerance

2015 ◽  
Vol 9 (2) ◽  
pp. 7-22
Author(s):  
Rana A. Hameed Hameed ◽  
Nidhal N. Hussain Hussain ◽  
Abed aljasim M. Aljibouri Aljibouri

Soil bacteria Sinorhizobium meliloti had enormous agricultural value, due to their ability infixing nitrogen symbiotically with an important forage crop legume- alfalfa. The aim of thisstudy (i) isolate indigenous S. meliloti from different field sites in Iraq, (ii) evaluate the isolatestolerance to induce drought using polyethylene glycol-6000, (iii) assessing genetic diversity andgenetic relationships among isolates of natural population with drought tolerant abilities.Drought tolerance study revealed vast variations between Sinorhizobium isolates, the highesttolerant isolates to drought were twelve from total thirty 40%, tolerated from -3 up to -4 Mpa(mega pascal), while the drought sensitive isolates tolerated upto – 1.5 Mpa, except isolate Bs58which tolerated upto -1 Mpa water potential. The growth declined with the increase of droughtstress. Cluster analysis based on RAPD-PCR showed significant differences among S. melilotiisolates, and the results gave almost identical grouping of isolates in regards to droughtexperiment. Among indigenous isolates two divergent groups could be determined, the first majorgroup included drought tolerant isolates and the second major group comprised all droughtmoderate and sensitive isolates with 40% similarity between the two major groups.

2016 ◽  
Vol 6 (1) ◽  
pp. 20 ◽  
Author(s):  
Rana Azeez Hameed ◽  
Nidhal Neema Hussain ◽  
Abd aljasim Muhisen Aljibouri

<p class="1Body"><em>Sinorhizobium meliloti </em>is a gram-negative, soil bacteria, which gain a huge importance deserved to their capability in fixing nitrogen symbiotically with an important fodder crop legume-alfalfa (<em>Medicago sativa</em>). This study aims to (i): isolate indigenous <em>Sinorhizobium meliloti</em> from different field sites in Iraq; (ii): assess the isolates tolerance to induced water shortage using polyethylene glycol-6000; (iii): evaluate genetic diversity and genetic relationships among isolates of natural population with drought tolerant abilities. Drought tolerance analysis disclose great variations between <em>Sinorhizobium</em> isolates, the highest tolerant isolates to drought were 12 from whole thirty (40%), endured from -3 up to -4 MPa(Mega pascal), at the same time the drought sensitive isolates tolerated up to -1.5 MPa, but for isolate Bs58 which tolerated up to -1 MPa. The growth decrease with the increase of drought stress. REP-PCR method was an excellent technique to distinguish between the <em>S. </em>isolates based on their tolerance to different water potential levels, the REP-PCR gave a significant band about 700bp (base pair) in size, which were present in all drought moderate and tolerant isolates, while this band was absent in all drought sensitive isolates(except one isolate). Also the ERIC-PCR method cleared the absence of two significant bands 300bp and 600bp from all drought sensitive isolates. UPGMA analysis based on rep-PCR revealed two groups branching at a similarity of 35%, first group included only one isolate Bs16, which was a drought moderate tolerant; second group contained the rest of isolates, yet the latter splits into two subgroups with 68% similarity; the first subgroup comprised all sensitive isolates with 80% similarity among them, the second subgroup included the tolerant and moderate isolates with similarity 88%.</p>


2019 ◽  
Vol 144 (6) ◽  
pp. 379-386
Author(s):  
Yan Liu ◽  
Hailin Guo ◽  
Yi Wang ◽  
Jingang Shi ◽  
Dandan Li ◽  
...  

Seashore paspalum (Paspalum vaginatum) is a notable warm-season turfgrass. Certain germplasm resources are distributed in the southern regions of China. The objectives of this study were to investigate the genetic diversity and genetic variation of Chinese seashore paspalum resources. Morphological characteristics and sequence-related amplified polymorphism (SRAP) markers were used to assess genetic relationships and genetic variation among 36 germplasm resources from China and six cultivars from the United States. The results showed significant variation for 13 morphological characteristics among 42 tested seashore paspalum accessions, and that the phenotypic cv was, in turn, turf height > turf density > internode length > inflorescence density > leaf width > reproductive branch height > spikelet width > leaf length > spikelet number > inflorescence length > internode diameter > inflorescence width > spikelet length. According to the morphological characteristics and cluster analysis, 42 seashore paspalum accessions were divided into six morphological types. In total, 374 clear bands were amplified using 30 SRAP primer combinations; among these bands, 321 were polymorphic with 85.83% polymorphism. SRAP marker cluster analysis showed that 42 seashore paspalum accessions were grouped into seven major groups, with a genetic similarity coefficient ranging from 0.4385 to 0.9893 and genetic distance values ranging from 0.0108 to 0.8244. The high level of genetic diversity occurred among Chinese germplasm, and the genetic distance was relatively high between Chinese germplasm and cultivars introduced from the United States. The patterns in morphological trait variations and genetic diversity will be useful for the further exploitation and use of Chinese seashore paspalum resources.


2018 ◽  
Vol 28 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Abbas Saidi ◽  
Zahra Daneshvar ◽  
Zohreh Hajibarat

To evaluate the genetic diversity among 10 cultivars of anthurium were performed using three molecular markers such as Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP), and Random Amplification of Polymorphic DNA (RAPD). Polymorphism index content (PIC) was calculated 0.39, 0.42 and 0.37 for RAPD, SCoT and CDDP, respectively. This result showed all the three molecular markers had almost an identical potential in estimating genetic diversity. Cluster analysis using SCoT, CDDP and RAPD divided the cultivars to three distinct clusters. The similarity matrix obtained through SCoT and CDDP was positively significantly correlated (r = 0.76, p < 0.01). This is the first report in which the efficiency of two targeted DNA region molecular markers (SCoT and CDDP) together with RAPD technique have been compared with each other in a set of anthurium cultivras. Results suggested that SCOT, CDDP and RAPD fingerprinting techniques are of sufficient ability to detect polymorphism in anthurium cultivars. Plant Tissue Cult. & Biotech. 28(2): 171-182, 2018 (December)


2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Rahayu Rahayu ◽  
Fatimah Suwardjo ◽  
Ji Bae Eun ◽  
Geun Mo Yang ◽  
Soo Choi Joon

Abstract. Rahayu, Fatimah, Bae EJ, Mo YG, Choi JS. 2020. Genetic diversity and morphological characteristics of native seashore paspalum in Indonesia. Biodiversitas 21: 4981-4989. Seashore paspalum (Paspalum vaginatum) is a warm-season turfgrass indigenous to tropical and coastal areas worldwide. The objectives of this study were to measure the genetic diversity and genetic variation of Indonesian seashore paspalum germplasm. Three turf quality, six morphological characters, and ten SSR (microsatellite) markers were used to assess genetic relationships and genetic variation among 22 germplasm resources from Indonesia and one commercial variety (Salam) from United States of America. The results showed significant variation for five morphological characters among 23 tested seashore paspalum accessions. The cluster analysis of morphological characters of 23 seashore paspalum accessions using 0,6 cut off divided into three morphological types: tall high-density, intermediate, and dwarf low-density ecotype. The genetic variation revealed 22 alleles with average number of alleles per locus was 2 and polymorphism information content (PIC) values average was 0.33. The microsatellite marker cluster analysis showed that 23 seashore paspalum accessions were grouped into two major groups, with a genetic similarity coefficient was 0,72. The low level of genetic diversity occurred among Indonesia natural grass germplasm and the genetic distance was relatively low between Indonesian germplasm and Salam variety. The genetic diversity and morphological characteristics will be useful for further study and utilization of Indonesian seashore paspalum germplasm.


Author(s):  
May Sandar Kyaing ◽  
Sein Sandar May Phyo

This study was conducted to explore the genetic diversity and relationship of Sein Ta Lone mango cultivars among 20 commercial orchards in Sintgaing Township, Mandalay region. Nine microsatellite (SSR) markers were used to detect genetic polymorphism in a range from (3 to 6) alleles with (4.33) alleles per marker in average. Six out of nine microsatellite markers gave the PIC values of greater than (0.5). Among them, SSR36 held the highest PIC values of (0.691) while MiSHRS39 and MN85 possessed the least PIC values of (0.368) and (0.387) respectively. The genetic diversity was expressed as unbiased expected heterozygosity (UHe) value with an average of (0.561). The genetic relationship was revealed by (UPGMA) dendrogram in a range of (0.69 to 1.00). Based on UPGMA cluster analysis, three main clusters were classified among three different locations. This study was intended to help cultivar characterization and conservation for proper germplasm management with the estimation of genetic variation and relationship in the existing population of Sein Ta Lone mangoes in Sintgaing Township by microsatellite markers.  


2011 ◽  
Vol 18 (2) ◽  
pp. 93-104 ◽  
Author(s):  
Fatemeh Mohammadzadeh ◽  
Hassan Monirifar ◽  
Jalal Saba ◽  
Mostafa Valizadeh ◽  
Ahmad Razban Haghighi ◽  
...  

Genetic diversity among and within 10 populations of Iranian alfalfa, from different areas of Azarbaijan, Iran was analyzed by screening DNA from seeds of individual plants and bulk samples. In individual study, 10 randomly amplified polymorphic DNA (RAPD) primers produced 156 polymorphic bands and a high level of genetic diversity was observed within populations. The averages of total and within population genetic diversity were 0.2349 and 0.1892, respectively. Results of analysis of molecular variance (AMOVA) showed the great genetic variation existed within populations (81.37%). These Results were in agreement with allogamous and polyploid nature of alfalfa. Cluster analysis was performed based on Nei’s genetic distances resulting in grouping into 3 clusters which could separate breeding population from other populations. Results of cluster analysis were in consistent with morphological and geographical patterns of populations. The results of bulk method were different from individual analysis. Our results showed that RAPD analysis is a suitable method to study genetic diversity and relationships among alfalfa populations.Keywords: Alfalfa; RAPD; Genetic diversity; Analysis of Molecular Variance; Cluster analysis.DOI: http://dx.doi.org/10.3329/bjpt.v18i2.9296Bangladesh J. Plant Taxon. 18: (2): 93-104, 2011 (December)


2013 ◽  
Vol 13 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Claudia Teresa Hornung-Leoni ◽  
Victoria Sosa ◽  
June Simpson ◽  
Katia Gil

Puya raimondii, the giant Peruvian and Bolivian terrestrial bromeliad, is an emblematic endemic Andean species well represented in Huascarán National Park in Peru. This park is the largest reserve of puna (high altitude plateau) vegetation. The objective of this study is to report on genetic variation in populations of P. raimondii from Huascarán and neighboring areas. AFLP profiles with four selective primer combinations were retrieved for 60 individuals from different zones. Genetic variability was estimated and a total of 172 bands were detected, of which 79.1% were polymorphic loci. The results showed genetic differentiation among populations, and gene flow. A cluster analysis showed that individuals of P. raimondii populations located in different mountain systems could be grouped together, suggesting long distance dispersal. Thus, conservation strategies for P. raimondii have to take into account exchange between populations located far apart in distance in order to preserve the genetic diversity of this showy species.


2011 ◽  
Vol 49 (No. 3) ◽  
pp. 106-113 ◽  
Author(s):  
Shengwu Hu ◽  
J. Ovesná ◽  
L. Kučera ◽  
V. Kučera ◽  
M. Vyvadilová

The genetic diversity and the relationships among rapeseed germplasm, including a&nbsp;collection of 20 Chinese, 25 Czech, 2&nbsp;German, 2 French, and 1 English cultivars and breeding materials were evaluated using random amplified polymorphic DNA (RAPD) markers. A&nbsp;total of 79 different polymorphic amplification products were obtained using10 selected decamer primers. RAPDs revealed a&nbsp;significant level of polymorphism among the accessions. The diversity index (DI) ranged from 1.390 to 3.491, showing a&nbsp;sufficient potential of selected primers to differentiate among studied genotypes. Three different metrics were used to assess genetic diversity. The best fit between a&nbsp;priori knowledge about germplasm origin and a&nbsp;posteriori grouping was found using Hamman metrics. Cluster analysis based on Hamman pairwise distance comparison divided the studied accessions into three main clusters. The first group included only accessions fromChina, the second group only that fromEurope with the exception of Zhongshuang No. 2, a&nbsp;Chinese winter rape possessing European cultivars in the pedigree. The third group included accessions both fromChina andEurope. The results indicate the occurrence of a&nbsp;considerable genetic variation between Chinese and European accessions.


2021 ◽  
Vol 910 (1) ◽  
pp. 012050
Author(s):  
Mohammed Ibrahim Mohammed ◽  
Khalid Mohammed Al-Zubaidy ◽  
Balssam Mansour Al Obaidi

Abstract The study included twenty genotypes of triticale, whose seeds were sown during 2018-2019 season at the Research Station of the Faculty of Agriculture, University of Kirkuk in the Sayyadah region on three dates (5 November, 20 November and 5 December) using randomized complete block design according to split plot system with three replications. The data were recorded for traits: first, second and third developmental stages, number of days to 50% flowering, plant height, flag leaf area, number of tillers per plant, number of spikes per plant, length and weight of spike, number of spikelet’s per spike, number of grains per spike, 1000 grains weight, biological yield, grain yield per plant, harvest index, protein percent, specific weight, gluten percent, flour strength, moisture percent and ash percentage, The data were analyzed to identify the nature of the differences between genotypes and planting dates. Because of the significant (genotypes x planting dates) interaction, a cluster analysis was conducted with the aim of grouping similar genotypes into homogeneous groups and estimating the degree of genetic diversity between them through the use of hierarchical clustering technology to estimate distances between groups of genotypes formed for each planting date separately. The results showed that the mean squares of genotypes' was highly significant 1% for all traits except harvest index, with a highly significant interaction with dates for all traits except number of spikelet’s and protein percent. The stages of the cluster analysis showed that the genotypes were distributed into 13 groups for the first date and 14 groups for the second and third dates. Some groups included one genotype, indicating the difference of these genotypes from other due to the difference in their genetic origin, which was consequently reflected on their performance, while other groups includes two genotypes. It is concluded from the results of the clustering analysis that there is a strong convergence between the genotypes of stage 18 with the genotype LIRON at the first date and with POLLMER in the second and third dates because they have the lowest euclidean distances, and this requires avoiding crosses between these pairs, while the highest distance was between CMH80 and CMH82 in the first and third dates and CENT/1715 and POPP-CAAL in the second date indicated high genetic variation between them and other genotypes, which may be due to the variation in their genetic origin or to having preferred main genes, other genotypes devoid of them, which encourages their introduction into hybridization with genotypes that showed distinct genetic variation to take advantage of the phenomenon of heterosis and its segregations.


2016 ◽  
Vol 58 ◽  
pp. 42-53 ◽  
Author(s):  
Pham Thi Thu Ha ◽  
Do Tan Khang ◽  
Phung Thi Tuyen ◽  
Luong The Minh ◽  
Truong Ngoc Minh ◽  
...  

In this study, the correlation coefficients among agro-morphological variation, genetic diversity, and drought tolerance in 44 rice cultivars were analyzed. The drought tolerance at seeding stage (DTS) was significantly proportional to drought tolerance at vegetative stage (DTV) (r = 0.60). In addition, DTS and DTV had strong significant positive correlation to leaf roll (r = 0.87 and 0.54, respectively). Means of unfilled grains and tilling per panicle were proportionally correlated to DTS (r = 0.22 and 0.25, respectively), and DTV (r = 0.20 and 0.36, respectively). However, weight of 1000 grains and filled grains recorded no correlation to DTS and DTV. At a homologous coefficient of 16.85 integrated from cluster analysis of agro-morphological, quantitative characteristics and drought tolerant scores, the rice was divided into five groups. Maximum PIC (polymorphic information content) values were detected in three markers including RM11125, RM21, and RM5629, which were between 0.78-0.79. Cluster analysis of microsatellite markers revealed that by a genetic distance of 0.63, the rice varieties were separated into three clusters. The results provide valuable information for rice breeders to select donors in breeding rice integrated with drought tolerance and good agronomic characteristics.


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