scholarly journals Stability Studies of Immobilized Saccharomyces Cerevisiae in Calcium Alginate and Carrageenan Beads

2017 ◽  
Vol 2 (4) ◽  
pp. 10 ◽  
Author(s):  
Rahmath Abdulla ◽  
Warda Abdul Ajak ◽  
Siti Hajar ◽  
Eryati Derman
Keyword(s):  
Calcium Alginate ◽  
Alternative Fuels ◽  
Fossil Fuels ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Bioethanol Production ◽  
Stability Studies ◽  
Fuel Prices ◽  
The Stability ◽  
Liquid Biofuel

Currently the resources for fossil fuels are depleting together with increase in fuel prices. This has urged the need for cheaper alternative fuels especially biofuels. The production of the most common liquid biofuel which is bioethanol using immobilized yeast cells is an approach taken to increase its demand in the world’s market. There are various methods for the immobilization of yeast cells; however before they can be applied in the industry the stability of the immobilization technology must be investigated. This research aims to study the stabilities of immobilized S. cerevisiae in calcium alginate and carrageenan beads for bioethanol production. The S. cerevisiae was immobilized in calcium alginate and carrageenan beads using entrapment method. Next, screening for the optimal concentration of sodium alginate and semi refined carrageenan matrices were determined by employing fermentation and bioethanol quantification using GC-MS. Concentrations of 2% (w/v) calcium alginate and 2% (w/v) semi refined carrageenan beads were identified to produce the highest bioethanol yield which were 0.286 g/mL and 0.065 g/mL respectively. The two beads were then chosen to be tested in various stability studies with respect to bioethanol production such as storage stability, reusability, pH, thermaland permeability test. It was found out that a concentration of 2% (w/v) calcium alginate beads were more stable as immobilization matrix for S. cerevisiae  as compared to 2% (w/v) semi refined carrageenan.

scholarly journals Improving the Stability of Astaxanthin by Microencapsulation in Calcium Alginate Beads

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153685 ◽  
Author(s):  
Shen-Fu Lin ◽  
Ying-Chen Chen ◽  
Ray-Neng Chen ◽  
Ling-Chun Chen ◽  
Hsiu-O Ho ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
The Stability

scholarly journals Optimization of alcoholic fermentation using immobilized yeast cells in calcium alginate gel

2015 ◽  
pp. 207-218
Author(s):  
Jovana Djuran ◽  
Zorana Roncevic ◽  
Bojana Bajic ◽  
Sinisa Dodic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Yeast Extract ◽  
Fossil Fuels ◽  
Alcoholic Fermentation ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Glucose Content ◽  
Alginate Concentration ◽  
Economic Analyses

Ethanol is an important industrial chemical with emerging potential as a biofuel to replace fossil fuels. In order to enhance the efficiency and yield of alcoholic fermentation, combined techniques such as cells immobilization and media optimization have been used. The aim of this study was the optimization of sodium alginate concentration and glucose and yeast extract content in the media for ethanol production with immobilized cells of Saccharomyces cerevisiae. Optimization of these parameters was attempted by using a Box-Behnken design using the response surface methodology. The obtained model predicts that the maximum ethanol content of 7.21% (v/v) is produced when the optimal values of sodium alginate concentration and initial content of glucose and yeast extract in the medium are 22.84 g/L, 196.42 g/L and 3.77 g/L, respectively. To minimize the number of yeast cells "eluted" from the alginate beads and residual glucose content in fermented media, additional two sets of optimization were made. The obtained results can be used for further techno-economic analyses of the process to select the optimum conditions of the fermentation process for industrial application.

Adsorption of ochratoxin A from grape juice by yeast cells immobilised in calcium alginate beads

2016 ◽  
Vol 217 ◽  
pp. 29-34 ◽  
Author(s):  
Maria Grazia Farbo ◽  
Pietro Paolo Urgeghe ◽  
Stefano Fiori ◽  
Salvatore Marceddu ◽  
Samir Jaoua ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Calcium Alginate ◽  
Grape Juice ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Calcium Alginate Beads

scholarly journals INMOVILIZACIÓN DE LAS MICROALGAS Scenedesmus ovalternus (Scenedesmaceae) Y Chlorella vulgaris (Chlorellaceae) EN ESFERAS DE ALGINATO DE CALCIO

2016 ◽  
Vol 21 (2) ◽  
pp. 437-442
Author(s):  
Mario Andres Forero-Cujiño ◽  
Luis Carlos Montengro Ruiz ◽  
Gabriel Antonio Pinilla-Agudelo ◽  
Luz Marina Melgarejo-Muñoz
Keyword(s):  
Growth Rate ◽  
Population Density ◽  
Chlorella Vulgaris ◽  
Calcium Alginate ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
The Stability ◽  
Kinetics Of

<p>En este trabajo se describe la técnica de inmovilización de microalgas en esferas de alginato de calcio. Se emplearon las especies Scenedesmus ovalternus y Chlorella vulgaris, se determinó la estabilidad de las esferas, la cinética de crecimiento y la concentración de las microalgas en el interior de las esferas. Chlorella vulgaris alcanzó mayores densidades poblacionales y tasas de crecimiento más altas cuando se inmovilizó en concentraciones del 10 % v/v con el alginato (1,31*10<span>6 </span>cél/ml). Para Scenedesmus ovalternus se observó una mayor densidad poblacional y una mayor tasa de crecimiento cuando se inmovilizó en concentraciones del 20 % v/v (7,06*10<span>5 </span>cél/ml). Estos resultados son útiles para aplicaciones prácticas de las algas encapsuladas, tales como el biomonitoreo o la biorremediación.</p><p><strong>Immobilization of Microalgae<em> Scenedesmus ovalternus</em> (Scenedesmaceae) and <em>Chlorella vulgaris</em> (Chlorellaceae) in Calcium Alginate Beads</strong></p><p>This paper describes the immobilization technique of microalgae in calcium alginate beads. Scenedesmus ovalternus and Chlorella vulgaris species were used. The stability of beads, the kinetics of growth and the concentrations of microalgae inside the beads were determined. The higher density and the upper growth rate of Chlorella vulgaris occurred when it was immobilized in alginate at a concentration of 10 %v/v (1,31*10<span>6 </span>cél/ml). Scenedesmus ovalternus achieved a higher population density and an elevated growth rate when it was immobilized at a concentration of 20 % v/v (7,06*10<span>5 </span>cél/ml). These results are useful for subsequent applications of the encapsulated algae, such as biomonitoring and bioremediation.</p>

scholarly journals Experimental Investigation on Performance, Combustion, Emission and Vibration Analysis of Diesel Engine Fuelled with Rice Bran Biodiesel and n-Butanol Additive

2021 ◽  
Author(s):  
Rajendra Pawar ◽  
◽  
Sharad Patil ◽  
Kamalesh Jagadale ◽  
Pranali Gujar ◽  
...  
Keyword(s):  
Diesel Engine ◽  
Rice Bran ◽  
Diesel Fuel ◽  
Alternative Fuels ◽  
Fossil Fuels ◽  
Nitric Oxides ◽  
Promising Alternative ◽  
Fuel Prices ◽  
Engine Cylinder ◽  
Combustion Emission

Substantial growth in emissions, hike in fuel prices, and exhaustion of fossil fuels has given rise to the need for substitute fuels for diesel engines, which are renewable and demote the emission. Also, strict international emission standards force researchers to seek alternative fuels. Vegetable oils are promising alternative biodiesel for a diesel engine, amongst them, rice bran is underutilized, a non-edible source that doesn’t create any food security hurdle. The paper focused to investigate the performance, combustion, emission, and vibration characteristics of diesel engine fuelled with rice bran biodiesel and n- butanol additive (5% constant) at CR 17.5. The engine characteristics of seven biodiesel blends (B5n5, B10n5, B15n5, B20n5, B25n5, B30n5, and B40n5) were measured at various loads under constant speed and compared with diesel fuel. The performance characteristics were observed in moderate quantities as compared to diesel whereas the emissions were found reduced drastically than diesel fuel except for nitric oxides (NOx) emissions. The measured engine cylinder vibration for all blends indicates similar results as diesel fuel hence leads to smooth combustion. The investigation shows that blends from B20n5 to B30n5 have the potential to be used in a diesel engine without any modification.

A novel method for bioethanol production using immobilized yeast cells in calcium-alginate films and hybrid composite pervaporation membrane

2018 ◽  
Vol 247 ◽  
pp. 165-173 ◽  
Author(s):  
Eduardo Leal Isla Santos ◽  
Magdalena Rostro-Alanís ◽  
Roberto Parra-Saldívar ◽  
Alejandro J. Alvarez
Keyword(s):  
Hybrid Composite ◽  
Calcium Alginate ◽  
Yeast Cells ◽  
Bioethanol Production ◽  
Immobilized Yeast ◽  
Pervaporation Membrane ◽  
Immobilized Yeast Cells ◽  
Novel Method

A perspective on bioethanol production from biomass as alternative fuel for spark ignition engine

RSC Advances ◽  
2016 ◽  
Vol 6 (18) ◽  
pp. 14964-14992 ◽  
Author(s):  
A. H. Sebayang ◽  
H. H. Masjuki ◽  
Hwai Chyuan Ong ◽  
S. Dharma ◽  
A. S. Silitonga ◽  
...  
Keyword(s):  
Fuel Consumption ◽  
Alternative Fuels ◽  
Fossil Fuels ◽  
Alternative Fuel ◽  
Spark Ignition ◽  
Spark Ignition Engine ◽  
Bioethanol Production ◽  
The Future

The increasing fuel consumption of fossil fuels has led to the development of alternative fuels for the future.

scholarly journals Sparkling wine production by immobilised yeast fermentation

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 171-179 ◽  
Author(s):  
Miličević Borislav ◽  
Babić Jurislav ◽  
Ačkar Đurđica ◽  
Miličević Radoslav ◽  
Jozinović Antun ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Alginate Beads ◽  
Aroma Compounds ◽  
Yeast Cells ◽  
Yeast Fermentation ◽  
Sparkling Wine ◽  
Alcohol Content ◽  
Wine Production ◽  
Grape Varieties ◽  
Immobilised Yeast

The prospects of sparkling wine production by the ‘Champenoise’ method using alginate-immobilised yeast cells were examined. Grape varieties dominant in quantity were selected within the group of recommended and permitted varieties of Kutjevo vineyards, located in the eastern part of continental Croatia. Research revealed that there are no influential variations in the principal physicochemical and sensory characteristics between sparkling wines obtained through immobilised yeast and traditional sparkling method. The analysis of aroma compounds showed minor differences between samples. Observed oenological parameters assessed in the final products did not show any relevant oenological differences, with the exception of alcohol content, which was slightly higher in sparkling wines made with yeast cells immobilised with calcium alginate beads. According to this research, the sensory properties of the produced sparkling wines, compared to sparkling wine produced with free yeast, did not show any significant differences. On the full-scale obtained results indicate that some of the selected varieties can be sorted as suitable for the production of sparkling wine using immobilised yeast cells.

EFFECT OF CALCIUM ALGINATE COATING ON THE PERFORMANCE OF IMMOBILIZED YEAST CELLS IN CALCIUM ALGINATE BEADS

2000 ◽  
Vol 177 (1) ◽  
pp. 1-14 ◽  
Author(s):  
YOSHIMITSU UEMURA ◽  
NAOKl HAMAKAWA ◽  
HIDEKAZU YOSHIZAWA ◽  
HIROKI ANDO ◽  
KAZUYA IJICHIY ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Immobilized Yeast ◽  
Calcium Alginate Beads ◽  
Immobilized Yeast Cells ◽  
Alginate Coating

scholarly journals A Study on Technical and PM Emission Characteristics on Diesel Engines using Biodiesel Based Palm Oil

2020 ◽  
Vol 5 (2) ◽  
pp. 151-154
Author(s):  
Van Viet Pham
Keyword(s):  
Palm Oil ◽  
Diesel Engines ◽  
Alternative Fuels ◽  
Internal Combustion Engines ◽  
Fossil Fuels ◽  
Volume Ratio ◽  
Emission Level ◽  
Fuel Prices ◽  
Technical Features ◽  
The Impact

The transport industry is facing problems such as the exhaustion of fossil fuels and the threat of pollution from emissions from internal combustion engines. The use of alternative fuels is considered as one of the effective solutions to address the pressure on fuel prices and environmental pollution. Using biofuels is considered an emerging solution to ensure energy security in transportation and environment friendliness. Palm oil-based biodiesel is a relatively abundant fuel source and is compatible with traditional diesel engines with little change to the engine structure. The paper focuses on the possibility of using biodiesel derived from palm oil with the volume ratio of 5%, 10% and 15% on Vikyno EV2600 engine. Thereby, it will analyze and evaluate the technical features and emission level of the engine compared to traditional diesel fuel. In addition, this study is also worth the impact of the blend ratio of palm oil-based biofuel with diesel to the PM emission level of the test engine.

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