scholarly journals IDENTIFIKASI Salmonella spp. PADA FESES SAPI PERAH DI DUSUN JUDEG DESA BABADAN, KEDIRI

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Mastuti Widianingsih ◽  
Erly Novita Dewi

ABSTRAKSelain menghasilkan daging dan susu, sapi perah juga mengekskresikan kotoran padat (feses) dan kotoran cair (urine). Feses dapat menjadi agen penyebaran Salmonella spp. yang mengakibatkan salmonellosis. Tujuan penelitian ini adalah mengidentifikasi Salmonella spp. pada feses sapi perah di Dusun Judeg, Desa Babadan, Kediri. Penelitian menggunakan 25 sampel feses sapi perah. Sampel diinokulasikan pada Selenite Broth, kemudian diinokulasi pada Salmonella Shigella Agar (SSA) , dilanjutkan pewarnaan Gram untuk mendeteksi jenis bakteri. Uji biokimia reaksi dilakukan pada hari ke-3 untuk membedakan golongan Enterobacter. Hasil penelitian menunjukkan 52% feses sapi perah di Dusun Judeg, Desa Babadan, Kecamatan Ngancar, Kediri positif terkontaminasi Salmonella spp. Kata kunci : Sapi, Feses, Salmonella spp. ABSTRACT In addition to producing meat and milk, dairy cows also excret of solid waste (feces) and liquid (urine). Feces can be a spreading agent of Salmonella spp. That caused of salmonellosis. The purpose of this research was identification Salmonella spp. on feces of dairy cow in Judeg Hamlet, Babadan Village, Ngancar District, Kediri. The research design used 25 samples. The samples were inoculated on Selenite Broth, then the inoculation to Salmonella Shigella Agar (SSA), and next the Gram staining to detect the type of bacteria. The test of biochemical reaction was performed on day 3 to differentiate the Enterobacter class. The results showed that 52% feces of dairy cow in Judeg Hamlet, Babadan Village, Ngancar District, Kediri positive contaminated of Salmonella spp. Keyword: Cow, feces, Salmonella spp.

2001 ◽  
Vol 64 (1) ◽  
pp. 3-11 ◽  
Author(s):  
S. J. WELLS ◽  
P. J. FEDORKA-CRAY ◽  
D. A. DARGATZ ◽  
K. FERRIS ◽  
A. GREEN

As part of a national study of the U.S. dairy cow population, fecal samples were collected from representative cows on 91 dairies and 97 cull dairy cow markets in 19 states. Salmonella spp. were recovered from 5.4% of milk cows, 18.1% of milk cows expected to be culled within 7 days, and 14.9% of culled dairy cows at markets. On a premise basis, Salmonella shedding in milk cows was detected on 21.1% of dairies and 66% of cull dairy cow markets. The percentage of herds with at least one cow with detectable Salmonella fecal shedding was higher during the sampling period from May through July, in herds with at least 100 milk cows, and in herds in the South region. The most common Salmonella serogroups isolated were E (30.8% of isolates) and C1 (28.6%); the most common serotypes isolated were Salmonella Montevideo (21.5% of isolates), Salmonella Cerro (13.3%), and Salmonella Kentucky (8.5%). Fecal shedding of Salmonella Typhimurium or Salmonella Typhimurium var. copenhagen was infrequent (2.8% of isolates). Most isolates (88.9%) were susceptible to all 17 antimicrobials evaluated; multiple resistance was an infrequent occurrence. This study provides information describing the distribution of Salmonella fecal shedding from dairy cows on farm and at markets and will serve as a baseline for future studies.


2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.


Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 722
Author(s):  
Bethan Cavendish ◽  
John McDonagh ◽  
Georgios Tzimiropoulos ◽  
Kimberley R. Slinger ◽  
Zoë J. Huggett ◽  
...  

The aim of this study was to characterize calving behavior of dairy cows and to compare the duration and frequency of behaviors for assisted and unassisted dairy cows at calving. Behavioral data from nine hours prior to calving were collected for 35 Holstein-Friesian dairy cows. Cows were continuously monitored under 24 h video surveillance. The behaviors of standing, lying, walking, shuffle, eating, drinking and contractions were recorded for each cow until birth. A generalized linear mixed model was used to assess differences in the duration and frequency of behaviors prior to calving for assisted and unassisted cows. The nine hours prior to calving was assessed in three-hour time periods. The study found that the cows spent a large proportion of their time either lying (0.49) or standing (0.35), with a higher frequency of standing (0.36) and shuffle (0.26) bouts than other behaviors during the study. There were no differences in behavior between assisted and unassisted cows. During the three-hours prior to calving, the duration and bouts of lying, including contractions, were higher than during other time periods. While changes in behavior failed to identify an association with calving assistance, the monitoring of behavioral patterns could be used as an alert to the progress of parturition.


2002 ◽  
Vol 2002 ◽  
pp. 92-92 ◽  
Author(s):  
T. Kokkonen ◽  
J. Taponen ◽  
S. Alasuutari ◽  
M. Nousiainen ◽  
T. Anttila ◽  
...  

In ruminants plasma leptin is increased with increasing body fatness. Leptin acts on hypothalamus to decrease food intake and increase energy expenditure. It is possible that leptin has a key role in transition from pregnancy to lactation of dairy cows. The objective of the present work was to investigate the pattern of plasma leptin concentration, as well as its relationship with other hormones and metabolites and dairy cow performance.


1998 ◽  
Vol 1998 ◽  
pp. 19-19
Author(s):  
A.R. Henderson ◽  
P.C. Garnsworthy ◽  
J.R. Newbold ◽  
P.J. Buttery

Sinclair et al. (1993) found that a diet formulated to be synchronous with regard to hourly release of nitrogen and energy increased microbial protein synthesis by 14% in sheep. Dairy cows in early lactation experience a shortfall of energy and protein, with available protein determining the overall efficiency of metabolism (MacRae and Lobley, 1986) and subsequent milk production. It is therefore necessary to maximise microbial protein yield during this period. In this study diets were designed for lactating dairy cows to contain the same feed ingredients, but to release nitrogen and energy in the rumen at different times. Rumen fermentation parameters, nutrient flows to the small intestine and production performance were investigated.


1996 ◽  
Vol 1996 ◽  
pp. 128-128
Author(s):  
J. K. Margerison ◽  
C. J. C. Phillips ◽  
T. R. Preston

Artificial rearing necessitates stressful cow-calf separation (Lefcourt and Elsasser, 1995; Hopster et al, 1995) and precipitates cross-suckling (Lidfors, 1993). Thus, suckling systems may improve the welfare of cows and calves, by alleviating 'stressful' cows-calf separation and satiating suckling motivation. The aim of this experiment was to compare behaviour in cows and calves in restricted suckling and non-suckling systems.Thirty six multiparous dairy cows and calves were divided at 4 days postpartum into three groups. The treatment groups were; non-suckled (NS) cows with calves artificially reared (AR), cows suckled by their own calf (RSM) and cows suckled by a foster calf (RSF). Suckling took place for 15 minutes following mechanical milking only.


2020 ◽  
Vol 12 (2) ◽  
pp. 663 ◽  
Author(s):  
Chao Yang Dong ◽  
Bei Bei Ma ◽  
Chun Xia LU

As the income of urban and rural residents has increased in recent decades in China, dairy products have become an important part of the Chinese diet. Therefore, keeping up with the growing demand for feed grain for dairy cows is a critical issue of feed grain security. Utilizing traditional statistical and spatial statistical methods, this study analyzes the spatio-temporal dynamics of dairy cow feed grain (DCFG) demand on the provincial, regional, and national levels across China from 1990 to 2016. Additionally, this paper explores the impacts of various factors on the spatio-temporal dynamics of DCFG demand using the Geo-Detector method. The results demonstrate that: (1) the temporal dynamics of DCFG demand can be divided into three stages of slow growth, rapid growth, and high-level stability, and the relative level of DCFG demand in the whole animal husbandry tends to decline; (2) at the regional and national levels, the spatial concentration of high DCFG demand has intensified; in particular, North China was the region where the largest demand for DCFG was localized and was increasing at the highest rate; (3) based on the hot spot analysis of provincial DCFG demand, the high and low demand provinces of DCFG have sharp characteristic contrast from north to south China; (4) the spatio-temporal dynamics of DCFG demand in China were essentially co-affected by the four groups of factors (e.g., resource endowment, feeding scale, feeding technology, and market environment), of which resource endowment and feeding scale were the dominant factors. Therefore, in the future, dairy cow feeding in China should promote grain-saving feeding technology, improve the utilization of forage, expand large-scale feeding, and create a good market environment to ensure the reasonable development and sustainability of DCFG demand.


Author(s):  
M.J. Gibb ◽  
W.E. Ivings ◽  
J.D. Sutton

In order to understand the complex changes associated with lactation in the dairy cow it is necessary to assess the contribution of the non-carcass components. It has been shown with sheep (Cowan, Robinson, Greenhalgh and McHattie, 1979) that a number of tissues may be mobilised during lactation with a redistribution of tissues particularly towards the gut and udder, and that diet may have a significant effect. Butler-Hogg, Wood and Bines (1985), using Friesian dairy cows showed changes in the weights of guts, internal fat depots (omental, mesenteric and perinephric) and liver with stage of lactation although the number of stages examined was somewhat limited. The present experiment investigates changes in non-carcass component weights at 9 points over the first 29 weeks of lactation and with 3 levels of concentrate supplementation.


2002 ◽  
Vol 2002 ◽  
pp. 220-220 ◽  
Author(s):  
M.D. Cooper ◽  
D. R. Arney ◽  
C. J. C. Phillips

The effects of high milk yields on the behaviour and welfare of the dairy cow are unclear. A high milk yield increases the need to consume sufficient fodder in an attempt to meet high nutrient demands. The failure to meet the demands may result in persistent hunger in the dairy cow having to modify her behaviour by employing various coping strategies. These modifications may help the cow overcome a state of hunger but at the expense of being unable to express other important behaviours. The objective of this study was therefore to determine whether the behaviour and welfare of the genetically high yielding dairy cow is being compromised by the increased nutritional demands of milk production, and to investigate the possibility that an increased amount of time spent in food-directed behaviours may have a detrimental effect on the time available to perform other important behaviours.


2002 ◽  
Vol 2002 ◽  
pp. 108-108
Author(s):  
J.D. Sutton ◽  
D.J. Humphries ◽  
R H Phipps ◽  
M. Witt

The complete replacement of soyabean meal by maize distillers grains (MDG) in dairy cow diets had no significant effects on milk production, rumen digestion or the supply of non-ammonia N to the duodenum (Sutton et al., 2000; Phipps et al., 2001). The present experiment was designed to extend this work by examining the response when barley (BDG) or wheat (WDG) distillers grains replaced soyabean meal.


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