Total Phenolic and Flavonoid Contents and Antioxidant Activity of Kembang Bulan Leaves (Tithonia diversifolia (Hemsley) A. Gray)

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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Chemical Composition, Production of Secondary Metabolites and Antioxidant Activity in Coffee Cultivars Susceptible and Partially Resistant to Bacterial Halo Blight

Plants ◽

10.3390/plants10091915 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1915

Author(s):

Joyce Alves Goulart da Silva ◽

Mário Lúcio Vilela de Resende ◽

Ingridy Simone Ribeiro ◽

Adriene Ribeiro Lima ◽

Luiz Roberto Marques Albuquerque ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Secondary Metabolites ◽

Pseudomonas Syringae ◽

Total Phenolic ◽

Leaf Extracts ◽

Defense Strategies ◽

Halo Blight ◽

Phenolic Profiles ◽

Phenolic And Flavonoid

Coffee production is one of the main agricultural activities in Brazil, and several coffee cultivars with disease resistance have already been developed. The secondary metabolites produced by plants are closely associated with defense strategies, and the resistance of coffee cultivars to bacterial halo blight (BHB) can be related to these compounds. Therefore, this study aims to compare a partially resistant coffee cultivar (Iapar-59) and a susceptible cultivar (Mundo Novo 376/4) to BHB (Pseudomonas syringae pv. garcae) in relation to the chemical composition and antioxidant activity of the leaf extracts. In addition, this study determined the total phenolic and flavonoid contents and phenolic profiles of the Iapar-59 leaf extracts of plants inoculated with P. syringae pv. garcae. The Iapar-59 extract showed a higher content of phenolic compounds and flavonoids than the Mundo Novo 376/4 extract. Both cultivars contained gallic, chlorogenic and caffeic acids; however, the highest contents were quantified in the Iapar-59 cultivar. The leaf extracts from the Iapar-59 cultivar exhibited higher antioxidant activity. Higher concentrations of gallic, caffeic and chlorogenic acids and the presence of vanillin were detected in the extract of cultivar Iapar-59 inoculated with P. syringae pv. garcae.

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Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel)

Food Research ◽

10.26656/fr.2017.3(6).135 ◽

2019 ◽

pp. 734-740

Author(s):

Nur S. ◽

F. Mubarak ◽

C. Jannah ◽

D.A. Winarni ◽

D.A. Rahman ◽

...

Keyword(s):

Antioxidant Activity ◽

Crude Extract ◽

Iron Reduction ◽

Colorimetric Method ◽

Total Phenolic ◽

Toxicity Profile ◽

Flavonoid Content ◽

Ic50 Value ◽

Phenolic And Flavonoid ◽

Phenolic And Flavonoid Compounds

Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically as an anticancer and antidote materials by Dayaknese in West Kutai, with limited scientific study. Thus, this research had been conducted to determine and evaluate the total phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile. The research used several extracts’ solvents including extraction with ethanol (crude extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed through colorimetric method to determine the phenolic and flavonoid total; with DPPH and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the toxicity activity. The results showed that the EF fraction provided the strongest antioxidant activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of 387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and the total phenolic content (r2 = 0.970 - 0.974) but little correlation in total flavonoid (r2 = 0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the paku atai tuber can be potentially developed as a natural antioxidant and anticancer material.

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Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

E3S Web of Conferences ◽

10.1051/e3sconf/202133208005 ◽

2021 ◽

Vol 332 ◽

pp. 08005

Author(s):

Aminah Dalimunthe ◽

Dewi Pertiwi ◽

Mahatir Muhammad ◽

Denny Satria

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethanol Extract ◽

The Body ◽

Total Flavonoid ◽

Total Phenolic ◽

Antioxidant Power ◽

Litsea Cubeba ◽

Ferric Reducing Antioxidant Power ◽

Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

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Variability of Total Phenolic and Flavonoid Content and Antioxidant Activity Among 20 Curcuma aeruginosa Roxb. Accessions of Indonesia

Asian Journal of Biochemistry ◽

10.3923/ajb.2016.142.148 ◽

2016 ◽

Vol 11 (3) ◽

pp. 142-148 ◽

Cited By ~ 5

Author(s):

Waras Nurcholis ◽

Nurul Khumaida ◽

Muhamad Syukur ◽

Maria Bintang

Keyword(s):

Antioxidant Activity ◽

Total Phenolic ◽

Flavonoid Content ◽

Phenolic And Flavonoid

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Enhanced Antioxidant Activity for Apple Juice Fermented with Lactobacillus plantarum ATCC14917

Molecules ◽

10.3390/molecules24010051 ◽

2018 ◽

Vol 24 (1) ◽

pp. 51 ◽

Cited By ~ 29

Author(s):

Zhongxi Li ◽

Jing Teng ◽

Yilu Lyu ◽

Xiaoqian Hu ◽

Yueliang Zhao ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Lactobacillus Plantarum ◽

Apple Juice ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Phenolic Profile ◽

Chemical Models ◽

Phenolic And Flavonoid

The present study examined the effect of Lactobacillus plantarum ATCC14917 fermentation on the chemical composition and antioxidant activity of apple juice. Apple juice was fermented and examined of its antioxidant activity using chemical models and cellular antioxidant assay. Furthermore, the chemical composition of fermented apple juice was characterized by LC-MS/MS. Lactobacillus plantarum ATCC14917 fermentation showed an increase in DPPH and ABTS radical scavenging activity as well as cellular antioxidant activity of apple juice. However, fermentation decreased the total phenolic and flavonoid content. Subsequent LC-MS/MS analysis of the phenolic profile indicated that the content of 5-O-caffeoylquinic acid (5-CQA), quercetin, and phloretin with strong antioxidant activity was increased significantly after fermentation. The modified phenolic composition may contribute to the increased antioxidant activity of fermented apple juice. Our findings showed that Lactobacillus plantarum ATCC14917 fermentation may be an efficient way to enhance the bioavailability of phenolic compounds and to protect cells from oxidative stress.

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Total Phenolic and Flavonoid Content and Biological Activities of Extracts and Isolated Compounds of Cytisus villosus Pourr.

Biomolecules ◽

10.3390/biom9110732 ◽

2019 ◽

Vol 9 (11) ◽

pp. 732 ◽

Cited By ~ 2

Author(s):

Farida Larit ◽

Francisco León ◽

Samira Benyahia ◽

Stephen Cutler

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Nitric Oxide Synthase ◽

Total Phenolic ◽

Flavonoid Content ◽

Pure Compounds ◽

Ic50 Values ◽

Anti Inflammatory ◽

Oxide Synthase ◽

Phenolic And Flavonoid

The aim of this study was to evaluate the total phenolic and flavonoid content, and the in vitro antioxidant, anti-inflammatory, antibacterial, antifungal, antimalarial, cytotoxicity, and antiprotozoal activities of the Algerian plant Cytisus villosus Pourr. (Syn. Cytisus triflorus L’Hérit.). Additionally, the radioligand displacement affinity on opioid and cannabinoid receptors was assessed for the extracts and isolated pure compounds. The hydro alcoholic extract of the aerial part of C. villosus was partitioned with chloroform (CHCl3), ethyl acetate (EtOAc), and n-butanol (n-BuOH). The phenolic content of the C. villosus extracts was evaluated using a modified Folin–Ciocalteau method. The total flavonoid content was measured spectrometrically using the aluminum chloride colorimetric assay. The known flavonoids genistein (1), chrysin (2), chrysin-7-O-β-d-glucopyranoside (3), and 2″-O-α-l-rhamnosylorientin (4) were isolated. The antioxidant activities of the extracts and isolated compounds were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DDPH) and cellular antioxidant activity (CAA) assays. The plant extracts showed moderate antioxidant activity. EtOAc and n-BuOH extracts showed moderate anti-inflammatory activity through the inhibition of induced nitric oxide synthase (iNOS) with IC50 values of 48 and 90 µg/mL, respectively. The isolated pure compounds 1 and 3 showed good inhibition of Inducible nitric oxide synthase (iNOS) with IC50 values of 9 and 20 µg/mL, respectively. Compounds 1 and 2 exhibited lower inhibition of Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) with IC50 values of 28 and 38 µg/mL, respectively. Furthermore, the extracts and isolated pure compounds have been shown to exhibit low affinity for cannabinoid and opioid receptors. Finally, n-BuOH extract was a potent inhibitor of Trypanosoma brucei with IC50 value of 7.99 µg/mL and IC90 value of 12.61 µg/mL. The extracts and isolated compounds showed no antimicrobial, antimalarial nor antileishmanial activities. No cytotoxic effect was observed on cancer cell lines. The results highlight this species as a promising source of anti-inflammatory and antitrypanosomal agents.

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In Vitro Callus Culture of Heliotropium indicum Linn. for Assessment of Total Phenolic and Flavonoid Content and Antioxidant Activity

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-014-1235-1 ◽

2014 ◽

Vol 174 (8) ◽

pp. 2897-2909 ◽

Cited By ~ 11

Author(s):

Muthusamy Senthil Kumar ◽

Shibani Chaudhury ◽

Srinivasan Balachandran

Keyword(s):

Antioxidant Activity ◽

Callus Culture ◽

Total Phenolic ◽

Flavonoid Content ◽

Heliotropium Indicum ◽

Phenolic And Flavonoid

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Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

Journal of Scientific Research ◽

10.3329/jsr.v6i3.17586 ◽

2014 ◽

Vol 6 (3) ◽

pp. 543-552 ◽

Cited By ~ 1

Author(s):

N. Jahan ◽

M. S. Parvin ◽

A. Khan ◽

N. Das ◽

M. S. Islam ◽

...

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Soluble Fraction ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Deciduous Tree ◽

Total Phenolic ◽

Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

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