Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel)

Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically as an anticancer and antidote materials by Dayaknese in West Kutai, with limited scientific study. Thus, this research had been conducted to determine and evaluate the total phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile. The research used several extracts’ solvents including extraction with ethanol (crude extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed through colorimetric method to determine the phenolic and flavonoid total; with DPPH and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the toxicity activity. The results showed that the EF fraction provided the strongest antioxidant activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of 387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and the total phenolic content (r2 = 0.970 - 0.974) but little correlation in total flavonoid (r2 = 0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the paku atai tuber can be potentially developed as a natural antioxidant and anticancer material.

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Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

Journal of Nepal Chemical Society ◽

10.3126/jncs.v40i0.27275 ◽

2019 ◽

Vol 40 ◽

pp. 30-35

Author(s):

Ishwor Pathak ◽

Muna Niraula

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Colorimetric Method ◽

Radical Scavenging ◽

Hexane Extract ◽

Ocimum Sanctum ◽

Total Phenolic ◽

Flavonoid Content ◽

Ic50 Value ◽

Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

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Comparative Evaluation of Crude Extract Fractions of the Whole Plant of Taxillus heyneanus and Dalechampia indica for Antioxidant Activity, Total Phenolic and Flavonoid Content

IOSR Journal of Pharmacy (IOSRPHR) ◽

10.9790/3013-0705015360 ◽

2017 ◽

Vol 07 (05) ◽

pp. 53-60

Author(s):

S. Sindhura ◽

Dr. M. Chinna Eswaraiah

Keyword(s):

Antioxidant Activity ◽

Crude Extract ◽

Comparative Evaluation ◽

Total Phenolic ◽

Flavonoid Content ◽

Whole Plant ◽

Phenolic And Flavonoid

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Korelasi Antara Kadar Total Flavonoid dan Fenolik dari Ekstrak dan Fraksi Daun Jati Putih (Gmelina Arborea Roxb.) Terhadap Aktivitas Antioksidan

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2019.v5.i1.12034 ◽

2019 ◽

Vol 5 (1) ◽

pp. 33-42

Author(s):

Syamsu Nur ◽

Fitriyanti Jumaetri Sami ◽

Akbar Awaluddin ◽

Mutiara Indah Ayu Afsari

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Colorimetric Method ◽

Total Flavonoid ◽

Total Phenolic ◽

Phytochemical Screening ◽

Gmelina Arborea ◽

Flavonoid Content ◽

Coefficient Corrélation ◽

Phenolic And Flavonoid

This experiment aims to determine the correlation of total phenolic and flavonoid content of jati putih leaves fraction (Gmelina arborea Roxb.) towards Antioxidant activity . Sample was extracted by maceration method using ethanol 70% to obtain the ethanol extract (EE), followed by liquid-liquid extraction method to obtain fraction of ethyl acetate (EA) and n-Hexane (EH). The phytochemical screening and determination of total phenolic and flavonoid content were done by colorimetric method. Antioxidant activity were done by DPPH, FRAP and ABTS methods. Phytochemical screening showed positive results for flavonoids, phenolic and saponins. The largest total phenolic content was found on EA (11,59 µg/ml ± 0,3 %b/b EAG) and the largest total flavonoid content was on EA (3,88 µg/ml ± 0,02 %b/b EK). The total phenolic and flavonoid content of Jati putih leaves has a correlation with antioxidant activity. The coefficient correlation of activity on reducingDPPH radical was 56,7% (total of phenolic content) and 57,8% (total of flavonoid content) and on iron reduction power in FRAP method was 99,9% (total of phenolics and flavonoids content). The relationship with the activity in reducing radical ABTS obtained coefficient correlation of 57,0% and 58,1% for total phenolic and flavonoids contents, respectively.

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Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200238 ◽

2019 ◽

Vol 20 (2) ◽

pp. 576-580

Author(s):

HENNY NURHASNAWATI ◽

REKSI SUNDU ◽

SAPRI SAPRI ◽

RISA SUPRININGRUM ◽

HARLINDA KUSPRADINI ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Colorimetric Method ◽

Ethanol Extract ◽

Total Phenolic ◽

Indigenous Species ◽

Flavonoid Content ◽

East Kalimantan ◽

Phenolic And Flavonoid

Abstract. Nurhasnawati H, Sundu R, Sapri, Supriningrum R, Kuspradini H, Arung ET. 2019. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas 20: 576-580. This study aimed to determine the total phenolic and flavonoids content and antioxidant activity of ethanol extract of several indigenous species of ferns in East Kalimantan. Total phenolic content was determined by Folin-Ciocalteau method and flavonoid content was measured by colorimetric method. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The result of photochemical screening indicated that the leaves of Plagiogyria pycnophylla, Plagiogyria glauca, and Stenochlaena palustris contained alkaloid, flavonoid, tannin, saponin, and steroid while Acrostichum aureum contained flavonoid, tannin, saponin and steroid. The result showed extract Acrostichum aureum had the highest total phenolic content (366.4573 ± 2.2117 mg GAE.g-1), flavonoid content (228.6087 ± 2.2548 mg QE.g-1), and very strong antioxidant activity with IC50 value 29.5303 ppm. There is positive correlation between total phenolic content, flavonoid with antioxidant activity.

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Differences in Parts of Purslane Plants (Portulaca grandiflora Hook.) To Total Phenolic and Flavonoid Content and Antioxidant Activity

Current Biochemistry ◽

10.29244/cb.6.3.1 ◽

2020 ◽

Vol 7 (1) ◽

pp. 10

Author(s):

Husnawati ◽

Ukhradiya Magharaniq Safira Purwanto ◽

Aulia Ayu Rispriandari

Keyword(s):

Antioxidant Activity ◽

The Body ◽

Total Phenolic ◽

Flavonoid Content ◽

Phytochemical Content ◽

Plant Parts ◽

Ic50 Value ◽

Portulaca Grandiflora ◽

Flower Organs ◽

Phenolic And Flavonoid

Antioxidant are needed to prevent free radical inside the body and oxidative stress that promotes the aging process and various degenerative diseases. Portulaca grandiflora Hook. is known to have potential as antioxidant. Differences in plant parts can cause differences in phytochemical content, which can affect its antioxidant activity. This study aims to measure total phenolic and flavonoid content in leaf, old stem, young stem, and flower organs of purslane plant (Portulaca grandiflora Hook.) which is extracted using ethanol 96% and determined the antioxidant activity of the extract with DPPH methode. The result showed that each plant organ of purslane contains different concentration of phenolic and flavonoid compounds. The highest total phenolic and flavonoid content was found in the leaves( 113.26 ± 3.85 mg GAE/g and 97.99 ± 1.28 mg QE/g), but the highest antioxidant activity was found in the old stem with IC50 value 122.15 ± 1.30 ppm (classified as medium antioxidant). Keywords: Antioxidant, Flavonoid, Phenolic, Portulaca grandiflora Hook.

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Antioxidant activity of Galium mollugo L. extracts obtained by different recovery techniques

Hemijska industrija ◽

10.2298/hemind120314042m ◽

2013 ◽

Vol 67 (1) ◽

pp. 89-94 ◽

Cited By ~ 8

Author(s):

Petar Milic ◽

Ljiljana Stanojevic ◽

Katarina Rajkovic ◽

Slavica Milic ◽

Vesna Nikolic ◽

...

Keyword(s):

Antioxidant Activity ◽

Colorimetric Method ◽

Total Flavonoid ◽

Total Phenolic ◽

Extraction Techniques ◽

Aerial Parts ◽

Recovery Techniques ◽

Extractive Substances ◽

Phenolic And Flavonoid ◽

Phenolic And Flavonoid Compounds

The yield of extractive substances, antioxidant activity, as well as total phenolic and total flavonoid contents of aqueous-ethanolic extracts obtained from aerial parts of Galium mollugo L. by different extraction techniques (maceration, reflux and ultrasonic extraction) were reported. The antioxidant activity of extracts was tested by measuring their ability to scavenge a stable DPPH free radical, while the total phenolic and total flavonoid contents were determined according to the Folin-Ciocalteu procedure and a colorimetric method, respectively. The Duncan?s multiple range tests were used to evaluate if there were significant differences among yields of extractive substances, total phenolics, total flavonoids and EC50 values for the extracts obtained by different extraction techniques. The extracts obtained by the reflux extraction contained higher amounts of extractive substances, as well as phenolic and flavonoid compounds, and showed a better antioxidant activity than those obtained by the two other recovering techniques.

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POTENTIAL ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ARECA NUT (ARECA CATECHU LINN.) EXTRACT IN HUMAN ORAL SQUAMOUS CELL CARCINOMA AND KERATINOCYTE CELLS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i10.20287 ◽

2017 ◽

Vol 10 (10) ◽

pp. 286 ◽

Cited By ~ 6

Author(s):

Liza Meutia Sari ◽

Gus Permana Subita ◽

Elza Ibrahim Auerkari

Keyword(s):

Antioxidant Activity ◽

Total Phenolic ◽

Cytotoxic Activities ◽

Hacat Cells ◽

Areca Nut ◽

Flavonoid Content ◽

Flavonoid Compounds ◽

Areca Catechu ◽

Phenolic And Flavonoid ◽

Phenolic And Flavonoid Compounds

Objectives: Areca (betel, Areca catechu) nuts have been reported to show high contents of phenolic and flavonoid compounds with associated antioxidant activity. This study was undertaken to assess the anticancer potential of areca nut extract on human squamous carcinoma (HSC)-3, HSC-2, and human keratinocyte (HaCat) cancer cell lines, and to elucidate the mechanism of its action.Methods: In this study, the areca nut extract was prepared in 96% ethanol and analyzed for the total phenolic and flavonoid content, 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, and 5-(3-carboxymethoxyphenyl)-2-(4,5-dimenthylthiazoly)-3-(4-sulfophenyl)tetrazolium inner salt assay.Results: The indicated total phenolic content of the areca nut extract was 80.3 mg tannic acid equivalent/g and total flavonoid content 238.5 mg catechin equivalent/g. The extract showed good scavenging activity against DPPH radical (efficient concentration 15.95±0.84 μg/ml). The extract showed higher toxicity on HSC-3 than HSC-2 cells, IC50 was 164.06 μg/ml in HSC-3 versus 629.50 μg/ml in HSC-2. The extract had no cytotoxic effect on HaCat cells.Conclusions: The antioxidant activity of the areca nut extract might be associated with the presence of high content of phenolic and flavonoid compounds. The extract showed selective cytotoxicity against HSC-3 and HSC-2 cells but not on HaCat cells. Instead, the extract induced high rate of proliferation in HaCat cells.

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Aktivitas Antioksidan Dari Ekstrak Etanol Herba Poguntano (Picria Fel-Terrae Lour.) Secara In Vitro

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i1.77 ◽

2018 ◽

Vol 1 (1) ◽

pp. 218-223

Author(s):

Nevi Hayati Utami ◽

Suryadi Achamad ◽

Panal Sitorus

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging ◽

Ethanol Extract ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Ic50 Value ◽

Phenolic And Flavonoid

Latar Belakang: Antioksidan adalah senyawa yang mampu menangkal atau meredam efek negatif oksidan dalam tubuh, bekerja dengan cara mendonorkan satu elektronnya kepada senyawa yang bersifat oksidan sehingga aktivitas senyawa oksidan tersebut dapat dihambat. Herba poguntano (Picria fel-terrae Lour.) digunakan sebagai obat antidiabetes dan telah dilaporkan mengandung senyawa kimia yang terdiri dari alkaloid, saponin, flavonoid, dan polifenol yang merupakan metabolit sekunder yang bersifat antioksidan. Tujuan Penelitian: Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan serta mengetahui kandungan fenolat dan flavonoid total dari ekstrak etanol herba poguntano. Metode Penelitian: Ekstrak diperoleh secara maserasi dengan pelarut etanol 96%, lalu dipekatkan dengan alat rotary evaporator hingga didapatkan ekstrak kental. Ekstrak diuji dengan metode pemerangkapan radikal bebas DPPH (1,1-diphenyl-2-picrylhydrazyl) serta mengukur kandungan fenolat total dan flavonoid total dengan mengukur absorbansi menggunakan spektrofotometri uv-vis yang diinkubasi berturut-turut selama 60, 90 dan 40 menit pada panjang gelombang 516, 775 dan 432 nm. Hasil: Hasil pengukuran aktivitas antioksidan dengan metode pemerangkapan radikal bebas DPPH dari ekstrak etanol herba poguntano menunjukkan kekuatan antioksidan dalam kategori sangan kuat dengan nilai IC50 21.79 ppm dan kuersetin menunjukkan kekuatan antioksidan dalam kategori sangat kuat dengan nilai IC50 4.9 ppm. Hasil pengukuran kandungan fenolat total dengan metode Folin-Ciocalteau menunjukkan angka 66.29 mg GAE/g ekstrak serta hasil pengukuran kandungan flavonoid total menunjukkan angka 19.26 mg QE/g ekstrak. Kesimpulan: Ekstrak etanol herba poguntano mempunyai aktivitas antioksidan dengan kategori sangat kuat serta memiliki kandungan fenolat total yang tinggi dibandingkan dengan kandungan flavonoid total. Background: Antioxidants are compounds capable of counteracting or reducing the negative effects of oxidants in the body. Antioxidants work by donating an electron to an oxidant compound so that the activity of the oxidant compound can be inhibited. Poguntano (Picriafel-terrae Lour) is used as an antidiabetic drug and has been reported to contain chemical compounds, , as secondary metabolites that have antioxidant properties. Objective: This study aimed to determine antioxidant activity and total phenolic and flavonoid content of the ethanol extract of poguntano. Research Method: . The dried materials were macerated using 96% ethanol and then it was concentrated using rotary evaporator to obtain crude extract. The extract was tested by DPPH free radical scavenging method (1,1-diphenyl-2-picrylhydrazyl) and the total phenolic and flavonoid content was determinedby measuring absorbance using uv-vis spectrophotometry which was incubated successively for 60, 90 and 40 minutes at wavelength 516, 775 and 432 nm. Results: The evaluationof antioxidant activity using DPPH free radical scavenging method ofethanol extract of poguntano showed the antioxidant activitywas in very strong category with IC50 value of 21.79 ppm and quercetin showed the antioxidant activitywas in very strong category with IC50 value of 4.9 ppm. The result of the measurement of total phenolic content by the Folin-Ciocalteu method was66.29 mg GAE/g extract and the result of measurements of total flavonoid content was19.26 mg QE/g extract. Conclusion: Ethanol extract of poguntanohas antioxidant activity in a very strong category and it has higher total phenolic content compared with total flavonoid content.

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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