Chemical Composition, Production of Secondary Metabolites and Antioxidant Activity in Coffee Cultivars Susceptible and Partially Resistant to Bacterial Halo Blight

Coffee production is one of the main agricultural activities in Brazil, and several coffee cultivars with disease resistance have already been developed. The secondary metabolites produced by plants are closely associated with defense strategies, and the resistance of coffee cultivars to bacterial halo blight (BHB) can be related to these compounds. Therefore, this study aims to compare a partially resistant coffee cultivar (Iapar-59) and a susceptible cultivar (Mundo Novo 376/4) to BHB (Pseudomonas syringae pv. garcae) in relation to the chemical composition and antioxidant activity of the leaf extracts. In addition, this study determined the total phenolic and flavonoid contents and phenolic profiles of the Iapar-59 leaf extracts of plants inoculated with P. syringae pv. garcae. The Iapar-59 extract showed a higher content of phenolic compounds and flavonoids than the Mundo Novo 376/4 extract. Both cultivars contained gallic, chlorogenic and caffeic acids; however, the highest contents were quantified in the Iapar-59 cultivar. The leaf extracts from the Iapar-59 cultivar exhibited higher antioxidant activity. Higher concentrations of gallic, caffeic and chlorogenic acids and the presence of vanillin were detected in the extract of cultivar Iapar-59 inoculated with P. syringae pv. garcae.

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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Enhanced Antioxidant Activity for Apple Juice Fermented with Lactobacillus plantarum ATCC14917

Molecules ◽

10.3390/molecules24010051 ◽

2018 ◽

Vol 24 (1) ◽

pp. 51 ◽

Cited By ~ 29

Author(s):

Zhongxi Li ◽

Jing Teng ◽

Yilu Lyu ◽

Xiaoqian Hu ◽

Yueliang Zhao ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Lactobacillus Plantarum ◽

Apple Juice ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Phenolic Profile ◽

Chemical Models ◽

Phenolic And Flavonoid

The present study examined the effect of Lactobacillus plantarum ATCC14917 fermentation on the chemical composition and antioxidant activity of apple juice. Apple juice was fermented and examined of its antioxidant activity using chemical models and cellular antioxidant assay. Furthermore, the chemical composition of fermented apple juice was characterized by LC-MS/MS. Lactobacillus plantarum ATCC14917 fermentation showed an increase in DPPH and ABTS radical scavenging activity as well as cellular antioxidant activity of apple juice. However, fermentation decreased the total phenolic and flavonoid content. Subsequent LC-MS/MS analysis of the phenolic profile indicated that the content of 5-O-caffeoylquinic acid (5-CQA), quercetin, and phloretin with strong antioxidant activity was increased significantly after fermentation. The modified phenolic composition may contribute to the increased antioxidant activity of fermented apple juice. Our findings showed that Lactobacillus plantarum ATCC14917 fermentation may be an efficient way to enhance the bioavailability of phenolic compounds and to protect cells from oxidative stress.

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Characterization of Chemical Compounds and Antioxidant Activity of Centaurea solstitialis sp. schouwii (DC.) Q. et S. (Asteraceae)

Current Bioactive Compounds ◽

10.2174/1573407215666190213125259 ◽

2020 ◽

Vol 16 (5) ◽

pp. 618-626

Author(s):

Lamia Aliouche ◽

Paul Mosset ◽

Francisco León ◽

Ignacio Brouard ◽

Samir Benayache ◽

...

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Sesquiterpene Lactone ◽

Antioxidant Activities ◽

Centaurea Solstitialis ◽

Total Phenolic ◽

Butanol Extract ◽

Aerial Parts ◽

Phenolic And Flavonoid

Background: The antioxidant activity and the total phenolic and flavonoid contents of the derived extracts (chloroform, ethyl acetate and n-butanol) of the 70% hydroalcoholic extract of the aerial parts of Centaurea solstitialis growing in Algeria was assessed. The active extracts were selected for phytochemical investigations. Methods: The antioxidant capabilities of the extracts were assessed using 1, 1-diphenyl-2- picrylhydrazyl radical (DPPH•) scavenging and Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assays. Butylhydroxyanisole (BHA), butylhydroxytoluene (BHT) and α –tocopherol were used as positive controls. The total phenolic content and total flavonoid content of the extracts were determined as gallic acid equivalents and quercetin equivalents, respectively. Chromatographic methods were used to isolate the secondary metabolites and spectrometric and spectroscopic methods were used to determine their chemical structures. Results: The ethyl acetate extract exhibited the highest antioxidant activities followed by the n-butanol extract. The highest phenolic and flavonoid contents were found in the n-butanol extract. Phytochemical study of the ethyl acetate and n-butanol extracts led to the isolation of an undescribed guaianolide named 3-(4-hydroxybenzoyl)-cynaratriol and a known sesquiterpene lactone along with three known flavonoid glycosides. Their structures were established by spectral analyzes mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and 1D and 2D nuclear magnetic resonance experiments. Conclusion: The extracts of aerial parts of C. solstitialis showed significant antioxidant activities. An undescribed sesquiterpene lactone and four known secondary metabolites were isolated from the most active extracts.

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Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

Current Traditional Medicine ◽

10.2174/2215083804666181012123333 ◽

2019 ◽

Vol 5 (2) ◽

pp. 159-167 ◽

Cited By ~ 2

Author(s):

Vinod Kumar Gautam ◽

Mannu Datta ◽

Ashish Baldi

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Seasonal Variations ◽

Summer Season ◽

Antioxidant Potential ◽

Total Phenolic ◽

Spring Season ◽

Phenolic Contents ◽

Aerial Parts ◽

Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

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Quantification of phenolics, flavonoids and antioxidant activity of Tamarindus indica from selected areas in Tanzania

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f160103 ◽

2018 ◽

Vol 16 (1) ◽

pp. 22-28

Author(s):

MOURICE MBUNDE ◽

ROBINSON H. MDEGELA ◽

H.S. LASWAI ◽

F.P. MABIKI

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Tamarindus Indica ◽

Leaf Extracts ◽

Fruit Extracts ◽

Significant Difference ◽

Phenolic And Flavonoid ◽

Quantification Of Phenolics

Mbunde M, Mdegela RH, Laswai HS, Mabiki FP. 2018. Quantification of phenolics, flavonoids and antioxidant activity of Tamarindus indica from selected areas in Tanzania. Biofarmasi J Nat Prod Biochem15: 22-28. The objective of this study was to establish the quantities and antioxidant activity in fruits and leaves of Tamarindus indica L. collected from three agro-ecological zones of Tanzania represented by Morogoro, Tanga and Dodoma regions. Samples were examined for their total phenolic and flavonoid contents as well as their antioxidant activity. The total phenolic content showed significant difference in all extracts of the fruits and leaves and ranged from 1994.4±530.77 to 17874.67±5234 mg GAE/100 g. Similarly, the total flavonoid content in tamarind leaf and fruit extracts ranged from 880±609.45 to 11483.11±2559.67 mg CE /100 g dry weight. There was a significant difference between the antioxidant activity in the leaf (54.39±0.13%) and fruit extracts (40.11±0.03%). Tamarind leaf extracts exhibited significantly higher radical scavenging activity than fruit extracts. The antioxidant activity in fruit extracts expressed in percentage ranged between 29.27±0.06% and 40.11±0.03% while in leaf extracts the activity ranged from 22.33±0.08% to 54.39±0.13%. The radical scavenging activity from Coastal leaf extracts had the highest activity followed by Eastern leaf extracts and lastly Central leaf extracts. In the fruit samples, the highest activity was shown by Coastal fruit extracts followed by Central fruit extracts and Eastern fruit extract being the least active. The values in Ferric reducing power (FRAP) assay ranged between 6968±3655.91 µM Fe (II)/g and 76822.67±23259.9 µM Fe (II)/g for leaves and fruits dry mass respectively. These values correspond to the antioxidant activity which positively correlated with the total phenolic and flavonoid contents. Geographical location and climatic conditions have been reported to have enormous effects on the amount and activity of antioxidants available in both tamarind leaves and fruits. Findings from the study indicated that tamarind can be utilized as a cheap source of antioxidants. However, more agronomic studies should be considered to confirm the effects of agroecological differences on antioxidant activity.

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IN VITRO ANTIOXIDANT ACTIVITY OF VARIOUS LEAF EXTRACTS OF CANTHIUM COROMANDELICUM(BURM.F.) ALSTON.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.17221 ◽

2017 ◽

Vol 10 (5) ◽

pp. 214

Author(s):

R.shabi Ruskin

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging ◽

Total Phenolic ◽

Peroxide Radical ◽

Leaf Extracts ◽

Phenolic And Flavonoid ◽

In Vitro Antioxidant Activity

Objectives: The objective of this study was to estimate the total phenolic and flavonoid contents and to explore the antioxidant potential of various leaf extracts (chloroform, ethyl acetate, and ethanol) of Canthium coromandelicum, which is considered traditionally as an important medicinal plant.Methods: Antioxidant properties of the extracts were assessed using 1, 1- diphenyl -2- picrylhydrazyl and hydrogen peroxide radical scavenging assays for ascorbic acid equivalents. The total phenolic and flavonoid contents were also investigated to determine their correlation with the antioxidant activity of the leaf extracts and expressed in Gallic acid and quercetin equivalents, respectively.Results: The results showed that the content of total phenols and flavonoids was found to be high in ethyl acetate extract which was recorded as 61.02±1.30 mg Gallic acid equivalent (GAE/g) and 81.72±0.61 QE/g, respectively. Compared to other extracts, ethyl acetate leaf extract was found to possess high antioxidant activity at p<0.05 level, with high percentage of inhibition at 100 μg/ml concentration (82.70%) toward hydrogen peroxide radical scavenging with IC50 value 62.94 μg/ml. Statistically, two-tailed Pearson’s correlation showed strong positive correlations between hydrogenperoxide radical scavenging activity and total phenolic contents (TPC) (r=1.000) at p<0.05 level.Conclusion: The results obtained in this study clearly signifies that the ethyl acetate leaf extract of C. coromandelicum has high content of total phenols which are correlated to its antioxidant activity and thus has the potential to use as a source of natural antioxidants and can be explored as a therapeutic agent in free radical induced diseases.Keywords: Canthium coromandelicum, In-vitro antioxidant activity, Ethyl acetate extract, Pearson’s correlation, 1, 1- diphenyl -2- picrylhydrazyl, Hydrogen peroxide, Nitric oxide, Superoxide.

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Chemical Composition and In Vitro Antioxidant and Antimicrobial Activities of Marrubium vulgare L.

The Scientific World JOURNAL ◽

10.1155/2021/7011493 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Ibrahim Mssillou ◽

Abdelkrim Agour ◽

Noureddine Hamamouch ◽

Badiaa Lyoussi ◽

Elhoussine Derwich

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Antimicrobial Activities ◽

Total Phenolic ◽

Leaf Extracts ◽

Total Antioxidant ◽

Marrubium Vulgare ◽

Antioxidant And Antimicrobial Activities

In this study, the polyphenol content and the antioxidant and antimicrobial activities of hydroethanolic (MVE) and hydroacetonic (MVA) leaf extracts of Marrubium vulgare L. were examined. The results indicated that the total phenolic content was higher in MVA (112.09 ± 4.77 mg GAE/DW) compared to MVE extract (98.77 ± 1.68 mg GAE/DW). The total flavonoid content was also higher in MVA extract (21.08 ± 0.38 mg QE/g DW) compared to MVE (17.65 ± 0.73 mg QE/g DW). Analysis of the chemical composition revealed the presence of 13 compounds with a total of 96.14%, with the major compound being malic acid (22.57%). Both extracts possess a good total antioxidant activity. DPPH and FRAP assays indicated that the MVE extract possesses a better antioxidant activity, with IC50 = 52.04 µg/mL ± 0.2 and EC50 of 4.51 ± 0.5 mg/mL, compared to MVA extract (IC50 = 60.57 ± 0.6 µg/mL and EC50 of 6.43 ± 0.0411 mg/mL). Moreover, both extracts exhibited strong antimicrobial activity against certain nosocomial strains as indicted by the MIC values, which ranged between 0.93 mg/mL and 10 mg/mL. Taken together, these results reveal the importance of M. vulgare as a natural antioxidant with important antimicrobial activity.

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Total phenolic content and antioxidant activity in eight cowpea ( Vigna unguiculata) genotypes

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/924/1/012047 ◽

2021 ◽

Vol 924 (1) ◽

pp. 012047

Author(s):

E Yusnawan ◽

A Inayati ◽

Y Baliadi

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Plant Growth ◽

Vigna Unguiculata ◽

Seed Coat ◽

Phenolic Content ◽

Total Phenolic ◽

Phenolic Contents ◽

Extraction Solvents ◽

Phenolic And Flavonoid

Abstract Cowpea is considered as one of the underutilized legumes which is rich in secondary metabolites. These metabolites can be detected during the plant growth and the contents increase especially after the plants are stressed both abiotic and biotic. This study aimed to determine total phenolic and flavonoid contents as well as antioxidant activity in seeds of eight cowpea genotypes. Two extraction solvents (70% acetone and 70% acidified acetone) were used to extract the secondary metabolites in those cowpea genotypes. Total flavonoid contents of those genotypes extracted in 70% acetone and 70% acidified acetone were in the range from 7.28 to 10.90 mg CE/g and 7.84 to 11.58 mg CE/g, whereas total phenolic contents were from 15.20 to 19.99 mg GAE/g and 15.03 to 21.15 mg GAE/g. Inhibition percentages of antioxidant activity were in the range from 46.90 % to 59.46 % and 50.71 % to 63.81 % for 70% acetone and 70% acidified acetone, respectively. Both solvents were effective to extract those secondary metabolites. Flavonoid and phenolic contents of MLGU 0239 were the highest among other genotypes, which were 11.58 mg CE/g and 21.15 GAE/g and antioxidant activity was 63.81 % inhibition after being extracted with 70% acidified acetone. This study observed the variation of the secondary metabolites among eight cowpea genotypes with different seed coat colours.

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Antioxidant activity, α-glucosidase inhibition and phytochemical profiling of Hyophorbe lagenicaulis leaf extracts

PeerJ ◽

10.7717/peerj.7022 ◽

2019 ◽

Vol 7 ◽

pp. e7022 ◽

Cited By ~ 4

Author(s):

James William ◽

Peter John ◽

Muhammad Waseem Mumtaz ◽

Ayoub Rashid Ch ◽

Ahmad Adnan ◽

...

Keyword(s):

Diabetes Mellitus ◽

Antioxidant Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Leaf Extracts ◽

Synthetic Drugs ◽

Promising Source ◽

Phenolic And Flavonoid ◽

Tof Ms

Background Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress is well established in DMT-2 pathogenesis. The synthetic drugs for DMT-2 are associated with serious side complications. Antioxidant and α-glucosidase inhibitory actions of phytochemicals from various plant species are considered as an alternative to synthetic drugs for DMT-2 management. The present study aimed to evaluate the antioxidant activity, α-glucosidase inhibitory potential and phytochemical profiling of Hyophorbe lagenicaulis. Methods The total phenolic and flavonoid contents, in vitro antioxidant activity (α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum method) and α-glucosidase inhibition of ultrasonicated hydroethanolic H. lagenicaulis leaf extracts were determined spectrophotometrically. The results of DPPH assay and α-glucosidase inhibition were reported in terms of IC50 value. The phytochemical profiling was accomplished by UHPLC-Q-TOF/MS/MS technique. Results and Discussion Findings leaped 60% ethanolic extract as rich fraction regarding total phenolic and flavonoid contents. The 60% ethanolic fraction was a promising source of natural antioxidants and α-glucosidase inhibitory agents as indicated by anti-radical and enzyme inibitory activities. Kaempferol, rutin, hesperetin 5-O-glucoside, kaempferol-coumaroyl-glucoside, luteolin 3-glucoside, Isorhamnetin-3-O-rutinoside, trimethoxyflavone derivatives and citric acid were identified by UHPLC-Q-TOF-MS/MS. These compounds were believed to be responsible for the strong antioxidant and enzyme inhibitory activity of plant extracts. The extensive metabolite profiling of H. lagenicaulis was carried out the first time as never reported previously. The H. lagenicaulis might be an appropriate choice to manage diabetes mellitus in an alternate way. The findings may be further exploited extensively for toxicity evaluation to proceed with functional food development having antidiabetic attributes.

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