scholarly journals Verification and Establishment of Hydrangea macrophylla ‘Kardinal’ x H. paniculata ‘Brussels Lace’ Interspecific Hybrids

2001 ◽  
Vol 19 (2) ◽  
pp. 85-88 ◽  
Author(s):  
S. M. Reed ◽  
G. L. Riedel ◽  
M. R. Pooler
Keyword(s):  
Rapd Markers ◽  
Leaf Shape ◽  
Leaf Length ◽  
Average Height ◽  
Hydrangea Macrophylla ◽  
Cold Hardy ◽  
Slow Growing ◽  
Putative Hybrids

Abstract An interspecific hydrangea breeding project with the goal of producing cold-hardy hydrangeas with brightly colored flowers was initiated in 1997. The objective of the current study was to transfer Hydrangea macrophylla x H. paniculata plants obtained using ovule culture to in vivo conditions and to verify their hybrid nature. Putative hybrids, representing five H. macrophylla x H. paniculata cultivar combinations, were propagated and rooted in vitro. ‘Kardinal’ x ‘Brussels Lace’ putative hybrids were the only plants that produced roots and survived transfer to the greenhouse. RAPD markers were used to verify hybridity in 13 of these plants, only 5 of which survived. Four of the ‘Kardinal’ x ‘Brussels Lace’ hybrids were greatly reduced in size and slow-growing, having an average height of only 6.4 cm (2.5 in) 8 months after being removed from in vitro conditions. Height, internode length, leaf length and leaf width were approximately six times greater in the remaining ‘Kardinal’ x ‘Brussels Lace’ hybrid than in the four small hybrids. All hybrids resembled H. paniculata in leaf shape and pubescence, and appeared to be less susceptible than H. macrophylla to powdery mildew. Intercrosses between hybrids and backcrosses to parental species will be made when the hybrids flower.

scholarly journals Biofilm formation in the lung contributes to virulence and drug tolerance of Mycobacterium tuberculosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Poushali Chakraborty ◽  
Sapna Bajeli ◽  
Deepak Kaushal ◽  
Bishan Dass Radotra ◽  
Ashwani Kumar
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Immune System ◽  
Biofilm Formation ◽  
Antimycobacterial Activity ◽  
Drug Tolerance ◽  
Host Immune System ◽  
Tissue Sections ◽  
Slow Growing

AbstractTuberculosis is a chronic disease that displays several features commonly associated with biofilm-associated infections: immune system evasion, antibiotic treatment failures, and recurrence of infection. However, although Mycobacterium tuberculosis (Mtb) can form cellulose-containing biofilms in vitro, it remains unclear whether biofilms are formed during infection in vivo. Here, we demonstrate the formation of Mtb biofilms in animal models of infection and in patients, and that biofilm formation can contribute to drug tolerance. First, we show that cellulose is also a structural component of the extracellular matrix of in vitro biofilms of fast and slow-growing nontuberculous mycobacteria. Then, we use cellulose as a biomarker to detect Mtb biofilms in the lungs of experimentally infected mice and non-human primates, as well as in lung tissue sections obtained from patients with tuberculosis. Mtb strains defective in biofilm formation are attenuated for survival in mice, suggesting that biofilms protect bacilli from the host immune system. Furthermore, the administration of nebulized cellulase enhances the antimycobacterial activity of isoniazid and rifampicin in infected mice, supporting a role for biofilms in phenotypic drug tolerance. Our findings thus indicate that Mtb biofilms are relevant to human tuberculosis.

scholarly journals Super Cooling Point Phenotypes and Cold Resistance in Hyles euphorbiae Hawk Moths from Different Climate Zones

Diversity ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 207
Author(s):  
Hana Daneck ◽  
Matthias Benjamin Barth ◽  
Martin Geck ◽  
Anna K. Hundsdoerfer
Keyword(s):  
Environmental Conditions ◽  
Cold Hardiness ◽  
Cold Treatment ◽  
Underlying Mechanism ◽  
Frost Tolerance ◽  
Cold Sensitivity ◽  
Cold Hardy ◽  
Hyles Euphorbiae

The spurge hawkmoth Hyles euphorbiae L. (Sphingidae) comprises a remarkable species complex with still not fully resolved taxonomy. Its extensive natural distribution range covers diverse climatic zones. This predestinates particular populations to cope with different local seasonally unfavorable environmental conditions. The ability of the pupae to overcome outer frosty conditions is well known. However, the differences between two main ecotypes (‘euphorbiae’ and ‘tithymali’) in terms of the inherent degree of frost tolerance, its corresponding survival strategy, and underlying mechanism have not been studied in detail so far. The main aim of our study was to test the phenotypic exhibition of pupae (as the relevant life cycle stadia to outlast unfavorable conditions) in response to combined effects of exogenous stimuli, such as daylight length and cooling regime. Namely, we tested the turnout of subitan (with fast development, unadapted to unfavorable conditions) or diapause (paused development, adapted to unfavorable external influences and increased resistance) pupae under different conditions, as well as their mortality, and we measured the super cooling point (SCP) of whole pupae (in vivo) and pupal hemolymph (in vitro) as phenotypic indicators of cold acclimation. Our results show higher cold sensitivity in ‘tithymali’ populations, exhibiting rather opportunistic and short-termed cold hardiness, while ‘euphorbiae’ produces a phenotype of seasonal cold-hardy diapause pupae under a combined effect of short daylight length and continuous cold treatment. Further differences include the variability in duration and mortality of diapause pupae. This suggests different pre-adaptations to seasonal environmental conditions in each ecotype and may indicate a state of incipient speciation within the H. euphorbiae complex.

Factors contributing to seasonal increases in inoculative freezing resistance in overwintering fire-colored beetle larvae dendroides canadensis

1998 ◽  
Vol 201 (10) ◽  
pp. 1585-1594 ◽  
Author(s):  
T Olsen ◽  
S Sass ◽  
N Li ◽  
J Duman
Keyword(s):  
Epicuticular Waxes ◽  
Freezing Resistance ◽  
Dendroides Canadensis ◽  
Ice Nucleators ◽  
Inoculative Freezing ◽  
Cold Hardy ◽  
Chamber Studies ◽  
Epicuticular Lipids

The insects and microarthropods that vary seasonally in susceptibility to cross-cuticular inoculation by external ice (inoculative freezing) represent a phylogenetically diverse group; however, few studies have explored possible mechanisms experimentally. This study documents seasonally variable inoculative freezing resistance in Dendroides canadensis beetle larvae and combines immunofluorescence, in vivo removal of epicuticular lipids and in vitro chamber studies to explore the roles of seasonal modification in the cuticle and in epidermal and hemolymph antifreeze proteins (AFPs). Seasonal cuticular modifications contribute to the inhibition of inoculative freezing since more cold-hardy larvae froze inoculatively when epicuticular waxes were removed with hexane and, in in vitro chamber experiments, cuticle patches (with the underlying epidermis removed) from winter larvae provided greater protection from inoculative freezing than did cuticle patches from summer larvae. The results indicate that seasonal modifications in epidermal and hemolymph AFPs contribute most strongly to the inhibition of inoculative freezing. Subcuticular epidermal AFPs were present in immunocytochemically labeled transverse sections of winter larvae but were absent in summer ones. Winter integument patches (cuticle with epidermis) were more resistant to inoculative freezing than were summer integument patches. Integument patches resisted inoculative freezing as well as live winter-collected larvae only when hemolymph AFP was added. The results also suggest that some integumentary ice nucleators are removed in cold-hardy larvae and that AFP promotes supercooling by inhibiting the activity of these nucleators.

Production of tumours and roots by Ephedra following Agrobacterium rhizogenes infection

1994 ◽  
Vol 72 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Niamh A. O'Dowd ◽  
David H. S. Richardson
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Agrobacterium Rhizogenes ◽  
Dry Weight ◽  
Wild Type ◽  
Bacterial Strains ◽  
Heat Treated ◽  
Slow Growing

This paper contains the first report that stems of the Gnetophyte Ephedra respond to infection by Agrobacterium rhizogenes by producing roots and tumours in vivo and in vitro. Of the bacterial strains employed, the wild-type Ar2629 gave the maximum response, and strain LBA9402 was also effective. In no case did heat-treated A. rhizogenes produce tumours or roots. Excised tumour tissues were cultured for more than 2 years in the absence of exogenous plant-growth regulators without any deterioration in growth rate. In vivo tumours of Ephedra fragilis and Ephedra minima contained up to 0.3% dry weight l-ephedrine, and slow-growing in vitro cultured tumours of E. fragilis contained up to 0.01% l-ephedrine, but alkaloid was not detected in faster growing isolates. Key words: Agrobacterium rhizogenes, alkaloid, Ephedra, l-ephedrine, Gnetophytes, gymnosperm, tumours.

Oxidative metabolism and glycolysis in benign brain tumors

1987 ◽  
Vol 67 (3) ◽  
pp. 336-340 ◽  
Author(s):  
Terry Lichtor ◽  
George J. Dohrmann
Keyword(s):  
Brain Tumors ◽  
Human Brain ◽  
Oxidative Metabolism ◽  
Glucose Utilization ◽  
Content Type ◽  
Human Brain Tumors ◽  
Elevated Glucose ◽  
Slow Growing

✓ Glucose utilization in vivo and hexokinase activity and mitochondrial oxygen consumption in vitro were measured in a series of human brain tumors. Several relatively slow-growing tumors appeared to have depressed electron-transport activities coupled with a compensatory elevated glucose utilization. These data suggest that a decrease in oxidative metabolism and a corresponding increase in glycolysis are not necessarily correlated with malignancy in certain human brain tumors.

scholarly journals Molecular Assessment of Remontant (Reblooming) Hydrangea macrophylla Cultivars

2003 ◽  
Vol 21 (2) ◽  
pp. 57-60
Author(s):  
Jon T. Lindstrom ◽  
Matthew C. Pelto ◽  
Michael A. Dirr
Keyword(s):  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Hydrangea Macrophylla ◽  
Molecular Assessment ◽  
Cold Hardy

Abstract Randomly amplified polymorphic DNA (RAPD) markers were used to distinguish five remontant and two cold-hardy Hydrangea macrophylla (Thunb.) Ser. taxa. Eleven primers generated sufficient polymorphisms to separate these seven cultivars into two groups. One group contained ‘Penny Mac’, ‘Dooley’ and ‘Nikko Blue’, while the remaining taxa, ‘David Ramsay’, ‘Endless Summer’, ‘Decatur Blue’ and ‘Oak Hill’ were clustered in the second group. All hydrangea cultivars examined are closely related; the most divergent cultivars exhibited 88% similarity based on RAPD markers.

scholarly journals In vivo Rooting, Acclimatization and Ex situ Conservation of Opuntia ficus-indica (L.) Mill. (Beles) in Tigray Region of Ethiopia, Africa

2019 ◽  
Vol 5 (04) ◽  
pp. 265-269
Author(s):  
Shastri Prasad Shukla ◽  
Zinabu Hailu ◽  
Yemene Kehsay
Keyword(s):  
Fruits And Vegetables ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Ornamental Plants ◽  
Ex Situ ◽  
Average Height ◽  
Opuntia Ficus Indica ◽  
Tigray Region

Opuntia ficus-indica (L.) Mill. commonly known as prickly pear or Beles is a xerophytic, succulent, CAM (Crassulacean Acid Metabolism) plant introduced in Ethiopia (Africa), particularly to Tigray region between 1848 and 1920. It is known as a multipurpose plant since it can be used as human food (fruits and vegetables), medicine and ornamental plants, fodder, natural wind barrier, soil stabilizer, re-vegetation resource in eroded soils. Stem of this plant has been reported to treat diabetes and useful in the cure of hyperlipidemy (excess of lipids in the blood) and obesity. It is well recognized for their wound healing properties and anticancer effects. Conventionally, it is propagated by seeds but physiological limitations of the seeds, such as; low germination rate, genetic segregation, less guarantee of genetic stability, a long juvenile stage, less availability and low viability, and slow seedling growth rate are major constraints in the multiplication of this species in nature. In the present study we developed an efficient procedure for mass multiplication, acclimatization and ex-situ conservation of this succulent plant in Adigrat region of Ethiopia (Africa). In the present study out of six used in vitro - raised clones, maximum average height of the plantlet (13 cm.) and width (8.5 cm.) were observed in the plantlets (X6 ) and (X5 ) clones at 30.29 and 29.15oC temperatures, respectively on a modified substrate. Here, the effects of temperature and duration were significant in the increment of clones height and width. Thus, the developed efficient acclimatization techniques of this crop will ensure the supply of the in vitro-raised plantlets throughout the year to the consumers in Tigray region of Ethiopia.

scholarly journals Surface Roughness of Commercial Composites after Different Polishing Protocols: An Analysis with Atomic Force Microscopy

2010 ◽  
Vol 4 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Luca Giacomelli ◽  
Giacomo Derchi ◽  
Andrea Frustaci ◽  
Orlando Bruno ◽  
Ugo Covani ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Colonization ◽  
Average Height ◽  
Resin Composites ◽  
Composite Surface ◽  
Force Microscopy ◽  
Atomic Force ◽  
Significant Difference

Background and Aims: Polishing may increase the surface roughness of composites, with a possible effect on bacterial growth and material properties. This preliminary in vitro study evaluates the effect of three different polishing systems (PoGo polishers, Enhance, Venus Supra) on six direct resin composites (Gradia Direct, Venus, Venus Diamond, Enamel Plus HFO, Tetric Evoceram, Filtek Supreme XT). Materials and Methods: For each composite, 12 square specimens were prepared: 9 specimens were polished, three for each different method, while three specimens were used as controls. Surface roughness was determined with AFM by measuring Root Mean Square (RMS) of average height. Results: PoGo polisher determined a significantly rougher surface, versus controls, in 5 out of 6 composites evaluated. Some significant differences from unpolished controls were observed also for Enhance polishing. Polishing with Venus Supra did not result in any significant difference in surface roughness versus controls. No differences were observed between different polishing systems. Conclusions: These preliminary results suggest that Venus Supra polishing system could determine a smoother composite surface if compared to the other polishing systems tested. On this basis, we are conducting an in vivo study to evaluate bacterial colonization on some combinations of composites and polishing protocols.

Studies on the in vitro formation of periostracum in Helisoma duryi: the influence of the dorsal epithelium on calcium incorporation

1984 ◽  
Vol 62 (6) ◽  
pp. 1177-1180 ◽  
Author(s):  
S. C. Kunigelis ◽  
A. S. M. Saleuddin
Keyword(s):  
Epithelial Tissue ◽  
Whole Brain ◽  
Fast Growing ◽  
Cerebral Ganglia ◽  
Helisoma Duryi ◽  
Shell Deposition ◽  
Slow Growing

Mantle collar tissue was found to produce periostracum when placed in vitro. The rate of shell deposition in vivo was reflected in the in vitro rate of periostracum formation. The addition of whole brain from fast-growing donors to mantle collar from slow-growing animals was found to increase the amount of periostracum produced in vitro. This effect was further enhanced by removing the cerebral ganglia lateral lobes prior to incubation. The presence of dorsal epithelial tissue was found to increase the incorporation of calcium into periostracum formed in vitro.

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