scholarly journals Prevalence, Distribution and Identification of Phytophthora Species from Bleeding Canker on European Beech

2010 ◽  
Vol 28 (3) ◽  
pp. 150-158
Author(s):  
Angela H. Nelson ◽  
Jerry E. Weiland ◽  
George W. Hudler
Keyword(s):  
New York ◽  
Pathogen Detection ◽  
Management Strategies ◽  
European Beech ◽  
Disease Prevalence ◽  
Phytophthora Species ◽  
Phytophthora Cactorum ◽  
Building Management ◽  
Symptomatic Tissue ◽  
Soil Surveys

Abstract While bleeding canker of European beech trees (Fagus sylvatica) has long been recognized as a problem in Europe and North America, the cause in the northeastern United States has not been clear. To resolve this, we surveyed for disease prevalence on European beech, identified the pathogens involved, proved their pathogenicity, compared protocols for pathogen detection, and conducted a soil assay to determine pathogen presence in soil surrounding established trees in residential and commercial landscapes in New York, Pennsylvania, Maryland, Connecticut and Massachusetts. Approximately 40% of surveyed trees had bleeding cankers. While Phytophthora cactorum, P. gonapodyides, P. cambivora and two newly described species, P. citricola I and P. plurivora, were recovered from symptomatic tissue, P. citricola I and P. cactorum were most prevalent. All caused disease when artificially inoculated into European beech sapling stems, although P. cambivora and P. gonapodyides produced significantly smaller lesions. Recovery of the pathogen from symptomatic tissue using selective media, the preferred method of diagnosis, was significantly higher in the fall. ELISA detection was more successful and worked regardless of season, but did not allow identification to the species level. All five Phytophthora species were found in soil surveys; P. cambivora was most common, followed by P. cactorum and P. citricola I. These results provide a foundation for building management strategies to protect valuable specimens of European beech.

scholarly journals Bleeding Canker of European Beech in Southeastern New York State: Phytophthora Species, Spatial Analysis of Disease, and Periodic Growth of Affected Trees

2014 ◽  
Vol 32 (3) ◽  
pp. 113-125 ◽  
Author(s):  
Shawn C. Kenaley ◽  
Clifford Rose ◽  
Patrick J. Sullivan ◽  
George W. Hudler
Keyword(s):  
New York ◽  
New York State ◽  
European Beech ◽  
Phytophthora Species ◽  
Point Pattern ◽  
York State ◽  
Disease Progress ◽  
Phytophthora Spp ◽  
Periodic Growth ◽  
Residual Growth

The epidemiology of bleeding canker, a Phytophthora-associated disease, on European beech remains unclear. Pathogen surveys as well as dendrological and spatial point pattern analyses (SPPA) were conducted to identify factors contributing to disease progress on beech at the Green-Wood Cemetery (GWC) and Planting Fields Arboretum (PFA) in southeastern New York State. Phytophthora pini was the predominant Phytophthora isolated from cankers as well as soil under asymptomatic and diseased (canker bearing) European beech at each site. No significant differences existed between asymptomatic and Phytophthora-infected trees according to diameter breast height, elevation, and the Phytophthora spp. The radial growth (25-yr chronology for 1986 to 2010) of infected European beech at GWC and PFA, however, was significantly less when compared to asymptomatic beech; yet, residual growth was similar among the latter cohorts, providing no evidence for the instigatory effect(s) of environmental stressors on disease progress. SPPA demonstrated all beech at GWC and PFA were planted in non-random aggregates, whereas the distribution of diseased European beech did not deviate from random. Collectively, results indicated bleeding canker is a slow, chronic disease and the overland tree-to-tree spread of P. pini, and accompanying Phytophthora spp., is rare or does not occur at GWC or PFA.

scholarly journals Aggressiveness of Phytophthora cactorum, P. citricola I, and P. plurivora from European Beech

Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1009-1014 ◽  
Author(s):  
Jerry E. Weiland ◽  
Angela H. Nelson ◽  
George W. Hudler
Keyword(s):  
New York ◽  
Sugar Maple ◽  
Disease Incidence ◽  
European Beech ◽  
Infested Soil ◽  
Phytophthora Cactorum ◽  
Canker Disease ◽  
Common Lilac ◽  
Oyster Bay ◽  
Leaf Disks

Phytophthora cactorum, P. citricola I, and P. plurivora cause bleeding cankers on mature European beech (Fagus sylvatica) trees in the northeastern United States. Inoculation experiments were conducted to compare the aggressiveness of the three Phytophthora spp. on stems, leaf disks, and roots of European beech and common lilac (Syringa vulgaris) seedlings. Isolates were obtained from bleeding cankers on European beech from five cities in New York (Albany, Ithaca, Oyster Bay, Plainview, and Rochester) and from a bleeding canker on sugar maple in Ithaca, NY. Stems were inoculated with colonized agar plugs, leaf disks with a zoospore suspension, and roots via infested soil at three inoculum levels. All organs of inoculated beech and lilac developed disease except for lilac roots inoculated with zoospores of P. cactorum. Disease incidence, severity, and plant survival were dependent on isolate and were also influenced by the tissue inoculated and host. Isolates of P. cactorum were the least aggressive and caused less necrosis than isolates of P. citricola I and P. plurivora. Results emphasize the utility of stem and root inoculation for evaluation of this canker disease and underscore critical differences in species aggressiveness.

scholarly journals Using excess deaths and testing statistics to determine COVID-19 mortalities

2021 ◽  
Author(s):  
Lucas Böttcher ◽  
Maria R. D’Orsogna ◽  
Tom Chou
Keyword(s):  
New York ◽  
New York City ◽  
Disease Prevalence ◽  
Statistical Testing ◽  
Biased Sampling ◽  
Mortality Data ◽  
Sir Epidemic Model ◽  
Excess Death ◽  
Sir Epidemic ◽  
Excess Deaths

AbstractFactors such as varied definitions of mortality, uncertainty in disease prevalence, and biased sampling complicate the quantification of fatality during an epidemic. Regardless of the employed fatality measure, the infected population and the number of infection-caused deaths need to be consistently estimated for comparing mortality across regions. We combine historical and current mortality data, a statistical testing model, and an SIR epidemic model, to improve estimation of mortality. We find that the average excess death across the entire US from January 2020 until February 2021 is 9$$\%$$ % higher than the number of reported COVID-19 deaths. In some areas, such as New York City, the number of weekly deaths is about eight times higher than in previous years. Other countries such as Peru, Ecuador, Mexico, and Spain exhibit excess deaths significantly higher than their reported COVID-19 deaths. Conversely, we find statistically insignificant or even negative excess deaths for at least most of 2020 in places such as Germany, Denmark, and Norway.

scholarly journals Controlling a Multicenter Outbreak Involving the New York/Japan Methicillin-ResistantStaphylococcus aureusClone

2007 ◽  
Vol 28 (7) ◽  
pp. 845-852 ◽  
Author(s):  
G. W. Coombs ◽  
H. Van Gessel ◽  
J. C. Pearson ◽  
M.-R. Godsell ◽  
F. G. O'Brien ◽  
...  
Keyword(s):  
Public Health ◽  
New York ◽  
Molecular Typing ◽  
Management Strategies ◽  
Research Unit ◽  
Management Policy ◽  
Teaching Hospitals ◽  
Term Care ◽  
Outbreak Management ◽  
Healthcare Facilities

Objective.To describe the control of an outbreak of infection and colonization with the New York/Japan methicillin-resistantStaphylococcus aureus(MRSA) clone in multiple healthcare facilities, and to demonstrate the importance of making an MRSA management policy involving molecular typing of MRSA into a statewide public health responsibility.Setting.A range of healthcare facilities, including 2 metropolitan teaching hospitals and a regional hospital, as well as several community hospitals and long-term care facilities in a nonmetropolitan healthcare region.Interventions.A comprehensive, statewide MRSA epidemiological investigation and management policy.Results.In May 2005, there were 3 isolates referred to the Western Australian Gram-Positive Bacteria Typing and Research Unit that were identified as the New York/Japan MRSA clone, a pandemic MRSA clone with the ability to spread and replace existing clones in a region. Subsequent investigation identified 28 additional cases of infection and/or colonization dating from 2002 onward, including 1 involving a colonized healthcare worker (HCW) who had previously been hospitalized overseas. Of the 31 isolates detected, 25 were linked epidemiologically and via molecular typing to the isolate recovered from the colonized HCW. Four isolates appeared to have been introduced separately from overseas. Although the isolate from the single remaining case patient was genetically indistinct from the isolates that spread within Western Australia, no specific epidemiological link could be established. The application of standard outbreak management strategies reduced further spread.Conclusions.The elimination of the New/York Japan MRSA clone in a healthcare region demonstrates the importance of incorporating MRSA management policy into statewide public health programs. The mainstays of such programs should include a comprehensive and effective outbreak identification and management policy (including pre-employment screening of HCWs, where applicable) and MRSA clone identification by multilocus sequence typing.

Detection of oomycide-insensitive Phytophthora isolates in Maryland ornamental nurseries and Mid-Atlantic landscapes provide data for reconsidering management strategies

2021 ◽  
Author(s):  
Justine Beaulieu ◽  
Johanna Del Castillo Munera ◽  
Yilmaz Balci
Keyword(s):  
Growth Rate ◽  
Mycelial Growth ◽  
Management Strategies ◽  
Resistance Management ◽  
Phytophthora Species ◽  
Oak Forests ◽  
Relative Growth ◽  
Off Label ◽  
Potting Media ◽  
Almost All

Five Phytophthora species comprising a total of 243 isolates (77 P. cinnamomi, 23 P. citrophthora, 18 P. multivora, 18 P. pini, and 107 P. plurivora) were screened for sensitivity to mefenoxam, fosetyl-Al, dimethomorph, dimethomorph + ametoctradin and fluoxastrobin using amended agar assays. Mefenoxam-insensitive isolates were detected within P. cinnamomi (4%), P. multivora (11%), and P. plurivora (12%) even at approximately 2.5x the recommended label rate. These isolates were also insensitive to higher (off-label) concentrations of fluoxastrobin. Concentrations of dimethomorph (400 g/mL) and dimethomorph + ametoctradin (100 g/mL) were mostly effective in mycelial growth inhibition, but two P. plurivora isolates were insensitive, suggesting that resistance management is required. All mefenoxam-insensitive isolates were sensitive to fosetyl-Al at the label rate. Surprisingly, the populations of P. cinnamomi from mid-Atlantic oak forests included insensitive isolates. With almost all species, isolates recovered from asymptomatic hosts (e.g., soil/potting media collected of randomly selected asymptomatic hosts) had a significantly greater relative growth rate when compared to isolates recovered from symptomatic hosts (e.g., isolates recovered from lesions or wilted plants). These findings suggest that mefenoxam should no longer be used to manage oomycetes in Maryland ornamental nurseries and that the use of fluoxastrobin should be limited.

scholarly journals PhytophthoraSpecies Are Common on Nursery Stock Grown for Restoration and Revegetation Purposes in California

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 448-455 ◽  
Author(s):  
S. Rooney-Latham ◽  
C. L. Blomquist ◽  
K. L. Kosta ◽  
Y. Y. Gou ◽  
P. W. Woods
Keyword(s):  
North America ◽  
Phytophthora Species ◽  
Molecular Techniques ◽  
Crown Rot ◽  
Native Plant ◽  
Nursery Stock ◽  
Plant Nursery ◽  
Root And Crown Rot ◽  
Symptomatic Tissue ◽  
First Time

Phytophthora tentaculata was detected for the first time in North America in 2012 in a nursery on sticky monkeyflower plant (Diplacus aurantiacus) and again in 2014 on outplanted native plants. At that time, this species was listed as a federally actionable and reportable pathogen by the USDA. As a result of these detections, California native plant nurseries were surveyed to determine the prevalence of Phytophthora species on native plant nursery stock. A total of 402 samples were collected from 26 different native plant nurseries in California between 2014 and 2016. Sampling focused on plants with symptoms of root and crown rot. Symptomatic tissue was collected and tested by immunoassay, culture, and molecular techniques (PCR). Identifications were made using sequences from the internal transcribed spacer (ITS) rDNA region, a portion of the trnM-trnP-trnM, or the atp9-nad9 mitochondrial regions. Phytophthora was confirmed from 149 of the 402 samples (37%), and from plants in 22 different host families. P. tentaculata was the most frequently detected species in our survey, followed by P. cactorum and members of the P. cryptogea complex. Other species include P. cambivora, P. cinnamomi, P. citricola, P. hedraiandra, P. megasperma, P. multivora, P. nicotianae, P. niederhauserii, P. parvispora, P. pini, P. plurivora, and P. riparia. A few Phytophthora sequences generated from mitochondrial regions could not be assigned to a species. Although this survey was limited to a relatively small number of California native plant nurseries, Phytophthora species were detected from three quarters of them (77%). In addition to sticky monkeyflower, P. tentaculata was detected from seven other hosts, expanding the number of associated hosts. During this survey, P. parvispora was detected for the first time in North America from symptomatic crowns and roots of the nonnative Mexican orange blossom (Choisya ternata). Pathogenicity of P. parvispora and P. nicotianae was confirmed on this host. These findings document the widespread occurrence of Phytophthora spp. in native plant nurseries and highlight the potential risks associated with outplanting infested nursery-grown stock into residential gardens and wildlands.

scholarly journals A New Phytophthora sp. Causing a Basal Canker on Beech in Italy

Plant Disease ◽  
2003 ◽  
Vol 87 (8) ◽  
pp. 1005-1005 ◽  
Author(s):  
E. Motta ◽  
T. Annesi ◽  
A. Pane ◽  
D. E. L. Cooke ◽  
S. O. Cacciola
Keyword(s):  
New Species ◽  
Central Italy ◽  
European Beech ◽  
Phytophthora Species ◽  
Deciduous Tree ◽  
Reference Isolate ◽  
European Chestnut ◽  
Pure Cultures ◽  
Basal Portion ◽  
A New Species

In autumn 2001, bleeding cankers were observed on the basal portion of the trunk of a declining tree in a forest stand of European beech (Fagus sylvatica L.) in Latium (central Italy). A Phytophthora sp. was isolated consistently from infected trunk bark using whole apples as bait. Isolations were made from brown lesions that developed in the apple pulp around the inserted bark pieces. Pure cultures were obtained by using hyphal tip transfers. Colonies were stellate on V8 juice agar (V8A), uniform to slightly radiate on cornmeal agar, and cottony, without a distinct growth pattern on potato dextrose agar (PDA). On V8A, radial growth rates were 2.1, 4.8, and 4.5 mm/day at 10, 15, and 20°C, respectively. Colonies grew slowly at 5 and 25°C, but failed to grow at 30°C. On PDA, growth was 1.7 and 1.4 mm/day at 15 and 20°C, respectively. Catenulate hyphal swellings formed on solid and liquid media. Sporangia formed abundantly at 15°C, were ovoid to obpyriform, semipapillate, occasionally bipapillate, and had narrow exit pores (mean diameter = 5.4 μm). On V8A, pores were 40 to 50 μm in length and 25 to 40 μm in breadth. Isolates were homothallic with paragynous antheridia, oogonia were spherical with diameters from 32 to 35 μm, and oospores were plerotic with diameters from 20 to 30 μm. Electrophoretic banding patterns of mycelial proteins and isozymes (alkaline phospatase, esterase, glucose-6-phospate dehydrogenase, malate dehydrogenase, and superoxide dismutase) of beech isolates were distinct from those of reference isolates of semipapillate Phytophthora species, including P. citricola, P. hibernalis, P. ilicis (IMI 158964), P. psychrophila (CBS 803.95), and P. syringae from citrus fruits, whose identification had been confirmed on the basis of internal transcribed spacer (ITS)-restriction fragment length polymorphism (RFLP) patterns and sequences. Conversely, the electrophoretic phenotype and the ITS-RFLP pattern (and sequence) of the beech isolates were identical to those of a reference isolate (Ph24) from Quercus cerris, which was originally identified as P. syringae on the basis of morphological and cultural characters (1). However, the isolate Ph24 has been reexamined, and morphological and cultural characteristics as well as the ITS sequence would indicate that this isolate is a new species not yet formally described, for which the name P. pseudosyringae has been suggested (2). The pathogenicity of a beech isolate (IMI 390500) was compared to that of an Italian P. cambivora isolate from European chestnut by inoculating the stems of 16-month-old beech seedlings (10 replicates), which were placed at 18°C with a 12-h photoperiod. The beech isolate produced lesions averaging 2 cm long after 2 months, while those produced by the P. cambivora isolate averaged 3 cm. Control seedlings inoculated with sterile agar did not develop symptoms. The pathogen was reisolated from lesions to fulfil Koch's postulates. To our knowledge, this is the first report of this new Phytophthora sp. on beech in Italy. Conversely, the same species has been reported to be associated with decline of oak stands (1). References: (1) G. P. Barzanti et al. Phytopathol. Mediterr. 40:149, 2001. (2) T. Jung et al. Phytophthora pseudosyringae sp. nov., a new species causing root and collar rot of deciduous tree species in Europe. Mycol. Res. (In press).

scholarly journals Challenges and Prospects for Building Resilient Disease Management Strategies and Tactics for the New York Table Beet Industry

Agronomy ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 112 ◽  
Author(s):  
Sarah Pethybridge ◽  
Julie Kikkert ◽  
Linda Hanson ◽  
Scot Nelson
Keyword(s):  
New York ◽  
Disease Management ◽  
Management Strategies
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