scholarly journals THE USE OF NANO-SILVER FOR DISINFECTION OF Pennisetum alopecuroides PLANT MATERIAL FOR TISSUE CULTURE

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Marzena Parzymies ◽  
Krystyna Pudelska ◽  
Monika Poniewozik
Keyword(s):  
Tissue Culture ◽  
Plant Material ◽  
Nodal Explants ◽  
Grass Species ◽  
Silver Particles ◽  
Ag Nps ◽  
Nano Silver ◽  
The Media ◽  
Pennisetum Alopecuroides

Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm Ag–3 NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

SYNTHESIS OF DNA AND LECITHIN IN TISSUE CULTURE AND OESTROGEN RECEPTOR ACTIVITY IN RAT MAMMARY TUMOURS DEPENDENT ON AND INDEPENDENT OF THE OVARY

1979 ◽  
Vol 81 (2) ◽  
pp. 183-198 ◽  
Author(s):  
ANNE-MARIE SCOTT ◽  
SUSAN MURPHY ◽  
R. A. HAWKINS
Keyword(s):  
Tissue Culture ◽  
Dna Synthesis ◽  
Oestrogen Receptor ◽  
Receptor Activity ◽  
Sprague Dawley ◽  
Mammary Tumours ◽  
Sprague Dawley Rats ◽  
The Media

Dimethylbenz(a)anthracene (DMBA)-induced and transplanted rat mammary tumours (2 lines) were examined for oestrogen receptor activity, and for sensitivity to hormones in vivo (by ovariectomy) and in vitro (by tissue culture). In vivo, the growth of all tumours induced by the administration of DMBA in random-bred Sprague–Dawley rats was found to be dependent on the ovary, whilst in all transplanted tumours (12 TG-3 and six TG-5 lines), maintained in an inbred strain of Sprague–Dawley rats, growth was found to be independent of the ovary. In vitro, the capacity for DNA synthesis in DMBA-induced tumours was better maintained after 24 h when insulin (10 μg/ml) and corticosterone (5 μg/ml) or insulin, corticosterone and prolactin (each 5 μg/ml) were present in the medium (five out of 12 and eight out of 11 tumours respectively); no effect of hormones in the media was detected after 48 h. In the transplanted tumours, no effect of hormones on DNA synthesis was detected after either 24 or 48 h of culture. Synthesis of lecithin was not detectably influenced by the presence of hormones in either DMBA-induced or transplanted tumours. Oestrogen receptor concentrations were, on average, significantly higher in the DMBA-induced tumours than in either line of transplanted tumour. For 22 DMBA-induced tumours and 15 transplanted tumours, the effect of hormones in vitro (`response') was directly correlated with receptor concentration at time 0 (Spearman's ρ = + 0·59) and inversely correlated with the rate of DNA synthesis (`basal') at time 0 (Spearman's ρ = −0·62). No single parameter or pair of parameters permitted accurate distinction between the tumour types.

Isozyme assessment of the genetic stability of micropropagated hybrid larch (Larix × eurolepis Henry)

1995 ◽  
Vol 25 (7) ◽  
pp. 1103-1112 ◽  
Author(s):  
Sylvie Richard ◽  
Sylvie Gauthier ◽  
Sylvie Laliberté
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Genetic Stability ◽  
Physiological Response ◽  
Culture Conditions ◽  
Hybrid Larch ◽  
Short Shoot ◽  
In Vitro Shoots ◽  
The Media

The search for the occurrence of somaclonal variation of in vitro shoots and acclimatized plants of a hybrid larch (Larix × urolepis Henry) clone was performed by the analysis of eight isozyme systems. Cultures were established from short shoot buds of mature material. The effects of growth regulators in the media, subculture intervals, and periods in culture were analyzed for in vitro shoots. Variability was found in in vitro shoots but appeared to be related to a physiological response to culture conditions. Once acclimatized, most tissuecultured plants expressed the same enzymatic patterns as those of control plants (stecklings and the ortet). The variations observed for some acclimatized plants were also observed in control plants and were not related to ontogenic stage. Results from the isoenzymatic systems studied showed that hybrid larch plants regenerated from tissue culture were not significantly different from stecklings and the ortet.

Antibacterial and Nontoxic Nano Silver PLLA Composites for Tissue Engineering

2008 ◽  
Vol 47-50 ◽  
pp. 849-852 ◽  
Author(s):  
Lin Li ◽  
Yi Li ◽  
Jia Shen Li ◽  
Lei Yao ◽  
Arthur F.T. Mak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Antibacterial Property ◽  
In Vitro Cytotoxicity ◽  
Silver Particles ◽  
Nano Silver

Poly-L-Lactide (PLLA) composites were prepared with nano silver particles with the weight ratios of nano silver particles to PLLA (Ag/PLLA) at 0.5%, 2.5%, 5%, 7.5% and 10% (w/w). In vitro cytotoxicity tests were conducted firstly to evaluate the cytotoxicity of the composites. The results indicated that PLLA, 0.5%, 2.5% and 5% Ag/PLLA composites were nontoxic to cells, while 7.5% and 10% Ag/PLLA composites were significantly toxic to cells. Cell proliferation experiments and antibacterial tests were also performed. 5% Ag/PLLA was found the best for cell proliferation with obvious antibacterial property. Thus, Ag/PLLA can be used as nontoxic scaffolds for tissue engineering with antibacterial property.

scholarly journals Kajian Penambahan BahanOrganik Pada Media Tanam VW Pada Organogenesis AnggrekDendrobium SecaraIn Vitro

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Siti Rahmah ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Tissue Culture ◽  
Organic Matter ◽  
Optimization Technique ◽  
Media Composition ◽  
Growth Optimization ◽  
The World ◽  
The Media ◽  
Number Of Leaves ◽  
Bean Sprouts

In vitro tissue culture is a growth optimization technique of Dendrobium orchid with according to media composition. Nutritions in the media are important for dendrobium orchid. Dendrobium orchid  include plant from orchidaceae family its spread throughout the world like indonesia. Its features are easily planted, intersest is continuous and varied, easily assembled, the flower crown is not easy to fall and wither. Research aimed at obtaining media compositions that are easily available and able to fulfill the needs of orchid plants. The research was conducted using descriptive methods to compare different trearment; Vacin & Went and VW media with adding organic matter; extract bean sprouts, potato extrac, and water coconut; wich is conducted for eight weeks after planting. The result of addition organic matter on VW media was different toward organogenesis of orchid. The average number of shoots is 1.8; the number of leaves average of 6.8 and the number of roots average of 3.6 formed from two until eight weeks after culture.Keywords: tissue culture, growing media, Dendrobium orchid, organogenesis.ABSTRAKKultur jaringan in vitro adalah salah satu teknik optimalisasi pada pertumbuhan tanaman angrek Dendrobium dengan menyesuaikan komposisi medianya. Nutrisi yang terdapat di dalam media sangat penting bagi pertumbuhan anggrek. Anggrek Dendrobium termasuk tanaman dari keluarga Orchidaceae yang penyebarannya sampai ke pelosok dunia seperti Indonesia. Keistimewaanya mudah ditanam, bunganya terus-menerus dan bermacam-macam, mudah disusun, serta mahkota bunga tidak mudah jatuh dan layu. Penelitian yang bertujuan untuk mendapatkan komposisi media yang mudah didapat dan mampu memenuhi kebutuhan tanaman anggrek. Penelitian dilakukan menggunakan metode deskriptif untuk membandingkan perlakuan media yang berbeda yaitu media Vacin & Went, dan VW dengan penambahan bahan organik; ekstrak tauge kacang hijau, ekstrak kentang, dan air kelapa muda; yang dilakukan selama delapan minggu setelah tanam. Hasil penambahan bahan organik pada media VW berbeda terhadap organogenesis eksplan anggrek. Jumlah tunas rata-rata 1,8; Jumlah daun rata-rata 6,8 dan jumlah akar rata-rata 3,6 yang terbentuk dari dua minggu setelah kultur (MSK) sampai minggu terakhir pengamatan delapan MSK.Kata kunci: kultur jaringan, media tanam, angrek Dendrobium,organogenesis.

scholarly journals PENGARUH PEMANASAN DAN ASAL EKSPLAN PADA PERTUMBUHAN DAN PERKEMBANGAN BAWANG MERAH (Allium ascolonicum L.)

Agrin ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 10
Author(s):  
Asih K. Karjadi ◽  
Neni Gunaeni
Keyword(s):  
Tissue Culture ◽  
Growth And Development ◽  
Shoot Tip ◽  
Meristematic Tissue ◽  
Culture Techniques ◽  
Heating Treatment ◽  
The Media ◽  
Treatment Research ◽  
Bacteria And Fungi

Tanaman bawang merah (Allium ascolonicum L) termasuk dalam genus Allium sp yang diperbanyak secaravegetatif melalui umbi. Perbanyakan benih bawang sudah dilakukan secara in vitro (konvensional), untuk tujuanpeningkatan mutu atau hanya perbanyakan tanaman . Penelitian dilaksanakan di Laboratorium Kultur JaringanBalai Penelitian Tanaman Sayuran, pada bulan Februari sampai Agustus 2014. Untuk menghasilkan tanamanbebas penyakit terutama virus dapat digunakan teknik kultur jaringan yang dikombinasikan dengan perlakuanpemanasan. Penelitian bertujuan untuk melihat pengaruh pemanasan dan sumber eksplan terhadap pertumbuhandan perkembangan eksplan bawang merah.. Perlakuan pemanasan bahan eksplan bawang merah dilakukan secarabertahap selama 4 minggu, masing-masing 1 minggu untuk suhu (30, 33, 35 dan 37 oC). Media yang dipergunakanuntuk penumbuhan eksplan adalah MS + MS vits + sucrose 30 g/l + IAA 2 mg/l + Kinetin 2 mg/l + GA3 0.01mg/l agar gelgro 2 g/l pH 5.7. Penelitian terdiri dari 2 kegiatan yaitu Perlakuan pada cv. Pikatan, dan pada cv.Bima Brebes. Sebagai eksplan dipergunakan yaitu (1/3 bulb/ umbi) dan (shoot tip/ jaringan meristematik denganbeberapa daun primordia). Perlakuan eksplan yang digunakan yaitu tanpa pemanasan dan dengan pemanasan.Pertumbuhan dan perkembangan dari planlet diamati pada penelitian ini. Hasil dari penelitian menunjukkanbahwa perlakuan pemanasan bahan eksplan bawang merah cv. Bima Brebes, belum menurunkan persentase planletyang terinfeksi virus. Eksplan (1/3 bulb) dan (shoot tip), mempunyai pertumbuhan eksplan diatas 50%. Umumnyasemakin kecil eksplan persentase planlet abnormal semakin tinggi. Kontaminasi kultur umumnya disebabkanbakteri dan jamur yang terbawa dari eksplan (endogen). Perlakuan pemanasan bahan eksplan bawang merah secaravisual tidak berpengaruh pada persentase pertumbuhan dan persentase kultur terkontaminasi.Kata kunci: Bawang merah, Allium ascolonicum L, pemanasan, asal eksplanABSTRACTRed onion plants (Allium ascolonicum L) are included in the genus Allium sp which is propagatedvegetatively through tubers. Propagation of onion seeds has been done in vitro (conventionally), for the purposeof quality improvement or only plant propagation. The research was carried out at the Tissue Culture Laboratory,Balai Penelitian Tanaman Sayuran (Balitsa) from February to August 2014. To produce plants free of mainly viraldiseases can be used tissue culture techniques combined with heating treatment. Research aims were to look atthe effect of heating and explants on the growth and development of red onion explants. The treatment of the onionexplants was heated gradually for 4 weeks, 1 week each for temperatures (30, 33, 35 and 37 oC). The media usedfor the growth of explants is MS + MS vits + sucrose 30 g / l + IAA 2 mg / l + Kinetin 2 mg / l + GA3 0.01 mg / lso that gelgro 2 g / l pH 5.7. The study consisted of 2 activities, namely, treatment at cv. Pikatan, and at cv. BimaBrebes. As explants it is used (1/3 bulb / tuber) and (shoot tip / meristematic tissue with several leaves ofprimordia). The explants treatment used are without heating and by heating. Growth and development of plantletswas observed in this study. The results showed that the heating treatment of explants onions cv. Bima Brebes, hasnot reduced the percentage of plantlets infected with the virus. Explants (1/3 bulb) and (Shoot tip), have explantsgrowth above 50%. Generally the smaller explants the higher percentage of abnormal plantlets. Culturecontamination is generally caused by bacteria and fungi that are carried away from explants (endogenous). Thetreatment of heating the onion explants material visually had no effect on the percentage of growth and percentageof contaminated culture.Keywords: red onion, Allium ascolonicum L, heating treatment, source of explant

scholarly journals IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

2018 ◽  
Vol 4 (2) ◽  
pp. 60
Author(s):  
Asgar Taiyeb ◽  
Baharuddin Baharuddi
Keyword(s):  
Tissue Culture ◽  
Culture Techniques ◽  
In Vitro Multiplication ◽  
Supportive Environment ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
The Difference ◽  
Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of  Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to  in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

scholarly journals The effect of media and growth regulators on in vitro rooting of olive (Olea europaea L.) cv. Gordal.

2007 ◽  
Vol 1 (1) ◽  
pp. 5-15
Author(s):  
Qais Jamel Abdul Majeed
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
In Vitro Rooting ◽  
Nodal Explants ◽  
Growth Substances ◽  
Surface Sterilization ◽  
Rooting Media ◽  
Half Strength ◽  
The Media

Nodal explants of difficult-to-root Gordal olive were cultured on half-strength OM medium after surface sterilization with 15% NaOCl for 20 minutes. The in vitro-proliferated shoots were then submitted for 25 days to rooting trials including various concentrations of NAA and IBA and different half-strength rooting media as well.Among the media tested during rooting stage, highest rooting percentage 62% found in both OM and MS half-strength media when both the growth substances were used, and 87.5 and 90% gained when IBA and NAA were added to the rooting media at 2 Mg/L each, respectively.Highest number of roots 1.85 and highest root length 3.09 mm obtained with half-strength MS and OM media respectively, when IBA was used with these media. And IBA at 2 Mg/L in the rooting media gave the highest number of roots 2.5 with 4.13 mm length.However, 2 Mg/L NAAin the rooting media gave 1.94 number of roots with length of 3.88 mm, while number of roots 1.38 with 2.79 length obtained with half- strength OM media when NAA was used in the rooting media .It is therefore concluded that best medium for in vitro rooting of Gordal microcuttings is half- strength OM supplemented with 2 mg/L of IBA.

scholarly journals Novel Laboratory Exercises in Plant Tissue Culture: In Vitro Asymbiotic Germination of Orchid Seeds

1994 ◽  
Vol 4 (3) ◽  
pp. 315-317 ◽  
Author(s):  
Kenneth W. Mudge ◽  
Chin-Chang Chu
Keyword(s):  
Tissue Culture ◽  
Plant Material ◽  
Stock Plant ◽  
Asymbiotic Seed Germination ◽  
Laboratory Exercise ◽  
Flower Pollination ◽  
Orchid Seed ◽  
Plant Flower ◽  
Single Laboratory

In vitro asymbiotic seed germination, subculture, and outplanting of orchids is presented as a laboratory exercise suitable for students of plant propagation or tissue culture. Dendrobium antennatum (Lindley), Phalaenopsis (Blume) white hybrid, or both, are used in this exercise because they flower predictably in the greenhouse, are reliable for seed production, and germinate and grow rapidly in vitro. The exercises can be used to instruct students in the skills involved in orchid seed sterilization, sowing, and culture, as well as instruct students in the unique features of orchid reproductive biology and symbiosis. A schedule is suggested for stock plant flower pollination, capsule harvest, seed sowing, and seedling subculture so that the necessary plant material is available for students to sow, subculture, and outplant seedlings during a single laboratory session.

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