Prevalence of vesivirus in a laboratory-based set of serum samples obtained from dairy and beef cattle

2006 ◽  
Vol 228 (2) ◽  
pp. 260-260
Author(s):  
Andreas Kurth ◽  
James F. Evermann ◽  
Douglas E. Skilling ◽  
David O. Matson ◽  
Alvin W. Smith
Keyword(s):  
Beef Cattle ◽  
Serum Samples

scholarly journals Epidemiological characteristics of Leptospira spp. infection in bovine herds in Mato Grosso do Sul, Brazil

2021 ◽  
Vol 58 ◽  
pp. e180127
Author(s):  
Renata Ferreira dos Santos ◽  
Danila Fernanda Rodrigues Frias ◽  
Glaucenyra Cecília Pinheiro da Silva ◽  
Talita Ribeiro Silva ◽  
Nivaldo Aparecido de Assis ◽  
...  
Keyword(s):  
Beef Cattle ◽  
High Frequency ◽  
Agglutination Test ◽  
Serological Diagnosis ◽  
Serum Samples ◽  
Mato Grosso ◽  
Leptospira Spp ◽  
Cattle Herds ◽  
Epidemiological Characteristics ◽  
Mato Grosso Do Sul

The epidemiological characteristics of bovine leptospirosis in animals and herds in Mato Grosso do Sul were investigated to determine parameters such as disease frequency and the serovars reactant in beef cattle herds. A total of 4,629 beef cattle herds were examined against 33 Leptospira spp. serovars. The serum samples were submitted to the microscopic agglutination test (MAT) for the serological diagnosis of leptospirosis. The MAT results showed that 3,814 (82.39%) of the 4,629 animals evaluated were seropositive for the bacterium, with serological reactions mainly to serogroup Sejroe, serovar Wolffi (36.49%). The observed high frequency of reactive animals demonstrates the relevance of the infection. Therefore, general and specific measures should be implemented to contain and/or prevent infection of the animals in the studied region.

Prevalence of vesivirus in a laboratory-based set of serum samples obtained from dairy and beef cattle

2006 ◽  
Vol 67 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Andreas Kurth ◽  
James F. Evermann ◽  
Douglas E. Skilling ◽  
David O. Matson ◽  
Alvin W. Smith
Keyword(s):  
Beef Cattle ◽  
Serum Samples

scholarly journals Bovine alphaherpesvirus 1 and 5 in semen from bulls presenting genital lesions under field conditions in Brazil

2019 ◽  
Vol 71 (1) ◽  
pp. 197-203
Author(s):  
A. Henzel ◽  
P.F. Salla ◽  
A.K. Mascitti ◽  
M. Demoliner ◽  
M.C. Solyman ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Strong Evidence ◽  
Genital Tract ◽  
Neutralizing Antibodies ◽  
Neurological Diseases ◽  
Field Conditions ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Viral Dna ◽  
Cattle Farms

ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing serology negative. BoHV-1/5 DNA was detected in the semen of three bulls; one of the them presenting BoHV-1, one out three presenting BoHV-5 and one BoHV-1/5.co-infection All BoHV DNA positive samples came from animals presenting posthitis and other genital lesions at sampling. Results showed a high seroprevalence of BoHV-1/5 antibodies in bulls as well as strong evidence that these viruses are actively circulating in the cattle farms. A remarkable finding is that in the presence of clinically evident lesions in the genital tract, both BoHV-1 and 5 may found in semen.

scholarly journals Neospora caninum: Seroprevalence in beef cattle in the mountainous region of Santa Catarina, Brazil

2017 ◽  
Vol 38 (1) ◽  
pp. 273
Author(s):  
Mayckon Antonio Cardoso Padilha ◽  
Gefferson Wasen ◽  
Antonio Pereira de Souza ◽  
Viviane Milczewski ◽  
Thaís Vaz Brito da Luz ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Neospora Caninum ◽  
Age Groups ◽  
Antibody Test ◽  
Fisher Exact Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Chi Square ◽  
Exact Test ◽  
Immunofluorescence Antibody Test

The distribution of Neospora caninum, an important agent of reproductive disorders in cattle, has been frequently reported in dairy cattle in Brazil and worldwide. Records of infection are less frequent in beef cattle. To determine the seroprevalence of bovine neosporosis, 507 beef cattle blood samples were collected from January 2013 to September 2015, from 16 municipalities of the Associação dos Municípios da Região Serrana (AMURES). Samples were tested for the presence of IgG antibodies ( ? 1:100) against N. caninum by indirect immunofluorescence antibody test (IFAT). Information about sex, age, and origin of the bovines were obtained from the Brazilian Bovine and Bubaline Identification and Certification System (SISBOV), and were tabulated for statistical analysis (Chi-square and Fisher Exact test, P ? 0.05). Of the 507 serum samples analyzed, 70 (13.81%) contained antibodies against N. caninum with titers of 1:100 (16), 1:200 (22), 1:400 (17), 1:800 (nine), 1:1600 (four) and 1:3200 (two). Positive specimens were collected from 13 of the 16 municipalities and across all age groups. Of the positive samples, there were no statistical differences between males and females (32.86% vs. 67.14%, P= 0.1072), age groups (P= 0.4116), or municipalities (P= 0.6838). While not statistically significant (P>0.05), higher seroprevalence was observed among older females. Although seroprevalence was relatively low, results indicate that infection by N. caninum in beef cattle is widespread in the studied region.

scholarly journals Seroprevalence and Molecular Characterization of Coxiella burnetii in Cattle in the Republic of Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 890
Author(s):  
Sunwoo Hwang ◽  
Hyung-Chul Cho ◽  
Seung-Uk Shin ◽  
Ha-Young Kim ◽  
Yu-Jin Park ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Dairy Cattle ◽  
Coxiella Burnetii ◽  
Cattle Breed ◽  
Republic Of Korea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Growth Type ◽  
Serum Samples ◽  
Grazing Cattle ◽  
The Republic

This study was conducted to determine the prevalence of Coxiella burnetii in cattle and how that prevalence is influenced by cattle breed and growth type. A total of 491 cattle [cattle breed: 216 dairy cattle and 275 beef cattle; growth type: indoor housed (n = 294) and grazing (n = 197)] were used. The presence of C. burnetii DNA and antibodies was detected from blood and serum samples using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The overall prevalence of C. burnetii was: 10.8% (95% CI: 8.0–13.5%) using PCR and 8.8% (95% CI: 6.3–11.3%) using ELISA. The prevalence of C. burnetii was significantly higher in beef cattle than in dairy cattle using both PCR (13.5% vs. 7.4%; P = 0.032) and ELISA (14.5% vs. 1.4%; P = 0.000), respectively. Comparison by growth type revealed that C. burnetii infection was significantly higher in grazing cattle than in housed cattle when using both PCR (24.9% vs. 1.4%; P = 0.000) and ELISA (21.3% vs. 0.3%; P = 0.000). Beef cattle were at a significantly higher risk of contracting C. burnetii compared with dairy cattle (odds ratio = 3.20, 95% CI: 1.80–5.67; P = 0.000). The risk of contracting C. burnetii in grazing cattle was increased by 32.57-fold (95% CI: 12.84–82.61; P = 0.000) compared with indoor housed cattle. The phylogenetic analysis based on the IS1111 gene revealed that our sequences grouped with human, tick, goat, and cattle isolates/strains found in several countries. C. burnetii sequences circulating in the Republic of Korea exhibit genetic variations. Thus, grazing is a high risk factor for the prevalence and transmission of C. burnetii.

scholarly journals Serological occurrence for tick-borne agents in beef cattle in the Brazilian Pantanal

2020 ◽  
Vol 29 (1) ◽  
Author(s):  
Inalda Angélica de Souza Ramos ◽  
Victória Valente Califre de Mello ◽  
Natalia Serra Mendes ◽  
Diego Carlos de Souza Zanatto ◽  
João Bosco Vilela Campos ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Fluorescent Antibody ◽  
Single Agent ◽  
Antibody Test ◽  
Babesia Bovis ◽  
Igg Antibodies ◽  
Babesia Bigemina ◽  
Serum Samples ◽  
Serological Reaction ◽  
Brazilian Pantanal

Abstract This study investigated the seropositivity for five different tick-borne agents, namely Anaplasma marginale, Babesia bovis, Babesia bigemina, Coxiella burnetii, Anaplasma phagocytophilum, and Trypanosoma vivax in beef cattle in the Brazilian Pantanal. The serum samples collected from animals (200 cows; 200 calves) were used in indirect enzyme-linked immunosorbent assays (iELISA) to detect IgG antibodies against A. marginale, B. bovis, B. bigemina, and T. vivax, and Indirect Fluorescent Antibody Test (IFAT) for detecting IgG antibodies against C. burnetii and A. phagocytophilum. No correlation was observed between seropositivity for C. burnetii and A. phagocytophilum with other agents whereas moderate correlation was observed for A. marginalexB. bigemina x B. bovis. Cows were more seropositive for T. vivax whereas calves were more seropositive for B. bovis and B. bigemina. The highest number of seropositive animals by a single agent was observed for T. vivax (15.2%). Co-seropositivity for T. vivax + A. marginale was higher in cows (25.5%) and for T. vivax + B. bovis + B. bigemina + A. marginale was higher in calves (57.5%). The high seropositivity correlation for A. marginale x B. bovis x B. bigemina is probably due to the presence of the tick biological vector, Rhipicephalus microplus, in the studied farms. Common transmission pathways, mediated by hematophagous dipterans and fomites, may explain the high co-seropositivity of cows for A. marginale and T. vivax. Low seropositivity to C. burnetii is probably due to the type of breeding system employed (extensive). Seropositivity for A. phagocytophilum in only one animal suggests the occurrence of a cross-serological reaction with another agent of the genus Anaplasma.

Anticardiolipin Antibodies Are Elevated in HIV-1 Infected Haemophiliacs but Do Not Predict for Disease Progression

1989 ◽  
Vol 61 (01) ◽  
pp. 081-085 ◽  
Author(s):  
Simon Panzer ◽  
Christoph Stain ◽  
Hubert Hartl ◽  
Robert Dudczak ◽  
Klaus Lechner
Keyword(s):  
Normal Values ◽  
Anticardiolipin Antibodies ◽  
Haemophilia A ◽  
Serum Samples ◽  
Factor Viii Concentrates ◽  
Patient Groups ◽  
Anti Hiv ◽  
Hiv 1 ◽  
Cd4 Lymphocytes

SummaryLevels of anticardiolipin antibodies (ACA) were measured in 55 patients with haemophilia A in serum samples obtained in 1983 and in 1987. Twenty-one patients were negative for anti HIV-1 antibodies in 1983 and remained negative in 1987; 34 patients had anti HIV-1 antibodies in 1983; 17 of these latter patients remained asymptomatic, whereas 17 patients developed ARC or AIDS during the 4 years follow-up. Thirteen anti HIV-1 negative patients had elevated ACA levels in 1983; subsequently, a significant decrease was observed in all these subjects (p <0.001). All anti HIV-1 positive patients had elevated ACA levels in 1983; normal values were found in 9 patients in 1987. Yet, these changes were not significant (p >0.05). ACA levels were significantly higher in HIV-1 infected patients than in those without anti HIV-1 antibodies (p <0.05). There was no difference of ACA levels between the two anti HIV-1 positive patient groups, be it in 1983 or be it in 1987 (p >0.05). There was no correlation of ACA levels with serum IgG concentrations, CD4+ lymphocytes, or the consumption of factor VIII concentrates.

Immunologic Assessment of Patients Treated with Bovine Fibrin as a Hemostatic Agent

1996 ◽  
Vol 76 (06) ◽  
pp. 0925-0931 ◽  
Author(s):  
John F Carroll ◽  
Keith A Moskowitz ◽  
Niloo M Edwards ◽  
Thomas J Hickey ◽  
Eric A Rose ◽  
...  
Keyword(s):  
Coagulation Factors ◽  
Allergic Reactions ◽  
Factor V ◽  
Normal Range ◽  
Serum Samples ◽  
Human Fibrinogen ◽  
Bovine Thrombin ◽  
Thrombotic Complications ◽  
The Mean ◽  
Clinically Significant

SummaryTwenty-one cardiothoracic surgical patients have been treated with fibrin as a topical hemostatic/sealing agent, prepared from bovine fibrinogen clotted with bovine thrombin. Serum samples have been collected before treatment with fibrin and postoperatively between 1 and 9 days, 3 and 12 weeks, and 6 and 8 months. The titers of anti-bovine fibrinogen antibodies, measured by ELISA specific for immunoglobulins IgG or IgM, increased to maximal values after about 8 or 6 weeks, respectively. After 8 months, IgG titers were on average 20-fold lower than the mean maximal value, while IgM titers returned to the normal range. IgG was the predominant anti-bovine fibrinogen immunoglobulin as documented by ELISA, affinity chromatography and electrophoresis. Anti-bovine fibrinogen antibodies present in patients reacted readily with bovine fibrinogen, but did not cross-react with human fibrinogen as measured by ELISA or by immunoelectrophoresis. A significant amount of antibodies against bovine thrombin and factor V has been found, many cross-reacting with the human counterparts. No hemorrhagic or thrombotic complications, or clinically significant allergic reactions, occurred in any patient, in spite of antibody presence against some bovine and human coagulation factors. The treatment of patients with bovine fibrin, without induction of immunologic response against human fibrinogen, appeared to be an effective topical hemostatic/sealing measure.

Autoantibodies to Thrombomodulin: Development of an Enzyme Immunoassay and a Survey of their Frequency in Patients with the Lupus Anticoagulant

1992 ◽  
Vol 67 (05) ◽  
pp. 507-509 ◽  
Author(s):  
John Gibson ◽  
Margaret Nelson ◽  
Ross Brown ◽  
Hatem Salem ◽  
Harry Kronenberg
Keyword(s):  
Enzyme Immunoassay ◽  
Lupus Anticoagulant ◽  
Specific Binding ◽  
Technical Problem ◽  
Serum Samples ◽  
Citrate Buffer ◽  
The Mean ◽  
Sample Dilution ◽  
Negative Population ◽  
Serum Components

SummaryIn order to investigate the possibility that autoantibodies to thrombomodulin (TM) may exist in patients with the lupus anticoagulant (LA) and perhaps be implicated in the pathogenesis of recurrent thrombosis seen in such patients, we developed an enzyme-immunoassay to screen serum samples for anti-human TM activity. The major technical problem encountered in developing this assay was to reduce the non-specific binding of serum components from both the LA positive and the negative population. Considerable reduction of non-specific binding was achieved by use of a phosphate/citrate buffer at pH 8.0 and the use of an optimal sample dilution of 1/40. In addition, samples were always tested in parallel in blank wells and results are expressed as an OD ratio. Samples from 113 patients with the LA were assayed and compared to 78 patients referred for LA testing but found to be negative. The mean OD values for the LA positive patients (± SD) was 1.36 (0.44) with a range of 0.78-2.57. This was virtually identical to the values for the LA negative population (1.38 ± 0.40, range 0.76-2.77). The results of this study indicate that there is no evidence for the presence of a significant autoantibody activity to TM in patients with the LA when compared to LA negative patients. If such autoantibodies do exist their frequency must be quite low.

Study the Relationship Between Estrogen Hormone and Calcium in Normal Females Before and After Menopause in Baghdad / Iraq

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Sameerah Mustafa ◽  
Asal Tawfeeq ◽  
Hadeel Hasan
Keyword(s):  
Oral Contraceptive ◽  
Healthy Controls ◽  
Serum Samples ◽  
Oral Contraceptive Pills ◽  
Contraceptive Pills ◽  
Menopausal Women ◽  
Before And After ◽  
Group A ◽  
Group B ◽  
Age Range

This study involved the collection of (90) samples of women serum which included (30) serum samples collected from women before menopause (reproductive women) in the age range of (22-43) years and were considered as (group A- control). While, (group B) included (30) serum samples collected from women using oral contraceptive pills between the ages of (22-43) years old. Whereas, another (30) serum samples were collected from women after menopause between the ages of (43-54) years and were considered as (group C). All of the collected serum samples were subjected to a number of serological and chemical tests for the measurement of (E2, HDL, LDL and Ca). Then, the obtained data were statistical analyzed and results showed a significant decrease (p˂ 0.05) in (E2 ,Ca and HDL) levels in menopausal women compared to that of the normal healthy controls. While, there were non-significant decrease (p> 0.05) in (E2, Ca and HDL) levels in women taking oral contraceptive when compared to the normal healthy controls. On the other hand, a significant increase (p˂ 0.05) was recorded in LDL level in menopausal women compared to that of the normal healthy controls whereas, no-significant increase (p˃ 0.05) in the LDL level in women taking oral contraceptives when compared to the control women.

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