scholarly journals Isolation and characterization of hydrocarbon-degrading fungi from Ogbe-Ijoh oil creek, Warri, Delta State, Nigeria

2018 ◽  
Vol 29 (2) ◽  
pp. 72-76
Author(s):  
Faith Iguodala Akinnibosun ◽  
Abigail Ashegbare
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Ph Value ◽  
Total Carbon ◽  
Isolation And Characterization ◽  
Fungal Isolates ◽  
Hydrocarbon Content ◽  
Biodegradation Study ◽  
Fusarium Sp ◽  
Aspergillus Sp

Abstract This study was aimed at isolating and characterizing hydrocarbon-degrading fungi from hydrocarbon-contaminated soil in Ogbe-Ijoh oil creek in Warri, Delta State, Nigeria. Isolation and characterization were carried out using standard methods. Biodegradation assay was carried out over 25 days and monitored using the following parameters; pH, total organic carbon, and total hydrocarbon content. The fungi isolated were: Penicillium sp., Aspergillus niger and Fusarium sp., and these were used for the biodegradation study. Physico-chemical analysis of the crude oil contaminated soil during degradation showed that there was significant decrease in pH during degradation from day 10 - 25 (p ≥ 0.05). This showed the ability of the fungal isolates to utilize crude oil as carbon source, producing acid during its metabolism. The highest pH value during degradation by the fungal isolates was recorded in Fusarium sp. and the lowest was recorded in Aspergillus sp. The fungal isolates were able to reduce the total carbon and hydrocarbon content during degradation. This was probably due to the utilization of the nutrients in the soil sample as energy source. The results from this study indicate the potential of the isolated fungi for hydrocarbon bioremediation activity.

scholarly journals Isolation and characterization of diethyl phthalate degrading bacteria from crude oil contaminated soil

2019 ◽  
Vol 40 (3) ◽  
pp. 275-282
Author(s):  
P.K. Çevik ◽  
◽  
A.B. Eroğlu ◽  
G. Yildizli ◽  
D. Coşan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Diethyl Phthalate ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.

Gravimetric Profile of Hydrocarbon Degrading Bacterial and Fungal Isolates from Contaminated Soil Samples in Ado-Ekiti, Nigeria

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Olayinka O. Idris ◽  
◽  
Olayinka T. Ogunmefun ◽  
Cinderella N. Tuesimi
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Soil Samples ◽  
Engine Oil ◽  
Water Retention Capacity ◽  
Trichoderma Spp ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Spent Engine Oil

One of the biological compounds limiting soil water retention capacity is oil when present due to its hydro-nature. However, some microorganisms exhibit the capacity to degrade oil as a source of carbon, whereby the soil quality is retained and enhanced. Hence, the gravimetric profile of hydrocarbon degrading bacteria and fungi isolated from oil contaminated soil samples was investigated. Soil samples were collected from surface and 10m depth from six different mechanic workshops and generator sites. The pour plate technique was used to isolate the microorganisms. All pure isolates were sub-cultured using Bushnell Haas agar and the isolated bacteria were identified by their morphological and biochemical characteristics. The soil samples pH range was 4.3 - 6.4. Bacteria isolated included Pseudomonas spp., Staphylococcus spp., Microccocus spp., Acinetobacter spp., and Bacillus spp. The fungi isolated included Aspergillus spp., Rhizopus spp., Candida spp., Trichoderma spp. and Penicillium spp. Degradation of kerosene, diesel, crude oil, engine oil, and spent engine oil was allowed using Acinetobacter baumanni, P. aeruginosa, B. subtilis, and S. aureus. Gravimetric analyses were used to determine the percentage of petroleum hydrocarbon degraded by bacterial isolates. The highest percentage of degradation was between P. aeruginosa and B. subtilis. Pseudomonas aeruginosa degraded 97.4% diesel, 88.2% kerosene, 71.3% crude oil, 80.7% engine oil and 78.2% spent engine oil; while Bacillus subtilis degraded 71% diesel, 97% kerosene, 89.6% crude oil, 87% engine oil and 72.6% spent engine oil. This study revealed that bacterial and fungal isolates from oil contaminated soils exhibited the potentials to degrade oil and bioremediation using these microorganisms was possible.

scholarly journals Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

2020 ◽  
Vol 10 (12) ◽  
pp. 4173 ◽  
Author(s):  
Runkai Wang ◽  
Baichun Wu ◽  
Jin Zheng ◽  
Hongkun Chen ◽  
Pinhua Rao ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Ph Value ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Total Petroleum Hydrocarbons ◽  
Impact Factors ◽  
Growth Media ◽  
Bacterial Strains ◽  
Isolation And Characterization

In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the genera Bacillus, Acinetobacter, Sphingobium, Rhodococcus, and Pseudomonas, respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 °C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains’ biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

scholarly journals ISOLATION AND CHARACTERIZATION OF LIPOLYTIC BACTERIA FROM OIL CONTAMINATED SOIL FROM PETROL BUNK AT SOUTHEAST BANGALORE.

2019 ◽  
Vol 7 (2) ◽  
pp. 30-34
Author(s):  
Ajay Kumar Sahu ◽  
Rahul Nemani ◽  
Prangya Prangya Acharya ◽  
Rupali Sinha ◽  
Subhranil Sengupta ◽  
...  
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Lubricating Oil ◽  
Contaminated Sites ◽  
Gravimetric Analysis ◽  
Morphological Studies ◽  
Isolation And Characterization

The isolation of oil contaminated sites and gravimetric analysis of degradation in which, two bacterial formed maximum clearing zones on minerals salt medium. An increase in cell number indicating that the bacterial isolation was responsible for the oil degradation .the collected oil contaminated sites at kormongala, Indra nagar, MTTC culture , micrococcus spp., Bacillus spp., pseudomonas spp., which are able to utilize the oil in soil as carbon sources, were added to oil contaminated soil sample , the growth profiles were determined by monitoring the optical density, dry weight and Ph of the culture utilizing lubricating oil as sole sources of carbon, Bushnell  Haas media supplemented with petrol, kerosene and diesel as sole carbon sources was used for isolation of bacteria capable of degrading these petroleum fractionates. From three soil sample and two water sample, a total of nine bacterial strains were isolated capable of degrading petrol, kerosene and diesel with varying tolerance capacities, the isolates were identify by using standard biochemical test and morphological studies and it was determined that these strains belong to six bacterial genera .the present study suggest that the isolated bacterial species could be employed for bioremediation in environment polluted with petroleum and its products, indigenously from the soil and water contaminated with crude oil in the vicinity of oil drilling well were found to be most efficient crude oil utilize as turbidity observed by spectrophotometrically. In the various study of lipolytic bacteria concluded that the taken of oil contaminated soil from from petroleum bunk and to identify their biochemical characterization by using various sources. How it’s helpful for characterize by using of lipolytic bacteria

scholarly journals Crystal Formation In Cultures Of Fungi : New Records From Bangladesh

2015 ◽  
Vol 41 (1) ◽  
pp. 59-66
Author(s):  
Shamim Shamsi ◽  
Najmun Naher ◽  
Yasmin Fatema
Keyword(s):  
New Records ◽  
Crystal Formation ◽  
First Report ◽  
Sterile Culture ◽  
Penicillium Sp ◽  
Fungal Isolates ◽  
Fusarium Sp ◽  
Aspergillus Sp

In the present study crystal formations were detected in the culture of Aspergillusniger,A. fluvus, Aspergillus sp., Bipolarissorokiniana, Cladosporium spp., Colletotrichumgloeosporioides, Colletotrichumorbiculare,Curvularia spp., Fusarium sp., Paecilomycesspp., Penicillium sp., Pestalotia sp., Pestalotiopsisguepinii, Phaeoisariopsispersonata,Trichodermaviride and seven sterile culture of fungi. Eighteen types of crystallographicstructures were detected from different fungal isolates. This is the first report offormation of crystal in fungal cultures from Bangladesh. Asiat. Soc. Bangladesh, Sci. 41(1): 59-66, June 2015

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2018 ◽  
Vol 23 (4) ◽  
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

<p>This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: <em>Bacillus substilis</em> and <em>Pseudomonas sp</em>. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5    (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.</p>

scholarly journals The Impact of Egbu Abattoir Wastes on Fungal Concentrates of the Soil Environment

2020 ◽  
pp. 1-5
Author(s):  
M. I. Nwachukwu ◽  
C. C. Ike ◽  
I. O. Nwachukwu
Keyword(s):  
Contaminated Soil ◽  
Control Soil ◽  
Soil Environment ◽  
Standard Methods ◽  
Candida Sp ◽  
Invading Species ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
The Impact

The impact of Egbu abattoir wastes on fungal concentrates of the soil environment was undertaken. Samples of contaminated soil from the abattoir environment were taken against control and evaluated for fungal concentrates using standard methods. Results obtained showed that total fungal count (TFC) ranged from 3.5×105 cfu/g to 4.50×105 cfu/g and total hydrocarbon utilizing fungi (THUF) ranged from 1.8×105 cfu/g to 3.80×105 cfu/g.  The percentage occurrence for mould isolates indicated the presence of Absidia sp., Aspergillus sp., Fusarium sp., Cladosporium sp., Penicillium sp., and Rhizopus sp. while yeast isolates were Candida sp., Rhodotorula sp., Saccharomyces sp., and Torulopsis sp., for both seasons. Though most of these organisms were indigenous to the soil some invading species were also isolated and were higher in contaminated soil than control soil. Egbu abattoir waste could be behind these observations. The study has revealed the impact of Egbu abattoir wastes on fungal concentrates of the soil environment.

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