The extract from hop cones in plasma protects against changes following exposure to peroxynitrite

2011 ◽  
Vol 6 (6) ◽  
pp. 990-996 ◽  
Author(s):  
Beata Olas ◽  
Joanna Kołodziejczyk-Czepas ◽  
Barbara Wachowicz ◽  
Dariusz Jędrejek ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Plasma Lipid ◽  
Humulus Lupulus ◽  
Raw Material ◽  
Brewing Industry ◽  
Coagulation Activity ◽  
Medicinal Properties ◽  
Plasma Lipid Peroxidation ◽  
Antioxidative Action

AbstractHumulus lupulus (Cannabaceae) is well known throughout the world as a raw material in the brewing industry. The antioxidative action of hop cones is poorly understood, therefore the aim of our present study was to investigate in vitro changes in human plasma induced by peroxynitrite in the presence of the highly purified extract from hop cones (Humulus lupulus). The aim of our study was also to explain the effect of the extract from hop cones on coagulation activity of human plasma treated with peroxynitrite. The action of the extract from hop cones was compared with the properties of a well-characterized commercial monomeric polyphenol — resveratrol (3,4′,5-trihydroxystilbene). The tested plant extract, like resveratrol, significantly inhibited protein carbonylation and nitration in plasma treated with ONOO−(0.1 mM). The extract from hop cones, like resveratrol, also caused a distinct reduction of plasma lipid peroxidation induced by ONOO−. Moreover, the tested extract modulated the coagulation properties of plasma treated with peroxynitrite. It seems that antioxidative activities of the highly purified extract from hop cones may be responsible for its medicinal properties.

Epicatechin Inhibits Human Plasma Lipid Peroxidation Caused by Haloperidol In Vitro

2011 ◽  
Vol 37 (3) ◽  
pp. 557-562 ◽  
Author(s):  
Anna Dietrich-Muszalska ◽  
Bogdan Kontek ◽  
Beata Olas ◽  
Jolanta Rabe-Jabłońska
Keyword(s):  
Lipid Peroxidation ◽  
Human Plasma ◽  
Plasma Lipid ◽  
Plasma Lipid Peroxidation

scholarly journals Quetiapine, Olanzapine and Haloperidol Affect Human Plasma Lipid Peroxidation in vitro

2011 ◽  
Vol 63 (4) ◽  
pp. 197-201 ◽  
Author(s):  
Anna Dietrich-Muszalska ◽  
Bogdan Kontek ◽  
Jolanta Rabe-Jabłońska
Keyword(s):  
Lipid Peroxidation ◽  
Human Plasma ◽  
Plasma Lipid ◽  
Plasma Lipid Peroxidation

scholarly journals The protective effects of selenoorganic compounds against peroxynitrite-induced changes in plasma proteins and lipids

2006 ◽  
Vol 11 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Paweł Nowak ◽  
Joanna Saluk-Juszczak ◽  
Beata Olas ◽  
Joanna Kołodziejczyk ◽  
Barbara Wachowicz
Keyword(s):  
Lipid Peroxidation ◽  
Human Plasma ◽  
Plasma Proteins ◽  
Enzyme Inhibitors ◽  
Group Formation ◽  
Plasma Lipid ◽  
Protective Effects ◽  
Induced Changes ◽  
Plasma Lipid Peroxidation

AbstractMany selenoorganic compounds play an important role in biochemical processes and act as antioxidants, enzyme inhibitors or drugs. The effects of a new selenocompound — bis(2-aminophenyl)-diselenide on oxidative/nitrative changes in human plasma proteins induced by peroxynitrite (ONOO−) were studied in vitro and compared with the those of ebselen, a well-known antioxidant. We also studied the role of the tested selenocompounds in peroxynitrite-induced plasma lipid peroxidation. Exposure of the plasma to peroxynitrite (0.1 mM) resulted in an increase in the level of carbonyl groups and nitrotyrosine residues in plasma proteins (estimated using the ELISA method and Western blot analysis). In the presence of different concentrations (0.025–0.1 mM) of the tested selenocompounds, 0.1 mM peroxynitrite caused a distinct decrease in the level of carbonyl group formation and tyrosine nitration in plasma proteins. Moreover, these selenocompounds also inhibited plasma lipid peroxidation induced by ONOO−1 (0.1 mM). The obtained results indicate that in vitro bis(2-aminophenyl)-diselenide and ebselen have very similar protective effects against peroxynitrite-induced oxidative/nitrative damage to human plasma proteins and lipids.

scholarly journals Comparative Phytochemical, Antioxidant, and Hemostatic Studies of Extract and Four Fractions from Paulownia Clone in Vitro 112 Leaves in Human Plasma

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4371
Author(s):  
Weronika Adach ◽  
Jerzy Żuchowski ◽  
Barbara Moniuszko-Szajwaj ◽  
Malgorzata Szumacher-Strabel ◽  
Anna Stochmal ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Phenolic Compounds ◽  
Human Plasma ◽  
Antioxidant Properties ◽  
Plasma Lipid ◽  
Inhibitory Effect ◽  
Climate Conditions ◽  
Positive Effects ◽  
Chemical Content

Background: The Paulownia Clone in Vitro 112, known as oxytree or oxygen tree, is a hybrid clone of the species Paulownia elongata and Paulownia fortunei (Paulowniaceae). The oxytree is a fast-growing hybrid cultivar that can adapt to wide variations in edaphic and climate conditions. In this work, Paulownia Clone in Vitro 112 leaves were separated into an extract and four fractions (A–D) differing in chemical content in order to investigate their chemical content using LC-MS analysis. The extract and fractions were also evaluated for their anticoagulant and antioxidant properties in a human plasma in vitro. Results: The Paulownia leaf extract contained mainly phenolic compounds (e.g., verbascoside), small amounts of iridoids (e.g., aucubin or 7-hydroxytometoside) and triterpenoids (e.g., maslinic acid) were also detected. Our results indicate that the extract and fractions have different effects on oxidative stress in human plasma treated with H2O2/Fe in vitro, which could be attributed to differences in their chemical content. For example, the extract and all the fractions, at the two highest concentrations of 10 and 50 µg/mL, significantly inhibited the plasma lipid peroxidation induced by H2O2/Fe. Fractions C and D, at all tested concentrations (1–50 µg/mL) were also found to protect plasma proteins against H2O2/Fe-induced carbonylation. The positive effects of fraction C and D were dependent on the dose. Conclusions: The extract and all four fractions, but particularly fractions C and D, which are rich in phenolic compounds, are novel sources of antioxidants, with an inhibitory effect on oxidative stress in human plasma in vitro. Additionally, the antioxidant potential of fraction D may be associated with triterpenoids.

Antioxidant Activities of Soluble Dietary Fiber Extracted from Brewers’ Spent Grain

2011 ◽  
Vol 233-235 ◽  
pp. 2824-2827 ◽  
Author(s):  
Quan Yi Fu ◽  
Xu Cong Yu ◽  
Lin Li ◽  
Guo Qin Liu ◽  
Bing Li
Keyword(s):  
Dietary Fiber ◽  
Superoxide Anion ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Raw Material ◽  
Dpph Radical ◽  
Brewing Industry ◽  
Soluble Dietary Fiber ◽  
Spent Grain

Brewers’ spent grain (BSG) is the most common by-product of the brewing industry, contains about 16.8% cellulose, 28.4% non-cellulosic polysaccharides and 27.8% lignin, which is a good raw material for industrial production of soluble dietary fiber (SDF).The antioxidant activity of crude dietary fiber (CDF) and grade dietary fiber (GDF) isolated from BSG was investigated in vitro. The scavenging hydroxyl radical capability of CDF and GDF was respectively reached to15.4% and 69.2% at the concentration of 0.1mg /mL. DF concentration from 0.02mg /mL to 0.1m g /mL was used. The scavenging capability of CDF and GDF to superoxide anion radical was increased from 10.68% and 12.33% to 35.78% and 78.39%. Both of the two DF showed some effect in scavenging superoxide radical and 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical with the concentration increase. The result showed that BSG DF can be used as a good additive with some functionality.

The in Vitro Anticoagulant Activity of Human Plasma Lipids

1965 ◽  
Vol 13 (02) ◽  
pp. 531-542
Author(s):  
J Cohen ◽  
C. F Reed ◽  
S. B Troup
Keyword(s):  
Human Plasma ◽  
Inhibitory Activity ◽  
Plasma Lipids ◽  
Present Report ◽  
Anticoagulant Activity ◽  
Lipid Fraction ◽  
Plasma Lipid ◽  
Lipid Mixture ◽  
Platelet Poor Plasma

SummaryThe present report describes the in vitro inhibition of prothrombin consumption by lipid extracted from normal human platelet-poor plasma. Pro-coagulant lipids and the test plasma lipids were dissolved in the same solvent and evaporated to dryness. The dried lipid mixture was emulsified in platelet-poor plasma, and the plasma clotted by recalcification. Inhibition of prothrombin consumption by plasma lipid was observed when the ratio of plasma lipid to procoagulant lipid equaled or exceeded 5:1. The inhibitory effect of plasma lipid on prothrombin consumption was not observed when procoagulant activity was provided by intact platelets or platelet granules.Human plasma lipids have been fractionated on silicic acid columns to permit identification of the plasma lipid component responsible for the inhibition of prothrombin consumption. The inhibitory activity is present in a lipid fraction which is 95% lecithin. Other plasma lipid components exhibit little or no inhibitory activity.

scholarly journals Composition and In Vitro Effects of Cultivars of Humulus lupulus L. Hops on Cholinesterase Activity and Microbial Growth

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1377 ◽  
Author(s):  
Joanna Kobus-Cisowska ◽  
Daria Szymanowska-Powałowska ◽  
Oskar Szczepaniak ◽  
Dominik Kmiecik ◽  
Monika Przeor ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Ethanol Extract ◽  
Humulus Lupulus ◽  
Raw Material ◽  
In Vitro Tests ◽  
Humulus Lupulus L ◽  
Health Promoting ◽  
In Vitro Effects ◽  
Ethanol Extracts

Common hop (Humulus lupulus L.) has significant health-promoting properties. Hop cones contain resins, essential oils, proteins, polyphenols, lipids, waxes, and cellulose. Hop extracts include bioactive compounds such as polyphenolic compounds (phenolic acids, and flavonols), and chlorophylls. The aim of this study was to compare the pro-health potential of hop cone extracts obtained from three cultivars (Magnum, Lubelski, and Marynka). The results showed that the cones of Magnum cultivar demonstrated the highest biological activity. The sum of phenolic acids and flavonols in ethanol extract was the highest for this variety and was equal 4903.5 µg/g dw. Ethanol extracts of Magnum cultivars showed the highest degree of iron ion chelation (55.43–88.76%) as well as the activity against 1,1-diphenyl-2-picrylhydrazyl radical (4.75 mmol Tx/g dw). Hop cone extracts as cholinesterase inhibitors showed high potential for aqueous variants. In terms of antimicrobial activity, all investigated extracts demonstrated strong inhibition against Staphylococcus aureus and Staphylococcus epidermidis, with the Magnum cultivar showing the strongest inhibition. Owing to the biofunctional features of hop cone, it can be concluded that it is an attractive raw material with pro-health potential that can be used much more widely in food technology. However, it should be noted that toxicological tests and in vitro tests must be carried out before the raw material is used in food production.

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