Effect of environmental conditions and water status on the bioactive compounds of broccoli

2013 ◽  
Vol 8 (8) ◽  
pp. 777-787 ◽  
Author(s):  
Zoltán Pék ◽  
Hussein Daood ◽  
Magdolna Nagyné ◽  
András Neményi ◽  
Lajos Helyes

AbstractFour experiments were carried out in 2010 and 2011 to determine how cultivation period (spring or autumn), harvest season (summer or autumn), and plant water status (irrigated or rainfed) influenced content and composition of broccoli cultivar Parthenon F1 with respect to sulforaphane and phenolics under field conditions in Gödöllő, Hungary. Sulforaphane content was significantly higher in the autumn harvests, regardless of irrigation treatments. Harvest season also influenced total phenolics content, with the highest values occurring in the spring season. Harvest season also affected trolox equivalent antioxidant capacity (TEAC) and this capacity was also the greatest in spring. Caffeic acid glucoside was a major phenolics component in both spring and autumn season harvests. The season and irrigation related changes in other phenolic component contents were also characterised in this study.

2015 ◽  
Vol 95 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Cheng Chen ◽  
Ling Han ◽  
Qun-Li Yu ◽  
Ru-Ren Li

Chen, C., Han, L., Yu, Q.-L. and Li, R.-R. 2015. Color stability and antioxidant capacity of yak meat as affected by feeding with pasture or grain. Can. J. Anim. Sci. 95: 189–195. The objective of the present study was to analyze the effect of pasture or grain on color stability and antioxidant capacity of M. longissimus lumborum (LL) from pasture-fed (PF) or grain-fed (GF) yaks. The color stability and metmyoglobin percentage (MetMb%) were determined during 9 d of aerobic refrigerated storage. The antioxidant capacity was estimated by the total phenolics content, Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP). Compared with the GF group, the LL from the PF group showed significantly (P<0.05) higher redness (a* values), with lower decline rate in a* values (P<0.05) over 1 to 7 d of refrigerated storage. The LL from the PF group had a significantly (P<0.05) lower metmyoglobin accumulation rate. At the end of storage, the muscle's MetMb% of the PF and GF group were 46.33 and 56.66%, respectively. The PF group showed significantly higher total phenolics content (+23.94%; P<0.05) in muscles, resulting in greater TEAC and FRAP, which were 24.81 and 3.99% higher than the GF group (P<0.05), respectively. In conclusion, the pasture enhanced antioxidant capacity of yak meat and contributed to improve the meat color stability.


2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Nathalie A. da Silva ◽  
Joyce Kelly R. da Silva ◽  
Eloisa Helena A. Andrade ◽  
Lea Maria M. Carreira ◽  
Pergentino José C. Sousa ◽  
...  

The leaves and fine stems of Lippia schomburgkiana recorded an essential yield of 1.8%, the main constituents of which were 1,8-cineole (64.1%) and α-terpineol (12.0%). The methanol extract of L. schomburgkiana inhibited the DPPH radical, resulting in an EC50 value of 16.1 ± 0.7 μg.mL−1, which is only three times lower than that of trolox (4.7 ± 0.4 μg.mL−1), signifying a high antioxidant activity for the species. The amount of total phenolics (376.7 ± 35.5 mg GAE/g) and the trolox equivalent antioxidant capacity (327.0 ± 24.8 mg TE/g) of the methanol extract confirmed the significant antioxidative capacity of this plant. The brine shrimp bioassay carried out on the oil (49.6 ± 0.4 μg.mL−1) showed high toxicity, providing important evidence of its biological activity. The sensory evaluation of the leaves of L. schomburgkiana showed a percentage acceptance value very close to commercial oregano, indicating that the plant can be used in spice and condiment products.


HortScience ◽  
2012 ◽  
Vol 47 (11) ◽  
pp. 1646-1652 ◽  
Author(s):  
Zoltán Pék ◽  
Hussein Daood ◽  
Magdolna Gasztonyi Nagyné ◽  
Mária Berki ◽  
Marianna Márkus Tóthné ◽  
...  

Two experiments were carried out in 2010 to determine how cultivation period/harvest season, plant water status/irrigated, or rainfed and foliar sulfur supplementation influences the yield, sulforaphane, phenolics content, and polyphenol composition of broccoli cultivar Parthenon. Yield was significantly higher in the fall harvests. Foliar sulfur treatment induced earliness in irrigated broccoli in the fall harvest season of main florets by 44%. Sulforaphane content was higher in the fall harvests regardless of treatments. Harvest season also influenced total polyphenol content with the highest values measured in the spring season. Sulfur treatment in combination with irrigation increased total polyphenol content by 24% in spring and this change was statistically significant. Harvest season also affected trolox equivalent antioxidant capacity (TEAC) values with the highest values (4.3 mmol·kg−1) also measured in spring. In the irrigated treatment alone or irrigated treatment combined with sulfur treatment, spring season values were higher by 24% and 11%, respectively, compared with fall values and the difference between treatments was significant. Caffeic acid glucoside was one of the major phenolic components in both spring and fall season harvests, whereas ferulic acid glucoside could only be detected in spring florets. From the measured phenolic components, gallic acid content was the only one that was significantly raised by foliar sulfur supplementation in both spring and fall harvests in irrigated or rainfed treatments. Maximum gallic acid values (7.1 to 7.2 mg·kg−1) occurred in the irrigated combined with foliar sulfur treatment of the fall harvest in side and main florets, respectively. The season, irrigation, and foliar sulfur supplementation were related changes in other phenolic component contents and are also described in detail.


2020 ◽  
Vol 11 ◽  
Author(s):  
Wojciech Rybiński ◽  
Magdalena Karamać ◽  
Michał Janiak ◽  
Andreas Börner ◽  
Natalia Płatosz ◽  
...  

Grass pea seeds of 30 varieties from Poland, Slovakia, Czech, Russia, Ukraine, Hungary, Bulgaria, India, Iran, Tunisia, Ethiopia, Canada, and Chile were investigated. Phenolic compounds were extracted from seeds into 80% (v/v) methanol. The total phenolics compounds content of the extracts was determined using Folin-Ciocalteu’s phenol reagent. The antioxidant activity was determined using ABTS and FRAP assays. Total phenolic contents ranged from 2.74 to 10.7 mg/g extract and from 35.9 to 144.7 mg/100 g seeds. The extracts and seeds were characterized using Trolox equivalent antioxidant capacity values of 0.014–0.060 mmol Trolox/g extract and 0.188–0.866 mmol Trolox/100 g seeds, and FRAP values of 0.188–0.866 mmol Fe2+/g extract and 0.541–1.398 Fe2+/100 g seeds. The total phenolics content of grass pea extract was correlated with the results of the ABTS (r = 0.854) and FRAP (r = 0.958) assays. A similar correlation existed between the results of both assays (r = 0.890). Phenolic acids were the dominant phenolic compounds of two cultivars of grass pea. Syringic and sinapic acids had the highest amounts while ferulic, p-hydroxybenzoic, protocatechuic, and p-coumaric acids were present in much lower quantitie. Phenolic acids were present in the form of free compounds, esters, and glycosides.


Biomarkers ◽  
2002 ◽  
Vol 7 (4) ◽  
pp. 291-298 ◽  
Author(s):  
Jan W. Dallinga ◽  
Guido R. M. M. Haenen ◽  
Aalt Bast ◽  
Frederik-Jan Van Schooten

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Martina Horvathova ◽  
Zuzana Orszaghova ◽  
Lucia Laubertova ◽  
Magdalena Vavakova ◽  
Peter Sabaka ◽  
...  

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.


2007 ◽  
Vol 20 (3) ◽  
pp. 585-593 ◽  
Author(s):  
G. Cinnella ◽  
G. Vendemiale ◽  
M. Dambrosio ◽  
G. Serviddio ◽  
P.L. Pugliese ◽  
...  

We studied the effects of Propofol, Desflurane, and Sevoflurane on the systemic redox balance in patients undergoing laparohysterectomy. We measured blood concentration of glutathione (GSH), plasma antioxidant capacity (Trolox Equivalent Antioxidant Capacity-TEAC), and lipid peroxidation products (malondialdehyde (aMDA) and 4-hydroxynonenal (aHNE) protein adducts). Sixty patients were randomly placed into three groups of twenty people each. In Group P anesthesia was induced with Propofol 2 mg/kg and maintained with 12–10–8 mg/kg/min; in Groups S and D anesthesia was induced with 3 mg/kg Sodium Thiopental and maintained with 2% Sevoflurane and 6% Desflurane, respectively. Blood samples were collected prior to induction (T0bas), 60min and 24h postoperatively (T160' and T224 h). In Group P, GSH increased on T160' (p<0.02) and returned to baseline on T24h, while TEAC remained unmodified; in Groups S, GSH and TEAC decreased on T160' (p<0.01 vs. T0bas, p<0.03 vs. T0bas, respectively); in Group D, on T160' there was a slight decrease of GSH and TEAC. The levels of aMDA slightly decreased throughout the study periods in Group P, increased in Group D, and remained stable in Group S. Propofol showed antioxidant properties, while Sevoflurane and Desflurane seemed to shift the redox balance towards oxidation, yet without inducing overt oxidative damage.


Author(s):  
José María Giménez-Egido ◽  
Raquel Hernández-García ◽  
Damián Escribano ◽  
Silvia Martínez-Subiela ◽  
Gema Torres-Luque ◽  
...  

The purpose of this paper was to analyze the changes caused by a one-day tennis tournament in biomarkers of oxidative stress and α-amylase in saliva in children. The sample was 20 male active children with the following characteristics: (a) age of players = 9.46 ± 0.66 years; (b) weight = 34.8 ± 6.5 kg; (c) height = 136.0 ± 7.9 cm; (d) mean weekly training tennis = 2.9 ± 1.0 h. The tennis competition ran for one day, with four matches for each player. Data were taken from the average duration per match and the rating of perceived exertion (RPE). Four biomarkers of antioxidant status: uric acid (AU), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of saliva (FRAS, cupric reducing antioxidant capacity (CUPRAC) and salivary alpha-amylase (sAA) as a biomarker of psychological stress were measured in saliva. The time points were baseline (at home before the tournament), pre-competition (immediately before the first match) and post-match (after each match) measurements. The four biomarkers of antioxidant status showed a similar dynamic with lower values at baseline and a progressive increase during the four matches. Overall one-day tennis competition in children showed a tendency to increase antioxidant biomarkers in saliva. In addition, there was an increase in pre-competition sAA possibly associated with psychological stress. Further studies about the possible physiological implications of these findings should be performed in the future.


1990 ◽  
Vol 20 (4) ◽  
pp. 415-427 ◽  
Author(s):  
A. N. Burdett

Both the morphological and physiological characteristics of forest planting stock vary widely with nursery culture and environment. Through the control of environmentally determined variation in phenotype, stock can be adapted to both the stress of transplanting from nursery to forest site and the particular environmental conditions of the forest site. Evidence is discussed that indicates that the stress of transplanting is primarily water stress, resulting from (i) the confinement of roots to the planting hole, (ii) poor root–soil contact, and (iii) low root permeability. These deficiencies are overcome by root growth, which is thus a central process in plantation establishment. Root growth depends largely on current photosynthesis. Photosynthesis depends on the assimilation of carbon dioxide at the expense of lost water in transpiration. Transpiration is limited by water uptake and hence depends on root growth. Root growth and photosynthesis in newly planted trees are thus mutually dependent. Because of this relationship, plant water status immediately after planting, or as soon as conditions favorable to root growth occur, is a crucial factor in determining plantation establishment success. High plant tissue water status immediately after planting, or as soon as environmental conditions permit root growth, allows the onset of a positive cycle of root growth supported by photosynthesis and photosynthesis supported by root growth; whereas low tissue water potential immediately after planting can lead to the inhibition or root growth by a lack of photosynthesis and the inhibition of photosynthesis by a lack of root growth. Stock characteristics that enhance plant water status immediately after planting are reviewed and the scope for their control considered. Stock characteristics affecting adaptation to particular planting site conditions, or capable of affecting postestablishment plantation performance, are also discussed.


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