Effect of Propofol, Sevoflurane and Desflurane on Systemic Redox Balance

2007 ◽  
Vol 20 (3) ◽  
pp. 585-593 ◽  
Author(s):  
G. Cinnella ◽  
G. Vendemiale ◽  
M. Dambrosio ◽  
G. Serviddio ◽  
P.L. Pugliese ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Redox Balance ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Sodium Thiopental ◽  
Blood Samples ◽  
Plasma Antioxidant Capacity ◽  
Trolox Equivalent ◽  
Plasma Antioxidant ◽  
Group D

We studied the effects of Propofol, Desflurane, and Sevoflurane on the systemic redox balance in patients undergoing laparohysterectomy. We measured blood concentration of glutathione (GSH), plasma antioxidant capacity (Trolox Equivalent Antioxidant Capacity-TEAC), and lipid peroxidation products (malondialdehyde (aMDA) and 4-hydroxynonenal (aHNE) protein adducts). Sixty patients were randomly placed into three groups of twenty people each. In Group P anesthesia was induced with Propofol 2 mg/kg and maintained with 12–10–8 mg/kg/min; in Groups S and D anesthesia was induced with 3 mg/kg Sodium Thiopental and maintained with 2% Sevoflurane and 6% Desflurane, respectively. Blood samples were collected prior to induction (T0bas), 60min and 24h postoperatively (T160' and T224 h). In Group P, GSH increased on T160' (p<0.02) and returned to baseline on T24h, while TEAC remained unmodified; in Groups S, GSH and TEAC decreased on T160' (p<0.01 vs. T0bas, p<0.03 vs. T0bas, respectively); in Group D, on T160' there was a slight decrease of GSH and TEAC. The levels of aMDA slightly decreased throughout the study periods in Group P, increased in Group D, and remained stable in Group S. Propofol showed antioxidant properties, while Sevoflurane and Desflurane seemed to shift the redox balance towards oxidation, yet without inducing overt oxidative damage.

A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates

1993 ◽  
Vol 84 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Nicholas J. Miller ◽  
Catherine Rice-Evans ◽  
Michael J. Davies ◽  
Vimala Gopinathan ◽  
Anthony Milner
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reference Population ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Compounds ◽  
Automated Method ◽  
Plasma Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Plasma Antioxidant

1. A new method has been developed for measuring the total antioxidant capacity of body fluids and drug solutions, based on the absorbance of the ABTS*+ radical cation. 2. An automated method for use on a centrifugal analyser, as well as a manual method, is described. 3. The procedure has been applied to physiological antioxidant compounds and radical-scavenging drugs, and an antioxidant ranking was established based on their reactivity relative to a 1.0 mmol/l Trolox standard. 4. The Trolox equivalent antioxidant capacity of plasma from an adult reference population has been measured, and the method optimized and validated. 5. The method has been applied to investigate the total plasma antioxidant capacity of neonates and how this may be compromised in prematurity.

scholarly journals Increased antioxidant capacity in the plasma of dogs after a single oral dosage of tocotrienols

2011 ◽  
Vol 106 (S1) ◽  
pp. S116-S119 ◽  
Author(s):  
Jens Raila ◽  
Sascha Rohn ◽  
Florian J. Schweigert ◽  
Getu Abraham
Keyword(s):  
Antioxidant Capacity ◽  
Oral Dosage ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Plasma ◽  
Beagle Dogs ◽  
Plasma Antioxidant Capacity ◽  
Trolox Equivalent ◽  
Plasma Antioxidant ◽  
Time Point

The intestinal absorption of tocotrienols (TCT) in dogs is, to our knowledge, so far unknown. Adult Beagle dogs (n 8) were administered a single oral dosage of a TCT-rich fraction (TRF; 40 mg/kg body weight) containing 32 % α-TCT, 2 % β-TCT, 27 % γ-TCT, 14 % δ-TCT and 25 % α-tocopherol (α-TCP). Blood was sampled at baseline (fasted), 1, 2, 3, 4, 5, 6, 8 and 12 h after supplementation. Plasma and chylomicron concentrations of TCT and α-TCP were measured at each time point. Plasma TAG were measured enzymatically, and plasma antioxidant capacity was assessed by the Trolox equivalent antioxidant capacity assay. In fasted dogs, levels of TCT were 0·07 (sd 0·03) μmol/l. Following the administration of the TRF, total plasma TCT peaked at 2 h (7·16 (sd 3·88) μmol/l; P < 0·01) and remained above baseline levels (0·67 (sd 0·44) μmol/l; P < 0·01) at 12 h. The TCT response in chylomicrons paralleled the increase in TCT in plasma with a maximum peak (3·49 (sd 2·06) μmol/l; P < 0·01) at 2 h post-dosage. α-TCP was the major vitamin E detected in plasma and unaffected by TRF supplementation. The Trolox equivalent values increased from 2 h (776 (sd 51·2) μmol/l) to a maximum at 12 h (1130 (sd 7·72) μmol/l; P < 0·01). The results show that TCT are detected in postprandial plasma of dogs. The increase in antioxidant capacity suggests a potential beneficial role of TCT supplementation in the prevention or treatment of several diseases in dogs.

scholarly journals HPLC, UV-Vis and NMR spectroscopic and DFT characterization of purpurin isolated from Rubia tinctorum L.

2013 ◽  
Vol 67 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Zoran Markovic ◽  
Nedeljko Manojlovic ◽  
Svetlana Jeremic ◽  
Miroslav Zivic
Keyword(s):  
Antioxidant Capacity ◽  
Colorimetric Assay ◽  
Antioxidant Properties ◽  
Electron Delocalization ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Bond Dissociation Enthalpy ◽  
Rubia Tinctorum ◽  
Dissociation Enthalpy ◽  
Trolox Equivalent

Purpurin (1,2,4-trihidroxyanthraquinone), a naurally occuring anthraquinone pigment present in the root of Rubia tinctorum (Mull. Arg.) Zahlbr. was isolated from the plant, purified and characterized by HPLC chromatography, UV-Vis and NMR spectroscopy. The geometries of the purpurin conformers were optimized using the B3LYP/6-311+G(d,p) level of theory. It was found that all conformations have similar energies and that the corresponding purpurin radicals are planar. In spite of their planarity, there is no significant electron delocalization over the A and C rings. The antioxidant properties of purpurin were investigated using the colorimetric assay as Trolox-equivalent antioxidant capacity, and theoretical BDE (Bond Dissociation Enthalpy). Both methods revealed that purpurin has strong antioxidant capacity.

scholarly journals Attenuation of human hypoxic pulmonary vasoconstriction by acetazolamide and methazolamide

2018 ◽  
Vol 125 (6) ◽  
pp. 1795-1803 ◽  
Author(s):  
Lindsey M. Boulet ◽  
Luc J. Teppema ◽  
Heather K. Hackett ◽  
Paolo B. Dominelli ◽  
William S. Cheyne ◽  
...  
Keyword(s):  
Metabolic Acidosis ◽  
Antioxidant Capacity ◽  
Hypoxic Pulmonary Vasoconstriction ◽  
Acute Mountain Sickness ◽  
Antioxidant Properties ◽  
Systolic Pressure ◽  
Pulmonary Vasoconstriction ◽  
Plasma Antioxidant Capacity ◽  
Vascular Sensitivity ◽  
Plasma Antioxidant

Acetazolamide (AZ), a carbonic anhydrase inhibitor used for preventing altitude illness attenuates hypoxic pulmonary vasoconstriction (HPV) while improving oxygenation. Methazolamide (MZ), an analog of acetazolamide, is more lipophilic, has a longer half-life, and activates a major antioxidant transcription factor. However, its influence on the hypoxic pulmonary response in humans is unknown. The aim of this study was to determine whether a clinically relevant dosing of MZ improves oxygenation, attenuates HPV, and augments plasma antioxidant capacity in men exposed to hypoxia compared with an established dosing of AZ known to suppress HPV. In this double-blind, placebo-controlled crossover trial, 11 participants were randomized to treatments with MZ (100 mg 2× daily) and AZ (250 mg 3× daily) for 2 days before 60 min of hypoxia (FIO2 ≈0.12). Pulmonary artery systolic pressure (PASP), alveolar ventilation (V̇A), blood gases, and markers of redox status were measured. Pulmonary vascular sensitivity to hypoxia was determined by indexing PASP to alveolar PO2. AZ caused greater metabolic acidosis than MZ, but the augmented V̇A and improved oxygenation with hypoxia were similar. The rise in PASP with hypoxia was lower with MZ (9.0 ± 0.9 mmHg) and AZ (8.0 ± 0.7 mmHg) vs. placebo (14.1 ± 1.3 mmHg, P < 0.05). Pulmonary vascular sensitivity to hypoxia (ΔPASP/ΔPAO2) was reduced equally by both drugs. Only AZ improved the nonenzymatic plasma antioxidant capacity. Although AZ had only plasma antioxidant properties, MZ led to similar improvements in oxygenation and reduction in HPV at a dose causing less metabolic acidosis than AZ in humans. NEW & NOTEWORTHY Both acetazolamide and methazolamide are effective in the prevention of acute mountain sickness by inducing an increase in ventilation and oxygenation. Acetazolamide attenuates hypoxic pulmonary vasoconstriction; however, it was previously unknown whether methazolamide has the same effect in humans. This study shows that a dosing of methazolamide causing less metabolic acidosis improves oxygenation while attenuating hypoxic pulmonary vasoconstriction and pulmonary vascular sensitivity to hypoxia. Acetazolamide improved plasma antioxidant capacity better than methazolamide.

scholarly journals Study on the Synthesis, Antioxidant Properties, and Self-Assembly of Carotenoid–Flavonoid Conjugates

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 636
Author(s):  
Ildikó Línzembold ◽  
Dalma Czett ◽  
Katalin Böddi ◽  
Tibor Kurtán ◽  
Sándor Balázs Király ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Self Assembly ◽  
Uv Light ◽  
Antioxidant Properties ◽  
Hydroxyl Group ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Solvent Ratio ◽  
Protective Effects ◽  
Synergistic Enhancement ◽  
Trolox Equivalent

Flavonoids and carotenoids possess beneficial physiological effects, such as high antioxidant capacity, anticarcinogenic, immunomodulatory, and anti-inflammatory properties, as well as protective effects against UV light. The covalent coupling of hydrophobic carotenoids with hydrophilic flavonoids, such as daidzein and chrysin, was achieved, resulting in new amphipathic structures. 7-Azidohexyl ethers of daidzein and chrysin were prepared in five steps, and their azide-alkyne [4 + 2] cycloaddition with pentynoates of 8′-apo-β-carotenol, zeaxanthin, and capsanthin afforded carotenoid–flavonoid conjugates. The trolox-equivalent antioxidant capacity against ABTS•+ radical cation and self-assembly of the final products were examined. The 1:1 flavonoid–carotenoid hybrids generally showed higher antioxidant activity than their parent flavonoids but lower than that of the corresponding carotenoids. The diflavonoid hybrids of zeaxanthin and capsanthin, however, were found to exhibit a synergistic enhancement in antioxidant capacities. ECD (electronic circular dichroism) and UV-vis analysis of zeaxanthin–flavonoid conjugates revealed that they form different optically active J-aggregates in acetone/water and tetrahydrofuran/water mixtures depending on the solvent ratio and type of the applied aprotic polar solvent, while the capsanthin derivatives showed no self-assembly. The zeaxanthin bis-triazole conjugates with daidzein and with chrysin, differing only in the position of a phenolic hydroxyl group, showed significantly different aggregation profile upon the addition of water.

scholarly journals The Influence of ProbioticLactobacillus caseiin Combination with Prebiotic Inulin on the Antioxidant Capacity of Human Plasma

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Paulina Kleniewska ◽  
Arkadiusz Hoffmann ◽  
Ewa Pniewska ◽  
Rafał Pawliczak
Keyword(s):  
Antioxidant Capacity ◽  
Human Plasma ◽  
Antioxidant Properties ◽  
Positive Influence ◽  
Reducing Ability ◽  
Plasma Antioxidant Capacity ◽  
Synbiotic Supplementation ◽  
Plasma Antioxidant ◽  
And Control ◽  
Gpx Activity

The aim of the present study was to assess whether probiotic bacteriaLactobacillus casei(4 × 108 CFU) influences the antioxidant properties of human plasma when combined with prebiotic Inulin (400 mg). Experiments were carried out on healthy volunteers (n=32). Volunteers were divided according to sex (16 male and 16 female) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 weeks, at the end of the study. Catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activity, and the ferric reducing ability of plasma (FRAP) in human plasma were examined. The administration of synbiotics containingL. caseiplus Inulin resulted in a significant increase in FRAP values (p=0.00008) and CAT activity (p=0.02) and an insignificant increase in SOD and GPx activity compared to controls. Synbiotics containingL. casei(4 × 108 CFU) with prebiotic Inulin (400 mg) may have a positive influence on human plasma antioxidant capacity and the activity of selected antioxidant enzymes.

scholarly journals Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 992 ◽  
Author(s):  
Joanna Pokorska ◽  
Dominika Kułaj ◽  
Agata Piestrzyńska-Kajtoch ◽  
Anna Radko
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Lipocalin 2 ◽  
Holstein Friesian ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Daily Milk Yield ◽  
Friesian Cows

In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.

scholarly journals Relationship between vitamin intake and total antioxidant capacity in elderly adults

2016 ◽  
Vol 21 (2) ◽  
pp. 167 ◽  
Author(s):  
Miriam Lucia Ojeda Arredondo ◽  
Magda Catalina Pinilla Betancourt ◽  
Martha Lucia Borrero Yoshida ◽  
Gonzalo Sequeda ◽  
Vivian Maritza Castro Herrera ◽  
...  
Keyword(s):  
Vitamin A ◽  
Antioxidant Capacity ◽  
Fruits And Vegetables ◽  
Natural Antioxidants ◽  
Absorption Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
P Value ◽  
Food Composition Tables ◽  
Plasma Antioxidant Capacity ◽  
Plasma Antioxidant

<p>The consumption of foods high in natural antioxidants, like fruits and vegetables, is associated with a lower risk of oxidative stress-related diseases. The aim of this study was to establish the relationship between the plasma antioxidant capacity in adults over fifty and their intake of vitamin A, C, and E. We evaluated 118 24-hour recalls of intake of foods. The intake of vitamin A, C, and E was quantified using food composition tables. We quantified plasma phenols using the Folin-Ciocalteu method. The antioxidant capacity was determined using the Trolox Equivalent Antioxidant Capacity (TEAC) and Oxygen Radical Absorption Capacity (ORAC) methods. Correlation analyses were performed between the studied variables and a positive correlation was found in most cases. However, none of the correlations was statistically significant. In all cases p-value was &gt;0.05. The quantification of nutrient intake is not an adequate predictor of plasma antioxidant capacity in individuals over fifty</p>

Effect of Different Drying Processes on the Physicochemical and Antioxidant Properties of Thinned Young Apple

2015 ◽  
Vol 11 (2) ◽  
pp. 207-219 ◽  
Author(s):  
Weiqi Chen ◽  
Yurong Guo ◽  
Juan Zhang ◽  
Xiaorui Zhang ◽  
Yonghong Meng
Keyword(s):  
Antioxidant Capacity ◽  
Large Scale ◽  
Antioxidant Properties ◽  
Food Resource ◽  
Reducing Power ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Scale Production ◽  
Large Scale Production ◽  
Trolox Equivalent ◽  
Dpph Scavenging

Abstract In China, there are about 1.6 million tons of thinned young apples (TYAs) every year after blossoming and fruit thinning. These resulting fruits, in reality, could be utilized as agricultural and food resource. This study was undertaken to evaluate the influence on physical properties, bioactive attributes of TYAs that were subjected to chosen drying techniques. In general, significant differences (p < 0.05) exist in physical appearance and polyphenol. Antioxidant capacity was also investigated on the basis of assay for Trolox equivalent antioxidant capacity (TEAC) by 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), reducing power and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability. Judging from the results, it was concluded that hot air drying at 60℃ is appropriate for large-scale production; furthermore, vacuum freeze drying tends to manufacture high-value up-market TYA products that are available to give the best results for dehydrated fruits. It is suggested that TYA has great potential in the food industry as functional ingredient.

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