Biological efficacy of Trichoderma harzianum isolate to control some fungal pathogens of wheat (Triticum aestivum) in Turkey

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Çiğdem Küçük ◽  
Merih Kivanç ◽  
Engin Kinaci ◽  
Gülcan Kinaci
Keyword(s):  
Trichoderma Harzianum ◽  
Yield Components ◽  
Fungal Pathogens ◽  
Fusarium Culmorum ◽  
Considerable Effect ◽  
Biocontrol Agents ◽  
Gaeumannomyces Graminis ◽  
Microbial Number ◽  
Effect On Yield ◽  
Seed Application

AbstractGaeumannomyces graminis var. tritici, Fusarium culmorum and F. moniliforme are highly important and widespread pathogens of wheat in Turkey. Trichoderma isolates have been used as biocontrol agents to protect plants against soilborne diseases in several crops. The present work was carried out to evaluate the potential of Trichoderma harzianum isolate T1 as biocontrol agents for G. graminis, F. culmorum and F. moniliforme under field conditions in 2001 and 2002. Quantitative differences were found in microbial number in soil. T. harzianum T1 had considerable effect on population densities of the tested pathogens. The total number of G. graminis, F. culmorum and F. moniliforme were lower in the T. harzianum T1 application made to seed. T. harzianum T1 application to seed had increasing affect on the yield components of wheat through better control over pathogens. The greatest counts of T. harzianum T1 were detected on root segments. Seed application by T. harzianum T1 had increasing effect on yield components of wheat.

scholarly journals Biocontrol agents of sclerotial blight in tea

2013 ◽  
Vol 3 (1) ◽  
pp. 54-56
Author(s):  
Indramani Bhagat ◽  
Bishwanath Chakraborty
Keyword(s):  
Trichoderma Harzianum ◽  
Fungal Pathogens ◽  
Sclerotium Rolfsii ◽  
Biocontrol Agents ◽  
Seedling Blight ◽  
Blight Disease ◽  
In Vitro Interaction

One of the important fungal pathogens Sclerotium rolfsii, causing seedling blight disease in tea was found to be predominant in the nursery grown plants. In vitro interaction of S. rolfsii with Trichoderma harzianum and T. viride was studied. Both bioagents inhibited the growth of S. rolfsii.

scholarly journals The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 349
Author(s):  
Dominik Bleša ◽  
Pavel Matušinský ◽  
Romana Sedmíková ◽  
Milan Baláž
Keyword(s):  
Biological Control ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Higher Plants ◽  
Fusarium Culmorum ◽  
Suppressive Effect ◽  
Plant Production ◽  
Shoot Biomass ◽  
Organic Substrates

The use of biological control is becoming a common practice in plant production. One overlooked group of organisms potentially suitable for biological control are Rhizoctonia-like (Rh-like) fungi. Some of them are capable of forming endophytic associations with a large group of higher plants as well as mycorrhizal symbioses. Various benefits of endophytic associations were proved, including amelioration of devastating effects of pathogens such as Fusarium culmorum. The advantage of Rh-like endophytes over strictly biotrophic mycorrhizal organisms is the possibility of their cultivation on organic substrates, which makes their use more suitable for production. We focused on abilities of five Rh-like fungi isolated from orchid mycorrhizas, endophytic fungi Serendipita indica, Microdochium bolleyi and pathogenic Ceratobasidium cereale to inhibit the growth of pathogenic F. culmorum or Pyrenophora teres in vitro. We also analysed their suppressive effect on wheat infection by F. culmorum in a growth chamber, as well as an effect on barley under field conditions. Some of the Rh-like fungi affected the growth of plant pathogens in vitro, then the interaction with plants was tested. Beneficial effect was especially noted in the pot experiments, where wheat plants were negatively influenced by F. culmorum. Inoculation with S. indica caused higher dry shoot biomass in comparison to plants treated with fungicide. Prospective for future work are the effects of these endophytes on plant signalling pathways, factors affecting the level of colonization and surviving of infectious particles.

scholarly journals Evaluation of nitrogen (Urea) on Zea mays L. under Balkh province climate

2020 ◽  
Vol 3 (1) ◽  
pp. 6-9
Author(s):  
Nasratullah Habibi ◽  
Friba Sikandari
Keyword(s):  
Zea Mays ◽  
Nitrogen Fertilizer ◽  
Yield Components ◽  
Zea Mays L ◽  
Agricultural Research ◽  
Block Design ◽  
High Yield ◽  
Randomized Complete Block Design ◽  
Yield And Yield Components ◽  
Effect On Yield

An experiment was conducted to evaluate effect of urea fertilizer on yield and yield components of Zea mays L. Using Randomized Complete Block Design (RCBD) split plots in three replications in agricultural research farm of Balkh University by 2019. Doses of urea used in this experiment were 0, 80, 160 and 240 kg ha-1 , respectively. It has been found that amount of nitrogen fertilizer (urea) had significant effect on yield of maize at p<0.05. The higher level of nitrogen caused the higher grain yield, number of kernels per ear, the number of grains per ear row, ear diameter, cob length, grain per plant and plant height. As a result 7.76 ton ha-1 was recorded as high yield while 240 kg ha-1 urea was used, and 5.12 ton ha-1 was recorded as low yield in treatment one with 0 kg ha-1 of nitrogen fertilizer. Finally, as a result using 240 kg ha-1 nitrogen fertilizer is recommended.

Finding new QTL for yield traits based on a high-density genetic map in the super hybrid rice Nei2You No.6

2019 ◽  
Author(s):  
Miao Zhang ◽  
Zhengping Zhou ◽  
Yuyu Chen ◽  
Yongrun Cao ◽  
Chenwei Deng ◽  
...  
Keyword(s):  
Complex Traits ◽  
Yield Components ◽  
Average Density ◽  
High Density ◽  
Yield Traits ◽  
Target Region ◽  
Effect On Yield ◽  
Positive Effect ◽  
High Density Linkage Map ◽  
1000 Grain Weight

Abstract Background Rice is one of the most important food crops in the world. To determine the genetic basis of yield components in super rice Nei2You No.6, 387 recombinant inbred sister lines (RISLs) were obtained for mapping quantitative trait loci (QTL) responsible for yield-associated traits, such as 1000-grain weight (TGW), grain number per plant (GNP), number of panicles per plant (NP), and grain yield per plant (GYP). Results Using whole genome re-sequencing, a high-density linkage map consisting of 3203 bin markers was constructed with total genetic coverage of 1951.1 cM and an average density of 0.61 cM. As a result of the multi-environment test, 43 yield-related QTL were mapped to all 12 chromosomes, among which 28 inherited from Nei2B showed a positive effect on yield traits. Nine QTL, qTGW-1a, qTGW-5, qTGW-7, qTGW-10b, qTGW-10c, qTGW-12, qNP-7, qGNP-6c, and qGYP-6b, showed stable effects across multiple environments. Five of the nine QTL were co-located with previously reported QTL, and four novel loci, qTGW-7, qTGW-12, qGNP-6c, and qNP-7, were identified in the present study. Subsequently, qNP-7, qTGW-12, and qTGW-7 were validated using corresponding paired lines which differed only in the target region. Conclusions the RISL population is an effective tool for mapping and validating QTL of complex traits, for instance, yield-associated traits, and newly detected QTL provide new genetic resources for research of yield components and molecular breeding in rice.

scholarly journals Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 189
Author(s):  
Omar A. Hewedy ◽  
Khalid S. Abdel Lateif ◽  
Mahmoud F. Seleiman ◽  
Ashwag Shami ◽  
Fawziah M. Albarakaty ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Phosphate Solubilization ◽  
Random Amplified Polymorphic Dna ◽  
Trichoderma Viride ◽  
Biocontrol Agents ◽  
Agricultural System ◽  
Dual Culture ◽  
In Planta ◽  
Trichoderma Species ◽  
Soil Borne Pathogens

Trichoderma species are known as excellent biocontrol agents against soil-borne pathogens that cause considerable crop losses. Eight strains of Trichoderma were isolated from five Egyptian regions. They identified based on translation elongation factor-1α (TEF1) sequencing as four different Trichoderma species: Trichoderma asperellum, Trichoderma harzianum, Trichoderma viride, and Trichoderma longibrachiatum. Optimal growth conditions (temperature and media), and the phosphate solubilization capability of Trichoderma strains were evaluated in vitro. Further, the ability of these strains to antagonize Fusarium solani, Macrophomina phaseolina, and Fusarium graminearum was also evaluated. The results revealed that Trichoderma harzianum (Th6) exhibited the highest antagonistic ability against F. solani, M. phaseolina and F. graminearum with inhibition rates of 71.42%, 72.97%, and 84.61%, respectively. Trichoderma viride (Tv8) exhibited the lowest antagonism against the same pathogens with inhibition rates of 50%, 64% and 69.23%, respectively. Simple-sequence repeats (SSRs) and random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic variability of the Trichoderma strains. The results revealed that of 45 RAPD amplified bands, 36 bands (80%) were polymorphic and of SSRs amplified 36 bands, 31 bands (86.11%) were polymorphic. The amplification of calmodulin and β-1,3-endoglucanase was noted at 500 bp and 230 bp, respectively. Data indicated that T. viride (Tv8) had the highest phosphate solubilization index (10.0 mm), while T. harzianum (Th6) had the lowest phosphate solubilization index (4.0 mm). In conclusion, T. harzianum (Th6) had the highest antagonistic activity in dual culture assay along with the growth rate; while T. viride (Tv8) had the highest phosphate solubilization activity. There are still gaps in obtaining new formulations, selecting potent Trichoderma strains to confirm disease control in planta. For improving Trichoderma recommendation in the organic agricultural system and sustaining the fertility of the soil, the field application of highly antagonistic biocontrol agents in different types of soil and plant species will be the first approach toward bio-pesticide treatments along with bio-fertilizer inoculation. Furthermore, secondary metabolites will be investigated for the most promising strains with the combination of different pathogens and application timing.

scholarly journals Pseudomonas synxantha 2-79 Transformed with Pyrrolnitrin Biosynthesis Genes Has Improved Biocontrol Activity Against Soilborne Pathogens of Wheat and Canola

2020 ◽  
Vol 110 (5) ◽  
pp. 1010-1017
Author(s):  
Jibin Zhang ◽  
Dmitri V. Mavrodi ◽  
Mingming Yang ◽  
Linda S. Thomashow ◽  
Olga V. Mavrodi ◽  
...  
Keyword(s):  
Root Rot ◽  
Fusarium Culmorum ◽  
Pythium Ultimum ◽  
Gaeumannomyces Graminis ◽  
Wild Type ◽  
Wheat Roots ◽  
Recombinant Strains ◽  
Rhizoctonia Root Rot ◽  
Take All

A four-gene operon (prnABCD) from Pseudomonas protegens Pf-5 encoding the biosynthesis of the antibiotic pyrronitrin was introduced into P. synxantha (formerly P. fluorescens) 2-79, an aggressive root colonizer of both dryland and irrigated wheat roots that naturally produces the antibiotic phenazine-1-carboxylic acid and suppresses both take-all and Rhizoctonia root rot of wheat. Recombinant strains ZHW15 and ZHW25 produced both antibiotics and maintained population sizes in the rhizosphere of wheat that were comparable to those of strain 2-79. The recombinant strains inhibited in vitro the wheat pathogens Rhizoctonia solani anastomosis group 8 (AG-8) and AG-2-1, Gaeumannomyces graminis var. tritici, Sclerotinia sclerotiorum, Fusarium culmorum, and F. pseudograminearum significantly more than did strain 2-79. Both the wild-type and recombinant strains were equally inhibitory of Pythium ultimum. When applied as a seed treatment, the recombinant strains suppressed take-all, Rhizoctonia root rot of wheat, and Rhizoctonia root and stem rot of canola significantly better than did wild-type strain 2-79.

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