scholarly journals Cutaneous Blastomycosis – a case report

2012 ◽  
Vol 4 (3) ◽  
pp. 119-128
Author(s):  
Đuka Ninković Baroš ◽  
Jagoda Balaban ◽  
Svetlana Tomašević Pavlović ◽  
Aleksandra Salapura Dugonjić ◽  
Gorana Popović ◽  
...  
Keyword(s):  
Bosnia And Herzegovina ◽  
Periodic Acid ◽  
Great Majority ◽  
Surgical Excision ◽  
Skin Lesions ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Dimorphic Fungi ◽  
Negative Woman ◽  
Banja Luka

Abstract We present a 46-year-old non-atopic HIV-negative woman from Doboj, Republic of Srpska, Bosnia and Herzegovina, who was referred to the Department of Dermatovenereology, Clinical Center Banja Luka, Republic of Srpska, Bosnia and Herzegovina, with a 3-month long history of an erythematous, large indurated infl amed area on the upper arm. The condition was asymptomatic, immediately following surgical excision of a small tumor. After exlusion of pulmonary blastomycosis and other organ involvement, the diagnosis of primary inoculation cutaneous blastomycosis was made based on clinical presentation and histopathological fi ndings. Histopathology revealed thick-walled, rounded, budding yeasts with broad-based buds that stained pink with periodic acid-schiff (PAS) staining. Itraconazole therapy was initiated at a dose of 2x100 mg/day. After three months of therapy, the dose of itraconazole was increased to 2x200 mg/day during the next three months, and then the dose was reduced to 2x100 mg. Blastomycosis is an uncommon, chronic granulomatous and suppurative mycosis caused by Blastomyces dermatitidis, which belongs to the group of main endemic systemic mycoses and in the great majority of cases represents a primary pulmonary disease. Few sporadic cases have been reported in Europe. There are three forms of blastomycosis: primary cutaneous, pulmonary and disseminated. B. dermatitidis has rarely been isolated from the environment. Wood debris or land close to rivers or subject to fl ooding are considered to be the natural substrate. The fungus can grow in sterile soil in the laboratory, and it is believed that humans get infected by inhaling spores from a saprophytic source. Primary cutaneous blastomycosis is very rare and it is found in farmers and laboratory workers. Human to human transmission does not normally occur. The diagnosis of the skin lesions is made by direct microscopy of skin samples (e.g., pus, scrapings) with 10% potassium hydroxide mount and confi rmed by culture or biopsy. Histopathological analysis provides identifi cation of all the dimorphic fungi. However, this can be complicated by the fact that in some cases they can be morphologically atypical or sterile. In the tissues, B. dermatitidis produces characteristic thick-walled, rounded, refractile, and spherical budding yeasts with broad-based buds. Of the available antimycotic drugs, itraconazole 200 mg/day is probably the most effective, but at least 400 mg/day is redommended initially.

scholarly journals The role of histopathology in the diagnosis of dermatophytoses / Značaj patohistološkog nalaza u dijagnostici dermatofitoza

2010 ◽  
Vol 2 (2) ◽  
pp. 45-53 ◽  
Author(s):  
Đorđi Gocev ◽  
Katerina Damevska
Keyword(s):  
Fungal Infection ◽  
Periodic Acid ◽  
False Negative ◽  
Histopathological Analysis ◽  
Unexpected Finding ◽  
Skin Infections ◽  
Specific Staining ◽  
Periodic Acid Schiff ◽  
Skin Reactions ◽  
Pas Staining

Abstract Histopathological analysis is not a routine procedure for diagnosing fungal skin infections. In the histopathological specimens, fungi are visible only when using special stain such as periodic acid-Schiff (PAS). However, histopathological analysis may not be performed in small laboratories. Histopathological characteristics of fungal skin infections are not specific. In all skin biopsy cases, obtained without clinical suspicion of fungal infection, the knowledge of certain, most frequent histopathological reaction patterns, as well as specific histopathological indicators (a diagnostic histopathological “clue”), of certain superficial mycoses e.g., dermatophytoses, may raise a suspicion of fungal infection and warrant a fungal-specific staining. A retrospective analysis of all PAS-stained sections was carried out. All PAS-positive biopsy specimens were assessed for clinical features, histopathological patterns of skin reactions, and presence of histopathological indicators. Our results have shown that out of the total of 361 PAS-stained sections, fungal hyphae were identified in 12 (3.3%) specimens. In 5 (1.4%) cases, the diagnosis of fungal infection was suspected on clinical grounds, while in 7 (1.9%) cases detection of fungi was an unexpected finding. The most frequent type of histopathological pattern was spongiotic, and the most frequent histopathological indicator was the presence of neutrophils within the epidermis. Our results confirm that dermatophytoses may present with clinical and histological non-specific findings. PAS staining represents a relatively cheap and simple fungal-specific staining. It has been suggested that it not only confirms that the selected material is actually invaded, but also reduces the number of false-negative direct reports, where fungi are cultured from a microscopically negative specimen. Apart from a small percentage of positive findings, our results justify the need for routine PAS staining of all clinically and histologically non-specific inflammatory skin conditions.

scholarly journals Psoriasis-Like Inflammation Induced Renal Dysfunction through the TLR/NF-κB Signal Pathway

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Fang Ren ◽  
Min Zhang ◽  
Caiyun Zhang ◽  
Hong Sang
Keyword(s):  
Renal Injury ◽  
Periodic Acid ◽  
Severity Index ◽  
Skin Lesions ◽  
Signal Pathway ◽  
Inflammatory Factors ◽  
Urine Protein ◽  
Periodic Acid Schiff ◽  
Associated Proteins ◽  
Pas Staining

Pathological studies have shown an association between psoriasis and renal injury (RI), but the mechanism between RI and psoriasis was still unclear. This paper was designed to investigate the relationship and mechanism between psoriasis-like inflammation and renal injury in BALB/C mice. Mice were topically smeared imiquimod followed by various analyses in skin lesions, urine protein, kidney/serum inflammatory cytokines, kidney function, podocyte membrane proteins, and toll-like receptors/nuclear factor kappa-b (TLR/NF-κB) pathway-associated proteins. Meanwhile, lipopolysaccharide (LPS) and dexamethasone (DEX) were intraperitoneally injected to promote and inhibit inflammation accompanied by imiquimod to elaborate the relevance between inflammatory levels and RI. In the model group, the Psoriasis Area and Severity Index (PASI) scores of scaly and erythema obviously increased (p<0.01), creatinine and blood urea nitrogen significantly increased (p<0.01), the positive area of hematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining in kidney increased (p<0.01), malondialdehyde significantly increased with superoxide dismutase (SOD) decreased (p<0.01), 24-hour urine protein increased and the expressions of podocin and CD2 associate protein (CD2AP) decreased (p<0.01), and kidney/serum inflammatory factors (IL-17, IL-1β, IL-6, TNF-α, and IL-22) and TLR/NF-κB-related expression (TLR2, TLR4, MyD88, and NF-κBp65) all increased (p<0.01). The RI was aggravated with the TLR/NF-κB related expression being upregulated by LPS (p<0.05). On the contrary, the RI was alleviated by DEX (p<0.05). Our data showed that psoriasis-like inflammation damaged the renal function via the TLR/NF-κB signal pathway. Inhibiting TLR/NF-κB-related protein expression may be effective for the treatment of RI caused by psoriasis.

scholarly journals Gastric B-Cell Lymphoma with Mott Cell Differentiation in a Dog

2009 ◽  
Vol 21 (5) ◽  
pp. 715-719 ◽  
Author(s):  
Gabrita De Zan ◽  
Valentina Zappulli ◽  
Laura Cavicchioli ◽  
Linda Di Martino ◽  
Eriberta Ros ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
Gastrointestinal Disease ◽  
Periodic Acid ◽  
Palliative Therapy ◽  
Clinical Signs ◽  
B Cell Lymphoma ◽  
Surgical Excision ◽  
Periodic Acid Schiff ◽  
Regional Lymph Nodes

A gastric lymphoid tumor with involvement of regional lymph nodes and spleen was diagnosed in an 8-year-old crossbreed male dog with a 6-month history of gastrointestinal disease. Despite surgical excision and palliative therapy (prednisolone and cimetidine), the dog was euthanized due to worsening of clinical signs. At necropsy, multiple white, solid, nodular, infiltrative masses were observed in the stomach, duodenum, spleen, liver, and lungs in association with generalized lymph node enlargement. Cytology, histology, histochemistry, immunohistochemistry, and electron microscopy revealed that the neoplastic cell population was composed of B lymphocytes that contained variable amounts of round periodic acid-Schiff-positive cytoplasmic globules consistent with Russell bodies. The tumor most likely represented a variant of B-cell neoplasia with extensive Mott cell differentiation.

scholarly journals A rare case of perianal apocrine adenocarcinoma

2020 ◽  
Vol 2020 (11) ◽  
Author(s):  
Victor Gazivoda ◽  
Lawrence P Kiss ◽  
Rebecca Rhee
Keyword(s):  
Clinical Presentation ◽  
Periodic Acid ◽  
Surgical Excision ◽  
Cytokeratin 7 ◽  
Perianal Region ◽  
Periodic Acid Schiff ◽  
High Incidence ◽  
Apocrine Adenocarcinoma ◽  
Mitotic Figures ◽  
Cystic Disease Fluid Protein

Abstract Apocrine adenocarcinoma is a rare primary cutaneous malignancy that arises from areas with high apocrine gland density, most frequently described in the axilla. There have only been three previously reported cases of apocrine adenocarcinoma in the anal/perianal region. A 72-year-old female presented for evaluation of a perianal lesion with persistent drainage that she had noticed for over a year. The patient proceeded with surgical excision of the perianal nodule. Diagnosis was made based on pathology demonstrating areas of mixed solid and trabecular areas with large nuclei and many prominent mitotic figures, which stained positive for periodic acid–Schiff–diastase, cytokeratin 7 and gross cystic disease fluid protein 15. We are reporting just the fourth such case of apocrine adenocarcinoma in the anal/perianal region. It is important to consider apocrine adenocarcinoma in our differential, because though apocrine adenocarcinoma has a benign clinical presentation, it can have a high incidence of lymph invasion on presentation.

scholarly journals Concomitant fungal and Mycobacterium bovis infections in beef cattle in Kenya

2013 ◽  
Vol 80 (1) ◽  
Author(s):  
Joseph N. Kuria ◽  
Stephen M. Gathogo
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Present Report ◽  
Paracoccidioides Brasiliensis ◽  
Periodic Acid ◽  
Endemic Region ◽  
Periodic Acid Schiff ◽  
Dimorphic Fungi ◽  
Tissue Sections ◽  
Fast Procedure

Bovine tuberculosis is an important zoonosis and accurate diagnosis is important for its surveillance. Post-mortem diagnosis may, however, be compromised by lesions caused by other pathogens. In an investigation on its prevalence in slaughter cattle in Kenya, Mycobacterium bovis and dimorphic fungi were inadvertently identified separately or concurrently in tuberculous lesions. Beef carcasses were inspected for lesions in two abattoirs in Nairobi. Tissues with lesions were collected and transported to the laboratory. Smears of lesions were stained by acid-fast procedure and examined microscopically. Lesions were cultured in Löwenstein-Jensen (LJ) and in BBL TM Mycobacterium growth indicator tubes (MGIT) media. Mycobacteria isolates in LJ medium were identified by DNA typing. Smears of BBLTM MGIT cultures were acid-fast stained and examined microscopically. Tissue sections were stained with periodic acid-Schiff reagent before examination. Of the 929 carcasses examined, 176 had granulomatous lesions. Dimorphic fungi were detected as acid-fast negative cells in 58 (32.9; 33.5%) of the lesion smears, either alone (29.0; 16.4%) or concurrently with acid-fast bacilli (29.0; 16.4%). The fungi were also detected in some BBL TM MGIT-culturesmears and lesioned tissue sections. The fungi were identified, by means of cellular morphology, as Paracoccidioides brasiliensis and Blastomyces dermatitidis. A total of 64 isolates of mycobacteria were recovered in LJ medium, 19 of which were identified as M. bovis. The present report documents native P. brasiliensis infections outside the presumed endemic region and B. dermatitidis infections in a livestock animal. The findings further indicate the importance of dimorphic fungi as a differential diagnosis of bovine tuberculosis in the region.

Hepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study

2021 ◽  
Author(s):  
Zuleyha Erisgin ◽  
Hasan Serdar Mutlu ◽  
Yavuz Tekelioglu ◽  
Engin Deveci ◽  
Ugur Seker
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Periodic Acid ◽  
Flow Cytometric Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Flow Cytometric ◽  
Transmission Electron

Abstract This study aims to investigate the effects of melamine exposure from the weaning period (21st postnatal days in rats) on liver tissue. Female Wistar albino rats (n = 18) were divided into three groups. About 0.1-ml saline was applied to the control group by gavage for 21 days from the postnatal 21st day. The second group was taken 50-mg/kg melamine (in 0.1-ml saline) and the third group was taken 75-mg/kg melamine (in 0.1-ml saline) p.o. On the postnatal 45th day, all rats were sacrificed under anesthesia. Then, liver tissues were cut into three parts and two of them placed in neutral formalin for histopathological and flow cytometric analysis, and one of them placed in 2.5% glutaraldehyde. Histopathological analysis was performed with hematoxylin & eosin, Masson trichrome, periodic acid Schiff stained sections, and also with transmission electron microscopy. Apoptosis (Annexin V positivity) was analyzed by flow cytometry. According to histopathological analysis, hepatocyte damage, sinusoidal dilatation, and inflammatory cell infiltration significantly increased in both melamine groups compared with the control group. Apoptosis significantly increased in the 50 and 75-mg melamine groups compared with the control group. In the results of transmission electron microscopy analysis, there was abnormal chromatin distribution in the hepatocyte nuclei, loss in the cristae of the mitochondria, and organelle loss in large areas in the cytoplasm in both melamine exposure groups. As result, melamine exposure from the weaning period causes liver damage with increasing doses.

Pyogenic granuloma associated with conjunctival epithelial neoplasia: report of nine cases

2019 ◽  
Vol 103 (10) ◽  
pp. 1469-1474 ◽  
Author(s):  
Martina C Herwig-Carl ◽  
Hans E Grossniklaus ◽  
Philipp L Müller ◽  
Lisa Atzrodt ◽  
Karin U Loeffler ◽  
...  
Keyword(s):  
Demographic Data ◽  
Periodic Acid ◽  
Case Series ◽  
Pyogenic Granuloma ◽  
R0 Resection ◽  
Surgical Removal ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Clinical Appearance ◽  
Complete Surgical Excision

AimsTo systematically describe the clinical and histopathological features of a case series of conjunctival carcinomatous lesions underlying as—and also masquerading—pyogenic granuloma.MethodsNine cases of conjunctival carcinomatous lesions underlying a pyogenic granuloma (which were clinically predominant) were retrospectively identified. Patients’ records were analysed for demographic data, clinical appearance and the postoperative course. Formalin-fixed paraffin-embedded specimens were routinely processed and stained with H&E and periodic acid-Schiff. Immunohistochemical stains for cytokeratin were performed in selected cases.ResultsAll nine tumours were located in the conjunctiva (bulbar, tarsal, limbal conjunctiva) of patients between 44 and 80 years. The lesions exhibited clinical features of pyogenic granuloma which dominated the clinical appearance. Additional features comprised a papillomatous appearance of the adjacent conjunctiva, a more whitish aspect of the lesion and a history of squamous cell carcinoma (SCC) respectively surgery for other entities. Histopathological analysis revealed a carcinomatous lesion (conjunctival intraepithelial neoplasia or SCC) at the base of a classic pyogenic granuloma in all nine cases. Surgical removal (R0 resection) was performed. Three cases received adjuvant mitomycin C or interferon α2b treatment. Two lesions locally recurred within 2 years after initial presentation.ConclusionCarcinomatous lesions may be accompanied by a pyogenic granuloma which may dominate the clinical pictures. As the tumour is usually located at the base of the lesion, a complete surgical excision followed by histopathological analysis is mandatory for each lesion appearing as conjunctival pyogenic granuloma.

scholarly journals Novel inflammatory cell infiltration scoring system to investigate healthy and footrot affected ovine interdigital skin

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5097 ◽  
Author(s):  
Michael Agbaje ◽  
Catrin S. Rutland ◽  
Grazieli Maboni ◽  
Adam Blanchard ◽  
Melissa Bexon ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Scoring System ◽  
Inflammatory Cell ◽  
Periodic Acid ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Histopathological Analysis ◽  
Periodic Acid Schiff ◽  
Dichelobacter Nodosus ◽  
Inflammatory Score

Ovine footrot is a degenerative disease of sheep feet leading to the separation of hoof-horn from the underlying skin and lameness. This study quantitatively examined histological features of the ovine interdigital skin as well as their relationship with pro-inflammatory cytokine (IL-1β) and virulent Dichelobacter nodosus in footrot. From 55 healthy and 30 footrot ovine feet, parallel biopsies (one fixed for histology) were collected post-slaughter and analysed for lesions and histopathological analysis using haematoxylin and eosin and Periodic Acid-Schiff. Histological lesions were similar in both conditions while inflammatory scores mirror IL-1β expression levels. Increased inflammatory score corresponded with high virulent D. nodosus load and was significant (p < 0.0001) in footrot feet with an inflammatory score of 3 compared to scores 1 and 2. In addition, in contrast to healthy tissues, localisation of eubacterial load extended beyond follicular depths in footrot samples. The novel inflammatory cell infiltration scoring system in this study may be used to grade inflammatory response in the ovine feet and demonstrated an association between severity of inflammatory response and increased virulent D. nodosus load.

scholarly journals Cystadenocarcinoma simulating hemangiosarcoma of the salivary gland in dog: case report

2011 ◽  
Vol 63 (3) ◽  
pp. 574-578
Author(s):  
C.O. Gamba ◽  
K.A. Damasceno ◽  
C.B. Campos ◽  
E. Ferreira ◽  
G.D. Cassali
Keyword(s):  
Salivary Gland ◽  
Blood Cells ◽  
Periodic Acid ◽  
Morphological Characteristics ◽  
Immunohistochemical Analysis ◽  
Histopathological Analysis ◽  
High Grade ◽  
Periodic Acid Schiff ◽  
Tumor Mass ◽  
Pas Staining

Cystadenocarcinoma is regarded as a rare adenocarcinoma variant in animals. This work reports the case of an 8-year-old female Poodle dog with salivary gland cystadenocarcinoma with morphological characteristics similar to a hemangiosarcoma. Histopathological analysis showed a tumor mass with cystic formations containing a large amount of red blood cells. In order to distinguish these two entities, periodic acid Schiff (PAS) staining and immunohistochemical analysis were carried out with the use of cytokeratin AE1/AE3 (CK) and CD31-specific antibodies. Neoplastic cells were PAS-negative, CK-positive and CD31-negative confirming their epithelial origin. Based on the findings, the diagnosis of high grade cystadenocarcinoma was established.

scholarly journals Lymphocystic Disease in Wild Croaker (Micropogonias furnieri) and Flounder (Paralichthys orbignyanus)

2020 ◽  
pp. 29-36
Author(s):  
V. F. Pedrosa ◽  
M. C. Klosterhoff ◽  
A. F. F. De Medeiros ◽  
C. A. Paz-Villarraga ◽  
L. A. Romano
Keyword(s):  
Periodic Acid ◽  
Skin Lesions ◽  
Periodic Acid Schiff ◽  
Lymphocystis Disease ◽  
In The Wild ◽  
Macroscopic Findings ◽  
Hematoxylin And Eosin ◽  
Micropogonias Furnieri ◽  
The City ◽  
Paralichthys Orbignyanus

Lymphocystis disease has been reported worldwide in several species of freshwater fish and marine fish, naturally infected in the wild environment, or in intensive crops in farms. Nodular warty lesions of irregular surface were observed in the tegument and fins and mouth in a species of croaker (Micropogonias furnieri) caught in Cassino beach Rio Grande RS, Brazil and flounder (Paralichthys orbignyanus) caught in the city of San Clemente, Argentina. The skin lesions fragments were fixed in 20% buffered formalin, and the histological sections were stained with hematoxylin and eosin and Periodic Acid-Schiff (PAS), where microscopic alterations were visualized in the form of hyaline capsule with small basophilic structures in nodules and fibroblastic cells proliferation. The reported cases were based on the disease macroscopic findings characteristic of a lymphocystis disease, along with the histopathology, which confirmed the presence of the disease in the analyzed tissues.

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