scholarly journals Effects of Anti-Ethylene Treatments on Ethylene Production and Antioxidant Activities in Cut Spray Carnation

2012 ◽  
Vol 20 (2) ◽  
pp. 173-182
Author(s):  
Mahnaz Karimi ◽  
Moazzam Hassanpour Asil ◽  
Ghorbanali Nematzadeh ◽  
Hedayat Zakizadeh
Keyword(s):  
Antioxidant Enzymes ◽  
Ethylene Production ◽  
Enzyme Activities ◽  
Antioxidant Activities ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Cut Flowers ◽  
Acc Content ◽  
Oxyacetic Acid

Abstract The present investigation was aimed to study changes in ethylene production, ACC content and antioxidant enzymes of cut spray carnation (Dianthus caryophyllus L.) flowers that had been treated with amino-oxyacetic acid (AOA), benzyladenine (BA) and 1-methylcyclopropene (1-MCP). Maximum vase life in ‘Optima’ spray carnations was obtained with 0.6 mg l-1 1-MCP. Ethylene production was significantly decreased by AOA at concentrations over 100 mg l-1, BA at 30 mg l-1, and 1-MCP at all concentrations, compared with the control. A significant increase in ACC content was observed in 1-MCP treated cut flowers compared with the control. However, the decline in ACC content was observed after using 100 or 150 mg l-1 AOA. A significant increase in SOD, CAT and POX enzyme activities was observed in the treatment with 0.6 mg l-1 1-MCP.

scholarly journals 24-Epibrasinolide Modulates the Vase Life of Lisianthus Cut Flowers by Modulating ACC Oxidase Enzyme Activity and Physiological Responses

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 995
Author(s):  
Mohammad Darvish ◽  
Habib Shirzad ◽  
Mohammadreza Asghari ◽  
Parviz Noruzi ◽  
Abolfazl Alirezalu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Water Absorption ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Postharvest Quality ◽  
Vase Life ◽  
Dependent Manner ◽  
Cut Flowers ◽  
Oxidase Enzyme

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.

scholarly journals Prolonging the vase life of cut Carnation 'GIOKO' by using different chemicals

2008 ◽  
Vol 14 (4) ◽  
Author(s):  
O. Terék ◽  
F. A. S. Hassan ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Untreated Control ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Distilled Water ◽  
Cut Flowers ◽  
Chemical Treatments ◽  
All Solutions ◽  
Separate Treatment ◽  
Flower Diameter ◽  
Positive Effect

Cut flowers of Dianthus caryophyllus L. cv. GIOKO were treated with different concentrations of sucrose and in combination with 1­methylcyclopropene (1-MCP) to compare the effect of these treatments with floral preservative (`Spring') on the longevity of flowers. Distilled water was used for preparing all solutions. The control flowers were held in distilled water. Clorox at 2 mL- I was added to all treatments containing sucrose and it was also applied as a separate treatment. The vase life of cut carnations was significantly prolonged due to the use of chemical treatments, as compared to the untreated control. The longest vase life (18.33 days) was obtained by using 1-MCP 0.5 g m-3 for 6 h treatment. All concentrations of sucrose had a positive effect on flower diameter. The best treatment in this respect was 1 -MCP with 30 gL-I sucrose. 1-MCP treatment significantly increased the chlorophyll content, as compared to the control or the "Spring" treatment. The highest values in this respect were obtained by 1 -MCP treatment alone or with the lowest level of sucrose. The effect of these treatments on the pH of solutions is discussed.

The synergistic effect of maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione) and sucrose on vase life of cut roses

2002 ◽  
Vol 42 (5) ◽  
pp. 637
Author(s):  
K.-L. Huang ◽  
L.-J. Liao ◽  
R.-S. Shen ◽  
W.-S. Chen ◽  
Y.-H. Lin
Keyword(s):  
Synergistic Effect ◽  
Water Uptake ◽  
Water Loss ◽  
Maleic Acid ◽  
Ethylene Production ◽  
Acid Hydrazide ◽  
Vase Life ◽  
Cut Flowers ◽  
Postharvest Treatment ◽  
Cut Rose

Continuous postharvest treatment of cut rose flowers (Rosa hybrida L. cv. Diana) with maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione, MH) at 560.5 8-hydroxyquinoline sulfate (HQS) at 388.4 HQS, MH + HQS or sucrose + HQS treatments. The longevity of flowers in MH + sucrose in combination with HQS was extended for 18 days after vase treatments, whereas the longevity of cut flowers was only 4, 6 and 8 days for HQS, MH + HQS and sucrose + HQS, respectively. Cut roses treated with MH + sucrose + HQS in vase solution exhibited greater water uptake and less water loss than those in HQS. The concentrations of various sugars in petals were highest in the sucrose + HQS treatment, and MH + sucrose + HQS > MH + HQS > HQS. Ethylene production was significantly lower in sucrose + HQS or MH + sucrose + HQS treatments in comparison to MH + HQS, or HQS.

scholarly journals Effect of Dry and Wet Storage at Different Temperatures on the Vase Life of Cut Flowers

2001 ◽  
Vol 11 (2) ◽  
pp. 199-202 ◽  
Author(s):  
Juan-Carlos Cevallos ◽  
Michael S. Reid
Keyword(s):  
Storage Temperature ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Cut Flowers ◽  
Tulipa Gesneriana ◽  
Wet Storage ◽  
Different Temperatures ◽  
Narcissus Pseudonarcissus ◽  
C 32 ◽  
Storage Temperatures

After storage at different temperatures for a simulated transportation period, the vase lives at 20 °C (68 °F) of carnations (Dianthus caryophyllus `Imperial White'), daffodils (Narcissus pseudonarcissus `King Alfred'), iris (Iris hollandica `Telstar'), killian daisies (Chrysanthemum maximum), paperwhite narcissus (Narcissus tazetta `Paperwhite'), roses (Rosa {XtimesX}hybrida `Ambiance'), and tulips (Tulipa gesneriana) decreased with increasing storage temperature. There were no significant differences between the vase life of flowers stored dry and flowers stored in water when storage temperatures were from 0 to 10 °C (32 to 50 °F). The vase life after wet storage at temperatures of 12.5 °C (54.5 °F) and greater was significantly higher than vase life after dry storage at those temperatures for all the flowers studied. Iris and carnation flowers survived storage at 15 and 20 °C (59 and 68 °F) only when stored in water.

scholarly journals THE EFFECT OF POLYAMINES ON THE SENESCENCE OF CARNATION PETALS.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 617d-617
Author(s):  
Ki-Cheol Son ◽  
Y. Chae
Keyword(s):  
Ethylene Production ◽  
Dianthus Caryophyllus ◽  
Acc Synthase ◽  
The Other ◽  
Fresh Weight ◽  
Exact Role ◽  
High Concentrations ◽  
Acc Content ◽  
Carnation Petals

The interaction between polyamines and ethylene is still not clear for floral tissues. The aim of the present paper is to examine the senescence on the isolated petals of carnation (Dianthus caryophyllus cv. Desio) but not the whole flower in an attempt to clarify the exact role of polyamines. Petals were treated with putrescine (Put; 0.0, 1.0, 10mM), spermidine (Spd; 0.0, 1.0, 10mM), spermine (Spn; 0.0, 1.0, 10mM), Put+Spd (1.0mM), Put+Spn (1.0mM). The fresh weight of petals in all 10mM treatment was decreased less than those in the other treatments at all times but there were no significant differences. The differences in ethylene production were significant. In petals maintained in 10mM of polyamines, ethylene production was completely inhibited until 13 days and senescence was considerably retarded. However, ethylene productions in 1.0mM polyamines treatments were delayed 2-3 days with reduced amounts. These results suggest that high concentrations of polyamines retard senescence and completely inhibit ethylene production. ACC content, activities of ACC synthase and SAM decarboxylase were analyzed. Finally, the role of SAM in ethylene and polyamines biosynthesis will be discussed.

scholarly journals Differential Response of Carnation Varieties (Dianthus caryophyllus L.) to Different Levels of Boron under Polytunnels

2020 ◽  
Vol 9 (2) ◽  
pp. 133-145
Author(s):  
Januka Dahal ◽  
Debraj Adhikari ◽  
Umed Kumar Pun
Keyword(s):  
Block Design ◽  
Dianthus Caryophyllus ◽  
Growth Yield ◽  
Ultra Violet ◽  
Leaf Length ◽  
Optimum Dose ◽  
Vase Life ◽  
Cut Flowers ◽  
Internodal Length ◽  
Flower Diameter

Carnation (Dianthus caryophyllus L.) is one of the most commercially important cut flowers of the world. It can be used for cut flowers, bedding, edging, boarders, pots and rock garden. Four Spanish carnation varieties were tested with three levels of boron under ultra-violet stabilized polytunnels from August 2017 to April 2018 at Godawari, Nepal. The objective of the research was to evaluate vegetative, floral, and vase life parameters in relations to growth, yield and quality of carnation. The research was conducted in a randomized complete block design with 3 replications. The variety Baltico had the maximum leaf length (19.3 cm), numbers of lateral branches (10.5), internodal length (5.1 cm), flower diameter (8.6 cm), and vase life (24.0 days). The numbers of nodes (5) and flower weight (25.4 g) were highest in the variety Vinco with the lowest cracked area on the stem (0.2 cm2/ crack). The numbers of stem cracks per plant significantly (p ≤0.01) reduced with an increasing level of boron though non-significant relation was observed on 60 ppm and 120 ppm. Variety Baltico was found as a better variety compared to the Vinco, Cervantes and Master varieties according to the vegetative, floral, yield and vase life parameters. Among the levels of boron tested, 60 ppm was found as the optimum dose. Thus, variety Baltico and 60 ppm boron is suggested to be used at the climatic condition of Lalitpur.

scholarly journals Physiological and Molecular Characterization of the Response to Ethylene during Senescence of Carnation Genotypic Variants

1995 ◽  
Author(s):  
William Woodson ◽  
Shimon Mayak ◽  
Haim Rabinowitch
Keyword(s):  
Ethylene Production ◽  
Dianthus Caryophyllus ◽  
Acc Oxidase ◽  
Acc Synthase ◽  
Early Response ◽  
Vase Life ◽  
Petal Senescence ◽  
Ethylene Treatment ◽  
Novel Proteins ◽  
Exogenous Ethylene

The senescence of carnation (Dianthus caryophyllus L.) flowers is associated with increased production of the phytohormone ethylene, which in turn serves to initiate and regulate the processes involved in programmed petal death. We investigated the regulation of ethylene production and petal senescence in carnation. Several carnation genotypes were identified that exhibited extended vase-life in comparison to flowers from typical commercial cultivars. The capacity of these genotypes to produce ethylene during postharvest vase-life and to respond to exogenous ethylene was investigated. Several genotypes, represented by 'Sandrosa' and 87-37G produced little ethylene durig their postharvest vase-life and as a result failed to exhibit the symptoms (in-rolling and wilting) typical of flowers producing elevated levels of ethylene. These genotypes were further separated by their capacity to respond to exogenous ethylene by both increased ethylene synthesis and premature petal senescence. In one case a genotype (799) was identified that was not capable of responding to exogenous ethylene by either increased ethylene production or premature petal senescence. The regulation of ethylene production during petal senescence was investigated both at the enzyme and gene levels. A full length cDNA was identified for the petal senescence-related ACC synthase gene. Utilizing this, and other ethylene biosynthetic pathway cDNA probes, an increase in both ACC synthase and ACC oxidase mRNAs were detected following ethylene treatment. An increase in ACC oxidase mRNA and enzyme activity was detected within 2-3 h following ethylene treatment, indicating the expression of this gene is an early response to ethylene. An investigation into the expression of novel proteins during petal senescence revealed a number of polypeptides increased in abundance and possibly play a role in the regulation or biochemical processes of senescence. One polypeptide of 70 kDa was identified as being encoded by the previously characterized gene SR12 and possibly represents a b-galactosidase involved in the remobilization of carbohydrates during senescence.

scholarly journals Psidium guajavaandPiper betleLeaf Extracts Prolong Vase Life of Cut Carnation (Dianthus caryophyllus) Flowers

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
M. M. Rahman ◽  
S. H. Ahmad ◽  
K. S. Lgu
Keyword(s):  
Tap Water ◽  
Dianthus Caryophyllus ◽  
Psidium Guajava ◽  
Vase Life ◽  
Piper Betle ◽  
Cut Flowers ◽  
Leaf Extracts ◽  
Antibacterial And Antifungal Activities ◽  
Flower Diameter ◽  
Copper Coin

The effect of leaf extracts ofPsidium guajavaandPiper betleon prolonging vase life of cut carnation flowers was studied. “Carola” and “Pallas Orange” carnation flowers, at bud stage, were pulsed 24 hours with a floral preservative. Then, flowers were placed in a vase solution containing sprite and a “germicide” (leaf extracts ofP. guajavaandP. betle, 8-HQC, or a copper coin). Flowers treated with 8-HQC, copper coin, and leaf extracts had longer vase life, larger flower diameter, and higher rate of water uptake compared to control (tap water). The leaf extracts ofP. guajavaandP. betleshowed highest antibacterial and antifungal activities compared to the other treatments. Both showed similar effects on flower quality as the synthetic germicide, 8-HQC. Therefore, these extracts are likely natural germicides to prolong vase life of cut flowers.

scholarly journals Flowering profile and ethylene production of a new carnation subspecies ‘Mini-tiara’ 

2018 ◽  
Vol 45 (No. 4) ◽  
pp. 187-192
Author(s):  
Shigeru Satoh ◽  
Yoshihiro Nomura ◽  
Sayuri Takeuchi ◽  
Ryusuke Seo
Keyword(s):  
Ethylene Production ◽  
Interspecific Cross ◽  
Dianthus Caryophyllus ◽  
Middle Part ◽  
Wild Type ◽  
Cut Flowers ◽  
Display Time ◽  
Ethylene Evolution ◽  
New Subspecies

Ethylene is involved in the senescence of carnation flowers. It is synthesized abundantly after full opening of the flowers and accelerates wilting of petals, which results in withering of the flowers. We investigated the possible involvement of ethylene production in the loss of display value of cut flowers of ‘Mini-tiara’ carnation, a new subspecies derived from Dianthus caryophyllus L. by interspecific cross with wild-type Dianthus species (wild pinks). Flowers of ‘Mini-tiara’ carnation have a unique morphology with pointed-shaped petals, some of which in the middle part of the flowers stand straight and build a dome throughout the display time, and lose their display value when the dome collapses by bending all petals outside (full opening of flowers). Ethylene evolution from ‘Mini-tiara’ carnation was not detected from flowers with upstanding petals, but occurred several days after the collapse of the dome (after full opening of the flowers), the time they already lost their display value. These findings indicated that ethylene production is not engaged in the loss of display value of ‘Mini-tiara’ carnation. 

scholarly journals Using Arachis pintoi leaf extracts in biosynthesis of silver nanoparticles for improving the vase life of cut carnation (Dianthus caryophyllus L.)

2020 ◽  
Vol 17 (4) ◽  
pp. 673-688
Author(s):  
Tran Lap Xuan ◽  
Le Ba Le ◽  
Le Thi Anh Tu
Keyword(s):  
Silver Nanoparticles ◽  
Dianthus Caryophyllus ◽  
Postharvest Quality ◽  
Vase Life ◽  
Cut Flowers ◽  
Leaf Extracts ◽  
Solution Ph ◽  
Arachis Pintoi ◽  
Flower Diameter ◽  
Average Size

A biological method for synthesizing silver nanoparticles (SNPs) using the leaf extracts of Arachis pintoi Krapov. & W.C. Greg. was developed.  The optimum conditions of input materials were found with leaf autoclaving in 15 min, 20 g fresh leave, and 4 mM of silver nitrate (AgNO3). To study the role of time, temperature, and solution pH of the reaction, varying time reaction (5, 30, 60, 90, 120, 150, and 180 min), temperature reaction (10, 20, 30, 40, and 50oC) and pH of the solution (1, 3, 5, 7, 9, and 11) were investigated. The optimal biosynthesis conditions were achieved in 180 min of reaction time at 50oC and pH 11. The obtained nanoparticles have spherical and oblong in shape with average size of 26.4 nm. The SNPs in 4 concentrationss (5, 15, 25, and 35 ppm) combined with and without 2% sucrose extended vase life, enlarged flower diameter, and maintained increase the relative fresh weight with vase solution uptake rate. SNPs inhibited significantly the bacterial growth in the stem end and vase solution, reduced the blockage in stems and therefore promoted the postharvest quality of carnation cut flowers. Out of the treatments, administration of 5 ppm SNPs with 2% sucrose of vase solution gave the best results for all parameters. The biosynthesis SNPs could be applied as a promising preservative solution for carnation cut flowers.

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