scholarly journals Flowering profile and ethylene production of a new carnation subspecies ‘Mini-tiara’ 

2018 ◽  
Vol 45 (No. 4) ◽  
pp. 187-192
Author(s):  
Shigeru Satoh ◽  
Yoshihiro Nomura ◽  
Sayuri Takeuchi ◽  
Ryusuke Seo
Keyword(s):  
Ethylene Production ◽  
Interspecific Cross ◽  
Dianthus Caryophyllus ◽  
Middle Part ◽  
Wild Type ◽  
Cut Flowers ◽  
Display Time ◽  
Ethylene Evolution ◽  
New Subspecies

Ethylene is involved in the senescence of carnation flowers. It is synthesized abundantly after full opening of the flowers and accelerates wilting of petals, which results in withering of the flowers. We investigated the possible involvement of ethylene production in the loss of display value of cut flowers of ‘Mini-tiara’ carnation, a new subspecies derived from Dianthus caryophyllus L. by interspecific cross with wild-type Dianthus species (wild pinks). Flowers of ‘Mini-tiara’ carnation have a unique morphology with pointed-shaped petals, some of which in the middle part of the flowers stand straight and build a dome throughout the display time, and lose their display value when the dome collapses by bending all petals outside (full opening of flowers). Ethylene evolution from ‘Mini-tiara’ carnation was not detected from flowers with upstanding petals, but occurred several days after the collapse of the dome (after full opening of the flowers), the time they already lost their display value. These findings indicated that ethylene production is not engaged in the loss of display value of ‘Mini-tiara’ carnation. 

scholarly journals Effects of Anti-Ethylene Treatments on Ethylene Production and Antioxidant Activities in Cut Spray Carnation

2012 ◽  
Vol 20 (2) ◽  
pp. 173-182
Author(s):  
Mahnaz Karimi ◽  
Moazzam Hassanpour Asil ◽  
Ghorbanali Nematzadeh ◽  
Hedayat Zakizadeh
Keyword(s):  
Antioxidant Enzymes ◽  
Ethylene Production ◽  
Enzyme Activities ◽  
Antioxidant Activities ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Cut Flowers ◽  
Acc Content ◽  
Oxyacetic Acid

Abstract The present investigation was aimed to study changes in ethylene production, ACC content and antioxidant enzymes of cut spray carnation (Dianthus caryophyllus L.) flowers that had been treated with amino-oxyacetic acid (AOA), benzyladenine (BA) and 1-methylcyclopropene (1-MCP). Maximum vase life in ‘Optima’ spray carnations was obtained with 0.6 mg l-1 1-MCP. Ethylene production was significantly decreased by AOA at concentrations over 100 mg l-1, BA at 30 mg l-1, and 1-MCP at all concentrations, compared with the control. A significant increase in ACC content was observed in 1-MCP treated cut flowers compared with the control. However, the decline in ACC content was observed after using 100 or 150 mg l-1 AOA. A significant increase in SOD, CAT and POX enzyme activities was observed in the treatment with 0.6 mg l-1 1-MCP.

scholarly journals 24-Epibrasinolide Modulates the Vase Life of Lisianthus Cut Flowers by Modulating ACC Oxidase Enzyme Activity and Physiological Responses

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 995
Author(s):  
Mohammad Darvish ◽  
Habib Shirzad ◽  
Mohammadreza Asghari ◽  
Parviz Noruzi ◽  
Abolfazl Alirezalu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Water Absorption ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Postharvest Quality ◽  
Vase Life ◽  
Dependent Manner ◽  
Cut Flowers ◽  
Oxidase Enzyme

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.

scholarly journals Direct Interaction between the Subunit RAP30 of Transcription Factor IIF (TFIIF) and RNA Polymerase Subunit 5, Which Contributes to the Association between TFIIF and RNA Polymerase II

2001 ◽  
Vol 276 (15) ◽  
pp. 12266-12273 ◽  
Author(s):  
Wenxiang Wei ◽  
Dorjbal Dorjsuren ◽  
Yong Lin ◽  
Weiping Qin ◽  
Takahiro Nomura ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Middle Part ◽  
Wild Type ◽  
Binding Region ◽  
Pol Ii ◽  
Transcription Factor Iif ◽  
B Virus

The general transcription factor IIF (TFIIF) assembled in the initiation complex, and RAP30 of TFIIF, have been shown to associate with RNA polymerase II (pol II), although it remains unclear which pol II subunit is responsible for the interaction. We examined whether TFIIF interacts with RNA polymerase II subunit 5 (RPB5), the exposed domain of which binds transcriptional regulatory factors such as hepatitis B virus X protein and a novel regulatory protein, RPB5-mediating protein. The results demonstrated that RPB5 directly binds RAP30in vitrousing purified recombinant proteins andin vivoin COS1 cells transiently expressing recombinant RAP30 and RPB5. The RAP30-binding region was mapped to the central region (amino acids (aa) 47–120) of RPB5, which partly overlaps the hepatitis B virus X protein-binding region. Although the middle part (aa 101–170) and the N-terminus (aa 1–100) of RAP30 independently bound RPB5, the latter was not involved in the RPB5 binding when RAP30 was present in TFIIF complex. Scanning of the middle part of RAP30 by clustered alanine substitutions and then point alanine substitutions pinpointed two residues critical for the RPB5 binding inin vitroandin vivoassays. Wild type but not mutants Y124A and Q131A of RAP30 coexpressed with FLAG-RAP74 efficiently recovered endogenous RPB5 to the FLAG-RAP74-bound anti-FLAG M2 resin. The recovered endogenous RPB5 is assembled in pol II as demonstrated immunologically. Interestingly, coexpression of the central region of RPB5 and wild type RAP30 inhibited recovery of endogenous pol II to the FLAG-RAP74-bound M2 resin, strongly suggesting that the RAP30-binding region of RPB5 inhibited the association of TFIIF and pol II. The exposed domain of RPB5 interacts with RAP30 of TFIIF and is important for the association between pol II and TFIIF.

Evolution of ethylene by Saccharomyces cerevisiae as influenced by the carbon source for growth and the presence of air

1978 ◽  
Vol 24 (6) ◽  
pp. 637-642 ◽  
Author(s):  
K. C. Thomas ◽  
Mary Spencer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Carbon Source ◽  
Ethylene Production ◽  
Atp Synthesis ◽  
Yeast Cells ◽  
Yeast Culture ◽  
Wild Type ◽  
Yeast Saccharomyces Cerevisiae ◽  
Absolute Requirement ◽  
Wild Type Yeast

Effects of the carbon source and oxygen on ethylene production by the yeast Saccharomyces cerevisiae have been studied. The amounts of ethylene evolved by the yeast culture were less than those detected in the blank (an equal volume of uninoculated medium), suggesting a net absorption of ethylene by the yeast cells. Addition of glucose to the lactate-grown yeast culture induced ethylene production. This glucose-induced stimulation of ethylene production was inhibited to a great extent by cycloheximide. Results suggested that the yeast cells in the presence of glucose synthesized an ethylene precursor and passed it into the medium. The conversion of this precursor to ethylene might be stimulated by oxygen. The fact that ethylene was produced by the yeast growing anaerobically and also by respiration-deficient mutants isolated from the wild-type yeast suggested that mitochondrial ATP synthesis was not an absolute requirement for ethylene biogenesis.

scholarly journals Daminozide, Root Pruning, Trunk Scoring, and Trunk Ringing Effects on Fruit Ripening and Storage Behavior of `McIntosh' Apple

1991 ◽  
Vol 116 (2) ◽  
pp. 195-200 ◽  
Author(s):  
D.C. Elfving ◽  
E.C. Lougheed ◽  
R.A. Cline
Keyword(s):  
Ethylene Production ◽  
Fruit Size ◽  
Soluble Solids ◽  
Starch Hydrolysis ◽  
Root Pruning ◽  
Full Bloom ◽  
Ethylene Evolution ◽  
Flesh Firmness ◽  
Solids Concentration ◽  
Storage Behavior

A midsummer foliar daminozide (DZ) application (750 mg a.i./liter) to `Macspur McIntosh'/M.7 apple trees (Malus domestics Borkh.) reduced preharvest drop and retarded flesh firmness loss and starch hydrolysis when tested at harvest; DZ also reduced fruit ethylene production at harvest and after 19 weeks of storage at 0.5C. Root pruning at full bloom (May) resulted in increased soluble solids concentration (SSC) and firmer flesh and less starch hydrolysis at harvest, but not consistently each year. Full-bloom root pruning reduced the incidence of stem-cavity browning and brown core, but again not each year. Full-bloom root pruning did not influence ethylene evolution at harvest but did reduce post-storage ethylene evolution in two of three seasons. Full-bloom root pruning generally was less effective than DZ in altering fruit behavior, while root pruning later than full bloom had virtually no effect. Trunk scoring or ringing increased SSC and retarded loss of flesh firmness before harvest and following storage, but had little effect on starch hydrolysis. Scoring or ringing decreased incidence of some disorders and reduced post-storage ethylene evolution, although these treatments had little effect on ethylene production at harvest. Trunk scoring influenced some fruit characteristics more strongly than DZ. Fruit size was not affected by any treatment in any year. Chemical name used: butanedioic acid mono (2,2 -dimethylhydrazide) (daminozide).

scholarly journals Prolonging the vase life of cut Carnation 'GIOKO' by using different chemicals

2008 ◽  
Vol 14 (4) ◽  
Author(s):  
O. Terék ◽  
F. A. S. Hassan ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Untreated Control ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Distilled Water ◽  
Cut Flowers ◽  
Chemical Treatments ◽  
All Solutions ◽  
Separate Treatment ◽  
Flower Diameter ◽  
Positive Effect

Cut flowers of Dianthus caryophyllus L. cv. GIOKO were treated with different concentrations of sucrose and in combination with 1­methylcyclopropene (1-MCP) to compare the effect of these treatments with floral preservative (`Spring') on the longevity of flowers. Distilled water was used for preparing all solutions. The control flowers were held in distilled water. Clorox at 2 mL- I was added to all treatments containing sucrose and it was also applied as a separate treatment. The vase life of cut carnations was significantly prolonged due to the use of chemical treatments, as compared to the untreated control. The longest vase life (18.33 days) was obtained by using 1-MCP 0.5 g m-3 for 6 h treatment. All concentrations of sucrose had a positive effect on flower diameter. The best treatment in this respect was 1 -MCP with 30 gL-I sucrose. 1-MCP treatment significantly increased the chlorophyll content, as compared to the control or the "Spring" treatment. The highest values in this respect were obtained by 1 -MCP treatment alone or with the lowest level of sucrose. The effect of these treatments on the pH of solutions is discussed.

Leaf area development of ABA-deficient and wild-type peas at two levels of nitrogen supply

2003 ◽  
Vol 30 (7) ◽  
pp. 777 ◽  
Author(s):  
Ian C. Dodd
Keyword(s):  
Growth Response ◽  
Leaf Growth ◽  
Nitrogen Supply ◽  
Genotypic Differences ◽  
Wild Type ◽  
Ethylene Evolution ◽  
Relative Water ◽  
Ca 50 ◽  
Area Development ◽  
Water Contents

The ABA-deficient wilty pea (Pisum sativum L.) and its wild-type (WT) were grown at two levels of nitrogen supply (0.5 and 5.0 mM) for 5–6 weeks from sowing, to determine whether leaf ABA status altered the leaf growth response to N deprivation. Plants were grown at high relative humidity to prevent wilting of the wilty peas. Irrespective of N supply, expanding wilty leaflets had ca 50% less ABA than WT leaflets but similar ethylene evolution rates. Fully expanded wilty leaflets had lower relative water contents (RWC) and were 10–60% smaller in area (according to the node of measurement) than WT leaflets. However, there were no genotypic differences in plant relative leaf expansion rate (RLER). Growth of both genotypes at 0.5 mM N increased the RWC of fully expanded leaflets, but did not alter ethylene evolution or ABA concentration of expanding leaflets. Plants grown at 0.5 mM N showed a 20–30% reduction in RLER, which was similar in magnitude in both wilty and WT peas. Thus,�leaf ABA status did not alter the leaf growth response to N deprivation.

Do low-ethylene-producing transgenic canola (Brassica napus) plants expressing the ACC deaminase gene differ from wild-type plants in response to UVB radiation?

2007 ◽  
Vol 85 (2) ◽  
pp. 148-159 ◽  
Author(s):  
Mirwais M. Qaderi ◽  
M. Anisul Islam ◽  
David M. Reid ◽  
Saleh Shah
Keyword(s):  
Brassica Napus ◽  
Transgenic Plants ◽  
Photosynthetic Pigment ◽  
Physiological Parameters ◽  
Ultraviolet B ◽  
Differential Sensitivity ◽  
Plant Responses ◽  
Uvb Radiation ◽  
Wild Type ◽  
Ethylene Evolution

Few studies have considered ethylene involvement in plant responses to ultraviolet-B (UVB) radiation. We studied the responses to UVB radiation of one wild-type (WT, ‘Westar’) canola (Brassica napus L.) with normal ethylene evolution and two transgenic (C1, C2) lines with lower ethylene evolution. Canola plants were grown under biologically effective levels of UVB (UVBBE) radiation: 0.03 (low), 4.88 (medium), and 9.78 (high) kJ·m–2·d–1 in controlled-environment growth chambers. The growth and physiological parameters of the plants were measured. Of the two transgenic lines, C1 demonstrated higher ethylene evolution than C2 but lower than WT. The lowest aboveground and belowground biomass was found with exposure to high UVB radiation. WT produced more biomass than C2. Net CO2 assimilation and transpiration did not vary among plant lines or UVB treatments. Water-use efficiency was lower under high UVB radiation than under low UVB. The quantum yield of photosystem II was higher for C2 than for either WT or C1. WT did not differ from transgenic plants in respect to photosynthetic pigments and UV-screening compounds. Photosynthetic pigment concentration decreased, but concentration of UV-screening compounds, thickness of epicuticular wax, and the rate of root hydraulic conductance were increased by exposure to UVB radiation. While there appears to be a lack of ethylene involvement in some of the measured physiological parameters, the transgenic plants exhibited differential sensitivity to UVB in a few key measured parameters.

The synergistic effect of maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione) and sucrose on vase life of cut roses

2002 ◽  
Vol 42 (5) ◽  
pp. 637
Author(s):  
K.-L. Huang ◽  
L.-J. Liao ◽  
R.-S. Shen ◽  
W.-S. Chen ◽  
Y.-H. Lin
Keyword(s):  
Synergistic Effect ◽  
Water Uptake ◽  
Water Loss ◽  
Maleic Acid ◽  
Ethylene Production ◽  
Acid Hydrazide ◽  
Vase Life ◽  
Cut Flowers ◽  
Postharvest Treatment ◽  
Cut Rose

Continuous postharvest treatment of cut rose flowers (Rosa hybrida L. cv. Diana) with maleic acid hydrazide (1.2-dihydro-3,6-pyridazinedione, MH) at 560.5 8-hydroxyquinoline sulfate (HQS) at 388.4 HQS, MH + HQS or sucrose + HQS treatments. The longevity of flowers in MH + sucrose in combination with HQS was extended for 18 days after vase treatments, whereas the longevity of cut flowers was only 4, 6 and 8 days for HQS, MH + HQS and sucrose + HQS, respectively. Cut roses treated with MH + sucrose + HQS in vase solution exhibited greater water uptake and less water loss than those in HQS. The concentrations of various sugars in petals were highest in the sucrose + HQS treatment, and MH + sucrose + HQS > MH + HQS > HQS. Ethylene production was significantly lower in sucrose + HQS or MH + sucrose + HQS treatments in comparison to MH + HQS, or HQS.

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