scholarly journals ANTIOXIDANT ACTIVITY TEST OF ANDROGRAPHOLIDE IN BITTER HERBS USING DPPH SCAVENGING

2017 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Ni Made Pitri Susanti ◽  
Ni Kadek Warditian ◽  
I Made Agus Gelgel Wirasuta
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Pharmacological Activities ◽  
Inhibition Effect ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Scavenging ◽  
Anti Inflammation

Abstract Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic, anticancer, antihyperlipidemic and anti-inflammation. Andrographolide is a diterpene compounds contained in bitter herbs. It is known that andrographolide compound responsible for the pharmacological activity of the bitter herbs. This study investigated DPPH free radical scavenging activity from andrographolide diterpene lactone. This study was initiated with the isolation of andrographolide compound from bitter herbs and then testing their DPPH free radical scavenging. The results suggested that andrographolide had IC50 value of 5.45 mg. This means andrographolide has 50% DPPH inhibition effect, i.e 5.45 mg.

scholarly journals Pharmacognostic review of Astercantha longifolia (L.) Nees, & its possible use in prevention of COVID-19

2021 ◽  
Vol 6 (4) ◽  
pp. 231-236
Author(s):  
Shiva Prasad Mohanty ◽  
◽  
Kshirabdhi Tanaya Rautaray ◽  
Keyword(s):  
Free Radicals ◽  
Viral Infections ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Viral Disease ◽  
Free Radical Scavenging ◽  
Point Of View ◽  
Scavenging Activity ◽  
Pharmacological Activities ◽  
Annual Herb

Astercantha longifolia is an annual herb from Acanthaceae family found almost as weed throughout plainlands, all over India. It is very much important from Ayurveda point of view for its curative role in many disease conditions. It is called as Kokilakshya in Ayurveda & Talamakhanna in Unani system. Its importance in Ayurveda can be judged by its visibility in Samhitas & Nighantus. India is a young country with billion plus population & having second highest in COVID affected numbers. Being a viral disease, immunity boosting drugs are the need of the hour & Astercantha longifolia (L) Nees or Kokilakshya fits in this situation perfectly. It has many pharmacological activities and the best action is, its free radical scavenging activity. Viral infections unleash storms of cytokinin, which produces abundant free radicals & the complications of COVID 19 are mainly due to these free radicals [1] . So, keeping all these qualities in mind, an honest attempt is made to enumerate those details and justify its perspective utility in Covid 19.

IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ZIZIPHUS RUGOSA LAM. LEAVES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 69-75
Author(s):  
S Parashar ◽  
V. Uplanchiwar ◽  
R. K. Gautam ◽  
S. Goyal ◽  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value

Ziziphus rugosa Lam. belongs to the family Rhamnaceae and is found chiefly in deciduous and semi evergreen forest of Western Ghats. The present research was undertaken to establish in vitro antioxidant and in vivo hepatoprotective activity of ethanolic extract of Z.rugosa Lam. leaves. The powdered leaves of Z. rugosa were extracted with ethanol and preliminary phytochemical screening was performed for the presence of various phytoconstituents. DPPH assay and β-glucuronidase inhibition assay were selected for the free radical scavenging activity. For the assessment of hepatoprotective activity, alcohol and CCl4 induced hepatotoxicity model were used. The phytochemical analysis of ethanolic extract showed the presence of alkaloids, saponins and flavonoids. The extract exhibited concentration dependent radical scavenging activity with an IC50 value of 61.88 μg/ml and β –glucoronidase inhibition activity with an IC50 value of 70.61 μg/ml. It was speculated that the Z. rugosa Lam. ethanolic extract shows dosedependent hepatoprotective activity which is equivalent with the standard drug Silymarin. The inhibition of free radicals or free radical scavenging activity is significant in the protection against CCl4 and alcohol induced hepatopathy. Hence, it is likely that the antioxidant activity of ethanolic extract of Z. rugosa Lam. might contribute to the hepatoprotective action.

scholarly journals ANTICANCER AND FREE RADICAL SCAVENGING POTENTIAL OF THE MARINE ALGICOLOUS ENDOPHYTIC FUNGUS CLADOSPORIUM UREDINICOLA

2019 ◽  
pp. 520-524
Author(s):  
VASAVI THIRUMALANADHUNI ◽  
LAVANYA LATHA YERRAGURAVAGARI ◽  
VANI MATHAKALA ◽  
UMA MAHESWARI DEVI PALEMPALLI
Keyword(s):  
Free Radical ◽  
Endophytic Fungus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Normal Cells ◽  
Ic50 Value ◽  
Marine Brown Alga

Objective: The objective was to study the antioxidant and anticancer potential of the endophytic fungus Cladosporium uredinicola, isolated from the marine brown alga Dictyota dichotoma. Methods: Anticancer effect of the endophytic fungal extract was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay using MDA-MB-231 human mammary adenocarcinoma cells as in vitro cancer models. 3T3-L1 pancreatic adipocytes were used as in vitro models for the evaluation of cytotoxic activity against normal cells using MTT assay. Free radical scavenging activity was assessed by 2, 2-diphenyl-1- picrylhydrazyl (DPPH) assay. Results: The ethyl acetate extract of the endophytic fungus showed potent cytotoxic activity against MDA-MB-231 human breast adenocarcinoma cell lines with an inhibitory concentration (IC50) value of 373 μg/ml and a very mild cytotoxic effect on 3T3-L1 Cells with an IC50 value of 2403 μg/ml. DPPH free radical scavenging assay of the extract showed an IC50 value of 359 μg/ml indicating its potential free radical scavenging activity. Conclusion: The results indicated that the endophytic fungus C. uredinicola, isolated from the marine brown alga D. dichotoma, acts as a potential source for anticancer and antioxidant metabolites. Moreover, these anticancer metabolites were observed to be less toxic to the normal cells, which make them prospective therapeutic agents.

scholarly journals ANALISIS FRAKSI AKTIF EKSTRAK DAUN GEDI HIJAU (Abelmoschus manihot L.) DALAM MENANGKAL RADIKAL BEBAS DPPH

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 187
Author(s):  
Maria T Atuani ◽  
Sri Sudewi ◽  
Defny S Wewengkang
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Mobile Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Fraction I

ABSTRACT Gedi (Abelmoschus manihot L.) is a tropical plant of family malvaceae, traditionally known in North Sulawesi as a vegetable plant. Gren gedi leaves from Bitung are known to have activity as free radical scavenging at extract level but have not been tested at the fraction level. This study aims to determine the free radical scavenging activity of the green gedi leaf fractions in counteraction DPPH (1,1-diphenil-2-picrylhydarzyl) free radical. Extraction was done by maceration method using ethanol solvent. The preliminary test of free radical scavenging was carried out by thin layer chromatography (TLC) using the stationary phase of silica gel F254 with the mobile phase of n-hexane : chloroform (1:2) and sprayed with DPPH 0,08 mM. Then it was fractionated using vacuum liquid chromatography (VLC) with the mobile phase based on its polarity, such as combination of n-hexane and ethyl acetate. Each fraction obtained was tested for free radical scavenging activity showed whitish yellow patches on a purple background, which indicates that extracts have free radical scavenging activity. The spectrophotometer measurement results showed that each leaf of green gedi leaves had weak free radical scavenging activity, but still was a free radical scavenger, ie fraction I had an IC50 value of 169,69 μg/mL; fraction II was 826,52 μg/mL; fraction III was 311,13 μg/mL; and fraction IV was 246,80 μg/mL. Fraction V has an IC50 value of 2294,64 μg/mL, this is indicates that the fraction V has no activity as a free radical scavenging compound, because it has very large IC50 value. While vitamin C as a comparison has very strong free radical scavenging activity with IC50 of 3,22 μg/mL. Keywords: Fraction, Extract, Abelmoschus manihot L., DPPH Method. ABSTRAK Gedi (Abelmoschus manihot L.) merupakan tumbuhan tropis family malvaceae, secara tradisional telah lama dikenal di Sulawesi Utara sebagai tanaman sayuran. Daun gedi hijau yang berasal dari Bitung diketahui memiliki aktivitas sebagai penangkap radikal bebas pada tingkat ekstrak namun belum diuji pada tingkat fraksi. Penelitian ini bertujuan untuk mengetahui aktivitas penangkap radikal bebas dari fraksi-fraksi daun gedi hijau dalam menangkal radikal bebas DPPH (1,1-diphenil-2-picrylhydarzyl). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol. Uji pendahuluan aktivitas penangkap radikal bebas dilakukan secara kromatografi lapis tipis (KLT) menggunakan fase diam silika gel F254 dengan fase gerak n-heksana : kloroform (1:2) dan disemprot DPPH 0,08mM. Kemudian difraksinasi menggunakan kromatografi cair vakum (KCV) dengan fase gerak berdasarkan kepolarannya, yakni kombinasi n-heksana dan etil asetat. Hasil uji pendahuluan aktivitas penangkap radikal bebas secara KLT menunjukkan adanya bercak kuning keputih-putihan dengan latar belakang ungu, yang menandakan ekstrak memiliki aktivitas penangkap radikal bebas. Hasil pengukuran secara spektrofotometer menunjukkan bahwa setiap fraksi daun gedi hijau memiliki aktivitas penangkap radikal bebas yang lemah, namun tetap bersifat sebagai penangkap radikal bebas, yakni fraksi I memiliki nilai IC50 sebesar 169,69 μg/mL; fraksi II 826,52 μg/mL; fraksi III 311,13 μg/mL; dan fraksi IV 246,80 μg/mL. Fraksi V memiliki nilai IC50 sebesar 2294,64 μg/mL, menandakan fraksi V tidak memiliki aktivitas sebagai senyawa penangkap radikal bebas, karena memiliki nilai IC50 yang sangat besar. Sedangkan vitamin C sebagai pembanding memiliki aktivitas penangkap radikal bebas sangat kuat dengan IC50 sebesar 3,22 μg/mL.Kata Kunci : Fraksi, Ekstrak, Abelmoschus manihot L., Metode DPPH.

scholarly journals Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay

2019 ◽  
pp. 1-6
Author(s):  
Alhassan M. Garba ◽  
Habiba R. Isa ◽  
Sadiq Abubakar ◽  
Saudat Ja’afar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Dried and powdered leaves of Albizia chevalieri were extracted using ethanol. The extract was fractionated to give methanol, chloroform and pet-ether. The four extracts obtained; ethanol, chloroform, methanol and pet-ether were evaluated for antioxidant activity using 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) free radical scavenging activity assay. The results of the DPPH scavenging activity indicated a concentration-dependent antioxidant activity. The DPPH scavenging activity of the ethanol, chloroform and methanol extracts were found to be promising. There is no significant difference in the antioxidant activity between the ethanol, chloroform and methanol extracts with that of standard Ascorbic acid at 10, 25, 250 and 500 μg/ml concentrations. This showed that the ethanol, chloroform and methanol leave extracts of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.

scholarly journals PHENOLIC COMPOUND FROM THE STEM BARK OF MANGGIS HUTAN (Garcinia bancana Miq.) AND THEIR ANTIOXIDANT ACTIVITY

2010 ◽  
Vol 9 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Muharni Muharni ◽  
Supriyatna Supriyatna ◽  
Husein H. Bahti ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Spectroscopic Data ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Reported Data

A phenolic compoud, (-)-epicatechin, was isolated from  the stem bark of Garcinia bancana. The structure of this compound was determined base on spectroscopic  data such as including UV, IR, 1-D, 2-D NMR, and comparison with the reported data. Biological activity of this compound at free radical scavenging activity by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and inhibitory xanthine oxidase(XO) activity showed that (-)- epicathechin active at two methods with IC50 value 8.1 and 8.6 mg/mL respectively.   Keywords: phenolic, (-)-epicatechin, Garcinia  bancana, DPPH, XO

scholarly journals ANTIRADICAL ACTIVITY OF 1,4-AND 1,2-BENZENE DICARBOXYL ESTERS FROM DEINBOLLIA PINNATA LEAVES

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
Y. Rufai ◽  
N. Basar ◽  
S. Chandren ◽  
K. Suleiman ◽  
I. Yinusa
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Isomeric Mixture ◽  
Isolation And Purification ◽  
Methanolic Extracts ◽  
Ic50 Value ◽  
Successful Separation ◽  
Dpph Scavenging

The family Sapindaceae are tropical and sub-tropical continental plants. In this study, 1,4-Benzene dicarboxyl ester (1) and 1,2-Benzene dicarboxyl ester (2) from methanolic extracts of Deinbollia pinnata leaves. The successful separation of the isomeric mixture is by freezing the sub-fraction with methanol for seventy-two hours; followed by filtration, isolation and purification. The compounds were characterized spectroscopically (1H &13C NMR, FTIR and GC-MS). The methanolic extracts showed good DPPH scavenging activity with percentage inhibition greater than 70% at 125 ppm, an excellent antioxidant activity towards ABTS assay with the IC50 value of 13.68 µg/mL and also exhibits most potent ferric ion reducer at 3.45 ± 1.30 mM FRAP equivalent compared to other extracts. The 1,4-Benzene dicarboxyl ester (1) displayed high antioxidant radical scavenging activity towards DPPH assay with IC50 value of (IC50 32.99; 73.491%) and 1,2-Benzene dicarboxyl ester (2) at (IC50 33.99; 71.11%);ABTS activity with SC50 value at 48.81µg/mL; 87.98% (1) and 49.55µg/mL; 83.85% (2) and a good potential as ferric ion reducer ranging from 0.05±0.00 to 1.37±0.02 (1) and 0.16±0.04 to 1.29±0.03 (2) mM FRAP equivalent respectively.

scholarly journals Assesment of free radical scavenging activity and inhibitory properties of aqueous extract of Cymodocea serrulata segrass towards α- glucosidase and α –amylase enzyme

2021 ◽  
Vol 23 (12) ◽  
pp. 339-360
Author(s):  
Suresh kumar Tirupur Venkatachalam ◽  
◽  
SenthilKumar Palanisamy ◽  
Sudhagar Babu ◽  
Ranjithkumar Rajamani ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cymodocea Serrulata

Aim: The study reports the in vitro free radical scavenging and α-amylase and α-glucosidase inhibition activities of aqueous extract of seagrass Cymodocea serrulata from Gulf of Mannar, Southeast Asia. Methods: The free radical scavenging activity was evaluated by DPPH, Superoxide, Nitric oxide and Hydroxyl radical scavenging assays and α-amylase, α-glucosidase enzyme inhibitory activity. The C. serrulata showed effective DPPH radical scavenging activity of 69.45 μg/mL, Superoxide radical scavenging activity of 91.08.5 ± 0.02%, Nitric oxide radical scavenging activity of 86.89 ± 0.03% and Hydroxyl radical scavenging activity of 81.09 ± 0.3 % at a concentration of 5 μg/mL respectively. It also showed significant inhibitory activity against α- amylase with an IC50 value of 3.0 ± 0.04 (μg/ml) and the α-glucosidase inhibitory activity with an IC50 value of 3.7 ± 1.01 (μg/ml) at concentrations of 1-5 μg/mL. Conclusion: Cymodocea serrulata was found to be a potent free radical scavenger and inhibitor of α-amylase and α-glucosidase enzymes.

Antioxidant p-terphenyl compound, isolated from edible mushroom, Boletopsis leucomelas

2021 ◽  
Author(s):  
Ferry Ferdiansyah Sofian ◽  
Nodoka Kikuchi ◽  
Takuya Koseki ◽  
Yuko Kanno ◽  
Shota Uesugi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Spectral Analysis ◽  
Free Radical ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Edible Mushroom ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Scavenging

Abstract A new acetyl p-terphenyl derivative, boletopsin 15, was isolated from the ethyl acetate extract of fruit bodies of the Basidiomycete Boletopsis leucomelas, together with four known compounds. The structures of these compounds were elucidated by spectral analysis, as well as by directly comparing the spectral data of the new compound with those of known compounds. The free radical-scavenging activity of the compounds was assayed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The results showed that compounds 1 and 2 exhibited significant antioxidant activity (1: EC50 = 2.1 μM and 2: EC50 = 6.6 μM).

scholarly journals Ethno-Medicinal Survey of Plants Used in Fever and Biological Activity of Selected Medicinal Plants

2019 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
Sunila Paudel ◽  
Khem Raj Joshi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Traditional Treatment ◽  
Ic50 Value ◽  
Keywords Fever

Fifty one plant species belonging to 35 families were documented for the traditional treatment of fever. The widely used species are Centellaasiatica (50%), Ocimumsantum (22.5%), Cissampelospareira (19.5%), Rubusellipticus (10%), and Allium hypsistum (14%). Leaves were used in majority of cases followed by roots in the form of decoction, juice, infusion with or without solvents such as water. Leave extract of R. ellipticus showed potent antioxidant activity towards 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity with IC50 value 2.98 µg/ml compared with standard Ascorbic acid (4.77 µg/ ml). The antibacterial activity of the leave of R. ellipticus showed comparable zone of inhibition with that of standard drugs at a concentration of 1 mg/ml. Keywords: Fever, Antioxidant, Antimicrobial activity, Rubusellipticus, Centellaasiatica

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