scholarly journals Aktivitas Antioksidan dan Evaluasi Sensoris Teh Herbal Bunga Gumitir (Tagetes erecta L.)

2020 ◽  
Vol 5 (2) ◽  
pp. 39
Author(s):  
I Gusti Ngurah Bagus Pranantha Bistara Kusuma ◽  
Ni Komang Ayu Nila Ratna ◽  
Auriel Gabriella Kalalinggi ◽  
I Wayan Rai Widarta
Keyword(s):  
Water Content ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Bioactive Components ◽  
Flavonoid Content ◽  
Herbal Tea ◽  
Tagetes Erecta L

Bunga gumitir memiliki manfaat yang baik bagi kesehatan karena mengandung komponen bioaktif berupa flavonoid, fenol, dan karotenoid berupa lutein sehingga sangat berpotensi digunakan sebagai teh herbal. Penelitian ini bertujuan untuk mendapatkan teknik pengeringan teh herbal bunga gumitir yang tepat sehingga menghasilkan karakteristik teh herbal bunga gumitir dengan komponen bioaktif dan antioksidan yang tinggi serta sensoris yang baik. Pengeringan dilakukan dengan 5 teknik yang berbeda yaitu pengeringan matahari, pengeringan oven, pengeringan dingin, pengeringan udara, dan pengeringan sangrai. Parameter yang diamati meliputi kadar air, total fenol, total flavonoid, aktivitas antioksidan (IC50) serta  pengujian  sensoris air seduhan teh herbal bunga gumitir (warna, aroma, rasa, dan penerimaan keseluruhan). Hasil penelitian menunjukkan bahwa pengeringan dingin mampu menghasilkan teh herbal dengan karakteristik terbaik yaitu kadar air sebesar 6,86%, total fenol sebesar 83,88 mg GAE/g ekstrak, total flavonoid sebesar 373,06 mg QE/g ekstrak, nilai IC50 sebesar 257,65 mg/L, karakteristik sensoris paling disukai dengan rasa agak khas bunga gumitir, rasa tidak asam, dan berwarna merah kekuningan.   Gumitir flower had good benefits for health because their bioactive components contain flavonoids, phenols, and carotenoids like lutein so that gumitir flower were potentially used as herbal tea. The research was conducted to obtain the appropriate gumitir flower tea drying technique to produced the characteristics of gumitir herbal tea with high bioactive and antioxidant components and good sensory. Drying were carried out with 5 different techniques, namely sun drying, oven drying, cold drying, air drying, and roasting drying. Parameters observed included water content, total phenol content, total flavonoid content, antioxidant activity (IC50), and sensory testing of gumitir flower herbal tea (color, aroma, taste, and overall reception). The results showed that cold drying was able to produced herbal tea with the best characteristics with water content was 6.86%, total phenol content was 83.88 mg GAE/g extract, total flavonoid content was 373.06 mg QE/g extract, IC50 was 257.65 mg/L, sensory characteristics were most preferred with a rather typical taste of gumitir flower, not sour and yellowish red.

scholarly journals Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

2018 ◽  
Vol 20 (3) ◽  
pp. 541-550 ◽  
Keyword(s):  
Heavy Metal ◽  
Castanea Sativa ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

scholarly journals Antioxidant properties of Rubus discolor leaf extracts

2016 ◽  
pp. 189-196 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Petar Marin
Keyword(s):  
Antioxidant Properties ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Aqueous Extracts ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Dry Extract

In this work were examined aqueous, methanol, ethanol and acetone leaf extracts of Rubus discolor, wild growing blackberry, for their antioxidant properties and total phenol and flavonoid content. The total phenol content (TPC) varried from 250.05 to 446.61 mg GAE/g of dry extract, while total flavonoid content (TFC) was in range between 22.44 and 61.15 mg QE/g of dry extract. Aqueous extracts were the richest in phenols, as well as in flavonoids. In vitro antioxidant capacity of leaf extracts was evaluated by 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging procedures and ferric reducing ability of plasma (FRAP) assay. Aqueous extracts were the most effective through all antioxidant tests. The total phenol content highly correlated with antioxidant activity of extracts. Moreover, weak correlation was established between total phenol and total flavonoid content. The results presented in this work indicate that phenol compounds contribute to antioxidant ability of extracts.

scholarly journals Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

2014 ◽  
Vol 22 (3) ◽  
pp. 296-302 ◽  
Author(s):  
Quy Diem Do ◽  
Artik Elisa Angkawijaya ◽  
Phuong Lan Tran-Nguyen ◽  
Lien Huong Huynh ◽  
Felycia Edi Soetaredjo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Extraction Solvent

Phytochemicals and Antioxidant Activities of Aloe Vera (Aloe Barbadensis)

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Rojina Bista ◽  
Arjun Ghimire ◽  
Sadikshya Subedi
Keyword(s):  
Aloe Vera ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Constant Weight ◽  
Soxhlet Apparatus

Fresh Aloe vera leaves were collected from Itahari, Sunsari district Nepal and were well washed with distilled water and subjected to cabinet drying at 50°C until constant weight of sample was obtained. Thus, obtained dry powder was extracted using Soxhlet apparatus in two different solvents i.e. methanol and ethanol and further concentrated using rotatory vacuum evaporator that was used for Total Phenol Content (TPC), Total Flavonoid Content (TFC) and tannin content determination. The mean values of methanolic and ethanolic extract were then statistically analyzed at 5% level of significance by paired t-test. Fresh Aloe vera leaves were extracted in 96% methanol to determine chlorophyll-a, chlorophyll- b and total carotene content. Similarly, 99% methanol was used to determine Total Antioxidant Capacity (TOAC), DPPH radical scavenging activity and reducing power assay of fresh Aloe vera leaves. Keywords: Aloe-Vera; Total Phenol Content; Total Flavonoid Content; Total Tannin Content; Antioxidant Activity

Effect of Grape (Vitis vinifera) Seed Extract on the Physico-Chemical, Microbial and Sensory Characteristics of Chevon Nuggets

2020 ◽  
Author(s):  
Priyanka Meena ◽  
Anurag Pandey ◽  
Ashish Saini ◽  
Asman Singh Gurjar ◽  
Ravi Raman ◽  
...  
Keyword(s):  
Frozen Storage ◽  
Seed Extract ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Antioxidant Ability ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Physico Chemical

The present study was carried out to evaluate the antioxidant potential of grape seed extract (GSE) and its effect on the physico-chemical, microbiological and sensory properties of chevon nuggets during frozen storage. Antioxidant potential by various biochemical tests (Antioxidant ability assays, total phenol content, total flavonoid content) were evaluated. The result showed that GSE exhibit high antioxidant ability assays (312.06±1.69 ìg ascorbic acid), total phenol content (190.34±2.26 mg GAE/g), total flavonoid content (83.43±2.36 mg rutin/g). The chevon nugget treated with GSE had significantly (P less than 0.05) lower thiobarbituric acid reactive substance (TBARS) and free fatty acid % (FFA) compared to control during frozen storage. Addition of GSE significantly (P less than 0.05) reduced the total plate, total psychrophilic and yeast and mold count in chevon nuggets during storage at -18±2oC. The GSE treated chevon nuggets recorded significantly (P less than 0.05) superior score of flavor, juiciness and overall acceptability than control. It can be concluded that GSE has excellent antioxidant properties, could be used as an antioxidant to meat products without affecting its quality and acceptability.

scholarly journals Polyphenol contents and free radical scavenging activity of Pinus merkusii cone

2021 ◽  
Vol 891 (1) ◽  
pp. 012017
Author(s):  
Masendra ◽  
A Ngadianto ◽  
R Arifriana ◽  
F Maulidia ◽  
W Minorita ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Soluble Extract ◽  
Pinus Merkusii

Abstract This study aimed to investigate the polyphenol contents from the Pinus merkusii cone extract and their antioxidant activity. The cone powder (40 mesh) was extracted with n-hexane, ethyl acetate, and methanol successively for six hours. The cone of n-hexane, ethyl acetate, and methanol soluble extracts was subjected to total tannin content, total phenol content, total flavonoid content, and antioxidant measurements. The polyphenol contents were measured through the colorimetric method, while antioxidant activity was observed by inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results showed that total tannin content, total phenol content, total flavonoid content, and antioxidant activity of methanol soluble extract was higher than ethyl acetate and n-hexane soluble extracts. In this study, the high antioxidant activity of methanol soluble extract (IC50 of 196.73±13.93 ppm) might due to the high concentration of total phenol content (545.38±54.07 mg GAE/g sample). It was suggested that the P. merkusii cone is potential as the antioxidant source.

scholarly journals EFFECT OF METHANOL CONCENTRATION AS A SOLVENT ON TOTAL PHENOLIC AND FLAVONOID CONTENT OF BELUNTAS LEAF EXTRACT (Pulchea indica L.)

2020 ◽  
Vol 2 (2) ◽  
pp. 77
Author(s):  
Ni Putu Sinta Mahasuari ◽  
N. L. P. Vidya Paramita ◽  
A.A Gede Rai Yadnya Putra
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Total Phenol ◽  
Methanol Concentration ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Flavonoid Content ◽  
Caffeoylquinic Acid ◽  
Phenolic And Flavonoid

Background: Beluntas (Pluchea indica L.) is an Indonesian plant that grows wild and is used as traditional medicine. Beluntas leaves are reported to contain phenolic, and flavonoid is a part of phenolic compounds. Phenolic compounds other than flavonoids include 1,3,4,5-tetra-O-caffeoylquinic acid, 3,4,5-tri-O-caffeoylquinic acid, chlorogenic acid, and ferulic acid. Flavonoid compounds in beluntas leaves are quercetin, apigenin, luteolin and chrysoeriol. Methanol solvents are reported to be able to extract higher polyphenol and flavonoid contents than other solvents. Objective: The purpose of this study is to determine the effect of 20%, 50% and 75% solvent concentration of methanol on total phenol and total flavonoid levels of beluntas leaf extract. Methods: In this study, the extraction process was carried out by maceration, the determination of total phenol content was carried out by the Follin-Ciocalteu method. Determination of total flavonoid levels was carried out by the Colorimetric method. Data on phenol and flavonoid levels were analyzed statistically. Results: The yield of beluntas leaf extract in this study was respectively from the lowest methanol concentration of 24.094% w/w, 31.126% w/w, 24.838% w/w. The value of total phenol levels increased with increasing methanol concentration, namely 124.84 mg GAE/g, 138.3 mg GAE/g, and 147.91 mg GAE/g. The highest total flavonoid value in 75% methanol extract is 69.72 mg QE/g, followed by 20% methanol extract at 46.29 mg QE/g and the lowest is found in 50% methanol extract at 32.80 mg QE/g. The results of statistical analysis using the Kruskal-Wallis test showed that there were significant differences (p<0.05) in the value of total phenol levels and total flavonoids of the three extracts. Conclusion: The difference in the concentration of methanol solvents affects the value of total phenol levels and total flavonoids with the highest value produced by a 75% methanol solvent. Keywords: Beluntas Leaves (Pluchea indica L.), Total Phenol Content, Total Flavonoid Content, Gallic Acid, Quercetin.

scholarly journals Antioxidant Activity of Ketapang Leaf Tea (Terminalia catappa)

2021 ◽  
Vol 4 (2) ◽  
pp. 220-227
Author(s):  
Retno Widyastuti ◽  
Agustina Intan Niken Tari ◽  
Novian Wely Asmoro
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Leaf Age ◽  
Black Tea ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Terminalia Catappa ◽  
Young Leaves

The purpose of this study was to determine the antioxidant activity of fermented tea (black tea) with Ketapang leaves (Terminalia catappa) using two variations of leaf age, namely leaflets 1-3 and 4-5. The parameters observed were water content, pH, antioxidant activity (IC 50) and total phenol content of tea. Results showed that the antioxidant activity of young leaves (shoots 1-3) significantly higher than old leaves (shoots 4-5) but had lower total phenol and pH. In general, the character of Ketapang leaf tea products at shoots 1-5 has water content ranging from 5.55-5.58%, total phenol content of 22.63-28.97 mg / g EAG, and antioxidant activity (IC 50) of 6.27-6 .71 µg/ml and the pH of steeping water from 5.9 to 6.1. 

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