scholarly journals Polyphenol contents and free radical scavenging activity of Pinus merkusii cone

2021 ◽  
Vol 891 (1) ◽  
pp. 012017
Author(s):  
Masendra ◽  
A Ngadianto ◽  
R Arifriana ◽  
F Maulidia ◽  
W Minorita ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Soluble Extract ◽  
Pinus Merkusii

Abstract This study aimed to investigate the polyphenol contents from the Pinus merkusii cone extract and their antioxidant activity. The cone powder (40 mesh) was extracted with n-hexane, ethyl acetate, and methanol successively for six hours. The cone of n-hexane, ethyl acetate, and methanol soluble extracts was subjected to total tannin content, total phenol content, total flavonoid content, and antioxidant measurements. The polyphenol contents were measured through the colorimetric method, while antioxidant activity was observed by inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results showed that total tannin content, total phenol content, total flavonoid content, and antioxidant activity of methanol soluble extract was higher than ethyl acetate and n-hexane soluble extracts. In this study, the high antioxidant activity of methanol soluble extract (IC50 of 196.73±13.93 ppm) might due to the high concentration of total phenol content (545.38±54.07 mg GAE/g sample). It was suggested that the P. merkusii cone is potential as the antioxidant source.

scholarly journals Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

2014 ◽  
Vol 22 (3) ◽  
pp. 296-302 ◽  
Author(s):  
Quy Diem Do ◽  
Artik Elisa Angkawijaya ◽  
Phuong Lan Tran-Nguyen ◽  
Lien Huong Huynh ◽  
Felycia Edi Soetaredjo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Extraction Solvent

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ECENG PADI (Monochoria vaginalis Burm F. C. Presel.)

2020 ◽  
Vol 9 (1) ◽  
pp. 81
Author(s):  
Ida Ayu Gede Padmawati ◽  
I Ketut Suter ◽  
Ni Made Indri Hapsari Arihantana
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of rice hyacinth extract and to find out the right type of solvent used to extract rice hyacinth with the highest antioxidant activity. The experimental design used in this study was a completely randomized design with various types of solvents as a treatment using maceration method. The treatment consisting of four type of solvents i.e water, ethanol, methanol, and acetone. All treatments were repeated four times to obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect then followed by Duncan test. The results showed that the various types of solvent treatments had a very significant effect (P<0.01) on antioxidant activity. The results showed that ethanol solvent had the best treatment which produced an antioxidant activity based on IC50 of 0,49 mg/mL, yield of 26.17%, total phenol content of 11.12 mg GAE/g, total flavonoid content of 8.26 mg QE/g, total tannin content of 5.18 mg TAE/g. Keywords: rice hyacinth, maceration, anioxidant activity

Phytochemicals and Antioxidant Activities of Aloe Vera (Aloe Barbadensis)

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Rojina Bista ◽  
Arjun Ghimire ◽  
Sadikshya Subedi
Keyword(s):  
Aloe Vera ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Constant Weight ◽  
Soxhlet Apparatus

Fresh Aloe vera leaves were collected from Itahari, Sunsari district Nepal and were well washed with distilled water and subjected to cabinet drying at 50°C until constant weight of sample was obtained. Thus, obtained dry powder was extracted using Soxhlet apparatus in two different solvents i.e. methanol and ethanol and further concentrated using rotatory vacuum evaporator that was used for Total Phenol Content (TPC), Total Flavonoid Content (TFC) and tannin content determination. The mean values of methanolic and ethanolic extract were then statistically analyzed at 5% level of significance by paired t-test. Fresh Aloe vera leaves were extracted in 96% methanol to determine chlorophyll-a, chlorophyll- b and total carotene content. Similarly, 99% methanol was used to determine Total Antioxidant Capacity (TOAC), DPPH radical scavenging activity and reducing power assay of fresh Aloe vera leaves. Keywords: Aloe-Vera; Total Phenol Content; Total Flavonoid Content; Total Tannin Content; Antioxidant Activity

scholarly journals Aktivitas Antioksidan dan Evaluasi Sensoris Teh Herbal Bunga Gumitir (Tagetes erecta L.)

2020 ◽  
Vol 5 (2) ◽  
pp. 39
Author(s):  
I Gusti Ngurah Bagus Pranantha Bistara Kusuma ◽  
Ni Komang Ayu Nila Ratna ◽  
Auriel Gabriella Kalalinggi ◽  
I Wayan Rai Widarta
Keyword(s):  
Water Content ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Bioactive Components ◽  
Flavonoid Content ◽  
Herbal Tea ◽  
Tagetes Erecta L

Bunga gumitir memiliki manfaat yang baik bagi kesehatan karena mengandung komponen bioaktif berupa flavonoid, fenol, dan karotenoid berupa lutein sehingga sangat berpotensi digunakan sebagai teh herbal. Penelitian ini bertujuan untuk mendapatkan teknik pengeringan teh herbal bunga gumitir yang tepat sehingga menghasilkan karakteristik teh herbal bunga gumitir dengan komponen bioaktif dan antioksidan yang tinggi serta sensoris yang baik. Pengeringan dilakukan dengan 5 teknik yang berbeda yaitu pengeringan matahari, pengeringan oven, pengeringan dingin, pengeringan udara, dan pengeringan sangrai. Parameter yang diamati meliputi kadar air, total fenol, total flavonoid, aktivitas antioksidan (IC50) serta  pengujian  sensoris air seduhan teh herbal bunga gumitir (warna, aroma, rasa, dan penerimaan keseluruhan). Hasil penelitian menunjukkan bahwa pengeringan dingin mampu menghasilkan teh herbal dengan karakteristik terbaik yaitu kadar air sebesar 6,86%, total fenol sebesar 83,88 mg GAE/g ekstrak, total flavonoid sebesar 373,06 mg QE/g ekstrak, nilai IC50 sebesar 257,65 mg/L, karakteristik sensoris paling disukai dengan rasa agak khas bunga gumitir, rasa tidak asam, dan berwarna merah kekuningan.   Gumitir flower had good benefits for health because their bioactive components contain flavonoids, phenols, and carotenoids like lutein so that gumitir flower were potentially used as herbal tea. The research was conducted to obtain the appropriate gumitir flower tea drying technique to produced the characteristics of gumitir herbal tea with high bioactive and antioxidant components and good sensory. Drying were carried out with 5 different techniques, namely sun drying, oven drying, cold drying, air drying, and roasting drying. Parameters observed included water content, total phenol content, total flavonoid content, antioxidant activity (IC50), and sensory testing of gumitir flower herbal tea (color, aroma, taste, and overall reception). The results showed that cold drying was able to produced herbal tea with the best characteristics with water content was 6.86%, total phenol content was 83.88 mg GAE/g extract, total flavonoid content was 373.06 mg QE/g extract, IC50 was 257.65 mg/L, sensory characteristics were most preferred with a rather typical taste of gumitir flower, not sour and yellowish red.

scholarly journals Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

2018 ◽  
Vol 20 (3) ◽  
pp. 541-550 ◽  
Keyword(s):  
Heavy Metal ◽  
Castanea Sativa ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

scholarly journals Total Phenolics, Flavonoids Contents, Antioxidant Activity and DNA Protective Effect of Lenten Rose (Helleborus orientalis)

2019 ◽  
pp. 1-12
Author(s):  
Meral Öztürk ◽  
İbrahim Bulduk ◽  
Safiye Elif Korcan ◽  
Recep Liman ◽  
Funda Karabağ Çoban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Leaf Extract ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Helleborus Orientalis ◽  
Different Parts

The aims of the present study were to evaluate the antioxidant activity and DNA Protective effect of Helleborus orientalis (HO) leaf extract against oxidative damage, and to determine the total phenolic and flavonoid contents of the plant species studied. Methods: The total phenol content (TPC) of H. orientalis (Ranunculaceae) extract was determined using the Folin-Ciocalteu technique. The aluminum chloride colorimetric assay in the determination of The total flavonoid content (TFC) and was used, Analysis of Phenolic Acids was identified by High-Performance Liquid Chromatography (HPLC). Antioxidant activity was analyzed by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Assay. Protective effect of H. orientalis leaf extract against to H2O2 was evaluated by using TAS, TOS methods and Comet assay. Key Findings: H. orientalis ethanol extracts contain high amounts of antioxidants. The HO leaf methanol extract (LME) decreased the DNA damage at all tested concentrations in a dose-dependent manner (r=0.86 p<0.01) against to H2O2. Conclusions: The total phenol content in the extracts of different parts of the plant varied from 4.00 mg GAE/1 gr to 19.42 mg GAE/1 gr. The flowers had the highest phenol content (19.42 mg GAE/1 gr sample) and followed by the leaves (17.20 mg GAE/1 gr sample). The total flavonoid content in the extracts from different parts of the plant varied from 2.57 mg QE/1 gr to 11.88 mg QE/1 gr. The flowers had the highest flavonoid content (11.88 mg QE/1 gr sample) and followed by the leaves (10.21 mg QE/1 gr sample). Antioxidant activity of fractions was explained as a percentage of DPPH radicals’ scavenging and IC50 values (μg/ml). Leaf and flowers of HO are richer in antioxidant than its root and stem. As the concentration of leaf extracts used increased, the DNA protective effect increased and it was statistically significant at overdoses of 2500 μg/mL. Total antioxidant status (TAS) levels were significantly (p <0.05) decreased in the H2O2 group (3.4±0.21) but H. orientalis was significantly (p<0.05) increased TAS levels in this group. When the concentration of leaf extracts used increased, the protective effect has also increased and statistically significant at overdoses of 2500 μg / mL (6.3±0.67). Total oxidant status (TOS) levels were significantly (p <0.05) increased in the H2O2 group (25.3±0.74) and H. orientalis was significantly (p<0.05) decreased TOS levels in groups.

scholarly journals Antioxidant properties of Rubus discolor leaf extracts

2016 ◽  
pp. 189-196 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Petar Marin
Keyword(s):  
Antioxidant Properties ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Aqueous Extracts ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Dry Extract

In this work were examined aqueous, methanol, ethanol and acetone leaf extracts of Rubus discolor, wild growing blackberry, for their antioxidant properties and total phenol and flavonoid content. The total phenol content (TPC) varried from 250.05 to 446.61 mg GAE/g of dry extract, while total flavonoid content (TFC) was in range between 22.44 and 61.15 mg QE/g of dry extract. Aqueous extracts were the richest in phenols, as well as in flavonoids. In vitro antioxidant capacity of leaf extracts was evaluated by 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging procedures and ferric reducing ability of plasma (FRAP) assay. Aqueous extracts were the most effective through all antioxidant tests. The total phenol content highly correlated with antioxidant activity of extracts. Moreover, weak correlation was established between total phenol and total flavonoid content. The results presented in this work indicate that phenol compounds contribute to antioxidant ability of extracts.

scholarly journals DETERMINATION OF PHYSICOCHEMICAL PROPERTIES, ANTIOXIDANT CONSTITUENTS BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FINGERPRINTING, AND ANTIOXIDANT ACTIVITY OF CUCURBITA MAXIMA SEEDS

2018 ◽  
Vol 11 (3) ◽  
pp. 280
Author(s):  
Pinki Pal ◽  
Shiv Bhadra Singh ◽  
Aaditya Singh
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Layer Chromatography

 Objective: The present study is designed to evaluate preliminary phytochemical constituents, physiochemical properties evaluation, free radical scavenging activity, and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis of Cucurbita maxima seed oil.Methods: Total phenol content, total flavonoid content, total tannin content, 1, 1-diphenyl, 2-picryl-hydrazyl (DPPH) radical scavenging method, and HPTLC fingerprinting analysis for compounds responsible for antioxidant activity were used.Result: Phytochemical screening shows the presence of constituents such as alkaloids, glycosides, carbohydrates, fixed oil and fats, phytosterols, saponin, phenolic compounds, tannins, proteins, amino acids, gums, and mucilage. Physicochemical studies show that the oil contains an acid value, saponification value, iodine value of 9.53 mg KOH/g, 183 mg KOH/g, and 116.51 mg I2/100 g, respectively. Total phenol content was 3.9498±3.2 mg gallic acid equivalent/g, total flavonoid content was 56.115±4.6 mg rutin equivalent/g, and total tannin content was 19.4±0.40 mg tannic acid equivalent/g. The radical scavenging activity by DPPH Model shows that the methanolic extract has maximum antioxidant activity with IC50 value of 238.31. HPTLC fingerprinting also proofs the presence of phenol and flavonoid compounds in the seed oil.Conclusion: The results in the paper show that the C. maxima seed oil is an natural antioxidant and can be used for the treatment of various disorders.

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