Phytochemicals and Antioxidant Activities of Aloe Vera (Aloe Barbadensis)

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Rojina Bista ◽  
Arjun Ghimire ◽  
Sadikshya Subedi
Keyword(s):  
Aloe Vera ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Constant Weight ◽  
Soxhlet Apparatus

Fresh Aloe vera leaves were collected from Itahari, Sunsari district Nepal and were well washed with distilled water and subjected to cabinet drying at 50°C until constant weight of sample was obtained. Thus, obtained dry powder was extracted using Soxhlet apparatus in two different solvents i.e. methanol and ethanol and further concentrated using rotatory vacuum evaporator that was used for Total Phenol Content (TPC), Total Flavonoid Content (TFC) and tannin content determination. The mean values of methanolic and ethanolic extract were then statistically analyzed at 5% level of significance by paired t-test. Fresh Aloe vera leaves were extracted in 96% methanol to determine chlorophyll-a, chlorophyll- b and total carotene content. Similarly, 99% methanol was used to determine Total Antioxidant Capacity (TOAC), DPPH radical scavenging activity and reducing power assay of fresh Aloe vera leaves. Keywords: Aloe-Vera; Total Phenol Content; Total Flavonoid Content; Total Tannin Content; Antioxidant Activity

scholarly journals Aktivitas Antioksidan dan Evaluasi Sensoris Teh Herbal Bunga Gumitir (Tagetes erecta L.)

2020 ◽  
Vol 5 (2) ◽  
pp. 39
Author(s):  
I Gusti Ngurah Bagus Pranantha Bistara Kusuma ◽  
Ni Komang Ayu Nila Ratna ◽  
Auriel Gabriella Kalalinggi ◽  
I Wayan Rai Widarta
Keyword(s):  
Water Content ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Bioactive Components ◽  
Flavonoid Content ◽  
Herbal Tea ◽  
Tagetes Erecta L

Bunga gumitir memiliki manfaat yang baik bagi kesehatan karena mengandung komponen bioaktif berupa flavonoid, fenol, dan karotenoid berupa lutein sehingga sangat berpotensi digunakan sebagai teh herbal. Penelitian ini bertujuan untuk mendapatkan teknik pengeringan teh herbal bunga gumitir yang tepat sehingga menghasilkan karakteristik teh herbal bunga gumitir dengan komponen bioaktif dan antioksidan yang tinggi serta sensoris yang baik. Pengeringan dilakukan dengan 5 teknik yang berbeda yaitu pengeringan matahari, pengeringan oven, pengeringan dingin, pengeringan udara, dan pengeringan sangrai. Parameter yang diamati meliputi kadar air, total fenol, total flavonoid, aktivitas antioksidan (IC50) serta  pengujian  sensoris air seduhan teh herbal bunga gumitir (warna, aroma, rasa, dan penerimaan keseluruhan). Hasil penelitian menunjukkan bahwa pengeringan dingin mampu menghasilkan teh herbal dengan karakteristik terbaik yaitu kadar air sebesar 6,86%, total fenol sebesar 83,88 mg GAE/g ekstrak, total flavonoid sebesar 373,06 mg QE/g ekstrak, nilai IC50 sebesar 257,65 mg/L, karakteristik sensoris paling disukai dengan rasa agak khas bunga gumitir, rasa tidak asam, dan berwarna merah kekuningan.   Gumitir flower had good benefits for health because their bioactive components contain flavonoids, phenols, and carotenoids like lutein so that gumitir flower were potentially used as herbal tea. The research was conducted to obtain the appropriate gumitir flower tea drying technique to produced the characteristics of gumitir herbal tea with high bioactive and antioxidant components and good sensory. Drying were carried out with 5 different techniques, namely sun drying, oven drying, cold drying, air drying, and roasting drying. Parameters observed included water content, total phenol content, total flavonoid content, antioxidant activity (IC50), and sensory testing of gumitir flower herbal tea (color, aroma, taste, and overall reception). The results showed that cold drying was able to produced herbal tea with the best characteristics with water content was 6.86%, total phenol content was 83.88 mg GAE/g extract, total flavonoid content was 373.06 mg QE/g extract, IC50 was 257.65 mg/L, sensory characteristics were most preferred with a rather typical taste of gumitir flower, not sour and yellowish red.

scholarly journals Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

2018 ◽  
Vol 20 (3) ◽  
pp. 541-550 ◽  
Keyword(s):  
Heavy Metal ◽  
Castanea Sativa ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

scholarly journals Antioxidant properties of Rubus discolor leaf extracts

2016 ◽  
pp. 189-196 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Petar Marin
Keyword(s):  
Antioxidant Properties ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Aqueous Extracts ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Dry Extract

In this work were examined aqueous, methanol, ethanol and acetone leaf extracts of Rubus discolor, wild growing blackberry, for their antioxidant properties and total phenol and flavonoid content. The total phenol content (TPC) varried from 250.05 to 446.61 mg GAE/g of dry extract, while total flavonoid content (TFC) was in range between 22.44 and 61.15 mg QE/g of dry extract. Aqueous extracts were the richest in phenols, as well as in flavonoids. In vitro antioxidant capacity of leaf extracts was evaluated by 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging procedures and ferric reducing ability of plasma (FRAP) assay. Aqueous extracts were the most effective through all antioxidant tests. The total phenol content highly correlated with antioxidant activity of extracts. Moreover, weak correlation was established between total phenol and total flavonoid content. The results presented in this work indicate that phenol compounds contribute to antioxidant ability of extracts.

scholarly journals Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

2014 ◽  
Vol 22 (3) ◽  
pp. 296-302 ◽  
Author(s):  
Quy Diem Do ◽  
Artik Elisa Angkawijaya ◽  
Phuong Lan Tran-Nguyen ◽  
Lien Huong Huynh ◽  
Felycia Edi Soetaredjo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Extraction Solvent

scholarly journals Polyphenol contents and free radical scavenging activity of Pinus merkusii cone

2021 ◽  
Vol 891 (1) ◽  
pp. 012017
Author(s):  
Masendra ◽  
A Ngadianto ◽  
R Arifriana ◽  
F Maulidia ◽  
W Minorita ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Soluble Extract ◽  
Pinus Merkusii

Abstract This study aimed to investigate the polyphenol contents from the Pinus merkusii cone extract and their antioxidant activity. The cone powder (40 mesh) was extracted with n-hexane, ethyl acetate, and methanol successively for six hours. The cone of n-hexane, ethyl acetate, and methanol soluble extracts was subjected to total tannin content, total phenol content, total flavonoid content, and antioxidant measurements. The polyphenol contents were measured through the colorimetric method, while antioxidant activity was observed by inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results showed that total tannin content, total phenol content, total flavonoid content, and antioxidant activity of methanol soluble extract was higher than ethyl acetate and n-hexane soluble extracts. In this study, the high antioxidant activity of methanol soluble extract (IC50 of 196.73±13.93 ppm) might due to the high concentration of total phenol content (545.38±54.07 mg GAE/g sample). It was suggested that the P. merkusii cone is potential as the antioxidant source.

Effect of Grape (Vitis vinifera) Seed Extract on the Physico-Chemical, Microbial and Sensory Characteristics of Chevon Nuggets

2020 ◽  
Author(s):  
Priyanka Meena ◽  
Anurag Pandey ◽  
Ashish Saini ◽  
Asman Singh Gurjar ◽  
Ravi Raman ◽  
...  
Keyword(s):  
Frozen Storage ◽  
Seed Extract ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Antioxidant Ability ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Physico Chemical

The present study was carried out to evaluate the antioxidant potential of grape seed extract (GSE) and its effect on the physico-chemical, microbiological and sensory properties of chevon nuggets during frozen storage. Antioxidant potential by various biochemical tests (Antioxidant ability assays, total phenol content, total flavonoid content) were evaluated. The result showed that GSE exhibit high antioxidant ability assays (312.06±1.69 ìg ascorbic acid), total phenol content (190.34±2.26 mg GAE/g), total flavonoid content (83.43±2.36 mg rutin/g). The chevon nugget treated with GSE had significantly (P less than 0.05) lower thiobarbituric acid reactive substance (TBARS) and free fatty acid % (FFA) compared to control during frozen storage. Addition of GSE significantly (P less than 0.05) reduced the total plate, total psychrophilic and yeast and mold count in chevon nuggets during storage at -18±2oC. The GSE treated chevon nuggets recorded significantly (P less than 0.05) superior score of flavor, juiciness and overall acceptability than control. It can be concluded that GSE has excellent antioxidant properties, could be used as an antioxidant to meat products without affecting its quality and acceptability.

scholarly journals Effect of extraction solvent on total phenolic content, total flavonoid content and antioxidant activity of Cetraria islandica

2020 ◽  
Vol 13 (3) ◽  
pp. 198-205
Author(s):  
Nataliya Stadnytska ◽  
Iryna Fito ◽  
Volodymyr Novikov ◽  
Izabela Jasicka-Misiak ◽  
Piotr P. Wieczorek
Keyword(s):  
Antioxidant Activity ◽  
Total Flavonoid ◽  
High Rate ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Frap Assay ◽  
Coarse Powder ◽  
Soxhlet Apparatus

Oxidative stress may lead to a number of diseases such as atherosclerosis, nephrotoxicity, liver cirrhosis, cancers, diabetes, and Alzheimer disease. Medicinal plants are an important source of antioxidants. Therefore, the antioxidant potential of Cetraria islandica was evaluated in this work. The coarse powder of leaf of Cetraria islandica was extracted in Soxhlet apparatus, with ethanol (90%) and ethanolic extract of Cetraria islandica (EECI) was further processed for phytochemical screening, total phenol content, total flavonoid content, and various in vitro antioxidant assays. The phytochemicals present in EECI were glycosides, carbohydrates, triterpenoids, proteins and amino acids, gums and mucilages, and flavonoids. The content of extractives was 8.3 mg/ml for Cetraria islandica еxtract 70%. The content of polyphenolic compounds in terms of per head acid was the highest for the sample of 70% and amounted to 0.586 mg/ml, and the content of flavonoids per standard solution quercetin - 0,012 mg/ml - also for 70% extract. By DPPH, the percentage of radicals of absorbing activity for 70% of the extract was 86%, and the antiradical activity of 0,417, indicating a sufficiently high rate of Cetraria islandica antioxidant activity at such a concentration of ethanol. The tested extracts showed next results by FRAP assay: 486 μmol/L (EE96) 135 μmol/L (EE70) and 158 μmol/L (EE40). ABTS method showed the highest result of the extract of Cetraria islandica 40%. The obtained results confirmed the high potential of the extracts as a source of phenolic compounds, in particular flavonoids.

scholarly journals PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ECENG PADI (Monochoria vaginalis Burm F. C. Presel.)

2020 ◽  
Vol 9 (1) ◽  
pp. 81
Author(s):  
Ida Ayu Gede Padmawati ◽  
I Ketut Suter ◽  
Ni Made Indri Hapsari Arihantana
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Tannin Content ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Right

This study was conducted to determine the effect of different solvents on the antioxidant activity of rice hyacinth extract and to find out the right type of solvent used to extract rice hyacinth with the highest antioxidant activity. The experimental design used in this study was a completely randomized design with various types of solvents as a treatment using maceration method. The treatment consisting of four type of solvents i.e water, ethanol, methanol, and acetone. All treatments were repeated four times to obtained 16 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect then followed by Duncan test. The results showed that the various types of solvent treatments had a very significant effect (P<0.01) on antioxidant activity. The results showed that ethanol solvent had the best treatment which produced an antioxidant activity based on IC50 of 0,49 mg/mL, yield of 26.17%, total phenol content of 11.12 mg GAE/g, total flavonoid content of 8.26 mg QE/g, total tannin content of 5.18 mg TAE/g. Keywords: rice hyacinth, maceration, anioxidant activity

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