scholarly journals Evaluation of Micro Tuberization Performances of Different Genotypes

Author(s):  
Ayten Kübra Türkmen ◽  
Caner Yavuz ◽  
Sarbesh Das Dangol ◽  
Cehibe Tarım ◽  
Ufuk Demirel ◽  
...  

Certified potato seed tuber usage is one of the most important steps for production of high yield and quality potatoes. For this reason different seed tuber production methods have been developed. Among these methods, mini tuber production is the most popular one. In order to produce mini tubers, firstly potato plants are produced in vitro, and these plants are transferred to an environmentally-controlled greenhouse. Thus, disease- and virus-free mini tubers are produced as seed tubers. However, in vitro section of mini tuber production creates problems like storage and transfer of in vitro plants, and adaptation period of the plants to greenhouse conditions. In vitro micro tuber (MT) formation has been selected as a solution of these problems. The aim of the study was to produce micro tubers from 15 different genotypes and evaluate their micro tuberization performances to determine the genotype effect on MT formation. 3 varieties, 3 breeding lines and 9 different genotypes from International Potato Center (CIP) were selected for the study. For this purpose, micro tubers are produced in vitro by using Murashige and Skoog (MS) medium supplemented with 8% sucrose and 0.1 mg/L thidiazuron (TDZ). All experiments were conducted under dark conditions and 22/16 °C (8/16 h) temperature cycle. The micro tuberization performances were evaluated according to MT number per plant, MT formation rate (%), MT weight per plant (g), mean MT weight (g), mean MT diameter (mm). Differences between micro tuber production performances of different genotypes were determined and CIP395017.229 was identified as the most promising genotype to produce micro tubers.

2018 ◽  
Vol 41 (14) ◽  
pp. 1765-1777 ◽  
Author(s):  
Ashkan Abbasian ◽  
Ali Ahmadi ◽  
Ali-Reza Abbasi ◽  
Babak Darvishi

1992 ◽  
Vol 40 (1) ◽  
pp. 21-41 ◽  
Author(s):  
W.J.M. Lommen ◽  
P.C. Struik

In vitro-propagated potato cv. Ostara and Bintje plantlets were transplanted in a greenhouse at 350 plants/msuperscript 2 under tuber-inducing conditions. Plants growing undisturbed were compared with plants from which tubers >=0.3 g were removed in a single non-destructive harvest 3-8 weeks after transplanting. In undisturbed plants tuber initiation slowed down 4 weeks after transplanting, and an average of 2 tubers/plant (average weight 5 g) were harvested in 11 weeks. After a non-destructive harvest new stolons and tubers were initiated, but overall and tuber growth rates were reduced, probably as a result of the combined effects of tuber removal, root damage and deep replanting. Highest tuber numbers and lowest growth rate reductions occurred when growth was at its maximum. The highest number of tubers/plant (3.44) was achieved with non-destructive harvesting 6 weeks after transplanting, but FW/tuber decreased with delay in harvesting from 1.97 to 0.77 g. Using this non-destructive harvesting procedure >1400 and 2400 minitubers >=0.3 g (average weight 1-2 g) could be produced per msuperscript 2 within 8 and 9 weeks of transplanting by Ostara and Bintje, respectively, and would be suitable for use in large-scale seed tuber production programmes. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2009 ◽  
Vol 52 (2) ◽  
pp. 121-140 ◽  
Author(s):  
William W. Kirk ◽  
Firas Abu-El Samen ◽  
Pavani Tumbalam ◽  
Phillip Wharton ◽  
David Douches ◽  
...  

1978 ◽  
Vol 26 (4) ◽  
pp. 354-365 ◽  
Author(s):  
J. Marinus ◽  
K.B.A. Bodlaender

The influence of GA on growth and yield of potato seed tubers (size 28-45 mm) was studied in field experiments on sandy soil in the late cv. Alpha. Different GA conc. were applied to seed tubers with or without sprouts by both dipping and spraying. The main purpose was to find a more suitable method of preparing seed tubers than the laborious presprouting in light method. This other method would allow mechanical planting, should not be laborious and should give a high yield of seed tubers. The application of GA accelerated emergence. GA also significantly increased the yield of the seed tuber fraction, probably by increasing the number of stems and thus the number of tubers/plant. GA accelerated tuberization, decreased the total tuber yields in one experiment, whereas in the other experiment an increase occurred. The best results were obtained if sprouted tubers were treated and under favourable growing conditions. Spraying was equally effective as dipping also in the 'spraying just before planting' treatment after heat shock. This means that application during mechanical planting seems to be possible, on the understanding that the GA conc. is not too high to prevent the occurrence of phytotoxic effects (45 mg GA/l caused small leaflets with yellow discolouration). The application of GA to seed tubers seems to be attractive especially in slowly emerging cv. or in cv. with a high proportion of large tubers. (Abstract retrieved from CAB Abstracts by CABI’s permission)


2007 ◽  
Vol 32 (2) ◽  
pp. 1329-1341
Author(s):  
A. Abou El-Yazied ◽  
S. Elminiawy ◽  
M. Al-Deep ◽  
N. Hmmoud

2011 ◽  
Vol XVII (2) ◽  
pp. 173-182
Author(s):  
Román Flores López ◽  
◽  
Felipe Sánchez del Castillo ◽  
Juan Enrique Rodríguez-Pérez ◽  
Rafael Mora Aguilar ◽  
...  

Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 483a-483
Author(s):  
Roy N. Keys ◽  
Dennis T. Ray ◽  
David A. Dierig

Guayule (Parthenium argentatum Gray, Asteraceae) is a latex-producing perennial desert shrub that is potentially of economic importance as an industrial crop for the desert Southwest. It is known to possess complex reproductive modes. Diploids are predominantly sexual and self-incompatible, while polyploids show a range of apomictic potential and self-compatibility. This paper describes the development of a relatively rapid and simple technique for characterizing reproductive modes of breeding lines of P. argentatum. Initial field experiments were based on an auxin test used successfully to characterize reproductive mode in the Poaceae. The application of 2,4-dichlorophenoxyacetic acid inhibited embryo formation in P. argentatum, but this was not the case with other auxins tested. Results of field experiments were ambiguous because: 1) the floral structure of P. argentatum is such that auxins might not have penetrated to the ovules, and 2) there was potential self-fertilization by pollen released within isolation bags. Therefore, in vitro culture of flower heads was tested because it provided much better control of environmental conditions, growth regulator application, and pollen release. Auxin alone, or in combination with gibberellic acid or kinetin, inhibited parthenogenesis in vitro. Embryo production did not vary using two substantially different nutrient media. In vitro flower head culture using a (Nitsch and Nitsch) liquid nutrient medium without growth regulators, enabled characterization of the reproductive mode of seven breeding lines, ranging from predominantly sexual to predominantly apomictic. The results of this technique were substantiated using RAPD analyzes of progeny arrays from controlled crosses.


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