scholarly journals Elisitör Olarak Kullanılan Çinko Sülfatın Biber Kalluslarının Süperoksit Dismutaz, Peroksidaz ve Toplam Fenolik Bileşikleri Üzerine Etkileri

2020 ◽  
Vol 8 (12) ◽  
pp. 2780-2784
Author(s):  
Cemil İşlek ◽  
Bengü Türkyılmaz Ünal ◽  
Sinan Aydın
Keyword(s):  
Cell Culture ◽  
Superoxide Dismutase ◽  
Enzyme Activities ◽  
Zinc Sulphate ◽  
Culture Media ◽  
Cell Suspension Cultures ◽  
Hot Pepper ◽  
Phenolic Substance ◽  
Peroxidase Enzyme

The amount of secondary metabolites can be increased with different elicitor applications in vitro. It has been determined that zinc sulphate significantly increases the amount of capsaicin in the cell culture of red hot pepper. It is important to determine how the metal applied as elicitor will have an effect on plant metabolism. In the study, it was aimed to determine the effects of zinc sulphate (ZnSO4) applied to the cell suspension cultures of pepper seeds at different concentrations (0.1 M, 0.2 M, 0.4 M) and for periods (24, 48, 72 hours) on the total protein and phenolic substance amounts, and superoxide dismutase-peroxidase enzyme activities of pepper calluses. It was observed that the amount of protein increased, superoxide dismutase and peroxidase enzyme activities decreased, and the total amount of phenolic substance increased especially in 72 hours of treatment where zinc was applied as elicitor. These results show that ZnSO4 can be used as an abiotic elicitor in plant cell culture media.

scholarly journals Gümüş Nitrat (AgNO3) Çözeltisinin Capsicum annuum L. Hücre Süspansiyon Kültüründe Toplam Protein, Toplam Fenolik ve Bazı Antioksidant Enzim Aktiviteleri Üzerine Etkisi

2017 ◽  
Vol 5 (12) ◽  
pp. 1544
Author(s):  
Sinan Aydın ◽  
Cemil İşlek ◽  
Bengü Türkyılmaz Ünal
Keyword(s):  
Superoxide Dismutase ◽  
Total Protein ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Total Phenolic ◽  
Antioxidant Enzyme Activities ◽  
Pepper Plant ◽  
Peroxidase Enzyme ◽  
Silver Metal

Heavy metal pollution is a significant environmental problem with negative potential impacts on agriculture and human health. In this study, calluses were obtained by using in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from these calluses. The effect of silver nitrate (AgNO3) solution added in different concentrations and times to cell suspension cultures of pepper on total protein and total phenolic compound amounts, superoxide dismutase and peroxidase enzyme activities were analysed. Total protein and total phenolic amounts, superoxide dismutase and peroxidase enzyme activities were detected by spectrophotometric methods. When the effects of silver metal on pepper plant were examined, it was determined that silver metal reduced the total protein and phenolic content in the pepper plant cells and especially at higher concentration, in the first 24 hour period, antioxidant enzyme activities increased.

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kiptiyah Kiptiyah ◽  
Widodo Widodo ◽  
Gatot Ciptadi ◽  
Aulanni’am Aulanni’Am ◽  
Mohammad A. Widodo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Culture ◽  
Superoxide Dismutase ◽  
In Silico ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Sod Activity ◽  
In Silico Studies ◽  
Incubation Periods

AbstractBackgroundWe investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.MethodsFor the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.ResultsThe in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.Conclusions10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.

scholarly journals Impact of serum in cell culture media on in vitro lactate dehydrogenase (LDH) release determination

2017 ◽  
Vol 3 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Bernhard Hiebl ◽  
Sinem Peters ◽  
Ole Gemeinhardt ◽  
Stefan M. Niehues ◽  
Friedrich Jung
Keyword(s):  
Cell Culture ◽  
Lactate Dehydrogenase ◽  
Culture Media ◽  
Cell Culture Media ◽  
Ldh Release

scholarly journals A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

2021 ◽  
Vol 22 (18) ◽  
pp. 9896
Author(s):  
Eugenia Romano ◽  
Paolo Antonio Netti ◽  
Enza Torino
Keyword(s):  
Cell Culture ◽  
High Pressure ◽  
Culture Media ◽  
Preliminary Test ◽  
Bilayer Membrane ◽  
Microfluidic System ◽  
Culture Model ◽  
Before And After ◽  
Dynamic High Pressure

In recent decades, endogenous nanocarrier-exosomes have received considerable scientific interest as drug delivery systems. The unique proteo-lipid architecture allows the crossing of various natural barriers and protects exosomes cargo from degradation in the bloodstream. However, the presence of this bilayer membrane as well as their endogenous content make loading of exogenous molecules challenging. In the present work, we will investigate how to promote the manipulation of vesicles curvature by a high-pressure microfluidic system as a ground-breaking method for exosomes encapsulation. Exosomes isolated from Uppsala 87 Malignant Glioma (U87-MG) cell culture media were characterized before and after the treatment with high-pressure homogenization. Once their structural and biological stability were validated, we applied this novel method for the encapsulation in the lipidic exosomal bilayer of the chemotherapeutic Irinotecan HCl Trihydrate-CPT 11. Finally, we performed in vitro preliminary test to validate the nanobiointeraction of exosomes, uptake mechanisms, and cytotoxic effect in cell culture model.

scholarly journals Secretion of glutathione S-transferase isoforms in the seminiferous tubular fluid, tissue distribution and sex steroid binding by rat GSTM1

1999 ◽  
Vol 340 (1) ◽  
pp. 309-320 ◽  
Author(s):  
Sikha Bettina MUKHERJEE ◽  
S. ARAVINDA ◽  
B. GOPALAKRISHNAN ◽  
Sushma NAGPAL ◽  
Dinakar M. SALUNKE ◽  
...  
Keyword(s):  
Cell Culture ◽  
Germ Cell ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Culture Media ◽  
Glutathione S Transferase ◽  
Steroid Binding ◽  
Spent Media

The seminiferous tubular fluid (STF) provides the microenvironment necessary for spermatogenesis in the adluminal compartment of the seminiferous tubule (ST), primarily through secretions of the Sertoli cell. Earlier studies from this laboratory demonstrated the presence of glutathione S-transferase (GST) in STF collected from adult rat testis and in the spent media of ST cultures. This study describes the cellular source, isoform composition and possible function of GSTs in the STF. The major GST isoforms present in STF in vivo share extensive N-terminal similarity with rat GSTM1 (rGSTM1), rGSTM2, rGSTM3 and rGST-Alpha. Molecular masses of rGSTM2, rGSTM3 and rGST-Alpha from liver and testis sources were similar, unlike STF-GSTM1, which was larger by 325 Da than its liver counterpart. Peptide digest analysis profiles on reverse-phase HPLC between liver and STF isoforms were identical, and N-terminal sequences of selected peptides obtained by digestion of the various isoforms were closely similar. The above results confirmed close structural similarity between liver and STF-GST isoforms. Active synthesis and secretion of GSTs by the STs were evident from recovery of radiolabelled GST from the spent media of ST cultures. Analysis of secreted GST isoforms showed that GST-Alpha was not secreted by the STs in vitro, whereas there was an induction of GST-Pi secretion. Detection of immunostainable GST-Mu in Sertoli cells in vitro and during different stages of the seminiferous epithelium in vivo, coupled with the recovery of radiolabelled GST from Sertoli cell-culture media, provided evidence for Sertoli cells as secretors of GST. In addition, STF of ‘Sertoli cell only’ animals showed no change in the profile of GST isoform secretion, thereby confirming Sertoli cells as prime GST secretors. Non-recovery of [35S]methionine-labelled GSTs from germ cell culture supernatants, but their presence in germ cell lysates, confirm the ability of the germ cells to synthesize, but not to release, GSTs. Functionally, STF-GSTM1 appeared to serve as a steroid-binding protein by its ability to bind to testosterone and oestradiol, two important hormones in the ST that are essential for spermatogenesis, with binding constants of < 9.8×10-7 M for testosterone and 9×10-6 M for oestradiol respectively.

Elicitor-induced Changes of Enzyme Activities Related to Isoflavone and Pterocarpan Accumulation in Chickpea (Cicer arietinum L.) Cell Suspension Cultures

1988 ◽  
Vol 43 (7-8) ◽  
pp. 536-544 ◽  
Author(s):  
Susanne Daniel ◽  
Walter Hinderer ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Biochanin A ◽  
Primary Metabolism ◽  
Cicer Arietinum L ◽  
L Cell

The extractable activities of thirteen enzymes of primary and secondary metabolism have been measured in chickpea (Cicer arietinum L.) cell suspension cultures after treatment with an elicitor from the fungus Ascochyta rabiei (Pass.) Lab. The cell culture, derived from the A. rabiei resistant cultivar ILC 3279, constitutively accumulated the isoflavones biochanin A and formononetin together with their 7-O-glucosides and the 7-O-glucoside-6″-malonates. After elicitor application the cells rapidly form the pterocarpan phytoalexins medicarpin and maackiain. Among the enzymes of primary metabolism only the glucose 6-phosphate dehydrogenase exhibited a significant increase in activity with a maximum four hours after application of the elicitor. In phenylpropane metabolism the activities of phenylalanine ammonia lyase and chalcone synthase were enhanced by the elicitor and exhibited highest levels after four hours. In contrast the chalcone isomerase activity was not influenced by the elicitor. A substantial enhancement occurred with the isoflavone 7-O-glucosyltransferase activity eight hours after elicitor application. The results suggest that in this cell culture the elicitor-induced biosynthesis of pterocarpan phytoalexins was accompanied with a rapid and transient increase of those enzyme activities which are located at branching points of related pathways, i.e. pentose phosphate cycle, general phenylpropane metabolism, flavonoid formation and isoflavone conjugation.

scholarly journals Improving the metabolic fidelity of cancer models with a physiological cell culture medium

2019 ◽  
Vol 5 (1) ◽  
pp. eaau7314 ◽  
Author(s):  
Johan Vande Voorde ◽  
Tobias Ackermann ◽  
Nadja Pfetzer ◽  
David Sumpton ◽  
Gillian Mackay ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Culture Media ◽  
Hypoxia Inducible Factor ◽  
Commercial Media ◽  
Physiological Medium ◽  
Cancer Models ◽  
Cancer Spheroids

Currently available cell culture media may not reproduce the in vivo metabolic environment of tumors. To demonstrate this, we compared the effects of a new physiological medium, Plasmax, with commercial media. We prove that the disproportionate nutrient composition of commercial media imposes metabolic artifacts on cancer cells. Their supraphysiological concentrations of pyruvate stabilize hypoxia-inducible factor 1α in normoxia, thereby inducing a pseudohypoxic transcriptional program. In addition, their arginine concentrations reverse the urea cycle reaction catalyzed by argininosuccinate lyase, an effect not observed in vivo, and prevented by Plasmax in vitro. The capacity of cancer cells to form colonies in commercial media was impaired by lipid peroxidation and ferroptosis and was rescued by selenium present in Plasmax. Last, an untargeted metabolic comparison revealed that breast cancer spheroids grown in Plasmax approximate the metabolic profile of mammary tumors better. In conclusion, a physiological medium improves the metabolic fidelity and biological relevance of in vitro cancer models.

Trypanosoma musculi: In vitro cultivation of blood forms in cell culture media

1977 ◽  
Vol 7 (2) ◽  
pp. 109-111 ◽  
Author(s):  
P. Viens ◽  
M.C. Lajeunesse ◽  
R. Richards ◽  
G.A.T. Targett
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
In Vitro Cultivation ◽  
Cell Culture Media

scholarly journals PHAIR – A biosensor for pH measurement in air-liquid interface

2020 ◽  
Author(s):  
Mohammadhossein Dabaghi ◽  
Neda Saraei ◽  
Gang Xu ◽  
Abiram Chandiramohan ◽  
Jonas Yeung ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Silver Chloride ◽  
Reference Electrode ◽  
Airway Epithelial Cells ◽  
Liquid Interface ◽  
Small Scale ◽  
Human Airway ◽  
Air Liquid Interface

1AbstractIn many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR - a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air-liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenging conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wires-based sensors have the capacity for miniaturization, and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings.

Sign in / Sign up

Export Citation Format

Share Document