scholarly journals Identifikasi Bakteri Escherichia Coli pada Air Minum Isi Ulang yang Diproduksi Depot Air Minum Isi Ulang di Kecamatan Padang Selatan

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Fathoni Afif ◽  
Erly Erly ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Microbiological Parameters ◽  
Complete Test ◽  
Biochemistry Test

AbstrakMenurut Permenkes No. 492 tahun 2010, air minum berkualitas dinilai dari parameter mikrobiologi adalah tidak ditemukannya bakteri total coliform dan Escherichia coli dalam 100 ml sampel. Menurut beberapa penelitian sebelumnya, banyak depot air minum isi ulang yang memproduksi air tidak sesuai dengan persyaratan secara mikrobiologi tersebut. Tujuan penelitian ini adalah untuk mengidentifikasi kehadiran bakteri Escherichia coli pada air minum yang diproduksi depot air minum isi ulang di Kecamatan Padang Selatan. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas pada sampel air yang didapat dari 13 depot yang tidak melakukan uji kualitas. Jenis penelitian ini adalah deskriptif. Data diperoleh dengan cara observasi dan wawancara langsung. Penelitian mikrobiologi air menggunakan metode Most Probable Number (MPN) yang terdiri dari presumptive test dengan menggunakan media Lactose Broth (LB) dengan metode 3 tabung, confirmative test dengan menggunakan media Brillian Green Lactose Broth (BGLB), complete test dengan menggunakan media endo agar, dan tes identifikasi dengan menggunakan media uji biokimia. Hasil penelitian didapatkan bahwa 10 dari 13 sampel tercemar oleh bakteri coliform dan 2 sampel memenuhi syarat untuk penelitian selanjutnya secara kualitatif. Pada penelitian lanjutan, tidak ditemukan bakteri Escherichia coli namun ditemukan bakteri Pseudomonas aeruginosa.Kata kunci: escherichia coli, depot air minum isi ulang, MPN AbstractAccording to the Minister Regulation. 492 in 2010, assessed quality of drinking water in microbiological parameters is by the discovery of total coliform bacteria and Escherichia coli in a 100ml sample. According to some previous studies, many drinking water that produced by the depot does not conform to the microbiological requirements The objective of this study was to identify the presence of the Escherichia coli in drinking water produced by refill drinking water depot in the District of South Padang. Research had been carried out in the Laboratory of Microbiology, Faculty of Medicine, University of Andalas in water samples obtained from 13 depots that don’t test the quality. This research was a descriptive. Data obtained by direct observation and interviews. This research used Most Probable Number (MPN) test for the microbiological of the water that consist of presumptive test using Lactose Broth (LB), confirmative test using Brilliant Green Lactose Broth (BGLB), complete test using endo jelly media, and identification test using biochemistry test. The results showed that 10 of 13 samples contaminated by coliform bacteria and 2 samples eligible for further research that show there is a Pseudomonas aeruginosa.Keywords: escherichia coli, refill water depot, MPN

Microbial Quality of Bagged Baby Spinach and Romaine Lettuce: Effects of Top versus Bottom Sampling

2012 ◽  
Vol 75 (1) ◽  
pp. 132-136 ◽  
Author(s):  
JULIE A. KASE ◽  
STACEY BORENSTEIN ◽  
ROBERT J. BLODGETT ◽  
PETER C. H. FENG
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Microbial Quality ◽  
Romaine Lettuce ◽  
Probable Number ◽  
E Coli ◽  
Bottom Sampling

Contamination with Escherichia coli O157:H7 and Salmonella have called into question the safety and microbial quality of bagged ready-to-eat leafy greens. This study expands on previous findings that these goods have high total bacteria counts (TBC) and coliform counts, variation in counts among different lots, that Escherichia coli is present, and disparities in counts when bags are top or bottom sampled. Nearly 100 bags of baby spinach and hearts of romaine lettuce from a single brand were subjected to both top and bottom sampling. Product was blended, and a portion serially diluted and plated to obtain TBC. Total coliform and E. coli levels were estimated by the most-probable-number (MPN) technique with ColiComplete discs. Top-sampled TBC from bags of baby spinach (48 bags, 13 different lots) ranged from 3.9 to 8.1 log CFU/g and bottom-sampled TBC ranged from 4.0 to 8.2 log CFU/g, with 52% of the bags (or 39% of the lots) producing TBC higher in bottom samples. For hearts of romaine (47 bags from 19 different lots), top-sampled bags had TBC ranging from 2.4 to 7.0 log, and bottom-sampled bags had TBC from 3.3 to 7.3 log, with 64% of the bags (or 63% of the lots) showing higher TBC in bottom samples. However, we are unable to reject the hypothesis that the top and bottom samples from either commodity contain the same TBC (P ≥ 0.08). No E. coli was detected and total coliform bacteria counts were, with few exceptions, ≥210 MPN/g, irrespective of TBC. In general, lots with the most number of days before the printed “use-by” date had lower TBC. However, the R2 values for either baby spinach (0.4085) or hearts of romaine (0.2946) suggest that age might not be a very good predictor of higher TBC. TBC varied widely between lots and even more so within same-lot samples, as indicated by the sum of squares results. This finding, along with higher TBC in bottom samples, suggests further consideration when a microbiological sampling scheme of bagged produce is designed.

scholarly journals Kualitas Air Minum Yang Diproduksi Depot Air Minum Isi Ulang Di Kecamatan Bungus Padang Berdasarkan Persyaratan Mikrobiologi

2012 ◽  
Vol 1 (3) ◽  
Author(s):  
Rido Wandrivel ◽  
Netty Suharti ◽  
Yuniar Lestari
Keyword(s):  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Morbidity Rate ◽  
Probable Number ◽  
E Coli ◽  
Quality Of Water ◽  
Complete Test ◽  
Quality Water

Abstrak Latar belakang: Pendahuluan: Setiap depot air minum wajib melakukan pemeriksaan mutu produk sesuai dengan peraturan yang berlaku, namun tidak satupun dari sembilan depot air minum di Kecamatan Bungus melakukan hal tersebut. Diare adalah salah satu penyakit yang disebabkan oleh air minum yang tidak berkualitas. Morbiditas rate diare di Kecamatan Bungus tertinggi ke-2 di Kota Padang. Penelitian ini bertujuan untuk mengetahui kualitas air minum yang dihasilkan depot air minum di Kecamatan Bungus berdasarkan persyaratan mikrobiologi. Metode Penelitian: Populasi pada penelitian ini adalah air minum yang berasal dari depot air minum isi ulang di Kecamatan Bungus Padang dan sampel pada penelitian ini adalah seluruh populasi yang ada, yaitu sembilan sampel. Penelitian dilaksanakan dalam 2 tahap yaitu pengambilan sampel air dengan galon air sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologis dengan Most Probable Number Test (MPN) terhadap sampel yang terdiri dari tiga tes, yaitu presumptive test, confirmative test, dan complete test. Hasil Penelitian: Lima dari sembilan sampel mengandung bakteri Coliform dan tiga dari lima sampel tersebut juga mengandung E. coli. Kesimpulan: Hal ini menunjukkan bahwa 55,6% depot air minum di Kecamatan Bungus menghasilkan air minum yang kualitasnya tidak memenuhi persyaratan mikrobiologi yang telah ditetapkan pemerintah. Beberapa faktor yang dapat mempengaruhi adalah air baku, kondisi depot, kebersihan operator, dan penanganan terhadap wadah pembeli. Kata kunci: Depot air minum isi ulang, pemeriksaan mikrobiologis Abstract Background: Every depot is obliged to do inspection of product quality prescribed by the regulations, but not one even also from nine drinking water depot in District of Bungus do the mentioned. Diarrhea is one of the disease that cause by bad quality water product. Diarrhea morbidity rate in the Bungus District is second highest in Padang City. This Research aim to to know the drinking water quality produced by drinking water refill depot in the Bungus District based on microbiological requirements. Methods: Population of this research is drinking water refill depots in the Bungus District of Padang city and sample of this research is all of existing population, that is nine drinking water depot. Research executed in 2 phase that is intake of water sample with gallon at the same time do observation of the factor that influencing the quality of water and mikrobiological test with Most Probable Number Test ( MPN) to the sampel which consist of three test, that is presumtive test, konfirmative test, and complete test. Result: Five from nine sample contain coliform bacteria and three from five the sampel also contain E. coli. Conclusion: This matter indicate that 55,6% drinking water refill depot in District of Bungus produce drinking water which its quality don’t fulfill microbiological requirements which have been specified by government. Some factor able to influence is standard water, condition of depot, hygiene of operator, and handling to place of buyer. Keywords: Drinking water refill depots, mikrobiological test

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals KUALITAS BAKTERIOLOGIS AIR MINUM ISI ULANGPADA TINGKAT PRODUSEN DI KABUPATEN BADUNG.

2015 ◽  
Vol 9 (2) ◽  
pp. 52
Author(s):  
Made Partiana ◽  
Made Sudiana Mahendra ◽  
Wayan Redi Aryanta
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Significant Relationship ◽  
Raw Materials ◽  
Urban Community ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Bacteriological Examination ◽  
Probable Number

The presence of refill drinking water in the urban community especially Badung Regency is favored as an alternative of water supply because it cost relatively cheaper compared to water in pacage. The number of samples in this study was 45 outlets with refill drinking water as its study material. The bacteriological examination of the material of study was done on included most probable number (MPN) of coliform and Eschericia coli. The result of refill drinking water investigation bacteriological examination revealed 88.9 % outlets were considered qualified, 11.1 % of them were unqualified because they contained coliform bacteria and one stall had Eschericia coli. The observation hygiene 15.6 % unqualified and sanitation outlets examination 17.8 % unqualified. Correlation test showed no significant relationship between raw materials, equipment, processing, hygiene and sanitation autlets with bacteriological quality of drinking water

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

scholarly journals Contamination of Escherichia coli Drinking Water Refills on Drinking Water Depots in Malang City

2019 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Wiwik Kusmawati ◽  
Lia Rahayu
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
The City ◽  
Maximum Limit

Data from the Malang City Health Office shows the increasing number of refill drinking water depots in the city of Malang until 2013 there were 163 drinking water depots scattered in each Malang Subdistrict. The purpose of this study was to determine the contamination of Escherichia coli refill drinking water in drinking water depots in Malang. The samples taken were 20 refill drinking water depots in Malang. Sampling is done by simple random sampling. Escherichia coli examination using the Most Probable Number Test. Of the 20 samples tested, 2 samples met the requirements of drinking water quality according to PERMENKES and 18 other samples containing the number of coliform bacteria exceeding the maximum limit of 0 per 100 ml of water. There were Escherichia coli in 10 samples while 8 other samples contained other coliform bacteria.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

scholarly journals Microbiological quality analysis of fresh vended fruit juices and water sold in roadside stalls in Dhaka Metropolis by MPN method

2017 ◽  
Vol 7 (1) ◽  
pp. 1-6
Author(s):  
Mahamuda Akther Eva ◽  
Shawda Shafiq Shreya ◽  
Tasnia Ahmed
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Microbiological Quality ◽  
Test Method ◽  
Quality Analysis ◽  
Most Probable Number ◽  
Gram Negative Bacteria ◽  
Probable Number ◽  
Gram Negative

Quality of drinking water and juice is very important because if the quality deteriorates by the contamination of faecally originated microorganisms, it may cause serious diarrhea associated problems leading to death. In overpopulated countries like Bangladesh, this is a common scenario to experience diarrheal diseases due to drinking non-potable water as well as contaminated fresh juices. Present study was conducted to determine the quality of drinking water and juice by detection of indicator bacteria Escherichia coli by MPN (Most Probable Number) method which was performed by three consecutive steps including presumptive test, confirmed test and completed test. Other gram negative bacteria were also identified by biochemical methods. The indicator bacterium Escherichia coli was detected in two water samples out of 15 samples and one juice sample out of fifteen samples respectively during the MPN test method. Other Gram negative bacteria found in both water and juice samples included Klebsiella spp., Alcaligenes spp., Pseudomonas spp. and Proteus spp. The quality of drinking water and juice was found to be good in Bangladesh but proper hygiene should be maintained more strictly to avoid the contamination by other gram negative bacteria which are also capable of causing disease. Stamford Journal of Microbiology, Vol.7(1) 2017: 1-6

scholarly journals Escherichia Coli Content in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency

2020 ◽  
Vol 7 (2) ◽  
pp. 6
Author(s):  
Azwar Azwar ◽  
Safrida Safrida ◽  
Yarmaliza Yarmaliza ◽  
Fitrah Reynaldi ◽  
Arfah Husna
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
World Health ◽  
Most Probable Number ◽  
Cross Sectional ◽  
Probable Number ◽  
E Coli ◽  
Escherichia Coli Bacteria

The quality of drinking water supply must be guaranteed. The WHO (World Health Organization) determines water quality by means of E. Coli bacteria. Based on the Minister of Health Regulation No. 492 / MENKES / PER / IV / 2010 The maximum permissible content of E. Coli in drinking water is 0 per 100 ml. The presence of E. coli in water is an indicator of human and animal fecal pollution that can cause diarrhea and even death. Selection of Refillable Drinking Water (AMIU) as an alternative to fulfill drinking water needs is a risk that can endanger health if the quality of AMIU is added if consumers do not pay attention to safety and hygiene. This study aims to see the content of E. coli in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency. This research is experimental with cross sectional design using quantitative laboratory examination with the Most Probable Number (MPN) method. The population in this study were all refill drinking water depots that did not have a drinking certificate in Samatiga District, West Aceh Regency. The research sample used a total sampling method of 7 refill drinking water depots. The results of the analysis showed that from all samples as many as 6 samples (85.71%) tested positive for Escherichia coli bacteria and 1 sample (14.29%) tested negative for Escherichia coli bacteria. Based on the calculation of the number of colonies with the MPN / 100 ml table then comparing with the requirements of the Regulation of the Minister of Health of the Republic of Indonesia Number 492 / Menkes / Per / IV / 2010 concerning drinking water quality requirements that the 6 samples tested positive were not suitable for consumption.

Sign in / Sign up

Export Citation Format

Share Document