scholarly journals Escherichia Coli Content in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency

2020 ◽  
Vol 7 (2) ◽  
pp. 6
Author(s):  
Azwar Azwar ◽  
Safrida Safrida ◽  
Yarmaliza Yarmaliza ◽  
Fitrah Reynaldi ◽  
Arfah Husna
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
World Health ◽  
Most Probable Number ◽  
Cross Sectional ◽  
Probable Number ◽  
E Coli ◽  
Escherichia Coli Bacteria

The quality of drinking water supply must be guaranteed. The WHO (World Health Organization) determines water quality by means of E. Coli bacteria. Based on the Minister of Health Regulation No. 492 / MENKES / PER / IV / 2010 The maximum permissible content of E. Coli in drinking water is 0 per 100 ml. The presence of E. coli in water is an indicator of human and animal fecal pollution that can cause diarrhea and even death. Selection of Refillable Drinking Water (AMIU) as an alternative to fulfill drinking water needs is a risk that can endanger health if the quality of AMIU is added if consumers do not pay attention to safety and hygiene. This study aims to see the content of E. coli in Refill Drinking Water (AMIU) in Samatiga District, West Aceh Regency. This research is experimental with cross sectional design using quantitative laboratory examination with the Most Probable Number (MPN) method. The population in this study were all refill drinking water depots that did not have a drinking certificate in Samatiga District, West Aceh Regency. The research sample used a total sampling method of 7 refill drinking water depots. The results of the analysis showed that from all samples as many as 6 samples (85.71%) tested positive for Escherichia coli bacteria and 1 sample (14.29%) tested negative for Escherichia coli bacteria. Based on the calculation of the number of colonies with the MPN / 100 ml table then comparing with the requirements of the Regulation of the Minister of Health of the Republic of Indonesia Number 492 / Menkes / Per / IV / 2010 concerning drinking water quality requirements that the 6 samples tested positive were not suitable for consumption.

scholarly journals Effect of Good Hygiene Sanitation and Personal Hygiene Depot Management of Refill Drinking Water on the Quality of Drinking Water Reviewed from Most-Probable-Number Coliform in Kediri

2018 ◽  
Vol 54 (4) ◽  
pp. 241
Author(s):  
Indasah Indasah
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Personal Hygiene ◽  
Most Probable Number ◽  
Cross Sectional ◽  
Probable Number ◽  
Hygiene Management

Drinking water is a basic human need and must meet health requirements. Drinking water depots are a means of filling drinking water which is in the process of processing raw water using treatment and sterilization system. Drinking water depots as providers of drinking water must also meet the requirements of sanitation hygiene and personal hygiene management must also be maintained so that the water produced meets health requirements. The purpose of this study was to determine the effect of sanitation hygiene and personal hygiene management of refill drinking water depots on the quality of drinking water in terms of the Most Probable Number Coliform in Kediri. The research design used was analytic observational with cross sectional approach. The population of all refill drinking water depots in Kediri is 57, with the sampling technique that is Simple Random Sampling, where a portion of refill drinking water depots in Kediri are 50. Data is collected by check list, questionnaire and laboratory examination. The results showed that most of the hygiene sanitation of the drinking water depot had fulfilled the requirements of 77%, the drinking water personal hygiene depot was good as much as 85% and those who met the drinking water requirements were 70%. The results of the hygiene feasibility analysis showed the value of Sig 0.000<0.05 (R=0.935) means that there was an effect of hygiene sanitation quality with water quality in terms of the Most Probable Number Coliform. While the personal hygiene value of Sig 0.001 <0.05 (R = 0.528) indicates that there is a personal hygiene influence with water quality in terms of the Most Probable Number Coliform. The results of Logistic Regression test showed that there were effects of sanitation hygiene and personal hygiene management of refill drinking water depots with water quality in terms of the Most Probable Number Coliform with a significance value = 0.000 <a 0.05. This shows that the hygiene and the better personal hygiene at refill drinking water depots is increasingly fulfilling water quality requirements in terms of the Most Probable Number Coliform

scholarly journals Bacteriological Quality of Drinking Water in Mardan, Khyber Pakhtunkhwa, Pakistan

2015 ◽  
Vol 21 ◽  
pp. 1-6
Author(s):  
Javid Ali ◽  
Said Hassan ◽  
Dr Ziaurahman ◽  
Inayat Ur Rahman ◽  
Sadhair Abbas ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Most Probable Number ◽  
Probable Number ◽  
Khyber Pakhtunkhwa ◽  
E Coli ◽  
Micro Organisms

The present study was aimed to isolate and identify micro-organisms load of drinking water of Mardan city, Khyber Pakhtunkhwa Pakistan. A total of 27 samples of drinking water were collected from different locations of the study area. Total Plate Count was determined by pour plate method, while total coliforms, total fecal coliforms and E. coli were determined by multiple tube fermentation method. Of the total collected samples, 17 (62.96%) samples were contaminated with either one or more than one type of microorganisms. The results of most probable number test showed that 13 (48.15%) samples were unsatisfactory. It was concluded that the water should be treated before consumption for drinking purpose. Regular assessment of the water quality is recommended as regular monitoring of the water quality for improvement not only prevents disease and hazards but also checks the water resources from becoming further polluted. ECOPRINT 21: 1-6, 2014DOI: http://dx.doi.org/10.3126/eco.v21i0.11897

scholarly journals GREEN SYNTHESIS OF MAGNETIC IRON NANOPARTICLES USING MEDICINAL PLANT TRIDAX PROCUMBENS LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIMICROBIAL AGENT AGAINST E. COLI

2020 ◽  
pp. 34-39
Author(s):  
YOJANA Y. PATIL ◽  
VAISHNVI B. SUTAR ◽  
ARPITA P. TIWARI
Keyword(s):  
Escherichia Coli ◽  
Magnetic Nanoparticles ◽  
Iron Nanoparticles ◽  
Most Probable Number ◽  
Leaf Extracts ◽  
Probable Number ◽  
Gram Negative ◽  
E Coli ◽  
Tridax Procumbens ◽  
Escherichia Coli Bacteria

Objective: The present study was aimed at the biological synthesis of magnetic iron nanoparticles by using the plant extract of Tridax procumbens and also to study their antimicrobial property against gram-negative bacteria (Escherichia coli). Methods: The synthesis of magnetic iron nanoparticles was carried out by the co-precipitation method using biological methods like plant extract as reducing agent and capping agents are biocompatible and non-hazardous. These nanoparticles were characterized by UV-Visible spectroscopy, XRD (X-Ray Diffraction), and SEM (Scanning Electron Microscope). As well as antibacterial activity of the nanoparticles was carried out by agar well diffusion method and Most Probable Number (MPN) method against gram-negative E. coli (Escherichia coli) bacteria. Results: The average crystallite size of Magnetic Nanoparticles (MNPs) was found to be 72 nm by X-ray diffraction. The optical absorption band at wavelengths of 240 nm and 402 nm was obtained from the UV Visible spectrum. Spherical shape morphology was observed in SEM studies. The antibacterial assay clearly expressed that E. coli showed a maximum zone of inhibition (15±0.15 mm) at 2 mg/ml and 1 mg/ml concentration was found for Magnetic Nanoparticles. In the Most Probable Number (MPN) test it is seen that the bacterial count is reduced after adding synthesized NPs into the water sample. Conclusion: The results of the present study conclude that the Magnetic Nanoparticles synthesized using Tridax procumbens leaf extracts is found to be stable and show good antibacterial activity against gram-negative (Escherichia coli) bacteria.

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

scholarly journals Isolation and Enumeration of Escherichia coli from Soil and Water

2020 ◽  
Vol 36 (2) ◽  
pp. 75-77
Author(s):  
Anindita Bhowmik ◽  
Sunjukta Ahsan
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Gas Production ◽  
Soil Samples ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
E Coli ◽  
Dilution Series

Majority of the population of Bangladesh depend on tap or surface water as their source of water supply. This study was carried out to examine the microbial quality of both water and soil collected from different places using the multiple tube fermentation technique to determine coliform count by the most probable number (MPN) method in brilliant green lactose broth (BGLB) media.Inoculum from positive tubes of the presumptive test were further transferred on eosinemethylene blue (EMB) and MacConkey agar.The organisms isolated were further characterized using biochemical tests. Out of 93 water samples, 30 (32.26%) indicated the presence of lactose fermenter and gas producer in all 3 tubes of dilution series using inoculum quantities of 1.0, 0.1 and 0.01 ml, whereas out of 85 soil samples, 45 (52.94%) showed acid and gas production in all 3 tubes of dilution series.Among 85 soil samples, 40 samples that contained at least one positive in each dilution series and among 93 water samples, 31 samples that contained at least one positive in each dilution series were further re-identified with biochemical tests.This study showed 30.59% soil isolates and 26.88% water isolates were Escherichia coli which highlighted the fact that both water and soil act as a major reservoir of E.coli, which indicates possible fecal contamination as well as presence of potentially pathogenic E. coli. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 75-77

Bacterial quality of drinking water sources and antimicrobial resistance profile of Enterobacteriaceae in Bahir Dar city, Ethiopia

2014 ◽  
Vol 4 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Bayeh Abera ◽  
Mulugeta Kibret ◽  
Goraw Goshu ◽  
Mulat Yimer
Keyword(s):  
Drinking Water ◽  
Antimicrobial Resistance ◽  
Water Samples ◽  
Multiple Drug Resistance ◽  
Tap Water ◽  
World Health ◽  
Most Probable Number ◽  
Bahir Dar ◽  
Probable Number

A cross-sectional study was conducted to determine the bacterial quality and antimicrobial susceptibility profiles of Enterobacteriaceae from drinking water in Bahir Dar city. A total of 140 water samples were collected in the wet and dry periods from springs (n = 4), reservoirs (n = 10) and private tap water at households (n = 126). Bacteriological analysis of water was conducted using multiple tube method. Overall, 21.4%, 18.6% and 17.8% of drinking water samples had total coliforms (TC), faecal coliforms (FC) and Escherichia coli, respectively. All spring water samples and 29.2% of private tap water had the highest TC load (18 most probable number/100 mL, 95% CI: 100). For FC, 81.4% of the drinking water supplies tested complied with both World Health Organization and Ethiopian Standards. High levels of resistance (98–100%) were observed for ampicillin by Enterobacteriaceae and Pseudomonas aeruginosa. All P. aeruginosa isolates and 20 (66.7%) of E. coli revealed multiple drug resistance. Enterobacteriaceae and P. aeruginosa isolates exhibited high levels of antimicrobial resistance. The bacterial quality of drinking water in Bahir Dar city was poor. Microbial surveillance and monitoring with periodic assessment on physical integrity of the water pipelines need to be undertaken.

scholarly journals The rationale for introducing the indices «Generalized coliform bacteria» and «Escherichia coli» into the scheme of sanitary and microbiological control of water quality as indices of fecal contamination

2021 ◽  
Vol 99 (12) ◽  
pp. 1353-1359
Author(s):  
Angelika V. Zagainova ◽  
Galina M. Trukhina ◽  
Yury A. Rakhmanin ◽  
Tamara Z. Artemova ◽  
Marina A. Sukhina
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Contamination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Intestinal Infections ◽  
Safety Of Drinking Water ◽  
Microbiological Indicators

Introduction. The increasing bacterial contamination of water bodies requires an increase in water quality control’s reliability to ensure epidemic safety against waterborne infections. Therefore, researchers in both Russia and Europe came to the conclusion that it is necessary to search for indicator microorganisms that can more accurately suggest the presence of pathogens. microorganisms in water than traditional indicators. The aim of the study was to justify the introduction of indicator indices of fecal contamination “generalized coliform bacteria” and Escherichia coli to assess the safety of drinking water Material and methods. The article provides an analysis of domestic and international regulatory documents and literary materials regulating the quality of drinking water in terms of sanitary and microbiological indicators and assessment criteria. The results of many years of experimental and field research carried out by research organizations and practical organizations of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare are presented. Results. On the territory of Russia, “general coliform bacteria,” is one of the indicator indices chosen according to the fermentation of lactose, determine the safety of drinking water. The water does not take into account pathogenic bacteria (Salmonella, Shigella) and a number of lactose-negative opportunistic bacteria, causative agents of intestinal infections. The study of microorganisms isolated from the feces of patients confirms the frequency of occurrence of lactose-negative microorganisms to varying from 20 to 100% of strains. With an annual trend towards a decrease in the percentage of non-standard drinking water samples in terms of microbiological indices, general intestinal infections (GII) of unknown etiology increase, i.e. risk of GII. If the quality of drinking water does not correspond to thermotolerant coliform bacteria (TCB), 95% of samples contain E. coli. Therefore, the determination of E. coli more reliably indicates the intake of fresh fecal contamination and provides efficiency in taking measures to eliminate an unfavorable situation than TCB. Conclusion. Reasons are given for the introduction of more reliable microbiological indicators of water safety control, such as - “generalized coliform bacteria” with the preservation of the abbreviation GCB, combining both lactose-positive and lactose-negative bacteria, determined by the sign of glucose fermentation, negative oxidase test and negative stain according to Gram and E. coli as an indicator of recent faecal contamination, which will allow the assessment of water quality for a wide range of bacteria of the order Enterobacterials, corresponding to the modern taxonomy of Enterobacteriaceae NCBI, will ensure harmonization with international requirements and the safety of drinking water for the population.

scholarly journals Contamination of Escherichia coli Drinking Water Refills on Drinking Water Depots in Malang City

2019 ◽  
Vol 7 (1) ◽  
pp. 9
Author(s):  
Wiwik Kusmawati ◽  
Lia Rahayu
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
The City ◽  
Maximum Limit

Data from the Malang City Health Office shows the increasing number of refill drinking water depots in the city of Malang until 2013 there were 163 drinking water depots scattered in each Malang Subdistrict. The purpose of this study was to determine the contamination of Escherichia coli refill drinking water in drinking water depots in Malang. The samples taken were 20 refill drinking water depots in Malang. Sampling is done by simple random sampling. Escherichia coli examination using the Most Probable Number Test. Of the 20 samples tested, 2 samples met the requirements of drinking water quality according to PERMENKES and 18 other samples containing the number of coliform bacteria exceeding the maximum limit of 0 per 100 ml of water. There were Escherichia coli in 10 samples while 8 other samples contained other coliform bacteria.

scholarly journals Preliminary Assessment of Water Quality in Some Hydrocarbon-imparted Ogoni Communities in River State, Nigeria

2021 ◽  
pp. 88-97
Author(s):  
Caroline Barituka Ganabel ◽  
Confidence Kinikanwo Wachukwu ◽  
Samuel Douglas Abbey ◽  
Easter Godwin Nwokah
Keyword(s):  
Water Quality ◽  
Heavy Metal ◽  
Drinking Water ◽  
Water Samples ◽  
Physicochemical Parameters ◽  
Pearson Correlation ◽  
Statistical Significance ◽  
World Health ◽  
Most Probable Number ◽  
Probable Number

Aim: This study aimed to assess the quality of drinking water in some hydrocarbon-impacted Ogoni communities. Study Design: The study employ a cross-sectional and analytical design using stratified sampling method. Place and Duration of Study: Department of Medical Laboratory Science of Rivers State University, Giolee Global Resource Limited and Environmental Consultancy Services between March 2020 and March 2021. Methodology: Water samples were collected from (20 hydrocarbon- impacted communities) in the 4 Local Government Area (LGAs) of Ogoni land. These water samples were analyzed to determine the physicochemical, bacteriological, heavy metal and total petroleum hydrocarbon (TPH) parameters using standard methods and operational procedures. The data obtained were subjected to descriptive statistical analysis. The general linearized model (GIG) was used to generate analysis of variance (ANOVA) mean and standard error and arrange, statistically significant was set as p-value of .05 (95% confidence limit). Pearson correlation test was used to calculate the correlation between TPH, Heavy metal, and physicochemical parameters in hydrocarbon and non-hydrocarbon impacted communities. All statistical analyses were performed using GraphPad Prism (Version 8). Results: The results obtained for physicochemical parameters were pH 4.3±0.8 mg/l, EC 0.03±0.05 mg/l, DO 5.5±1.6 mg/l, Temp 25.0±0.0 mg/l, Mv 0.32±0.27 mg/l, Nitrite 0.0058±013 mg/l, Nitrate 0.1530±158 mg/l. These results were all below the recommended standard for Nigeria standard of drinking water quality (NSDWQ) and World Health Organization (WHO). The bacteriological analyses were carried out using multiple tubes technique (Most Probable Number), total coliform count, (TC), total heterotrophic count (THBC) and total fungal count (TFC). The results obtained were 0.941±2.397 cfu/ml, 89.3±176.6 cfu/ml, 297.8±144.4 cfu/ml, and 0.32±0.84 cfu/ml respectively. The p-values for TFC (<0.0002) were statistically significant. Heavy metal profiling was: Cr 0.194±0.320 mg/l, Cd 0.469±0.569 mg/l, Cu 0.211±0.348 mg/l, Pb 0.0336±0.20 mg/l, Fe 0.705±1.244 mg/l, Zn 0.258±0.249 mg/l, respectively. Generally, the concentration of heavy metal   increased more than the standard recommended by NSDWQ and WHO except for Zn 0.255±0.249 mg/l, and Cu 0.56±0.50 mg/l, that is slightly lower than the acceptable limit recommended by WHO and NSWWQ. The sequences of heavy metal concentration were in Cd > Cr> Pb>Fe> Cu.>Zn. The statistical significance values for Pb p=.003 and for Zn p=.009 were statistically significant. The concentration of TPH were (349.9 ppm/ml) higher than the recommended values for NSDWQ and WHO. Conclusion: The findings in this research reviewed a worrisome level of TPH and Pb, and which could have devastating impact on bacterial biodiversity.

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